Fig. 4

Data Normalization module. After entering the data, it is recommended to indicate the list of housekeeping genes that you used (A). You must indicate that you have a list of genes (1), and new question bars will be opened (B). Here you should describe all candidate housekeeping genes separated by spaces (2) and request their evaluation (3). After observing the stability values (C), you can request a default normalization of your data using the best combination of housekeeping genes (4). Since you have normalized your data (D), you can download a table with the normalized −ΔCt values (5) or move on to the next modules