Fig. 5

Expression Analysis module. This module automatically receives the normalized data and update the option questions with your data (A). To run the expression analysis, you should confirm the variable that will be compared (1) and define the groups that will be analyzed (including the reference group) and the gene of interest (2). You can choose to run a parametric or non-parametric test (3). However, it is suggested that the system make this choice based on your data distribution. After defining all the input information, you can run the analysis (4). This module will show a normality analysis of the gene expression for each group (B), boxplots showing −ΔCt (C) and 2^(−ΔΔCt) (D) data by primary groups, and a table summarizing all results generated for the selected gene (E). Red arrows show the respective buttons for downloading boxplots and the results table