Fig. 6

Volcano Plots module. (A) Input parameters required for the analysis. For this module, it is necessary to choose two different groups (1 and 2), define the type of analysis (3), set the type of p value to plot (4), and set the Fold Change (FC) (5) and p value (6) thresholds. Once all input information is defined, you can start the analysis (7). After estimating all fold changes and p values, the system will show the volcano plot (B) and the summary table (C). The volcano plot shows one point per analyzed gene, whose position depends on the log₂ of its fold change and the -log₁₀ p value. Considering the parameters set in panel A, the volcano plot shows labels only for all differentially expressed genes. A color legend is included to classify genes according to the fold change and p value levels. Red arrows show the respective buttons for downloading boxplots and the results table