Fig. 1

Processing ribo-seq data. A Ribo-seq involves experimentally generating reads (top), then computationally mapping sequenced reads to precise ribosome positions (bottom). Ideal data shows a clear triplet periodicity and can be offset to correspond to a position within the P-site of the ribosome by shifting the 5’ or 3’ end of all reads. B Realigned ribo-seq data from Wu et al [38] shows clear triplet periodicity in aggregate across all annotated ORFs in S. cerevisiae (top). This periodic pattern is not as obvious in individual ORFs (bottom). The ribo-seq signal shown here for PDA1, a housekeeping gene commonly used as a reference in gene expression studies, is representative of the signal observed across other genes in S. cerevisiae