Table 2 primerJinn output for eight drug resistance conferring gene regions of M. tuberculosis H37Rv (NC_000962.3)
Primer pair | Forward primer (5′->3′) | Reverse primer (5′->3′) | Forward Tm (°C) | Reverse Tm (°C | Product Size (bp) | Name | Forward Primer Tm NEB (°C) | Reverse Primer Tm NEB (°C) |
|---|---|---|---|---|---|---|---|---|
1 | GAGGAACACCACTAGTACCG | CTCGATGACTTTACGGCCAT | 65 | 64 | 675 | Target 1,461,045–1,461,291 | 64 | 64 |
2 | CATGGGATATGGAGCGATCG | GGGGTCGTAGGAGATCTTGA | 66 | 66 | 791 | Target 490,900–491,416 | 65 | 65 |
3 | CCGGTTGTCCATTCCGTTTA | CTGTACGTATTTGGGTTGCG | 64 | 66 | 454 | Target 1,303,831–1,303,911 | 65 | 64 |
4 | GGATGCGAGCTATATCTCCG | AATACGCCGAGATGTGGATG | 65 | 65 | 458 | Target 1,674,182–1,674,222 | 65 | 65 |
5 | CAACAGTTCATCCCGGTTCG | GACGGATTTGTCGCTCACTA | 65 | 67 | 759 | Target 2,288,681–2,289,242 | 66 | 64 |
6 | GCCACCATCGAATATCTGGT | GCTCCAGGAAGGGAATCATC | 66 | 65 | 778 | Target 761,007–761,277 | 64 | 65 |
7 | GCATACCGAACGTCACAGAT | ACGGTCACCTACAAAAACGG | 66 | 65 | 665 | Target 778,990–779,488 | 65 | 65 |
8 | GCTCTTAAGGCTGGCAATCT | CGGTCACACTTTCGGTAAGA | 65 | 66 | 577 | Target 2,154,831–2,154,873 | 65 | 64 |