Fig. 1

MutAmore pipeline. The tool generates mutated (all possible SAVs) versions of the input sequences and predicts 3D structure for each, e.g., using ColabFold [33] or ESMFold [6]. Optionally, experimental structures for mutants can be input as an alternative to predictions. MutAmore computes the structural difference between each mutant and the predicted wild-type structure and renders mutation profiles along with 3D visualizations. After merging both, the tool renders the final protein mutation movie (PMM). MutAmore can be run in a two-step process, e.g., predicting structure (orange) on a server machine and running the rendering steps (blue) on a desktop machine