Fig. 2

Single frame of protein mutation movie (PMM). Left panel: 3D visualization of the prediction for one single amino-acid variant (SAV). The respective SAV is indicated in the top-left corner (residue position 83: native lysin (K) mutated into isoleucine (I)) and the affected residue is rendered in black in the visualization. All other residues are colored by the AlphaFold2 predicted confidence in the AlphaFold2 color scheme (blue: high confidence; yellow–red: low confidence). Right panel: the mutation profile shows the structural difference between mutant and wild-type structure with residue indices running along the vertical axis and substitution amino-acids on the horizontal. The currently displayed SAV in each frame is indicated by a black border. The movie frame shown was rendered in a screen resolution of 3840 × 2160