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Table 1 ROAST performance in identifying and fixing different types of simulated errors using simulated RNA-seq data

From: Roast: a tool for reference-free optimization of supertranscriptome assemblies

Organism

Incomplete supertranscript

Fragmented supertranscript

False chimera

Local mis-assembly

Missing sequence

Unsupported insertion

Trans-location

Inversion

Human

174 (87)

194 (97)

174 (87)

48 (96)  

50 (100)  

49 (98)

49 (98)

Mouse

172 (86)

196 (98)

167 (84)

50 (100)

49 (98)  

48 (96)

49 (98)

Chicken

179 (90)

196 (98)

176 (88)

50 (100)

49 (98)  

49 (98)

46 (92)

Rice

193 (97)

196 (98)

168 (84)

50 (100)

50 (100)

46 (92)

48 (96)

Arabidopsis

192 (96)

192 (96)

169 (85)

50 (100)

49 (98)  

46 (92)

49 (98)

  1. Percentages are shown in parentheses. A total of 200 errors were produced for each error type including partial and fragmented supertranscripts and false chimeras. For local mis-assemblies, 50 errors were produced for each error type