Fig. 2
From: Introducing the f0% method: a reliable and accurate approach for qPCR analysis
Flow chart of the analysis process. The first step is to calculate the starting efficiency (E). If there is data for a standard curve, approximate f0% is calculated using the fixed E mode of Eq. (3) assuming (E = 2). Then, log10(f0%) is regressed on log10(conc.) and E is calculated from the slope of this regression according to Eq. (6). If there is no data for a standard curve, E is estimated using the free E mode of Eq. (3) and averaged per amplicon. The next step is calculating f0 using the fixed E mode of Eq. (3) utilizing E calculated in the previous step. Followed by calculating f0% using Eq. (5)