Fig. 3
From: Introducing the f0% method: a reliable and accurate approach for qPCR analysis
Performance evaluation considering the presence of a standard curve. A Coefficient of variation %: level-specific standard deviation as a percentage of the level-specific average. B Variance: within-level variance of the log10(predicted concentration). C Absolute relative error: the absolute value of the deviation of the predicted concentrations from the true concentrations. D Absolute bias: the absolute value of the ratio of the average of the highest to the lowest predicted concentrations divided by the ratio of the highest to the lowest true concentrations. In all indicators, lower is better. *p value < 0.05, **p value < 0.01, ***p value < 0.001, and ****p value < 0.0001