Fig. 5
From: Introducing the f0% method: a reliable and accurate approach for qPCR analysis
Effect of the position of the inflection cycle (Ci) on the coefficient of variation (%). Reactions of the first levels of the 20 dilution curves were used to calculate f0% 11 times. Each time the reaction was trimmed at a different cycle relative to the Ci so that the final cycle (Cfinal) ranged from (Cfinal = Ci − 5) to (Cfinal = Ci + 5). On the left side, amplification curves with different Ci ranging from (Cfinal = Ci − 5) to (Cfinal = Ci + 5). On the right side, a horizontal box plot representing the coefficient of variations (%) of the 20 dilution curves grouped by Ci