Fig. 5

Iterative clustering result. 720 protein signals were individually transformed to z scores and subsequently clustered by k means clustering (k = 5). A: Five clusters are depicted with cluster mean and 95% prediction interval (PI). Signal colours indicate whether 0 (grey), 1 (green), 2 (yellow), or more (orange) points of a signal lie outside of the PI. The table below shows the percentage of each group. B: The cluster mean intensity is visualized together with signals that showed the highest (green) and lowest (red) coefficient of determination (R²) to the cluster mean. C: The cluster mean intensity is plotted against the intensities of the signal of highest (green) and lowest (red) R² within the cluster. R² of both signals is given in each panel. D: Histogram of all R² values between signals and cluster mean. The percentage in each panel depicts the percentage of signals whose R² is lower than 0.8