Fig. 4

Costless secretion analysis. A Number of iterations required to achieve a stable media, for the aerobic (left) and anaerobic (right) states for optimization of biomass or different cutoffs for flux sampling (all, most, any), plotted as a percentage of all simulations. B Number of metabolites secreted for optimization of biomass or with distinct cutoffs for flux sampling (all, most, any) for anaerobic and aerobic conditions. C Each simulation was categorized into one of 7 cases (the six shown in the left panel and the case where no growth was achieved) for the aerobic or anaerobic condition