Fig. 4

Dashing Growth Curves sample view. ① Position of the currently selected sample and its name. The dropdown menu or the arrow buttons can be used to change the currently displayed sample. If there are 6, 12, 24, 96 or 384 samples, a sample position will be associated with each sample (e.g. the 13th row in a data file of 96 samples will be position B2) if there are a number of samples that are not associated with a standard microtiter plate size, each will be associated with a number. ② The sample name can be changed here. ③ The field displays the blanks associated with the current sample. The associated blanks can be changed by providing a list of sample names that are to be used as blanks. By default, Dashing Growth Curves takes the first three samples in a data file as blanks. The default blanks for all samples can be changed in ④. ⑤ Circle button: data can be smoothed using a rolling average algorithm (the default window is 10 data points). CPU button: Fit all growth curves to a chosen parametric growth model or with the Easy Linear algorithm (the option can be selected through the dropdown menu). The button to the right of the dropdown menu leads to the documentation of the different automatic growth parameter extraction approaches. ⑥ Graphs of the associated blanks and their mean (here all three blanks are very similar and fall onto one line), the raw trace of the currently selected sample and the blanked trace. ⑦ The blanked trace plotted on a logarithmic y-axis. Pressing the 'x' button excludes the sample from the summary view (Fig. 4). ⑧ Growth parameters of the currently selected sample. If the logarithmic growth phase has not been determined manually or the sample has not been fitted to a parametric growth model yet, the values are ‘nan’ (not a number)