Fig. 10

The RGB synthetic morphogenesis process for the second case study. (A) Cells start as a disorganized aggregate; (B) CD19 in Cells A activates anti-CD19 synNotch in Cells B, inducing the expression of a high level of E-cadherin (\(Ecad_{hi}\)) and \(GFP_{lig}\); (C) Cells B aggregate and form a compact group in the middle of the aggregate; (D) The \(GFP_{lig}\) on Cells B activates the anti-GFP synNotch receptors on Cells A in direct contact with the central group, inducing \(Ecad_{lo}\) and the mCherry reporter, (E) making a spatially organized pattern of cells emerge in a synthetic morphogenetic pattern with three concentric layers: a green internal core (Cells B expressing \(Ecad_{hi}\) and \(GFP_{lig}\)) with high cell-cell adhesion, an outer layer of blue cells (Cells A expressing BFP), and a population of red cells in the middle layer (Cells A expressing \(Ecad_{lo}\) and mCherry).Adapted from [57]