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Fig. 9 | BMC Bioinformatics

Fig. 9

From: Biology System Description Language (BiSDL): a modeling language for the design of multicellular synthetic biological systems

Fig. 9

The multicellular synthetic circuit mediated by cell-cell communication makes the RGB pattern emerge. (A) The Sender cells (Cells A) constitutively express blue fluorescent protein (BFP), CD19 ligand, and the anti-GFP synthetic Notch (synNotch) receptor, which drives expression of a low amount of E-cadherin (\(Ecad_{lo}\)) fused with a (D) mCherry reporter for visualization. (B) Receiver cells (Cells B) inducibly express E-cadherin (\(Ecad_{hi}\)) and a modified form of GFP working as a synNotch ligand on the cell membrane (\(GFP_{lig}\)). (C) \(GFP_{lig}\) serves as both a fluorescent reporter and a ligand for a secondary synNotch receptor with the cognate anti-GFP binding domain. (E) Cells A have low adherence, blue fluorescence, and inducible red fluorescence; (F) Cells B have inducible green fluorescence and high adherence. Adapted from [57]

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BMC Bioinformatics

ISSN: 1471-2105

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