Manual assessment and validation in Hydra. The 'Manual assessment and validation' tool allows inspection and adjustment of individual experiments. Shown here is the replicate viewing mode, selected in the top left. XIC's and MS spectra from replicates are vertically aligned, to aid detection of anomalies. In this example, a particular peptide is selected from the project tree on the left. The XICs (left-most graphs) are very similar, while the second MS spectrum is different from the others. The user can then adjust the XIC selection (peak selection, XIC integration width or offset) using the controls in the right-most panel. The file will automatically reprocess as defined in the workflow. The right-most area also contains controls for displaying deuterium distributions, and extracting raw values (see lower-right hand portion of the figure).