Analysis by 1-way ANOVA (p < 0.005, Benjamin-Hochberg Multiple Test Correction (MTC) defined 311, 624, and 581 probesets (genes) with highly significant expression differences between strains at 2, 3 and 4 weeks, respectively. Hierarchical clustering of these probeset lists identified 58, 115, and 65 probesets, respectively for 2, 3, and 4 week old mice, as being differentially expressed in NOD mice relative to both control strains (17, 32, and 9 were upregulated in NOD mice while 41, 83, and 56 were downregulated). Pathway analysis of the 3 gene lists (58, 115 and 64 probesets) revealed that 7 (HNF4A, IFNG, IL4, TP53, BCL2L1, IL15, and prostaglandin E2) of the top 15 central genes in each network were common to all age groups. IL6 was central in networks of 2 and 4 week old mice but not of 3 week old mice. Uniquely central to individual networks were: MYC, JUN and HRAS at 2 weeks; TNF, NFKB, p38MAPK, and TGFB1 at 3 weeks; and Interferon alpha, IL12 and STAT4 at 4 weeks.