- Poster presentation
- Open Access
Enabling proteomics-based identification of human cancer variations
© Zhang et al; licensee BioMed Central Ltd. 2010
- Published: 23 July 2010
- Mutation Information
- Protein Identification
- HCT116 Cell
- Colorectal Cancer Cell
- Colorectal Cancer Cell Line
Shotgun proteomics is a powerful technology for protein identification in complex samples with remarkable applications in elucidating cellular and subcellular proteomes [1, 2], and discovering disease biomarkers [3, 4]. Shotgun proteomics data analysis usually relies on database search. Commonly used protein sequence databases in shotgun proteomics data analysis do not contain mutation information. This becomes a problem in cancer studies in which the detection of disease-related mutated peptides/proteins is crucial for understanding cancer biology . Including protein mutation information into sequence databases can help alleviate this problem.
Owing to its protein-centric nature, the approach we proposed can serve as a bridge between genomic variation data and proteomics studies in human cancer.
This work was supported by the National Institutes of Health (NIH)/ National Cancer Institute (NCI) through grant R01 CA126218 and the NIH/National Institute of General Medical Sciences through grant R01 GM88822.
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