ZFN half-sites and resulting query sequences with 5 or 6 bp spacing. ZFN-Site generates six query sequences based on the two half-sites entered for homo-dimerization, and two different spacings are allowed between the half-sites. The left and right half-sites are listed followed by the resulting query sequences if the half-sites are separated by 5 or 6 bps. Each list includes a query string made of one left and one right half-site (L + R), two left half-sites (L + L) and two right half-sites (R + R). Each of these would need to be searched individually if using BLAST.