Volume 13 Supplement 12

UT-ORNL-KBRIN Bioinformatics Summit 2012

Open Access

Temporal and spatial relationship of gene expression in the infarcted rat heart

BMC Bioinformatics201213(Suppl 12):A16


Published: 31 July 2012


Gene interaction plays an important role in regulating the molecular and cellular actions in both the physiological and pathological circumstances. Following myocardial infarction (MI), cardiac repair/remodeling represented as cardiac inflammation, angiogenesis, apoptosis, fibrosis occur in the infarcted and noninfarcted myocardium. Local angiotensin system (RAS) is involved in the cardiac repair.

Materials and methods

In the current study, thirty-five genes involved in cardiac repair and RAS were detected by Real Time PCR in both the infarcted and non-infarcted myocardium at different time point post-MI. Pearson correlation analysis showed a number of significant correlations between the expressions of these genes. The different phases of cardiac repair/remodeling and regions of the infarcted heart impacted the correlation directions (positive or reverse), correlation efficient value, and corresponding significant levels. Multiple linear regression models fitting also showed that the different phases of cardiac repair/remodeling and different regions of the infarcted heart determined the dependent factors in the models. The interactions among genes were further explored by Ingenuity Pathway Analysis software. We found that several pathological pathways were involved in cardiac repair and remodeling in the different regions of the infarcted heart at different stages postMI.

Authors’ Affiliations

Division of Cardiovascular Diseases, Department of Medicine, University of Tennessee Health Science Center


© Zhao et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.