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BMC Bioinformatics

Open Access

CLIP-EZ: a computational tool for HITS-CLIP data analysis

BMC Bioinformatics201415(Suppl 10):P1

https://doi.org/10.1186/1471-2105-15-S10-P1

Published: 29 September 2014

Background

Mapping the binding regions of mRNA-binding proteins is critical to the understanding of their regulatory roles in cellular processes. Recent development in experimental technologies combines high throughput sequencing with crosslink immunoprecipitation (HITS-CLIP), which has the merit of detecting RNA-protein interaction sites at a high resolution to single nucleotide level. Analysis of such data typically involves many steps, and teasing out true signals from noise requires crosslink induced mutations (CIMS) analysis, peak identification, integration of the two signal types, and some downstream analysis such as motif finding and conservation evaluation. To our knowledge, there is a lack of a single computational tool that can perform all the tasks as mentioned in an easily accessible manner. Despite the fact that there are several tools available, each performs an individual task.

Materials and methods

To facilitate the analysis task, we developed a command line tool that implements the analysis pipeline and outputs all relevant results, intermediate and final, with command line parameters specified by the user.

Results

The outputs are comprehensive which include the map of binding regions, pie charts for the annotation of binding sites, a list of high confidence binding regions which are annotated for CIMS footprint, peak height, motif, and conservation score and ranked by a score that integrates information across the annotations. The output also provides a bed file that can be loaded into the UCSC genome browser for visualization. This software can also be used to identify differential binding regions across biological conditions. This command line tool will be made available for download from GitHub.

Authors’ Affiliations

(1)
Center for Quantitative Sciences, Vanderbilt University School of Medicine

Copyright

© Zhong et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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