Population substructure in Cache County, Utah: the Cache County study
© Sharp et al.; licensee BioMed Central Ltd. 2014
Published: 28 May 2014
Population stratification is a key concern for genetic association analyses. In addition, extreme homogeneity of ethnic origins of a population can make it difficult to interpret how genetic associations in that population may translate into other populations. Here we have evaluated the genetic substructure of samples from the Cache County study relative to the HapMap Reference populations and data from the Alzheimer's Disease Neuroimaging Initiative (ADNI).
Our findings show that the Cache County study is similar in ethnic diversity to the self-reported "Whites" in the ADNI sample and less homogenous than the HapMap CEU population.
We conclude that the Cache County study is genetically representative of the general European American population in the USA and is an appropriate population for conducting broadly applicable genetic studies.
Cryptic population differences due to heterogeneity have confounding effects on association studies, especially when analyzing complex traits and gene-gene interactions. It is also possible that genetic associations that are observed in very homogeneous populations may not be generalizable to other populations.
The Cache County study is a large longitudinal cohort study of memory, health, and aging that was initiated in 1994. This sample of 5,092 individuals represents approximately 90% of the Cache County population aged 65 and older at that time. These data are a valuable resource in genetic studies of Alzheimer's disease (AD), as well as otherdiscussion forms of dementia . The founding populations and migrations to Utah by early members of the Church of Jesus Christ of Latter Day Saints have been studied extensively[2–4]. These studies have concluded that due to the large founding population, high rates of gene flow and diversity of source populations the Utah population has allele frequencies that are quite similar to the general European American population in the United States. As it was collected from one county in Utah, there have been questions as to how the genetic diversity in the Cache County sample compares to that of more broadly collected European American samples and whether that diversity affects the validity or generalizability of results obtained from the Cache County study.
The Cache County study is a large longitudinal cohort study of memory, health, and aging that was initiated in 1994. This sample of 5,092 individuals represents approximately 90% of the Cache County population aged 65 and older at that time. These data are a valuable resource in genetic studies of Alzheimer's disease (AD), as well as other forms of dementia . The founding populations and migrations to Utah by early members of the Church of Jesus Christ of Latter Day Saints have been studied extensively[2–4]. These studies have concluded that due to the large founding population, high rates of gene flow and diversity of source populations the Utah population has allele frequencies that are quite similar to the general European American population in the United States. As it was collected from one county in Utah, there have been questions as to how the genetic diversity in the Cache County sample compares to that of more broadly collected European American samples and whether that diversity affects the validity or generalizability of results obtained from the Cache County study.
The purpose of this study is to compare the genetic structure of the Cache County study, which was collected from one county in northern Utah, to that of the Alzheimer's Disease Neuroimaging Initiative, a sample that was collected from several sites around the USA, and data from several other populations from the International HapMap Project.
Sample collection and genotyping
The Cache County study originally included 5,092 permanent residents of Cache County over the age of 64. Details of collection methods and demographics of the Cache County samples have been reported previously . Briefly, samples underwent four triennial waves of data collection in a multi-stage dementia screening and assessment protocol. DNA was obtained from blood and buccal swabs as described by Breitner et al.  All study procedures were approved by the Institutional Review Boards of Utah State, Duke and Johns Hopkins universitys. DNA from 506 Cache County study participants was genotyped using the Illumina OmniExpress chip; DNA from 234 others was genotyped using the Illumina 2.5M chip.
HapMap populations with Abbreviations
African ancestry in Southwest USA
CEPH (Utah residents with ancestry from northern and western Europe)
Han Chinese in Beijing, China
Chinese in Metropolitan Denver, Colorado
Gujarati Indians in Houston, Texas
Japanese in Tokyo, Japan
Luhya in Webuye, Kenya
Mexican ancestry in Los Angeles, California
Maasai in Kinyawa, Kenya
Tuscan in Italy
Yoruban in Ibadan, Nigeria
The ADNI samples are part of a longitudinal study designed to measure the progression of mild cognitive impairment (MCI) and early AD . The primary goal of ADNI has been to test whether serial magnetic resonance imaging (MRI), PET, other biological markers, and clinical and neuropsychological assessment can be combined to measure the progression of MCI and early AD (PI: Michael W. Weiner, M.D., VA Medical Center and University of California - San Francisco). ADNI is the result of efforts of many co- investigators from a broad range of academic institutions and private corporations, and subjects have been recruited from over 50 sites across the U.S. and Canada. For up-to-date information, see http://www.adni-info.org. Data for the present analysis were downloaded from the ADNI web site in March 2013. We used self-reported race to label three ethnic groups within the ADNI samples, Black/African-American (ADNI1), White (ADNI2), or Asian (ADNI3).
Data preparation and quality control
For the distance-based clustering, the following data preparation steps were taken. First, all palindromic markers (alleles A/T or G/C) were excluded to correct for strand differences between datasets (120,541 markers excluded). We then excluded all markers that were not genotyped in at least two of the three data sets (342,603 markers included). Finally, we removed all markers with genotyping rates lower than 95% across all individuals (263,883 markers included). Individuals with genotyping rates of lower than 95% for the included markers were excluded from the analysis. The final set consisted of 2,274 individuals, 400 from Cache, 626 from ADNI, and 1,248 from the various HapMap populations.
For the model-based clustering, we compared Cache to only two populations (CEU and ADNI2), because they appeared most similar in the distance-based method. This included a total of 1,155 samples. Pruning of markers to minimize redundant information due to linkage disequilibrium was done using the following parameters in PLINK: r2 > .10 in 50 SNP windows, incremented by 5 SNPs across the genome (61616 markers included).
Methods for distance and model-based clustering analysis
The distance-based method of comparison is a standard classical (metric) multi-dimensional scalar analysis of pairwise identity by state distances. Distances were calculated and scaling was performed using the PLINK whole genome association analysis toolset .
In addition to distance scaling, Bayesian modeling techniques were leveraged to identify clustering of samples from Cache and the other European-American populations. The model-based clustering method was provided using the program Structure . Burn-in duration was set at 5,000 repetitions with a run duration of 10,000 repetitions per software recommendations. We used an admixture type ancestry model due to the generally admixed nature of the European American population and specified for a model of correlated allele frequency across result populations. The statistic of interest generated by Structure is a probability, Pr (Z | P, X), where Z is a vector containing the assignment to a cluster of each individual, P specifies the frequency of each allele at each locus (a statistic that is calculated by Structure to characterize each cluster), and × is the given genotypes of the sampled individuals.
Results from the distance-based clustering approach
Results from the model-based clustering approach
Log of probability of (k) from the combined ADNI2, CEU and Cache County Samples calculated using Structure.
Prior population estimate (k)
ln( P ( Z | P, X) )
Cluster assignments from structure.
Proportion of individuals assigned to minor result cluster
Proportion of individuals assigned to major result cluster
The Cache County study has provided important information about genetic factors that influence aging and Alzheimer's disease [1, 9–13]. The results reported here indicate that despite being collected from a single county in northern Utah, these samples are comparable in levels of diversity to those of self-reported European-Americans collected at multiple sites across the United States. In addition, we observed no evidence of problematic population stratification between the Alzheimer's disease cases and non-demented controls in the Cache samples. This indicates that association studies in this population are unlikely to produce type-I error due to population stratification.
The distance-based clustering method identified similarity between the majority of European-American individuals, including those from Cache County, CEU, and ADNI2. The model-base clustering method found the optimum solution to be two clusters, one small cluster with a small number of outliers from each of the three samples (Cache, ADNI2, and CEU) and a larger cluster that included the vast majority of samples.
These data suggest that the population substructure in the Cache County study is comparable to that of other European-American samples that are more broadly collected, (i.e. the ADNI samples). In addition, these findings are consistent with the results reported previously on the larger population of the early population of the state of Utah [2–4]. We conclude that despite concerns about the limited geographic range of sample collection in the Cache County study, the results of genetic studies in this population, such as gene discovery, validation, and estimates of population level effects, are as broadly applicable to other populations of European American ancestry as that of the Alzheimer's Disease Neuroimaging Initiative.
The authors gratefully acknowledge the individuals who participated in this study without whom this work would not have been possible. We also acknowledge Alzheimer's Disease Neuroimaging Initiative (ADNI) for making their data publically available for this and other work(http://www.loni.ucla.edu/ADNI), as such, the investigators within the ADNI contributed to the design and implementation of ADNI and/or provided data but did not participate in analysis or writing of this report. A complete listing of ADNI investigators can be found at: http://loni.ucla.edu//ADNI//Collaboration//ADNI_Authorship_list.pdf. Data collection and sharing for this project was funded by the ADNI (National Institutes of Health Grant U01 AG024904). ADNI is funded by the National Institute on Aging, the National Institute of Biomedical Imaging and Bioengineering, and through generous contributions from the following: Abbott; Alzheimer's Association; Alzheimer's Drug Discovery Foundation; Amorfix Life Sciences Ltd.; AstraZeneca; Bayer HealthCare; BioClinica, Inc.; Biogen Idec Inc.; Bristol-Myers Squibb Company; Eisai Inc.; Elan Pharmaceuticals Inc.; Eli Lilly and Company; F. Hoffmann-La Roche Ltd and its affiliated company Genentech, Inc.; GE Healthcare; Innogenetics, N.V.; Janssen Alzheimer Immunotherapy Research & Development, LLC.; Johnson & Johnson Pharmaceutical Research & Development LLC.; Medpace, Inc.; Merck & Co., Inc.; Meso Scale Diagnostics, LLC.; Novartis Pharmaceuticals Corporation; Pfizer Inc.; Servier; Synarc Inc.; and Takeda Pharmaceutical Company. The Canadian Institutes of Health Research is providing funds to support ADNI clinical sites in Canada. Private sector contributions are facilitated by the Foundation for the National Institutes of Health (http://www.fnih.org). The grantee organization is the Northern California Institute for Research and Education, and the study is coordinated by the Alzheimer's Disease Cooperative Study at the University of California, San Diego. ADNI data are disseminated by the Laboratory for Neuro Imaging at the Rev August 16 2011 University of California, Los Angeles. This research was also supported by NIH grants P30 AG010129, K01 AG030514, R01 HG02213, U01 AG024904, U01 HG006500, K24 AG027841, R01 HG005092, P50AG005146, and the Dana Foundation.
This work was supported by the Alzheimer's Association (MNIRG-11-205368), the BYU Gerontology Program and the National Institutes of Health (R01-AG11380, R01- AG021136, P30-NS069329-01, R01-AG042611). The Institutional Review Boards of each appropriate institution approved sample and data collection.
This article has been published as part of BMC Bioinformatics Volume 15 Supplement 7, 2014: Selected articles from the 10th Annual Biotechnology and Bioinformatics Symposium (BIOT 2013). The full contents of the supplement are available online at http://www.biomedcentral.com/bmcbioinformatics/supplements/15/S7
- Breitner JC, Wyse BW, Anthony JC, Welsh-Bohmer KA, Steffens DC, Norton MC, Tschanz JT, Plassman BL, Meyer MR, Skoog I: APOE-epsilon4 count predicts age when prevalence of AD increases, then declines: the Cache County Study. Neurology. 1999, 53: (2):321-331.View ArticlePubMedGoogle Scholar
- O'Brien E, Rogers AR, Beesley J, Jorde LB: Genetic structure of the Utah Mormons: comparison of results based on RFLPs, blood groups, migration matrices, isonymy, and pedigrees. Hum Biol. 1994, 66: (5):743-759.PubMedGoogle Scholar
- McLellan T, Jorde LB, Skolnick MH: Genetic distances between the Utah Mormons and related populations. Am J Hum Genet. 1984, 36: (4):836-857.PubMed CentralPubMedGoogle Scholar
- Jorde LB: The genetic structure of the Utah Mormons: migration analysis. Hum Biol. 1982, 54: (3):583-597.PubMedGoogle Scholar
- International HapMap C: The International HapMap Project. Nature. 2003, 426: (6968):789-796.Google Scholar
- Petersen RC, Aisen PS, Beckett LA, Donohue MC, Gamst AC, Harvey DJ, Jack CR, Jagust WJ, Shaw LM, Toga AW: Alzheimer's Disease Neuroimaging Initiative (ADNI): clinical characterization. Neurology. 2010, 74: (3):201-209.PubMed CentralPubMedGoogle Scholar
- Purcell S, Neale B, Todd-Brown K, Thomas L, Ferreira MA, Bender D, Maller J, Sklar P, de Bakker PI, Daly MJ: PLINK: a tool set for whole-genome association and population-based linkage analyses. Am J Hum Genet. 2007, 81: (3):559-575.PubMed CentralView ArticlePubMedGoogle Scholar
- Falush D, Stephens M, Pritchard JK: Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics. 2003, 164: (4):1567-1587.PubMed CentralPubMedGoogle Scholar
- Guerreiro R, Wojtas A, Bras J, Carrasquillo M, Rogaeva E, Majounie E, Cruchaga C, Sassi C, Kauwe JS, Younkin S: TREM2 variants in Alzheimer's disease. The New England journal of medicine. 2013, 368: (2):117-127.PubMed CentralView ArticlePubMedGoogle Scholar
- Peterson D, Munger C, Crowley J, Corcoran C, Cruchaga C, Goate AM, Norton MC, Green RC, Munger RG, Breitner JC: Variants in PPP3R1 and MAPT are associated with more rapid functional decline in Alzheimer's disease: The Cache County Dementia Progression Study. Alzheimers Dement. 2013Google Scholar
- Ridge PG, Maxwell TJ, Corcoran CD, Norton MC, Tschanz JT, O'Brien E, Kerber RA, Cawthon RM, Munger RG, Kauwe JS: Mitochondrial Genomic Analysis of Late Onset Alzheimer's Disease Reveals Protective Haplogroups H6A1A/H6A1B: The Cache County Study on Memory in Aging. PLoS ONE. 2012, 7: (9):e45134-PubMed CentralView ArticlePubMedGoogle Scholar
- Gonzalez J, Schmutz C, Munger C, Perkes A, Gustin A, Peterson M, MTW E, Norton MC, Tschanz JT, Munger RG: Assessment of TREM2 R47H association with Alzheimer's disease in a population-based sample: The Cache County Study. Neurobiology of Aging. 2013Google Scholar
- Ebbert MT, Ridge PG, Wilson AR, Sharp AR, Bailey M, Norton MC, Tschanz JT, Munger RG, Corcoran CD, Kauwe JS: Population-based Analysis of Alzheimer's Disease Risk Alleles Implicates Genetic Interactions. Biological psychiatry. 2013Google Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.