Volume 16 Supplement 2

Highlights from the Tenth International Society for Computational Biology (ISCB) Student Council Symposium 2014

Open Access

The road to linking genomics and proteomics of pathogenic bacteria: from binary protein complexes to interaction pathways

  • Sarah L Keasey1,
  • Mohan Natesan1,
  • Christine Pugh1,
  • Teddy Kamata1,
  • Stefan Wuchty2 and
  • Robert G Ulrich1
BMC Bioinformatics201516(Suppl 2):A9


Published: 28 January 2015


The availability of fully sequenced genomes of many bacterial organisms has enabled mapping networks of binary protein interactions that form the basic building blocks of molecular pathways and dynamic assemblies defining all cellular activities. Few proteome-scale studies have been reported for pathogenic bacteria though, suggesting that a systems-wide network analysis of binary interaction partners could reveal groups of proteins that coordinate to achieve specific biological tasks important to pathogenesis and provide a functional map useful to the discovery of new antibiotics, vaccines, and diagnostic tools.


We performed a comprehensive proteomics analysis of the pathogenic bacterium Yersinia pestis and analytically identified more than 74,000 binary interactions. Using a library of biotinylated recombinant proteins to probe a planar microarray comprised of immobilized proteins that represented approximately 85% (3,552 proteins) of the Y. pestis proteome, we measured protein-protein interactions by fluorescence intensity of the laser-scanned microarrays. We obtained kinetic interaction data for >1,600 binary complexes by microarray-based, surface plasmon resonance imaging, and identified several high-affinity (K D ~ nM) interactions. We applied a machine learning algorithm that used previously reported experimental protein-protein interactions from Escherichia coli as a training set in order to extract E. coli-like interactions from the Y. pestis dataset. The node degree distribution of the resulting network, comprised of 2344 interactions between 314 proteins, approximates a power-law distribution typical of scale-free networks. Functional annotation clustering of proteins within the network revealed statistically enriched complexes and pathways involved in diverse biological processes. Among the more notable protein assemblies identified were components of the RNA polymerase enzyme and ribosomes. Small modules of proteins related to various metabolic pathways, as well as previously reported interactions involved in homologous recombination and fatty acid biosynthesis, were also present in the network. Two highly interconnected network sub-regions contained a large percentage of proteins with functions linked to transcription and translation.


We have systematically identified and analyzed thousands of direct binary protein interactions within Y. pestis. This new benchmark data set will serve as a critical tool for the analysis of protein interaction networks functioning within an important human pathogen.

Authors’ Affiliations

Molecular and Translational Sciences Division, U.S. Army Medical Research Institute of Infectious Diseases
National Center for Biotechnology Information, National Institutes of Health


© Keasey et al; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.