- Open Access
Identifying spatially similar gene expression patterns in early stage fruit fly embryo images: binary feature versus invariant moment digital representations
© Gurunathan et al; licensee BioMed Central Ltd. 2004
- Received: 30 April 2004
- Accepted: 16 December 2004
- Published: 16 December 2004
Modern developmental biology relies heavily on the analysis of embryonic gene expression patterns. Investigators manually inspect hundreds or thousands of expression patterns to identify those that are spatially similar and to ultimately infer potential gene interactions. However, the rapid accumulation of gene expression pattern data over the last two decades, facilitated by high-throughput techniques, has produced a need for the development of efficient approaches for direct comparison of images, rather than their textual descriptions, to identify spatially similar expression patterns.
The effectiveness of the Binary Feature Vector (BFV) and Invariant Moment Vector (IMV) based digital representations of the gene expression patterns in finding biologically meaningful patterns was compared for a small (226 images) and a large (1819 images) dataset. For each dataset, an ordered list of images, with respect to a query image, was generated to identify overlapping and similar gene expression patterns, in a manner comparable to what a developmental biologist might do. The results showed that the BFV representation consistently outperforms the IMV representation in finding biologically meaningful matches when spatial overlap of the gene expression pattern and the genes involved are considered. Furthermore, we explored the value of conducting image-content based searches in a dataset where individual expression components (or domains) of multi-domain expression patterns were also included separately. We found that this technique improves performance of both IMV and BFV based searches.
We conclude that the BFV representation consistently produces a more extensive and better list of biologically useful patterns than the IMV representation. The high quality of results obtained scales well as the search database becomes larger, which encourages efforts to build automated image query and retrieval systems for spatial gene expression patterns.
- Gene Expression Pattern
- Query Image
- Optical Character Recognition
- Zernike Moment
- Invariant Moment
The complexity of animal body form arises from a single fertilized egg cell in an odyssey of gene expression and regulation that controls the multiplication and differentiation of cells [1–3]. For over two decades, Drosophila melanogaster (the fruit fly) has been a canonical model animal for understanding this developmental process in the laboratory. The raw data from experiments consist of photographs (two dimensional images) of the Drosophila embryo showing a particular gene expression pattern revealed by a gene-specific probe in wildtype and mutant backgrounds. Manual, visual comparison of these spatial gene expressions is usually carried out to identify overlaps in gene expression and to infer interactions [4–6].
Whole fruit fly embryo and other related gene expression patterns have been published in a wide variety of research journals since late 1980's. These efforts have now entered a high-throughput phase with the systematic determination of patterns of gene expression [e.g., ]. As a result, the amount of data currently available has doubled leading to the imminent availability of multiple expression patterns of every gene in the Drosophila genome . In addition, the use of micro-array technology to study Drosophila development has revealed additional and important insights into changes in gene expression levels over time and under different conditions at a genomic scale [8, 9].
With this rapid increase in the amount of available primary gene expression images, searchable textual descriptions of images have become available [7, 10, 11]. However, a direct comparison of the gene expression patterns depicted in the images is also desirable to find biologically similar expression patterns, because textual descriptions (even using a highly structured and controlled vocabulary) cannot fully capture all aspects of an expression pattern. In fact, there is a need for automated identification of images containing overlapping or similar gene expression patterns [6, 12] in order to assist researchers in the evaluation of similarity between a given expression pattern and all other existing (comparable) patterns in the same way that the BLAST  technique functions for DNA and protein sequences. Of course, unlike the genomes with four letters and proteomes with 20 letters, all gene expression anatomies cannot be easily reduced to, and thus represented by, a small number of components.
We previously proposed a binary coded bit stream pattern to represent gene expression pattern images . In this digital representation, referred to as the Binary Feature Vector (BFV; BSV in ), the unstained pixels in the images (white regions and background) were denoted by a value of 0 and the stained areas (colored and foreground: gene expression) were denoted by a value of 1. Based on the BFV representations of the expression pattern, we proposed a Basic Expression Search Tool for Images (BESTi)  with an aim to produce biologically significant gene expression pattern matches using image content alone, without any reference to textual descriptions. We found that the BESTi approach generated biologically meaningful matches to query expression patterns .
In this paper, we explore how a more sophisticated Invariant Moment Vectors (IMV, ) based digital representation of gene expression patterns performs in generating an ordered list of best-matching images that contain similar/overlapping gene expression patterns to that depicted in a query image. IMV are frequently used in natural image processing (e.g., optical character recognition ) and have a number of desirable properties, including the compensation for variations of scale, translation, and rotation. If successful, IMV representations hold the promise of producing significantly shorter computing times for image-to-image matching compared to BFV.
Previously, we had examined the performance of the BFV representation for a limited dataset of early stage images . Here we compare the relative performances of BFV and IMV first using a dataset containing 226 images (from 13 research papers). Then we test for scalability of the BESTi search by using a seven times larger dataset containing 1819 (1593 new + 226 previous) images from 262 additional research papers (list available upon request from the authors). Both datasets contained lateral views of early stage (1–8) embryos.
During these investigations, we also developed another measure of image-to-image similarity for the BFV representation. This measure is aimed at finding images that contain as much of the query image expression pattern as possible, but without penalizing for the presence of any expression outside the overlap region in the target image. In addition, we examined whether partitioning a multi-domain expression pattern into multiple BFV representations, each containing only one domain, yields a better result set.
Recently, Peng and Myers  have proposed a different procedure involving the global and local Gaussian Mixture Model (GMM) of the pixel intensities (of expression) to identify images with similar patterns. This GMM method is expected to find images with intensity and spatial similarities. This is different from the BFV and IMV methods examined here, which are intended to find only spatially similar patterns. This focus is important because, as mentioned in , the differences in gene expression intensity among images in published literature can arise simply due to use of different techniques, illumination conditions, or biological reasons. However, Peng and Myers method  appears to be promising and we plan to examine its effectiveness in a separate paper.
Data set generation
An image database of 226 gene expression pattern images was initially generated using data from the literature [17–29]. All were lateral images and exhibited early stage (1–8) expression patterns. These images were selected because they had some commonality of gene expression (as seen by the human eye), which allowed us to evaluate the performance of the BESTi in finding correct as well as false matches under controlled conditions. BESTi was also tested for scalability on a larger dataset containing 1819 (1593 plus the 226) lateral views of early stage embryos. These 1593 images were obtained from 262 articles.
In order to present comprehensible result sets in this paper, we have primarily discussed the findings from the dataset of 226 and provided information on how those queries scaled when they were conducted for the larger dataset. In general, our focus was to show the retrieval of biologically significant matches based on both the visual overlap of the spatial gene expression pattern and the genes associated with the pattern retrieved.
Each image was standardized and the binary expression pattern extracted following the procedures described previously . These extracted patterns, their invariant moments (φ 1 through φ 7 ), and binary feature representations were stored in a database. We also calculated and stored the expression area (the count of the number of 1's in the binary feature represented image), the X and Y coordinates of the centroid ( , ), and the principal angle (θ) for each extracted pattern.
To quantify the similarity of gene expressions in two images, we computed two measures (SS, SC) based on the BFV representation (See equations 2 and 3 in Methods). S S is designed to find gene expression patterns with overall similarity to the query image, whereas S C is for finding images that contain as much of the query image expression pattern as possible without penalizing for the presence of any expression outside the overlap region in the target image. For a given pair of gene expression patterns (A and B), S S is the same irrespective of which image in the pair is the query image. That is, S S (A,B) = S S (B,A). This is not so for S C , because S C measures how much of the query gene expression pattern is contained in the image. Therefore, S C (A,B) ≠ S C (B,A).
For IMV representation, we computed one dissimilarity measure (D φ , equation 13 in Methods). Results from D φ should be compared to that from SS, as both of these measurements do not depend on the reference image, i.e., D φ (A,B) = D φ (B,A) and, also they capture overall similarity or dissimilarity.
Matches and their biological significance
The effectiveness of the BESTi in finding biologically similar expression patterns was geared towards determining the biological validity of the results obtained from the image matching procedure. All results were based solely on quantitative similarities between images without using any textual descriptions. All images were lateral views from the early stages of fruit fly embryogenesis and were oriented anterior end to the left and dorsal to the top. We refer to the images retrieved as the BESTi-matches.
Performance of BFV-S S search
Performance of IMV search
We used the same query image for the IMV method applied to the smaller dataset (D φ , results in Figure 1B) and compared the results to the BFV-S S search. In this case, we obtain images containing expressions of hb, Kr, tailless (tll), slp1, hairy and infra-abdominal (iab) (type I transcript). It is clear that IMV search produces some biologically disconnected matches. For example, Figures 1B2, 1B4–B7 exhibit no visual overlap in gene expression pattern with the query. Furthermore, even the biologically significant matches were retrieved out of order (Figure 1B1 before 1B3). This happens because D φ retrieves expression patterns that are of similar shape and/or size, regardless of the translation or rotation with respect to the query image.
Since both S S and D φ measures capture the overall similarity or dissimilarity, we can use Figures 2 and 3 to compare the relative effectiveness of the BFV and IMV methods on the larger dataset. We clearly see that the BFV method performs much better in retrieving both overlapping and similar expression patterns that are also biologically significant.
In addition to the Hu moments, one could also compute Zernike moments, which are based on the polar coordinate system. Both Hu moments and Zernike moments are susceptible to the same problem namely expression patterns showing a similar shape but translated to different locations in the embryo would be in the same result set. We chose to study the Hu Invariant Moment Vectors mainly because the centroid of the image can be used to distinguish between similarly shaped but translated expression patterns. With Zernike moments, the image must be inherently contained within a unit circle anchored at the centroid . Thus, there is no straightforward method to eliminate the translational problem.
Using the Hu moments, the spatial location problem can be corrected by considering the Euclidean difference in the centroid location expressed in pixels (ΔC XY ) of the query and results. In the case of BFV-S S search results in Figure 1 (A1–A8), the maximum ΔC XY is less than or only slightly greater than the minimum ΔC XY for the IMV search results (Figure 1 B1–B8). Therefore, in the present case, the IMV-based BESTi search results need to be pared down using the centroid location difference. For example, if we consider results based on a ΔC XY lesser than or equal to 50 pixels, images shown in Figure 1 B2, B4–B7 would be removed producing a more meaningful result set.
Performance of BFV-S C search
Figure 1C shows the result for the same query image as used in Figure 1A, but using the newly devised S C distance for the BFV representation (BFV-S C search). This is expected to retrieve images with gene expression patterns that contain the largest amount of the overlap with the expression pattern in the query image. The top eight hits shown (Figure 1C1–C8) all contain over 93% of the query expression pattern: five of the matches are to the expression of hunchback (hb; C1, C3–C6) and the remaining three are from slp1 under different genetic backgrounds. As mentioned above, the combinatorial input from gap genes (including hb) along with slp1 establishes the domains of segment polarity genes in the head . Therefore, gene expression patterns found by BFV-S C search are for developmentally connected genes. However, using the same query image, BFV-S C search yielded only two images in common with the BFV-S S results (Figure 1; C7 and C8 are the same as A5 and A4, respectively). This difference occurs because S S is designed to find gene expression patterns with overall similarity to the query image (Figure 1A), whereas S C is intended for finding images that contain as much of the query image expression pattern as possible and exclusive of the presence of the gene expression in the result image outside the region of overlap with the query image. Therefore, BFV-S S and BFV-S C have the capability of finding gene expression patterns from different biological perspectives.
Analysis of multi-domain gene expression patterns
Due to the presence of multiple areas of expression, some patterns in the database that appeared to contain much better matches (by eye and biologically) to the query image were not found or ranked very high. Hence, we also analyzed multi-domain expression patterns separately for the smaller dataset. Developmental biologists are also interested in finding such patterns as they contain overlaps with the expression domains in the query image. In fact, a large number of the expression patterns available today contain multiple isolated domains of expressions since more than one topologically distinct region of expression may be produced by many genes, transgenic constructs, probes or experimental techniques (multiple staining). In such cases, we need to consider each of these regions individually as well as in the context of the composite pattern. Biologically, it is important to consider them separately because different regions of expression may be under the control of distinct cis-regulatory sequences [e.g., [28, 38]] or may represent the expression of different genes in a multiply-stained embryo.
Separating multi-domain gene expression patterns into individual components was straightforward; we simply generated multiple images from the same initial image and included them in the target dataset. This resulted in 192 additional images (418 total) in the database all of which were components of the initial gene expression patterns. The images were separated into expression regions horizontally and/or vertically depending on the gene expression. For this new set of images, the IMV as well as BFV representations were re-calculated and the BESTi query constructed as above.
Results from BFV-S S and IMV queries for this data set are given in Figures 1D and 1E, respectively. Now, many images with multiple regions of expression are retrieved in the result set (Figure 1D: D1–D8) and many of them show an even better match with the query pattern than those in Figure 1A for the BFV-based BESTi search. For instance, gene expression patterns are now retrieved (with more than 55% pattern similarity) from embryos with the expression of tailless (tll), which is known to interact with slp1 in defining the embryonic head , and with a composite expression of race (related to angiotensin converting enzyme), sog (short gastrulation) and eve (even-skipped) due to enhanced race expression in the anterior domain caused by a transgenic construct causing ectopic expression of sog . Therefore, the strategy of dividing multi-domain expression data into individual domains provides additional flexibility to query individual components or sub-sets of complex expression patterns. Results also improved for IMV (Figure 1E), but again the outcome reinforced the need to use the difference in centroid to limit the result set.
When D φ is used as a search criterion, it produces some correct matches in the result set (Figure 5B1–B8). However, it generally fails to rank biologically meaningful matches as the best matches. Use of the centroid in this case is also not productive, as most of the matches show very close centroids. The principal angle (θ) value calculated does not show a significant difference in the early stage embryos used in this study. The results using the S C based search are given in Figure 5C1–C8. They show a number of images in common with the S S results. However, as expected, there are significant differences between the two searches.
The results in Figures 5D and 5E demonstrate the power of the BESTi-search when the multi-domain expression data are represented in their component patterns (domain database). In this case, all the BESTi searches are based on the use of S S as the search criterion. These searches are based on the complete expression (Figure 5D) and on one of its components (bottom-left domain, Figure 5E). All, but one, BESTi-matches in Figure 5D contain both domains of expression. In contrast, the use of only the left, anterior, domain (Figure 5E) in the BESTi search produces many other images in which the gene expression pattern is similar to only the anterior-ventral query pattern. Therefore, the use of individual expression components as search arguments increases the potential of directly identifying different overlapping expression patterns.
We have found that it is possible to identify biologically significant gene expression patterns from a dataset by first extracting numeric signature descriptors and then using those descriptors in a computerized search of the database for expression patterns with similar signatures or maximum pattern similarities. We find that the BFV methodologies provide a longer and more biologically meaningful set of expression pattern matches than IMV. Even though IMV representations will produce much faster retrieval speeds for large collections of embryogenesis images, the lack of biological validity of BESTi-matches retrieved makes IMV undesirable for the present problem. Instead, investigations and strategies aimed at improving the real time performance of the BFV representation will better serve the developmental biological research.
The wide variety of input methodologies, illumination conditions, equipment, and publication venues involved in the acquisition and presentation of gene expression patterns makes the available gene expression pattern data rather diverse. Extracting a gene expression pattern from its background requires the use of a combination of manual and automatic techniques. Each image is first standardized into a binary image as described in . The standardized images are then represented using the Binary Feature Vector (BFV) , and the Invariant Moment Vectors (IMV) . Similarity measures S S and S C are derived from BFV of which, S S is the one's complement of the distance metric D E presented in  and S C is a new measure introduced in this paper. The third metric D φ is deduced from the invariant moment vectors.
Binary Sequence Vector analysis
The binary coded bit stream pattern, in which the two possible states indicate staining over or under a threshold value, is called as Binary Feature Vector (BFV). This is referred to as the Binary Sequence Vector (BSV) in . In other words, we represent each image as a sequence of 1's and 0's, where the black pixels (stained areas) are denoted by a value of 1 and the white pixels (unstained and background) are denoted by a value of 0. This BFV holds the gene expression and localization pattern information of each image.
The expression patterns are ordered by evaluating a set of difference values, D E , between the binary feature vectors of every possible pair of images in the dataset. D E was introduced in  and is formally given as,
D E = Count(A XOR B)/Count(A OR B) (1)
The term Count(A XOR B) corresponds to the number of pixels not spatially common to the two images and the term Count(A OR B) provides the normalizing factor, as it refers to the total number of stained pixels (expression area) depicted in either of the two images being compared. For simplicity, we use the one's complement of D E , as a measure of similarity of gene expression patterns between two images, S S , is given by the equation
S S = (1 - D E ). (2)
S S quantifies the amount of similarity based on the overlap between two expression patterns. S S is equal to 1 when the two expression patterns are identical (D E = 0).
We introduce a new similarity measure in this paper that does not penalize for any non-overlapping region. The measure S C quantifies the amount of similarity based on the containment of one expression pattern in the other given by
S C = Count(A AND B)/Count (A) (3)
If the entire query image is contained within the result set images found in the database, i.e., there is complete overlap (with respect to the query image) S C is equal to 1. Note that, S C (A,B) ≠ S C (B,A), because the denominator corresponds to the gene expression area of the query image.
Invariant Moment Vector (IMV) analysis
Some methodologies of image analysis produce numeric descriptors that compensate for variations of scale, translation and rotation. In the following section, we describe the invariant moment analysis of gene expression data. Invariant moment calculations have been used in optical character recognition and other applications for many years .
To calculate these invariant moment descriptors the standardized binary image  is converted to a binary representation of the same pattern (BFV). From this binary sequence of the image, the invariant moments and other descriptors are extracted using the following method [14, 41]. The continuous scale equation used is
M pq = ∬x p y q f(x, y)dxdy, (4)
where M pq is the two-dimensional moment of the function of the gene expression pattern, f(x, y). The order of the moment is defined as (p + q), where both p and q are positive natural numbers. When implemented in a digital or discrete form this equation becomes
Discrete representations of the central moments are then defined as follows:
A further normalization for variations in scale can be implemented using the formula,
where φ 7 is a skew invariant to distinguish mirror images. In the above, φ 1 and φ 2 are second order moments and φ 3 through φ 7 are third order moments. φ 1 (the sum of the second order moments) may be thought of as the "spread" of the gene expression pattern; whereas the square root of φ 2 (the difference of the second order moments) may be interpreted as the "slenderness" of the pattern. Moments φ 3 through φ 7 do not have any direct physical meaning, but include the spatial frequencies and ranges of the image.
In order to provide a discriminator for image inversion (and rotation), sometimes called the "6", "9" problem, it has been suggested [14, 42] that the principal angle be used to determine "which way is up". This is extremely important in embryo images because gene expression at the anterior and posterior regions may simply appear to be mirror images of each other to the invariant moments, but biologically they are completely distinct. The principal axis of the gene expression pattern f(x, y) is the angular displacement of the minimum rotational inertia line that passes through the centroid ( , ) and is given as:
The slope of the principal axis is called the principal angle θ. It is calculated knowing that the moment of inertia of f around the line is a line through ( , ) with slope θ. We can find the θ value at which the momentum is minimum by differentiating this equation with respect to θ and setting the results equal to zero. This produces the following equation:
Using the condition |θ| < 45° one can distinguish the "6" from the "9" and rotationally similar gene expression patterns.
In invariant moment analysis, our initial method of image comparison calculates the Euclidean distance between the images using all moments (φ 1 through φ 7 ) and combinations of these moments. For example, if the first two invariant moments are used, then
and the distance D ij , between a pair of images i and j where i, j = 1, 2,...n is given by
This can be expanded to use all of the moment variables. Here, the Euclidean distance, D φ , between any two images is calculated as
where i and q designate images whose distance is being calculated and j designates the parameters used in the distance calculation and j = 1, 2, ..., 7. This assumes that all moments have the same dimensions or that they are dimensionless.
Using this method, it is possible to rank each of the images in order of their similarity based on, for example, the first two invariant moments that have clear-cut physical meanings. Expansion to include additional moments or parameters can be performed in a number of ways. It is possible to add additional parameters to the distance calculation making sure that each of the parameters has the same dimension. For example, φ1 has the dimension of distance squared, while φ2 has the dimension of the fourth power of distance, thus requiring the square root function to equalize dimensions for comparable distance calculation purposes. In general, the greater number of invariant moments used in the distance calculation, the more selective the ranking. We have also allowed for the use of the centroids and principal angle as a means of list limiting.
We thank Dr. Robert Wisotzkey for biological remarks, Dr. Dana Desonie for editorial comments and Dr. Stuart Newfeld for useful suggestions. This research was supported in part by research grants from National Institutes of Health (S.K.) and the Center for Evolutionary Functional Genomics (S.K.) at the Arizona State University.
- Carroll SB, Grenier JK, Weatherbee SD: From DNA to Diversity: Molecular Genetics and the Evolution of Animal Design. Massachusetts, MA, Blackwell Scientific; 2000.Google Scholar
- Davidson E: Genomic Regulatory Systems: Development and Evolution. New York, NY, Academic Press; 2000.Google Scholar
- Rougvie AE: Control of developmental timing in animals. Nat Rev Genet 2001, 2: 690–701. 10.1038/35088566View ArticlePubMedGoogle Scholar
- Gieseler K, Wilder E, Mariol MC, Buratovitch M, Berenger H, Graba Y, Pradel J: DWnt4 and wingless elicit similar cellular responses during imaginal development. Dev Biol 2001, 232: 339–350. 10.1006/dbio.2001.0184View ArticlePubMedGoogle Scholar
- Takaesu NT, Johnson AN, Sultani OH, Newfeld SJ: Combinatorial Signaling by an Unconventional Wg Pathway and the Dpp Pathway Requires Nejire (CBP/p300) to Regulate dpp Expression in Posterior Tracheal Branches. Dev Biol 2002, 247: 225–236. 10.1006/dbio.2002.0693View ArticlePubMedGoogle Scholar
- Kumar S, Jayaraman K, Panchanathan S, Gurunathan R, Marti-Subirana A, Newfeld SJ: BEST: A novel computational approach for comparing gene expression patterns from early stages of Drosophila melanogaster development. Genetics 2002, 162: 2037–2047.PubMed CentralPubMedGoogle Scholar
- Tomancak P, Beaton A, Weiszmann R, Kwan E, Shu S, Lewis SE, Richards S, Ashburner M, Hartenstein V, Celniker SE, Rubin GM: Systematic determination of patterns of gene expression during Drosophila embryogenesis. Genome Biol 2002, 3: research0088.1–88.14. 10.1186/gb-2002-3-12-research0088View ArticleGoogle Scholar
- Montalta-He H, Reichert H: Impressive expressions: developing a systematic database of gene-expression patterns in Drosophila embryogenesis. Genome Biol 2003, 4: 205. 10.1186/gb-2003-4-2-205PubMed CentralView ArticlePubMedGoogle Scholar
- Arbeitman MN, Furlong EE, Imam F, Johnson E, Null BH, Baker BS, Krasnow MA, Scott MP, Davis RW, White KP: Gene expression during the life cycle of Drosophila melanogaster. Science 2002, 297: 2270–2275. 10.1126/science.1072152View ArticlePubMedGoogle Scholar
- FlyBase: The FlyBase database of the Drosophila genome projects and community literature. Nucleic Acids Research 1999, 27: 85–88. 10.1093/nar/27.1.85View ArticleGoogle Scholar
- Janning W: FlyView, a Drosophila image database, and other Drosophila databases. Seminars in Cell and Developmental Biology 1997, 8: 469–475. 10.1006/scdb.1997.0172View ArticlePubMedGoogle Scholar
- Bard JBI: Introduction: Making and filling gene-expression developmental databases. Seminars in Cell and Developmental Biology 1997, 8: 455–458. 10.1006/scdb.1997.0170View ArticlePubMedGoogle Scholar
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. Journal of Molecular Biology 1990, 215: 403–410. 10.1006/jmbi.1990.9999View ArticlePubMedGoogle Scholar
- Hu MK: Visual pattern recognition by moment invariants. IRE Transactions of Information Theory 1962, 179–187.Google Scholar
- Castleman KR: Digital Image Processing. New Jersey, Prentice Hall; 1996.Google Scholar
- Peng H, Myers EW: Comparing in situ mRNA expression patterns of Drosphila embryos: ; San Diego, CA. ACM Journals; 2004.Google Scholar
- Arnosti DN, Gray S, Barolo S, Zhou J, Levine M: The gap protein knirps mediates both quenching and direct repression in the Drosophila embryo. Embo J 1996, 15: 3659–3666.PubMed CentralPubMedGoogle Scholar
- La Rosee-Borggreve A, Hader T, Wainwright D, Sauer F, Jackle H: hairy stripe 7 element mediates activation and repression in response to different domains and levels of Kruppel in the Drosophila embryo. Mech Dev 1999, 89: 133–140. 10.1016/S0925-4773(99)00219-1View ArticlePubMedGoogle Scholar
- Ashe HL, Levine M: Local inhibition and long-range enhancement of Dpp signal transduction by Sog. Nature 1999, 398: 427–431. 10.1038/18892View ArticlePubMedGoogle Scholar
- Casares F, Sanchez-Herrero E: Regulation of the infraabdominal regions of the bithorax complex of Drosophila by gap genes. Development 1995, 121: 1855–1866.PubMedGoogle Scholar
- Goldstein RE, Jimenez G, Cook O, Gur D, Paroush Z: Huckebein repressor activity in Drosophila terminal patterning is mediated by Groucho. Development 1999, 126: 3747–3755.PubMedGoogle Scholar
- Grossniklaus U, Cadigan KM, Gehring WJ: Three maternal coordinate systems cooperate in the patterning of the Drosophila head. Development 1994, 120: 3155–3171.PubMedGoogle Scholar
- Gutjahr T, Frei E, Noll M: Complex regulation of early paired expression: initial activation by gap genes and pattern modulation by pair-rule genes. Development 1993, 117: 609–623.PubMedGoogle Scholar
- Hartmann C, Taubert H, Jackle H, Pankratz MJ: A two-step mode of stripe formation in the Drosophila blastoderm requires interactions among primary pair rule genes. Mech Dev 1994, 45: 3–13. 10.1016/0925-4773(94)90049-3View ArticlePubMedGoogle Scholar
- Hulskamp M, Pfeifle C, Tautz D: A morphogenetic gradient of hunchback protein organizes the expression of the gap genes Kruppel and knirps in the early Drosophila embryo. Nature 1990, 346: 577–580. 10.1038/346577a0View ArticlePubMedGoogle Scholar
- Hulskamp M, Tautz D: Gap genes and gradients - the logic behind the gaps. BioEssays 1991, 13: 261–268.View ArticlePubMedGoogle Scholar
- Hulskamp M, Lukowitz W, Beermann A, Glaser G, Tautz D: Differential regulation of target genes by different alleles of the segmentation gene hunchback in Drosophila. Genetics 1994, 138: 125–134.PubMed CentralPubMedGoogle Scholar
- Gaul U, Jackle H: Role of gap genes in early Drosophila development. Adv Genet 1990, 27: 239–275.View ArticlePubMedGoogle Scholar
- Gaul U, Jackle H: Pole region-dependent repression of the Drosophila gap gene kruppel by maternal gene products. Cell 1987, 51: 549–555. 10.1016/0092-8674(87)90124-3View ArticlePubMedGoogle Scholar
- Royet J, Finkelstein R: Pattern formation in Drosophila head development: the role of the orthodenticle homeobox gene. Development 1995, 121: 3561–3572.PubMedGoogle Scholar
- Stathopoulos A, Levine M: Linear signaling in the Toll-Dorsal pathway of Drosophila: activated Pelle kinase specifies all threshold outputs of gene expression while the bHLH protein Twist specifies a subset. Development 2002, 129: 3411–3419.PubMedGoogle Scholar
- Brent AE, MacQueen A, Hazelrigg T: The Drosophila wispy gene is required for RNA localization and other microtubule-based events of meiosis and early embryogenesis. Genetics 2000, 154: 1649–1662.PubMed CentralPubMedGoogle Scholar
- Zhang H, Levine M: Groucho and dCtBP mediate separate pathways of transcriptional repression in the Drosophila embryo. Proc Natl Acad Sci U S A 1999, 96: 535–540. 10.1073/pnas.96.2.535PubMed CentralView ArticlePubMedGoogle Scholar
- Teh C, Chin R: On Image Analysis by the Methods of Moments. IEEE Transactions on Patterns Analysis and Machine Intelligence 1988, 10: 496–513. 10.1109/34.3913View ArticleGoogle Scholar
- Riechmann V, Irion U, Wilson R, Grosskortenhaus R, Leptin M: Control of cell fates and segmentation in the Drosophila mesoderm. Development 1997, 124: 2915–2922.PubMedGoogle Scholar
- Cadigan KM, Grossniklaus U, Gehring WJ: Localized expression of sloppy paired protein maintains the polarity of Drosophila parasegments. Genes Dev 1994, 8: 899–913.View ArticlePubMedGoogle Scholar
- Bhat KM, van Beers EH, Bhat P: Sloppy paired acts as the downstream target of wingless in the Drosophila CNS and interaction between sloppy paired and gooseberry inhibits sloppy paired during neurogenesis. Development 2000, 127: 655–665.PubMedGoogle Scholar
- Sanchez L, Thieffry D: A logical analysis of the Drosophila gap-gene system. J Theor Biol 2001, 211: 115–141. 10.1006/jtbi.2001.2335View ArticlePubMedGoogle Scholar
- Frasch M, Warrior R, Tugwood J, Levine M: Molecular analysis of even-skipped mutants in Drosophila development. Genes Dev 1988, 2: 1824–1838.View ArticlePubMedGoogle Scholar
- Abbott MK, Kaufman TC: The relationship between the functional complexity and the molecular organization of the Antennapedia locus of Drosophila melanogaster. Genetics 1986, 114: 919–942.PubMed CentralPubMedGoogle Scholar
- Jayaraman K, Panchanathan S, Kumar S: Classification and indexing of gene expression images. Proceedings of Society of Photo-optical Instrumentation Engineers 2001, 4472: 471–481.Google Scholar
- Rosenfeld A, Kak AC: Digital Picture Processing. 2nd edition. New York, Academic Press; 1982.Google Scholar
- Zhao C, York A, Yang F, Forsthoefel DJ, Dave V, Fu D, Zhang D, Corado MS, Small S, Seeger MA, Ma J: The activity of the Drosophila morphogenetic protein Bicoid is inhibited by a domain located outside its homeodomain. Development 2002, 129: 1669–1680.PubMedGoogle Scholar
- Gao Q, Finkelstein R: Targeting gene expression to the head: the Drosophila orthodenticle gene is a direct target of the Bicoid morphogen. Development 1998, 125: 4185–4193.PubMedGoogle Scholar
- Wimmer EA, Cohen SM, Jackle H, Desplan C: buttonhead does not contribute to a combinatorial code proposed for Drosophila head development. Development 1997, 124: 1509–1517.PubMedGoogle Scholar
- Schulz C, Tautz D: Autonomous concentration-dependent activation and repression of Kruppel by hunchback in the Drosophila embryo. Development 1994, 120: 3043–3049.PubMedGoogle Scholar
- Tsai C, Gergen JP: Gap gene properties of the pair-rule gene runt during Drosophila segmentation. Development 1994, 120: 1671–1683.PubMedGoogle Scholar
- Janody F, Reischl J, Dostatni N: Persistence of Hunchback in the terminal region of the Drosophila blastoderm embryo impairs anterior development. Development 2000, 127: 1573–1582.PubMedGoogle Scholar
- Sauer F, Wassarman DA, Rubin GM, Tjian R: TAF(II)s mediate activation of transcription in the Drosophila embryo. Cell 1996, 87: 1271–1284. 10.1016/S0092-8674(00)81822-XView ArticlePubMedGoogle Scholar
- Strunk B, Struffi P, Wright K, Pabst B, Thomas J, Qin L, Arnosti DN: Role of CtBP in transcriptional repression by the Drosophila giant protein. Dev Biol 2001, 239: 229–240. 10.1006/dbio.2001.0454View ArticlePubMedGoogle Scholar
- Colas JF, Launay JM, Vonesch JL, Hickel P, Maroteaux L: Serotonin synchronises convergent extension of ectoderm with morphogenetic gastrulation movements in Drosophila. Mech Dev 1999, 87: 77–91. 10.1016/S0925-4773(99)00141-0View ArticlePubMedGoogle Scholar
- Wu X, Vasisht V, Kosman D, Reinitz J, Small S: Thoracic patterning by the Drosophila gap gene hunchback. Dev Biol 2001, 237: 79–92. 10.1006/dbio.2001.0355View ArticlePubMedGoogle Scholar
- Ghiglione C, Perrimon N, Perkins LA: Quantitative variations in the level of MAPK activity control patterning of the embryonic termini in Drosophila. Dev Biol 1999, 205: 181–193. 10.1006/dbio.1998.9102View ArticlePubMedGoogle Scholar
- Pankratz MJ, Busch M, Hoch M, Seifert E, Jackle H: Spatial control of the gap gene knirps in the Drosophila embryo by posterior morphogen system. Science 1992, 255: 986–989.View ArticlePubMedGoogle Scholar
- Melnick MB, Perkins LA, Lee M, Ambrosio L, Perrimon N: Developmental and molecular characterization of mutations in the Drosophila-raf serine/threonine protein kinase. Development 1993, 118: 127–138.PubMedGoogle Scholar
- Parkhurst SM, Lipshitz HD, Ish-Horowicz D: achaete-scute feminizing activities and Drosophila sex determination. Development 1993, 117: 737–749.PubMedGoogle Scholar
- Zhou A, Hassel BA, Silverman RH: Expression cloning of 2–5A-dependent RNAase: A uniquely regulated mediator of interferon action. Cell 1993, 72: 753–765. 10.1016/0092-8674(93)90403-DView ArticlePubMedGoogle Scholar
- Niessing D, Dostatni N, Jackle H, Rivera-Pomar R: Sequence interval within the PEST motif of Bicoid is important for translational repression of caudal mRNA in the anterior region of the Drosophila embryo. Embo J 1999, 18: 1966–1973. 10.1093/emboj/18.7.1966PubMed CentralView ArticlePubMedGoogle Scholar
- Yagi Y, Suzuki T, Hayashi S: Interaction between Drosophila EGF receptor and vnd determines three dorsoventral domains of the neuroectoderm. Development 1998, 125: 3625–3633.PubMedGoogle Scholar
- Cowden J, Levine M: The Snail repressor positions Notch signaling in the Drosophila embryo. Development 2002, 129: 1785–1793.PubMedGoogle Scholar
- Miskiewicz P, Morrissey D, Lan Y, Raj L, Kessler S, Fujioka M, Goto T, Weir M: Both the paired domain and homeodomain are required for in vivo function of Drosophila Paired. Development 1996, 122: 2709–2718.PubMedGoogle Scholar
- Schulz C, Tautz D: Zygotic caudal regulation by hunchback and its role in abdominal segment formation of the Drosophila embryo. Development 1995, 121: 1023–1028.PubMedGoogle Scholar
- Goff DJ, Nilson LA, Morisato D: Establishment of dorsal-ventral polarity of the Drosophila egg requires capicua action in ovarian follicle cells. Development 2001, 128: 4553–4562.PubMedGoogle Scholar
- Sackerson C, Fujioka M, Goto T: The even-skipped locus is contained in a 16-kb chromatin domain. Dev Biol 1999, 211: 39–52. 10.1006/dbio.1999.9301View ArticlePubMedGoogle Scholar
- Rusch J, Levine M: Regulation of a dpp target gene in the Drosophila embryo. Development 1997, 124: 303–311.PubMedGoogle Scholar
- Steingrimsson E, Pignoni F, Liaw GJ, Lengyel JA: Dual role of the Drosophila pattern gene tailless in embryonic termini. Science 1991, 254: 418–421.View ArticlePubMedGoogle Scholar
- Hamada F, Bienz M: A Drosophila APC tumour suppressor homologue functions in cellular adhesion. Nat Cell Biol 2002, 4: 208–213. 10.1038/ncb755View ArticlePubMedGoogle Scholar
- Klinger M, Soong J, Butler B, Gergen JP: Disperse versus compact elements for the regulation of runt stripes in Drosophila. Developmental Biology 1996, 177: 73–84. 10.1006/dbio.1996.0146View ArticleGoogle Scholar
- Bashirullah A, Halsell SR, Cooperstock RL, Kloc M, Karaiskakis A, Fisher WW, Fu W, Hamilton JK, Etkin LD, Lipshitz HD: Joint action of two RNA degradation pathways controls the timing of maternal transcript elimination at the midblastula transition in Drosophila melanogaster. Embo J 1999, 18: 2610–2620. 10.1093/emboj/18.9.2610PubMed CentralView ArticlePubMedGoogle Scholar
- Verheyen EM, Mirkovic I, MacLean SJ, Langmann C, Andrews BC, MacKinnon C: The tissue polarity gene nemo carries out multiple roles in patterning during Drosophila development. Mech Dev 2001, 101: 119–132. 10.1016/S0925-4773(00)00574-8View ArticlePubMedGoogle Scholar
- Wolff C, Schroder R, Schulz C, Tautz D, Klingler M: Regulation of the Tribolium homologues of caudal and hunchback in Drosophila: evidence for maternal gradient systems in a short germ embryo. Development 1998, 125: 3645–3654.PubMedGoogle Scholar
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