Figure 2

Normalization for lane 12 h-1 in dissimilar range (35–50 bp) in Fig. 1 (Model 1). Magnified expression profiles of the target 12 h-1 and the reference 96 h-2 in the range in Fig. 1 are shown (top). Colours are the same as those in Fig. 1. There are 61 possible combinations in this case: 30 different levels of expansion (x = 0, 1, ..., 29), the original target profile (x = 30), and 30 compressions (x = 31, 32, ..., 60). The highest correlation coefficient between the best approximating profile and the reference in range (3–-50 bp) was 0.844 for the case x = 9. The position of x on the X axis corresponds to the new position of the short side (originally, 35 bp) of the original profile after expansion or compression. For example, the new position of the short side after maximum expansion (x = 0) becomes 29 bp, while after maximum compression (x = 60) it becomes 41 bp. Visual evaluation of three representative approximate profiles (x = 0, 9, and 60) in range (35–50 bp) confirmed the validity of the normalization (bottom).