Figure 6
From: Kismeth: Analyzer of plant methylation states through bisulfite sequencing
Design of primers for Bisulfite sequencing. Through this page the user can upload a reference sequence file, determine the length of the resulting product and the desired Tm, and Kismeth will provide a list of optional primers ranked by their predicted efficiency. The primers are designed taking into account bisulfite limitations, as described in the text. The user can also choose to design primers for the reverse complement of the input sequence, and thus interrogate both DNA strands.