Anatomy of enzyme channels
© Pravda et al.; licensee BioMed Central Ltd. 2014
Received: 19 July 2014
Accepted: 5 November 2014
Published: 18 November 2014
Enzyme active sites can be connected to the exterior environment by one or more channels passing through the protein. Despite our current knowledge of enzyme structure and function, surprisingly little is known about how often channels are present or about any structural features such channels may have in common.
Here, we analyze the long channels (i.e. >15 Å) leading to the active sites of 4,306 enzyme structures. We find that over 64% of enzymes contain two or more long channels, their typical length being 28 Å. We show that amino acid compositions of the channel significantly differ both to the composition of the active site, surface and interior of the protein.
The majority of enzymes have buried active sites accessible via a network of access channels. This indicates that enzymes tend to have buried active sites, with channels controlling access to, and egress from, them, and that suggests channels may play a key role in helping determine enzyme substrate.
Channels inside biomacromolecular structures (proteins, nucleic acids and their complexes) play many significant biological roles as they enable traffic between the interior spaces and the exterior. In enzymes they allow passage of substrates and products to/from the active site -, in the ribosome they allow nascently synthetized proteins to pass from the proteosynthetic center to the outside , and in membrane proteins they provide high specificity of passage in either direction through the membrane ,. Thus channels have attracted the attention of many researchers, who have rationalized their biological roles using a variety of experimental and theoretical methods. The ribosome, for example, prevents nascently synthetized polypeptides getting stuck in its polypeptide egress channel by lining the wall of the channel with a mosaic of alternating negative and positive electrostatic potentials ,. Gramicidin provides polar holes for biomembranes, enabling free diffusion of monovalent ions and water through the membrane -, while transmembrane ion channels maintain their high selectivity by a combination of structural and electrostatic features of the channel-lining amino acids ,.
Enzymes are proteins that catalyse reactions changing substrates to products. The enzymatic reactions occur in the enzymes’ active sites. Thanks to the many analyses of enzymatic reactions, we now have a better understanding of how active site chemistry works - and which amino acids are present in the sites . However, relatively little is known about how substrates enter active sites and how the respective products leave them. While some active sites are positioned on the protein's surface, in clefts or pockets, other enzymes have deeply buried active sites, which are connected to the outside by one or more channels. Here we focus on these channels, as the active site access paths play an important role in substrate and product trafficking between active site and outside. It has been shown that mutations in enzymes’ active site access channels alter the substrate preferences of haloalkane dehalogenase enzymes and may be utilized in rational design of enzymes ,. The amino acids lining the access channels of cytochrome P450 (CYP) are important for the selectivity of these enzymes  while the flexibility of these channels, i.e. their opening and closing motions, contributes to the broad substrate specificity of CYP ,.
Number of enzyme entries in each EC class, and numbers of channels of different lengths
Number of enzymes
Enzymes with channels of length
≥ 5 Å
≥ 15 Å
≥ 20 Å
Geometrical channel features for all enzyme classes
Physicochemical features of channels
-0.64 ± 0.05
14.2 ± 0.4
-0.97 ± 0.06
17.2 ± 0.4
-1.05 ± 0.05
17.2 ± 0.4
-1.05 ± 0.08
17.4 ± 0.6
-1.07 ± 0.11
17.9 ± 0.9
-1.30 ± 0.15
20.7 ± 1.3
-0.92 ± 0.03
16.5 ± 0.2
-1.11 ± 0.04
18.7 ± 0.3
-0.78 ± 0.03
15.4 ± 0.2
-1.25 ± 0.04
18.8 ± 0.3
The average channel polarity is 16.5, which falls between the values of highly polar amino acids (Asp, Glu, Lys, Arg and His having polarities of 49.5 - 52.0) and those of other amino acids (with polarities of 0.0 - 3.5). It indicates that the channels are rather polar as well as hydrophilic. Taking all this information into account we may conclude that the average channel has slightly negative hydropathy and higher polarity. However, highly hydrophobic and nonpolar, as well as highly hydrophilic and polar, channels were also detected. We also analyzed the presence of charged amino acid side chains (Asp, Glu, His, Lys and Arg) in channels walls (Additional file 1: Table S2). On average the channel walls are lined by two negative and two positive side chains resulting in sum neutral channel walls (Table 3).
We also identified channels with significant extreme physico-chemical properties (Additional file 1: Table S3 and Additional file 1: Figure S1). Here we present two examples. A highly hydrophilic channel (hydropathy index -3.8) of length 18.7 Å occurs in 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (PDB ID: 1N8F) from E. coli. The high hydrophilicity of the channel is in accord with its function  since this enzyme catalyses a condensation reaction between two highly polar substrates: phosphoenolpyruvate and erythrose-4-phosphate. It is worth noting that transferases (EC2) show the largest variability in hydrophobicity as illustrated by the fact that six times transferases are in the top 10 having highly hydrophilic channels, and four occur in the top 10 with highly hydrophobic channels.
At the other extreme is peroxidase (PDBID: 1LYK) from the fungus Coprinus cinereus  which has a highly hydrophobic channel (hydropathy index of 3.59) of length 23.8 Å. This channel enables transport of simple phenols and smaller aromatic dye molecules for their oxidation in lignin decomposition  in a process which has been exploited in biotechnology as a dye-transfer inhibitor in a laundry detergent .
These examples show that enzymatic classes differ in their average physico-chemical properties: (i) oxidoreductases (EC1) show the most hydrophobic as well as the least polar channels among the enzyme classes, while (ii) ligases (EC6), and to some extent also isomerases (EC5), lyases (EC4) and hydrolases (EC3), show the most hydrophilic as well as the most polar channels (Figure 2, Table 3 and Additional file 1: Table S3).
We also identified that some physicochemical features differ across the three channel layers: internal, middle and external (Table 3). The polarity of middle part of the channel is always lower than polarity of both internal and external parts, respectively. The lower polarity of the middle part of channel is also reflected by its significantly more hydrophobic behaviour. The charged residues occur mainly in external parts of enzyme channels, while the internal and middle part contains more aromatic residues.
Channel-lining amino acids are not uniformly distributed along the length of the channel. Internal parts of the channel tend to contain more aromatic residues (His, Trp, Tyr) together with cysteine (Cys), while glutamine and glutamate (Gln and Glu) and aliphatic amino acids (Pro, Ala, Leu) are underrepresented. These trends are similar to the catalytic site propensities, as discussed in the previous paragraph. The frequencies of amino acids in the middle regions of the channels correspond to the frequencies in the entire channel with the exception of glycine (Gly) and aromatic amino acids (Trp, Tyr, Phe), which are present more frequently. We may hypothesize that the higher frequency of glycine (Gly) in the middle channel parts is because it facilitates flexibility, which may be important for substrate/product channeling between the active site and protein surface , whereas aromatic amino acids can serve as gate-keepers. External parts of the channel bear more charged residues than any other part (Arg, Lys, Glu, Asp) together with proline (Pro) and glutamine (Gln), whereas other polar residues (Ser, Thr) are surprisingly less common in the external parts even though that both Ser and Thr are evenly distributed within the structure of proteins (Additional file 1: Figure S4). External parts of channels are less occupied by aliphatic (Ile, Ala, Val) and aromatic amino acids (Phe, Tyr) as well as typical catalytic amino acids - histidine (His), aspartate (Asp) and cysteine (Cys). A higher frequency of charged or rather bulky and aromatic amino acids in channels may have functional implications because such amino acids may work as gate-keepers, regulating traffic between active site and surface via conformational changes. It is worth noting that some mutations of such residues have been shown to alter the catalytic efficiency and substrate preferences in haloalkane dehalogenases .
All the results above concern channels leading to buried enzyme active sites. For comparison, we also analyzed all channels of length greater than 15 Å connecting a cavity in the protein interior to the outside exterior (Additional file 1: Figure S4). We compared the amino acid compositions of the two types of channels. The active site access channels have higher frequencies of aromatic (Tyr, Trp and Phe), polar amino acids (Asn, Thr and Ser), catalytically active amino acids (Cys and His), and glycine (Gly). On the other hand, they contain fewer aliphatic amino acids (Pro, Leu, Ile and Val), charged and some polar amino acids (Lys, Glu, Arg and Gln). In sum, the active site access channels contain more functional amino acids than generic channels. This finding agrees with the idea that some amino acids bear functional roles in channels, e.g., as gate keepers or to maintain their flexibility.
The analysis of amino acids leading to active sites, divided according to the six enzymatic groups, shows that amino acid channel propensities correspond to overall channel propensities. However, some differences were identified (Additional file 1: Figure S4). As can be expected from their higher hydropathy and lower polarity, channels in oxidoreductases (EC1) have significantly lower frequencies of charged lining amino acids (Arg, Asp, Lys, Glu), but higher frequencies of aliphatic lining amino acids (Met, Pro, Ile, Leu, Ala, Val). Channels in transferases (EC2) contain fewer aromatic (Trp) and more charged (Arg, Asp, Lys) amino acids. Channels in hydrolases (EC3) contain fewer arginine (Arg), threonine (Thr) and aliphatic amino acids (Pro, Ile, Ala, Val), whereas they contain more aromatic (Trp, Tyr) and smaller charged (Lys, Asp, Glu) amino acids. Channels in lyases (EC4) show only lower amounts of aromatic (Trp) and sulphur containing (Met, Cys) amino acids. Channels in isomerases (EC5) contain fewer glycines (Gly). Channels in ligases (EC6) are the most hydrophilic channels, so it is not surprising that their channels contain fewer cysteine (Cys), aromatic (Trp, Tyr) and aliphatic (Pro, Leu) amino acids and more charged (Arg, Lys, Glu) amino acids and glycine (Gly). It should be noted that the differences between the individual enzyme classes should be interpreted with care because of larger statistical error bars, especially in the case of less populated EC5 and EC6 classes.
Long channels (>15 Å) are a common feature of enzymes, with over 64% containing at least two such channels. This shows that the majority of enzymes have buried active sites accessible via a network of access channels. Hence there is an apparent tendency for enzymes to bury their active site, i.e., to limit and control direct connection of active sites with the surrounding environment. This may be the result of two evolutionary pressures; i) steric, because active sites have to be structurally well arranged - a buried active site enables full spatial arrangement better than a pocket-like active site can give that half of the space of the latter is open to surrounding environment and ii) functional, as active site access paths may enable pre-selection of substrates, and may be involved in features co-determining enzyme substrate preferences. In another words, the active site access channels may limit access to the enzyme active sites and function as keyholes, enabling passage only of some classes of substrates.
The amino acid frequencies in the whole protein structures and channel walls differ significantly. Aliphatic amino acids are more involved in the formation of enzyme hydrophobic cores, which are important to maintain a protein fold. In turn, they are less frequently involved in channel wall lining or within the active sites. The aromatic, charged and polar amino acids occur more frequently in the channels walls. In addition, we identified a higher frequency of glycine in the middle parts of channels, which may function here to support channel flexibility enabling the channelling of bulkier substrates to active sites. This finding can be explained by the fact that the polar and charged amino acids line the channels to enable passage of polar substrates/products and water. The enhanced frequency of rather bulky and aromatic amino acids in channel external parts may have functional implications, because such amino acids may work as gate-keepers, regulating traffic between active site and outside.
The functional implications deduced from these global analyses are also supported by the fact that individual enzyme classes differ in their channel features. Typically oxidoreductases have the most hydrophobic, the least polar and longest channels among the enzyme classes, while ligases have the most hydrophilic, the most polar and the shortest channels. This indicates that evolution of enzymatic substrate preferences might also include evolution of active site access channels.
To conclude, we analyzed channels in 4,306 enzyme structures annotated in the Catalytic Site Atlas. We identified that at least 64% of enzyme structures contain on average two channels longer than 15 Å leading to the catalytic site. Consequently, we may anticipate that the same number of enzymes have buried active sites. The longest, and also the most hydrophobic, channels are found in oxidoreductases, while the smallest number of channels can be found in hydrolases and the shortest and also the most hydrophilic channels in ligases. The composition of channel walls differs from the average composition of enzyme structures as well as from the composition of the protein surface. Hydrophobic aliphatic amino acids, which are the most common amino acids present in protein cores, occur in channel walls less frequently, whereas aromatic, charged and polar amino acids occur more frequently in channel walls. All these findings indicate that the active site access channels bear significant biological function as they are involved in co-determining enzyme substrate preferences.
We analyzed 4,306 enzymes which were annotated in the Catalytic Site Atlas (CSA) database release of 4th March 2013 ,. The dataset contained structures determined by X-ray diffraction at a resolution better than 2.5 Å, and had no two structures with a sequence identity higher than 90% (more quality checks can be found in Additional file 1: Table S1). It should be noted that when we used a dataset containing structures with a sequence identity less than 50%, the results did not significantly differ from the results obtained with the dataset containing structures with a sequence identity less than 90%. The enzymes in the dataset were grouped according to their Enzymatic Commission (EC) class (Table 1).
An active site is a cavity, which walls contain amino acids residues annotated in the CSA. A channel is a pathway inside an active site cavity connecting the deepest apex of the cavity with an exterior. The MOLE 2.0 program  was used for channel identification and characterization. Briefly, the MOLE 2.0 algorithm calculates the Delaunay triangulation/Voronoi diagram of the atomic centers, splitting it into several smaller parts and identifying suitable start and end points in the interior and surface, respectively. Dijkstra's algorithm is used to identify tunnels as the shortest paths between the start and end points (see Additional file 1 for further details). This algorithm is used also in the MOLEonline 2.0 web application . The setup of MOLE 2.0 for these calculations was as follows: Probe Radius and Origin Radius 5 Å, Interior Threshold 1.1 Å and default values for Bottleneck Length, Bottleneck Radius, Cutoff Ratio and Surface Cover Radius. The CSA active sites were used as starting points. We used biological assemblies for the enzymes structures, which were obtained from the PDB database as *.pdb1 files.  Ten structures of EC 3.6.4 group were removed from the dataset. Hydrogen atoms and ligands not covalently bound to the structure were deleted prior to calculation. In cases where the system contained more than one active site, the site having the most channels was used. In order to study only relevant channels, we analysed only those channels longer than 15 Å. Table 1 shows the numbers of channels of different lengths for each of the six different enzyme classes, which are listed together with their properties in Additional file 2.
In the text we use the following terminology (Figure 1); Lining amino acids are all the amino acids fully encapsulating the detected channel and are divided into three classes: internal, middle, and external according to their respective positions in the layers perpendicular the channel centerline. Internal or external lining amino acids are those lying within a 5 Å distance of the start or end point, respectively, with middle amino acids constituting the remainder. A bottleneck is where the channel radius is a minimum.
This work was supported by the Czech Science Foundation [P208/12/G016 to M.O., P303/12/P019 to K.B.] and the Operational Program Research and Development for Innovations-European Regional Development Fund [CZ.1.05/2.1.00/03.0058 to M.O., K.B., P.B. and CZ.1.05/1.1.00/02.0068 to J.K. and R.S.V.], European Social Fund [CZ.1.07/2.3.00/20.0058 to K.B.]. D.S. acknowledges Brno City Municipality for financial support provided through the program Brno Ph.D. Talent. Access to the MetaCentrum supercomputing facilities provided under the research intent MSM6383917201 is gratefully acknowledged.
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