An algorithm to enumerate all possible protein conformations verifying a set of distance constraints
 Andrea Cassioli^{4},
 Benjamin Bardiaux^{1, 2},
 Guillaume Bouvier^{1, 2},
 Antonio Mucherino^{6},
 Rafael Alves^{4},
 Leo Liberti^{4, 5},
 Michael Nilges^{1, 2},
 Carlile Lavor^{3} and
 Thérèse E Malliavin^{1, 2}Email author
https://doi.org/10.1186/s1285901504511
© Cassioli et al.; licensee BioMed Central. 2015
Received: 9 July 2014
Accepted: 5 January 2015
Published: 28 January 2015
Abstract
Background
The determination of protein structures satisfying distance constraints is an important problem in structural biology. Whereas the most common method currently employed is simulated annealing, there have been other methods previously proposed in the literature. Most of them, however, are designed to find one solution only.
Results
In order to explore exhaustively the feasible conformational space, we propose here an interval BranchandPrune algorithm (iBP) to solve the Distance Geometry Problem (DGP) associated to protein structure determination. This algorithm is based on a discretization of the problem obtained by recursively constructing a search space having the structure of a tree, and by verifying whether the generated atomic positions are feasible or not by making use of pruning devices. The pruning devices used here are directly related to features of protein conformations.
Conclusions
We described the new algorithm iBP to generate protein conformations satisfying distance constraints, that would potentially allows a systematic exploration of the conformational space. The algorithm iBP has been applied on three αhelical peptides.
Keywords
Background
Protein structure determination is crucial for understanding protein function, as it paves the way to the discovery of new chemical compounds and of new approaches to control the biological processes.
The problem of protein structure determination is certainly a problem with multiple solutions, as proteins are flexible polymers. As most of the experimental techniques of the structural biology obtain measurements averaged on an ensemble of protein conformations, the usual approaches for structure determination intend to find an average structure or a set of conformations describing fluctuations around an average structure. A path intending to get a complete coverage of the conformational space, given a series of constraints, is usually not taken, although such an approach could provide precious information about the conformational equilibrium, which is essential in the function of many proteins, as the HIV protease [1].
An important class of experimental methods for protein structure determination is based on the measurement of interatomic distances and angles, such as Nuclear Magnetic Resonance (NMR) spectroscopy [2] and crosslinking coupled to mass spectrometry [3]. In NMR, distance intervals between hydrogens are determined from the measurement of nuclear Overhauser effects (NOE). The experimentally measured distances are then used as constraints for protein structure calculations. Pure in silico approaches have been also developed based on the use of interatomic distance constraints, such as homology modeling [4] or prediction of proteinprotein complexes [5] and ligand poses [6].
The Distance Geometry Problem (DGP) [7,8] consists in identifying the sets of points which satisfy a set of constraints based on relative distances between some pairs of such points. The present work describes an algorithm developed to solve DGP in the context of protein structure determination: the points represent the protein atoms.
The DGP is a constraint satisfaction problem. Several approaches solve this problem by reformulating it [8] as a global optimization problem having a continuous search domain, and whose objective function is generally a penalty function designed to measure the violation of the distance constraints. Over the years, the solution of DGPs arising in structural biology have been typically attempted by Simulated Annealing (SA) approaches based on molecular dynamics [9]. Other proposed approaches are based on various optimization methods as in [10]. As all metaheuristic approaches, SA may provide approximate solutions but does not deliver optimality certificates. In the case of protein structure determination, since the optimization problem is a reformulation of a constraint satisfaction problem, solutions given by SA can be successively verified by checking the violations of the distance constraints. However, additional solutions may exist but go undetected by SA. Thus, an algorithm for the systematic enumeration of the possible conformations of a given protein could find a widespread field of application. Branchandprune algorithms and similar were proposed in the general context of protein structure determination [1116], (see also [8] and references therein). However, these studies primarily addressed the question of defining relative orientations of protein monomers in symmetric oligomers, not the determination of all possible conformation of a polypeptide chain with a very large number of degrees of freedom from distance constraints. Systematic exploration was proved to be useful in the case of residual dipolar couplings (RDC) constraints [17], for exploring the sidechains conformations [18,19] and for assignment of NOEs, provided that the structure is known [20]. For the structure determination from RDCs, it has been shown [21] that when using RDCs but only sparse NOEs the problem can be solved in polynomial time. Such approaches have also been used for structure determination in Xray crystallography for noncrystallographic symmetry by orienting and translating symmetric protein subunits [22]. To the best of our knowledge, in this paper we present the first application of a BranchandPrune algorithm to the problem of full protein structure determination based on unambiguous distance information.
In the present work, we first describe the branching phase and the pruning devices used to determine the solutions to the Distance Geometry problem. Then, an overall view of the method is given along with the use of the branching and pruning devices at different steps and the complexity of the algorithm is analyzed. We finally illustrate the algorithm application with three proteins for which αhelical regions are known along with few longrange NMR constraints (ie. constraints measured between residues i and j such that i−j>3 in the protein sequence). The obtained conformations display good stereochemical quality parameters, and the conformational space explored is larger than the one sampled with traditional optimization methods such as simulated annealing.
Methods
In order to sample the conformational space of a protein, we use a BranchandPrune algorithm to build a tree in which each node represents a solution for one atomic position. We limit ourselves in the present work to the calculation of the backbone and C β atomic coordinates.
 1.
covalent distance constraints corresponding to bond lengths and bond angles, whose values are derived from highresolution small molecule Xray crystal structures [25];
 2.
NMR distance constraints;
 3.van der Waals radii of atoms between nonbonded atom pairs (i,j): a fraction of the sum of the van der Waals radii of each atom provides a lower bound to the corresponding interatomic distances:$$ d_{ij}\geq \sigma (r^{vdw}_{i} + r^{vdw}_{j}), $$(1)
 4.
distances derived from the backbone torsion angles ϕ and ψ;
 5.
hydrogen bonds in αhelix;
 6.
aminoacid chirality;
 7.
αhelix geometry.
Then, the tree is built using a recursive procedure to create each node of the tree. This procedure is called branching phase. The created nodes are then submitted to the pruning devices in order to decide whether the node should be kept or removed. If the node is removed, the possible branches starting from this node are also pruned. A pruning device is responsible for checking whether a partial solution is feasible, i.e. to check whether a set of embedded atoms fulfill the constraints (1)(7) described above.
In the following, we describe the branching phase and the pruning devices. Then, the complexity of the algorithm is described from a theoretical point of view, before presenting some application cases.
Branching devices
The tree parsed during iBP is formed by nodes, each corresponding to one set of atomic coordinates from the order P _{ato} (Figure 3) [24]. At each level of the tree, the atomic coordinates of the corresponding atom are calculated by making use of a recursive procedure, called branching phase. The current atom position is defined by distance constraints to three other atoms. These distances are obtained from the constraints (13) described above: (1) the covalent constraints, (2) the NMR distance constraints, (3) the van der Waals radii.
In this case, we have a bbranching situation.
The algorithm used for calculating the atom coordinates is then applied to each set of \(\tilde {d}_{i}\) values sampled for the distance constraints. The choice of the discretization factor b is a crucial point: a small value might lead to an infeasible problem because we may not select any feasible distance; a larger value increases the computational burden. In general, the finer the discretization, the more accurate the computation is, but it is not trivial to figure out the optimal value for b. One way to choose b is to consider that the number of nodes in the search tree is bounded by 3+(2^{ l } b ^{ k }), where l is the number of tree levels where we have a 2branching situation, and k is the number of tree levels where we have a bbranching situation [28]. Appropriate values of b should result in a manageable number of nodes.
Given the position of the three previous atoms k−3, k−2, k−1 in the order P _{ato} and given the constraints to these atoms of the atom k to be embedded, the position of k is calculated by a recursive matrix multiplication by making use of the set of distances d={d _{ k,k−1},d _{ k,k−2},d _{ k,k−3}} between the previous atoms and k. Although there are several methods to compute sphere intersections [29], in our experience, the best tradeoff between efficiency and numerical stability is given by the use of recursion matrices [23], and of the two following angles: (i) the torsion angle ω _{3} formed by atoms {k,k−1,k−2,k−3} which depends on the distance between k and k−3, (ii) the angle θ _{2} formed by atoms {k,k−1,k−2}.
d _{2,1} being the distance between the first and the second atom, and d _{3,2} the distance between the third and the second atom in the order P _{ato}.
The total number of B _{ k } matrices to be calculated along the parsing of the tree is bounded by 2∣ P _{ato} ∣b, where ∣ P _{ato} ∣ is the size of the ordered atom list P _{ato}. The product Q _{ k−1} B _{ k } is calculated in two steps: (1) the fourth column of Q _{ k }, which gives us the coordinates of k, is computed; (2) only if k is not pruned, the three remaining columns are computed.
We must distinguish two cases when embedding an atom k. If it is the first appearance of k in P _{ato}, we use equation 3 to compute all possible embeddings of k for σ∈{+1,−1} and the set of distances d. If it is not the first appearance of k in P _{ato}, we need to take into account the fact that numerical instabilities generate matrices which will lead to slightly different coordinates for k than those computed the first time. In order to decrease the impact of these numerical errors, we compute the set of distances d, the angles θ _{2},ω _{3} and for σ∈{+1,−1} the corresponding matrices B _{ k }(d,+1),B _{ k }(d,−1), which lead to two possible embeddings of k (Equation 3), as k ^{+}=Q _{ k−1} B _{ k }(d,+1) and k ^{−}=Q _{ k−1} B _{ k }(d,−1). We choose the value of k that yields the updated coordinates of k being the closest to the previous coordinates of this atom.
Each carbonyl oxygen O ^{ i−1} is uniquely determined for residue i, once C ^{ i−1}, N ^{ i } and H ^{ i } have been embedded, since these atoms are all part of the peptide plane [30]. As is common practice (see, e.g., [3133]), we fix here the torsion angle ω of the peptide plane to 180° or 0°. In a previous implementation [34], the positions of the carboxylic oxygens were not stored. Although this approach leads to memory savings, the availability of carboxylic oxygen positions can improve the definition of the αhelix secondary structure.
The parameter d _{ k1} is the length of the bond connecting O ^{ i−1} and C ^{ i−1}, the parameters d _{ k2} and d _{ k3} are the distances between k=O ^{ i−1} and N ^{ i }, H ^{ i }, calculated from bond angles and bond lengths between atoms of the peptide plane, and the angle ω of 180° in a trans peptide plane. The case of the cis peptide plane can be treated in the same way, modifying the value of ω to be 0°.
The parameter d _{ k1} is the length of the bond connecting k=C β and C α, the parameters d _{ k2}, d _{ k3} and d _{ k4} are the distances between k=C β and H α, N, C, calculated from bond angles and bond lengths between these atoms.
Pruning devices
Once the set of possible coordinates of the atom k has been determined in the branching phase described above, pruning devices are used to check whether the coordinates of k are feasible. In some cases described below, the coordinates of k along with the coordinates of previously embedded atoms are checked together. If the check is negative, the solution obtained for k is discarded, which prunes all tree branches originating from the node k. In this section, we present the pruning devices used to accept or discard the coordinates of the atom k generated by the branching devices. The pruning device applies all these tests as soon as the involved atoms have been embedded.
Direct distance feasibility (DDF)
As the coordinates for an atom k are determined, we first check that all distances between k and the other embedded atoms respect the given lower and upper bounds arising from the constraints (13) listed in section “Solving the DGP with iBP”.
Torsion angle feasibility (TAF)
Taking the maximum and minimum values of d(ξ _{ ijkl }) for ξ _{ ijkl }∈Ξ _{ ijkl }, we obtain an interval [ l _{ il },u _{ il }] for the distance d _{ il }. The sign of the angle ξ _{ ijkl } is used as an additional pruning criterion along with the d _{ il } interval.
Dijkstra shortestpath (DSP)
As introduced in [23], we can exploit the fact that the distances are Euclidean to improve the iBP pruning capabilities. We extend and generalize the procedure presented in [36] in the following way. We introduce an auxiliary graph G ^{+} with the same topology as the graph connecting the atoms in the protein, but such that the weight of each edge (i,j) is the upper bound of the distance d _{ ij }. For every pair of atoms i,j, the shortestpath between i,j in G ^{+} is a valid overestimate of d _{ ij }. Thus we used an alltoall shortestpath algorithm, the FloydWarshall algorithm [37], to refine the upper bound for each pair of atoms.
where u _{ jk } is the upper bound of the atom pair (j,k) obtained using the FloydWarshall algorithm [37].
Chirality (CHI)
The pruning of atom coordinates through the aminoacid chirality is implemented through the socalled CORN rule of thumb: in amino acids, the groups COOH, R (sidechain), NH2 and H are bonded to the chiral center C α carbon. Starting with the hydrogen atom away from the viewer, if these groups are arranged clockwise around the C α carbon, then the aminoacid is in the Dform. If these groups are arranged counterclockwise, the aminoacid is in the Lform. The CORN rule was restated by imposing that the torsion angle defined by the atoms C,C β,N,H α of residue i for the Dform or C,N,C β,H α of residue i for the Lform, is positive.
αhelix secondary structure
We proposed the use of α helix information as a pruning device in the context of the iBP algorithm first in [34]. The α helix location can be determined from an analysis of the NMR chemical shifts by TALOS [38]. Four criteria are used to enforce the formation of an α helix: (i) the formation of backbone hydrogen bonds between amide hydrogens and carbonyl oxygens, (ii) the alignment of the amide and carbonyl functions checked by a qualitative condition on the energy of the hydrogen bond, (iii) the definition of backbone ϕ and ψ torsion angles already described in the Torsion Angle Feasibility, (iv) the definition of three additional angles θ, θ’ and θ” similar to the ones introduced by Grishaev et al. [39].
On a sequence of m+1 contiguous residues I _{ α }={i,i+1,…,i+m} forming an α helix, for any pair of residues (i−4,i) belonging to I _{ α }, the lower and upper bounds on the distance between the carboxylic oxygen O ^{ i−4} and the amide hydrogen H ^{ i } should be compatible with the formation of an hydrogen bond. The upper and lower bounds are defined in an input parameter file of iBP, and were set to 1.9 and 3.0 Å in the present work.
with q _{1}=0.42,q _{2}=0.2 and f=332, and d _{ AB } correspond to the distance between atoms A and B.
The last criterion enforces the angles θ, θ’, θ” to be respectively into the interval values 0/70°, 0/90° and 110/180°.
Implementation details
In this section we provide an overview of the main implementation features. The iBP algorithm has been coded in C++ with extensive use of template metaprogramming [41], STL [42,43], and BOOST (www.boost.org). Linear systems, as for instance (7), are solved using the LAPACK library [44].
Discretizable DGP instances were represented by simple weighted undirected graphs G=(V,E,d), which were handled by the Boost Graph Library (BGL) [45]. The points in \(\mathbb {R}^{3}\) were represented using the Boost Geometry Library (also known as Generic Geometry Library, GGL: www.boost.org).
The Boost Interval Library (BIL – see [46,47]) was used to store such representation, and to perform basic operations for intervals and singletons. On top of the BIL, we define the type domain which contains a set of intervals and operations as intersection, scaling, etc. The BIL allows also to select the underlining data format for the interval (single/double precision real, integer).
Theory
In this section we give some details about the worstcase asymptotic complexity behavior of the iBP algorithm. The description given above includes many details which are useful for finding the structure of proteins but which somewhat complicate the precise mathematical treatment. We first give a very brief abstract description of the iBP and of the formal problem it solves, and then proceed to discuss its complexity.
Formally speaking, the DGP is the following decision problem: given an integer K>0, a simple undirected graph G=(V,E) and an edge weight function \(d:E\to \mathbb {R}_{+}\), is there a realization \(x:V\to \mathbb {R}^{K}\) such that for each {u,v}∈E we have ∥x _{ u }−x _{ v }∥_{2}=d _{ uv }? Note that we are writing x _{ u } for x(u) and d _{ uv } for d(u,v). We also remark that in the more “applied” interpretation given in the preceding section, the range of the edge function d is \(\mathbb {IR}_{+}\), i.e. the set of all nonnegative closed real intervals, and K=3. The DGP is NPhard for any K>1 and NPcomplete for K=1 [48]. Since we are interested in finding all solutions of the DGP rather than just one, we denote by X the set of all realizations of G.
Assumptions on the DGP input data
 1.
there is an order 1,2,…,n on the vertices such that 1,2,3 is a triangle in the graph G and, for each vertex v>3, v is adjacent to v−1,v−2,v−3;
 2.
the set of edges E can be partitioned in two subsets E _{ D } and E _{ P }, such that E _{ P } consists of all edges {u,v} with v>4 and v−u>3, and \(E_{D}=E\smallsetminus E_{P}\);
 3.
E _{ D } can be further subdivided in \(E_{D}^{\prime }\) and \(E_{D}^{\prime \prime }\), so that \(E_{D}^{\prime \prime }\) consists of all edges {u,v} with v−u=3, and \(E_{D}^{\prime }=E_{D}\smallsetminus E_{D}^{\prime \prime }\);
 4.
the distance function d is such that: (a) d _{ uv } is a scalar for each \(\{u,v\}\in E^{\prime }_{D}\); (b) d _{ uv } consists of a discrete set of b scalars for each \(\{u,v\}\in E^{\prime \prime }_{D}\); (c) d _{ uv } is a general interval for all {u,v}∈E _{ P }.

we consider that distances which are known because of covalent bond relations are sufficiently precise to be represented by a scalar;

we consider that distances which are known from NOESY (or other) experiments can be represented by intervals;

we assume that a limited number of the intervals can be discretized into sets containing a finite number b of values within the intervals;

the edges in \(E_{D}^{\prime }\) represent atom pairs of the form {v,v−1} or {v,v−2} for any v>2: these are involved in covalent bonds;

the edges in \(E_{D}^{\prime \prime }\) represent atom pairs which are assigned a certain number b of possible values (optionally b=1 for certain pairs);

the edges in E _{ P } represent atom pairs for which the distance might be a general interval.
We remark that the order on V was initially intended to follow the protein backbone [49], but new orders which better exploit the hydrogen atoms in or close to the backbone have been defined in [50,51]: these are the orders on which the above assumptions are based.
The DGP with the restrictions above, but where all intervals are replaced by scalars, is called DISCRETIZABLE MOLECULAR DGP (DMDGP). Both the DMDGP and its generalization to any K (denoted by ^{K}DMDGP) are NPhard [52,53]. The problem defined above, involving intervals, obviously contains the DMDGP as a subcase and is hence also NPhard by inclusion.
When all distances are precise
We first focus on the simplest case, where all intervals are replaced by scalar values. Then \(d:E\to \mathbb {R}_{+}\), and b=1. In this simplified setting, the iBP is simply called BP [52], and the order on V is called a contiguous trilateration order [54] or a DMDGP order [55].
The BP can be defined as a recursive procedure: assuming we already found a realization x _{1},…,x _{ v−1} for the vertices 1,…,v−1, and that we mean to find a consistent realization x _{ v } for v, the discretization edges E _{ D } guarantee that there will be at most two positions for x _{ v } compatible with the distances restricted to E _{ D } [49]. This can be intuitively understood in \(\mathbb {R}^{3}\) by considering the intersection of three spheres centered at x _{ v−1},x _{ v−2},x _{ v−3} with radii d _{ v,v−1},d _{ v,v−2},d _{ v,v−3}: the first two spheres either do not meet or their intersection is in general a circle, and the intersection of the third sphere with this circle is either empty or consists in general of two points [56]. We can now consider the distances defined on pruning edges in E _{ P }, linking v to its preceding vertices in order to accept or reject these two points. For each accepted point we recursively call BP with v replaced by v+1, for all v<n. When v=n we have a valid realization of the graph: we save it in X, and proceed to complete the recursive search. This yields a search tree which is explored depthfirst. The recursion starts after placing the initial triangle 1,2,3 (either arbitrarily or by using BP restricted to subspaces), so this tree starts branching at level 4. It can be proved that, at completion, X contains all incongruent (modulo translations and rotations) realizations of G.
In the case where \(E_{P}=\varnothing \), the search tree is a complete binary tree with 2^{ n−3} nodes at the nth (and last) level: in other words, its depth is n and its width is 2^{ n−3}. This is the worst case, since the BP must explore all of the nodes in the tree, and proves that the BP (and hence the iBP, since it generalizes the BP) is an exponentialtime algorithm in n.
When \(E_{P}\not =\varnothing \), it was shown that X almost always contains a number of solutions which is either zero or a power of two [55]; this discovery led to a set of results where the BP search tree width can be kept polynomial in n during the search [53]. Since the exponential behavior is only due to the tree width, this yields a set of cases where the BP is actually fixedparameter tractable (FPT). Throughout all our experiments with protein data we were always able to fix the parameter controlling the exponential growth of the tree width to a universal constant, which makes BP “polynomial on proteins” (this is an informal statement — the precise statement is given in [53]).
Intervals and discrete distance sets
The theory supporting the case where d might map edges to discrete sets of distance values or intervals, which is the case treated in this paper, is not so clearly understood yet. As it generalizes the simpler case sketched above, in a certain sense it inherits its properties, but this is an oversimplification: for instance, if all intervals are [ 0,∞], it is obvious that the problem is easy independently of the graph topology, since every realization is valid.
Some bounds on the cardinality of X in the presence of discrete sets and intervals are given in [55]. Our understanding is that if the intervals are small enough, the theory which led to fixedparameter tractability goes through with few changes, but we have no way so far of establishing an aprioristic maximum width for the intervals. If the intervals are very large the problem might become tractable, as mentioned above, for the purposes of finding at least one solution. The iBP would still behave exponentially, however.
Results and discussion
We applied the presented algorithm to three examples of proteins displaying α helical secondary structures. Before presenting the obtained results, we emphasize that the method proposed here has a completely different philosophy than classical optimization approaches commonly used in the field of NMR structure determination. In the present approach, each constraint is treated in the strict sense, that is, no violation, however small, is tolerated. This is why we consistently use the word constraint in the paper. This is what potentially allows us to systematically explore the entire search space. However, the use of the procedure demands that the data have been preprocessed accordingly, and all geometric inconsistencies that exist in three–dimensional space have been removed.
For the proteins studied here, if one includes the ensemble of NMR interval distance constraints stored in the.mr file at the Protein Data Bank (PDB) [57] as well as all pruning devices described above, all solutions are pruned out, indicating that no solution to the distance geometry problem exists with the deposited data. This is not really surprising, since the optimization algorithms generally used in NMR structure determination are based on optimization of a target function or hybrid energy rather than on strict constraint satisfaction. That is, there is always a phase where the algorithm tries to find a tradeoff when inconsistencies exist between constraints. The optimization thus produces solutions in which chemical and NMR constraints are optimized, but in which small violations are always present. These inconsistencies are present in any structure determination, in particular because distance constraints are imprecise, due to experimental limitations.
Analysis of conformations obtained by the branchandpruning algorithm on the three proteins targets: 2JMY, 2KXA and 2KSL
Proteins  2JMY  2JMY_1  2JMY_2  2KXA  2KSL 

Number of  
residues  15  15  15  24  51 
Number of  
vertices  107  107  107  170  359 
Definition  
of α helices  115  313  511  111, 1323  411, 1327, 
2936, 4150  
Position  
tolerance (Å)  0.2  0.2  0.2  0.2  0.2 
Angle  
tolerance (°)  2  2  2  4  4 
b value  4  4  4  8  4 
Number of  
longrange  
constraints  0  0  0  1  3 
Number of saved  
conformations  1  10000  10000  10000  10000 
Number of generated  
conformations  1  633,937  928,399  3,380,964  491,498 
CPU time    1 min  1 min  25 min  31 min 
Number of violated  
constraints (> 1Å)  0  4.0 ± 2.1  11.6 ± 3.6  9.6 ± 2.9  12.8 ± 1.1 
Maximum  
violation (Å)  0  3.3 ± 1.4  4.8 ± 0.7  3.7 ± 1.0  8.1 ± 0.6 
Mininum RMSD  
from PDB structure (Å)  1.4  1.3  2.1  1.1  3.0 
RMSD from PDB structure  
for minimum violated  
conformations (Å)  1.4  2.9  2.8  1.3  3.5 
PROCHECK  
core residues (%)  100  65.7 ± 25.9  49.2 ± 7.6  60.4 ± 8.1  76.9 ± 2.4 
allowed residues (%)  0  17.9 ± 9.7  40.9 ± 8.3  39.6 ± 8.0  21.3 ± 2.8 
gen.allow. residues (%)  0  3.6 ± 4.8  9.9 ± 7.2  0.0 ± 0.0  1.9 ± 1.7 
disall. residues (%)  0  0.0 ± 0.0  0.0 ± 0.0  0.0 ± 0.0  0.0 ± 0.0 
The three examples we chose to illustrate the algorithm display an increasing structural complexity: (i) a single α helix, corresponding to the structure of peptide CM15 determined in micelles (PDB id: 2JMY [58]), (ii) an α helical hairpin (PDB id: 2KXA [59]), (iii) the insecticidial toxin TAITX1a, formed as a bundle of four α helices, restrained by three disulphide bridges (PDB id: 2KSL). The main characteristics of the studied proteins are given in Table 2. All three examples were originally determined by NMR spectroscopy, and the corresponding constraint lists are available from the PDB. The analysis by PROCHECK [60] of the Ramachandran diagram of these three PDB structures shows that more than 85% of the residues are located in the core region. For 2KXA and 2KSL, more than 95% of the residues are located in the core and allowed region, whereas in 2JMY, 7% of the residues are located in the generously allowed region. For 2KXA, one PRO residue was replaced by an ALA, as the PRO cycle has not yet been included in the current version of the iBP algorithm.

for 2KXA, one constraint between H α hydrogen and carbonyl oxygen of Ala5 and Met17, enforcing the pairing of the two αhelices,

for 2KSL, three constraints between Carbons β of Cys7 and Cys37, of Cys23 and Cys33 and of Cys26 and Cys46, corresponding to the formation of the three disulphide bridges.
For all calculations, except the one of 2JMY with the α helix defined along the whole sequence, the obtained conformations were filtered according to the coordinate root meansquared deviation (RMSD: 1.5 Å) with respect to the previously obtained conformation in the iBP procedure. Enforcing an RMSD value larger than 1.5 Å between two successively stored conformations, avoids an oversampling of the conformational space. Each calculation was stopped after storing 10000 filtered conformations. For our three examples, five calculations were performed in total: three on 2JMY with different definitions of the α helix (residues 115, 313 and 511), and one each for 2KXA and 2KSL. For the first calculation on 2JMY, one conformation was obtained and saved. The second and third calculations on 2JMY were quite short, of the order of minutes (Table 2), which is due to the small size of the corresponding tree. For the 2KXA and 2KSL calculations, 10000 conformations were obtained in about 30 mins of calculation. Large total numbers of conformations were generated: this number increases from ∼634,000 (2JMY_1) up to ∼3,400,000 (2KXA) with the size of the considered problem, depending on the number of residues and on the number of constraints. Despite 2KSL being the largest example, the second smallest number of conformations was generated, which is the sign of a severe pruning arising from a rather restricted conformational space.
The reliability of the obtained conformations was checked in three ways. First, the whole set of NMR constraints deposited along with the PDB entries and involving backbone hydrogens, were probed on the conformations. Second, the quality of the obtained conformations was checked using PROCHECK [60] analysis of the Ramachandran plot. Third, the obtained conformations were clustered with an unsupervised clustering method, namely the selforganizing map or SOM [6164], in order to investigate the properties of sampled conformations.
The agreement of the obtained conformations with the backbone NMR constraints deposited with the PDB structures was checked by calculating the distances between the backbone hydrogens in each obtained conformation. The distances larger than the upper bound of the constraint correspond to violations of this constraint. The mean number of violated constraints along with the mean value of the difference to the upper bound for these constraints were calculated on all conformations (Table 2). For the 2JMY calculation with the 115 α helix definition, no violation of the NMR constraints could be observed. As expected, when the α helix definition is reduced (2JMY_1 and 2JMY_2), the average number of violations increases as well as the average maximum violation. Not surprisingly, the most violated constraints involve residues located at the N and C terminal parts of the αhelix, TRP2, PHE5, LYS3, LYS6 and VAL11, VAL14, LEU15 for 2JMY_1 and 2JMY_2. The largest violations and number of violations are of the same order or value for 2KXA than for 2JMY_1 and 2JMY_2. In contrast, the largest violations and number of violations are observed for 2KSL and involve residues CYS33, GLU34, PHE38, TYR43. Such overrestraining of NMR structures have been put in evidence in the past, through molecular dynamics simulations [65] and analysis of the structure quality [66].
The average number of violations is similar for 2JMY_2, 2KXA and 2KSL, but the average maximum violation for 2KSL is twice as large as that for 2JMY_2 and 2KXA. This might be due to the very restrained conformations of 2KSL, which contain three disulphide bridges. Due to this restrained conformation, the NMR constraint list is probably more prone to contain inconsistencies, and large mechanical strain can be stored in the structure if one uses an optimization procedure such as simulated annealing. In contrast, no mechanical strain whatsoever is generated by the iBP algorithm, and the obtained conformations might have a stronger tendency to deviate from the PDB conformations.
For each example, the obtained conformations were compared to the first conformation deposited in the PDB. Minimum RMSD values in the range 1.12.1 Å were obtained for all targets, except 2KSL for which the minimum RMSD value was 3.0 Å. Thus the BranchandPrune algorithm was able to capture conformations close to the PDB conformations, the larger value obtained for 2KSL arising from the larger mechanical strain quoted above.
For each calculation, the conformation displaying the smallest number of NMR constraint violations was compared to the first conformation deposited in the PDB. The RMSD values are smaller than 1.5 Å for 2JMY and 2KXA. This shows that, in the context of the iBP algorithm, the measured NMR constraints also push the structure toward the PDB structure. For 2JMY_1 and 2JMY_2, the RMSD value increases since the definition of the α helical region is shorter. For 2KSL, the conformation displaying the smallest number of constraint violations, displays an RMSD of 3.5Å with the PDB first conformation, which agrees with the maximum number of violations observed for this protein and with the minimum RMSD with the PDB structure analyzed above.
From the PROCHECK [60] analysis, the percentage of residues located in core and allowed Ramachandran regions, is larger than 95% for all targets except 2JMY_1, 2JMY_2, for which the percentages are about 80% due to the reduced definition of the α helix. For all targets, the percentage of residues in disallowed regions is equal to zero. The relatively important percentage of residues located in the allowed region may arise from the systematic exploration performed by the BranchandPrune algorithm, the strict nature of the constraints, and the nature of the pruning devices.
In order to further probe the robustness of the proposed algorithm, iBP calculations on 2KXA and 2KSL have been performed, using input data degraded in the following way: (i) the length of each α helix has been reduced by 1 residues at each extremity, (ii) the lower and upper bounds of the longrange distance constraints have been increased by 0.5 Å. The introduction of this noise into the α helical and longrange constraints makes the iBP solution moving apart from the PDB structure, as the minimum RMSD to PDB structure changes from 1.1 to 2 Å for 2KXA, and from 3.0 to 4.3 Å for 2KSL. Nevertheless, the quality of the Ramachandran diagram remains satisfying, with 93.3% and 95.4% of the residues located in the core and allowed regions of the Ramachandran plot for 2KXA and 2KSL.
In the SOMs for the four calculations (Figure 6), the RMSD values are colored according to their RMSD from the PDB entry, violet color indicating values smaller than the median value of the sampled RMSD value, green color indicating RMSD values larger than this median value. For 2JMY_1, 2KXA and 2KSL, a larger number of neurons of the SOMs belongs to the second group, which is the sign of an enhanced sampling of the conformational space with respect to the region sampled by simulated annealing. For 2JMY_2, the inverse picture is observed, which may arise from the more limited conformational space available to be sampled for a unique αhelix.
Conclusions
We proposed here a BranchandPrune algorithm (iBP) to solve the Distance Geometry Problem, in order to sample exhaustively the conformational space of the backbone of αhelical proteins. The iBP algorithm bears a very slight reminiscence to variable target function approaches for example implemented in DISMAN [67], due to the sequential nature of introducing constraints and nonbonded interactions. However, the precise way of introducing the constraints and nonbonded interactions differs significantly, and DISMAN does not systematically search space but is an optimization approach.
We introduced new pruning devices integrated in the iBP algorithm for DGP with intervals and we tested our iBP implementation on the backbones of αhelical proteins. Several pruning devices have been designed to enforce aminoacid chirality, αhelix geometry and van der Waals steric hindrance. The algorithm allowed to efficiently reconstruct backbone conformations of three αhelical peptides, of various sizes, and for which the structure were previously solved by NMR. The obtained solutions satisfy most of the NMR constraints involving backbone hydrogen bonds, and display very acceptable Ramachandran statistics. The present work represents a first successful step on the way to reconstruct protein structures using a branchandprune algorithm applied to the Distance Geometry problem.
Applications where this approach could have significant advantages are cases where there are few distances defining the tertiary structure of a protein, where it is important to characterize the space of all solutions. It might also be useful as part iterative automated assignment algorithms such as ARIA [68], CYANA [69] or UNIO [70], where in a first iteration all solutions compatible with a few unambiguous longrange constraints could be generated to reduce the ambiguity of the remaining constraints. Another application of the approach proposed here would be to provide input molecular conformations to model the structure of multisubunit complexes into an electron microscopy density map [71].
Some limitations of the current version of iBP prevent for the moment its use with real nuclear Overhauser effect (NOE) data. These limitations are the use of unambiguous distance constraints, the noninclusion of protein sidechains, the loss of information intervals and the appropriate weighting of the various constraints in order to overcome the inconsistencies contained among the whole constraint set. Protein sidechains can be added to the protein backbone afterward. The discretization of circle arcs could be tackled using algebraic geometry and geometric algebra approaches [72]. The Bayesian approach [73] developed for the objective weighting of various NMR contraints according to the data quality could be used to alleviate the inconsistency problems. The use of unambiguous distance constraints is probably the most unavoidable aspect of the current setup of the algorithm.
Declarations
Acknowledgments
TM, MN, BB thank the Institut Pasteur and the CNRS for support. This work was funded by the European Union (FP7IDEASERC 294809 to MN), the “investissement d’avenir” program (grant bip:bip to MN and LL), and the Brazilian Research Agencies FAPESP and CNPq (to CL and RA).
Authors’ Affiliations
References
 Huang X, Britto M, KearScott J, Boone C, Rocca J, Simmerling C, et al. The role of select subtype polymorphisms on HIV1 protease conformational sampling and dynamics. J Biol Chem. 2014; 289:17203–14.View ArticlePubMedPubMed CentralGoogle Scholar
 Kanelis V, FormanKay J, Kay L. Multidimensional NMR methods for protein structure determination. IUBMB Life. 2001; 52:291–302.View ArticlePubMedGoogle Scholar
 Sinz A. Chemical crosslinking and mass spectrometry for mapping threedimensional structures of proteins and protein complexes. J Mass Spectrometry. 2003; 38:1225–37.View ArticleGoogle Scholar
 MartiRenom M, Stuart A, Fiser A, Sánchez R, Melo F, Sali A. Comparative protein structure modeling of genes and genomes. Annu Rev Biophys Biomol Struct. 2000; 29:291–325.View ArticlePubMedGoogle Scholar
 Vajda S, Kozakov D. Convergence and combination of methods in proteinprotein docking. Curr Opin Struct Biol. 2009; 19:164–70.View ArticlePubMedPubMed CentralGoogle Scholar
 Bello M, MartínezArchundia M, CorreaBasurto J. Automated docking for novel drug discovery. Expert Opin Drug Discovery. 2013; 8:821–34.View ArticleGoogle Scholar
 Crippen G, Havel T. Distance geometry and molecular conformation. New York: Wiley; 1988.Google Scholar
 Liberti L, Lavor C, Maculan N, Mucherino A. Euclidean distance geometry and applications. SIAM Rev. 2014; 56:3–69.View ArticleGoogle Scholar
 Nilges M, Gronenborn A, Brünger A, Clore G. Determination of threedimensional structures of proteins by simulated annealing with interproton distance restraints. Application to crambin, potato carboxypeptidase inhibitor and barley serine proteinase inhibitor 2. Protein Eng. 1988; 2:27–38.View ArticlePubMedGoogle Scholar
 Alipanahi B, Krislock N, Ghodsi A, Wolkowicz H, Donaldson L, Li M. Determining protein structures from NOESY distance constraints by semidefinite programming. J Comput Biol. 2013; 20:296–310.View ArticlePubMedPubMed CentralGoogle Scholar
 Wang C, LozanoPérez T, Tidor B. AmbiPack: a systematic algorithm for packing of macromolecular structures with ambiguous distance constraints. Proteins. 1998; 32:26–42.View ArticlePubMedGoogle Scholar
 Potluri S, Yan A, Chou J, Donald B, BaileyKellogg C. Structure determination of symmetric homooligomers by a complete search of symmetry configuration space using nmr restraints and Van Der Waals packing. Proteins. 2006; 65:203–19.View ArticlePubMedGoogle Scholar
 Potluri S, Yan A, Donald B, BaileyKellogg C. A complete algorithm to resolve ambiguity for intersubunit NOE assignment in structure determination of symmetric homooligomers. Protein Sci. 2007; 16:69–81.View ArticlePubMedPubMed CentralGoogle Scholar
 Martin J, Yan A, BaileyKellogg C, Zhou P, Donald B. A geometric arrangement algorithm for structure determination of symmetric protein homooligomers from NOEs and RDCs. J Comput Biol. 2011; 18:1507–23.View ArticlePubMedPubMed CentralGoogle Scholar
 Martin J, Yan A, BaileyKellogg C, Zhou P, Donald B. A graphical method for analyzing distance restraints using residual dipolar couplings for structure determination of symmetric protein homooligomers. Protein Sci. 2011; 20:970–85.View ArticlePubMedPubMed CentralGoogle Scholar
 Reardon P, Sage H, Dennison S, Martin J, Donald B, Alam S, et al. Structure of an HIV1neutralizing antibody target, the lipidbound gp41 envelope membrane proximal region trimer. Proc Nat Acad Sci USA. 2014; 111:1391–6.View ArticlePubMedPubMed CentralGoogle Scholar
 Zeng J, Boyles J, Tripathy C, Wang L, Yan A, Zhou P, et al. Highresolution protein structure determination starting with a global fold calculated from exact solutions to the rdc equations. J Biomol NMR. 2009; 45:265–81.View ArticlePubMedPubMed CentralGoogle Scholar
 Gordon D, Hom G, Mayo S, Pierce N. Exact rotamer optimization for protein design. J Comput Chem. 2003; 24:232–43.View ArticlePubMedGoogle Scholar
 Kingsford C, Chazelle B, Singh M. Solving and analyzing sidechain positioning problems using linear and integer programming. Bioinformatics. 2005; 21:1028–36.View ArticlePubMedGoogle Scholar
 Wang L, Donald B. An efficient and accurate algorithm for assigning nuclear Overhauser effect restraints using a rotamer library ensemble and residual dipolar couplings. The IEEE computational systems bioinformatics conference (CSB). Stanford, CA: The Institute of Electrical and Electronics Engineers, Inc.; 812 Aug 2005, pp. 189–202.Google Scholar
 Wang L, Mettu R, Donald B. A polynomialtime algorithm for de novo protein backbone structure determination from NMR data. J Comput Biol. 2006; 13:1276–88.Google Scholar
 O’Neil R, Lilien R, Donald B, Stroud R, Anderson A. Phylogenetic classification of protozoa based on the structure of the linker domain in the bifunctional enzyme, dihydrofolate reductasethymidylate synthase. J Biol Chem. 2003; 278:52980–7.View ArticlePubMedGoogle Scholar
 Lavor C, Liberti L, Maculan N, Mucherino A. The discretizable molecular distance geometry problem. Comput Optimization App. 2012; 52:115–46.View ArticleGoogle Scholar
 Lavor C, Liberti L, Mucherino A. The interval BranchandPrune Algorithm for the Discretizable Molecular Distance Geometry Problem with Inexact Distances. J Global Optimization. 2013; 56:855–71.View ArticleGoogle Scholar
 Engh RA, Huber R. Accurate bond and angle parameters for xray protein structure refinement. Acta Crystallogr Sect A: Found Crystallogr. 1991; 47(4):392–400.View ArticleGoogle Scholar
 Rocchia W, Alexov E, Honig B. Extending the applicability of the nonlinear poissonboltzmann equation: Multiple dielectric constants and multivalent ions. J Phys Chem B. 2001; 105(28):6507–14.View ArticleGoogle Scholar
 Honig B, Nicholls A. Classical electrostatics in biology and chemistry. Science. 1995 May 26; 268(5214):1144–9.View ArticlePubMedGoogle Scholar
 Liberti L, Masson B, Lee J, Lavor C, Mucherino A. On the number of realizations of certain Henneberg graphs arising in protein conformation. Discrete Appl Mathematics. 2014; 165:213–32.View ArticleGoogle Scholar
 Coope I. Reliable computation of the points of intersection of n spheres in \(\mathbb {R}^{n}\). ANZIAM Journal. 2000; 42:461–77.View ArticleGoogle Scholar
 Berg J, Tymoczko J, Stryer L. Biochemistry: International Edition. New York: WH Freeman & Co; 2006.Google Scholar
 Güntert P, Mumenthaler C, Wüthrich K. Torsion angle dynamics for NMR structure calculation with the new program DYANA. J Mol Biol. 1997; 273:283–98.View ArticlePubMedGoogle Scholar
 Güntert P, Wüthrich K. Sampling of conformation space in torsion angle dynamics calculations. Comp Phys Commun. 2001; 138:155–69.View ArticleGoogle Scholar
 LópezMéndez B, Güntert P. Automated protein structure determination from NMR spectra. J Am Chem Soc. 2006; 128:13112–22.View ArticlePubMedGoogle Scholar
 Mucherino A, Lavor C, Malliavin T, Liberti L, Nilges M, Maculan N. Influence of pruning devices on the solution of molecular distance geometry problems. In: Pardalos, P., Rebennack, S. (eds.) Lecture Notes in Computer Science 6630. Germany: Springer: 2011. p. 206–17.Google Scholar
 Dong Q, Wu Z. A geometric buildup algorithm for solving the molecular distance geometry problem with sparse distance data. J Global Optimization. 2003; 26(3):321–33.View ArticleGoogle Scholar
 Lavor C, Liberti L, Mucherino A, Maculan N. On a discretizable subclass of instances of the molecular distance geometry problem. In: Proceedings of the 2009 ACM Symposium on Applied Computing. ACM Press: 2009. p. 804–5.Google Scholar
 Floyd RW. Algorithm 97: shortest path. Commun ACM. 1962; 5(6):345.View ArticleGoogle Scholar
 Shen Y, Delaglio F, Cornilescu G, Bax A. TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR. 2009; 44:213–23.View ArticlePubMedPubMed CentralGoogle Scholar
 Grishaev A, Bax A. An empirical backbonebackbone hydrogenbonding potential in proteins and its applications to NMR structure refinement and validation. J Am Chem Soc. 2004; 126(23):7281–92.View ArticlePubMedGoogle Scholar
 Kabsch W, Sander C. Dictionary of protein secondary structure: pattern recognition of hydrogenbonded and geometrical features. Biopolymers. 1983; 22(12):2577–637.View ArticlePubMedGoogle Scholar
 Abrahams D, Gurtovoy A. C++ Template Metaprogramming: Concepts, Tools, and Techniques from Boost and Beyond. Boston, Massachusetts: AddisonWesley Professional; 2004.Google Scholar
 Austern MH. Generic Programming and the STL: Using and Extending the C++ Standard Template Library. Boston, Massachusetts: AddisonWesley Longman Publishing Co., Inc.; 1998.Google Scholar
 Josuttis N. The C++ Standard Library: a Tutorial and Reference. Boston, Massachusetts: AddisonWesley Professional; 1999.Google Scholar
 Anderson E, Bai Z, Bischof C, Blackford S, Demmel J, Dongarra J, et al. LAPACK Users’ Guide, 3rd edn. Philadelphia, PA: Society for Industrial and Applied Mathematics; 1999.View ArticleGoogle Scholar
 Lee LQ, Lumsdaine A. The Boost Graph Library: User Guide and Reference Manual. Boston, Massachusetts: AddisonWesley Professional; 2002.Google Scholar
 Brönnimann H, Melquiond G, Pion S. The design of the boost interval arithmetic library. Theor Comput Sci. 2006; 351(1):111–8.View ArticleGoogle Scholar
 Brönnimann H, Melquiond G, Pion S. The boost interval arithmetic library. In: Proceedings of the 5th Conference on Real Numbers and Computers. Lyon, France: 2003. p. 65–80. http://www.lri.fr/~melquion/doc/03rnc5article.ps.gz.
 Saxe J. Embeddability of weighted graphs in kspace is strongly NPhard. Proc 17th Allerton Conference Commun Control Comput. Monticello, Illinois; 1979:4809.Google Scholar
 Liberti L, Lavor C, Maculan N. A branchandprune algorithm for the molecular distance geometry problem. Int Trans Operational Res. 2008; 15:1–7.View ArticleGoogle Scholar
 Lavor C, Mucherino A, Liberti L, Maculan N. On the computation of protein backbones by using artificial backbones of hydrogens. J Global Optimization. 2011; 50:329–44.View ArticleGoogle Scholar
 Costa V, Mucherino A, Lavor C, Cassioli A, Carvalho L, Maculan N. Discretization orders for protein side chains. J Global Optimization. 2014; 60:333–49.View ArticleGoogle Scholar
 Lavor C, Liberti L, Maculan N, Mucherino A. The discretizable molecular distance geometry problem. Comput Optimization App. 2012; 52:115–46.View ArticleGoogle Scholar
 Liberti L, Lavor C, Mucherino A. The discretizable molecular distance geometry problem seems easier on proteins. In: Mucherino A, Lavor C, Liberti L, Maculan N (eds.) Distance Geometry: Theory, Methods, and Applications. New York: Springer: 2013.Google Scholar
 Cassioli A, Günlük O, Lavor C, Liberti L. Discretization vertex orders for distance geometry. Discrete Applied Mathematics (accepted). in press.Google Scholar
 Liberti L, Masson B, Lavor C, Lee J, Mucherino A. On the number of realizations of certain Henneberg graphs arising in protein conformation. Discrete Appl Mathematics. 2014; 165:213–32.View ArticleGoogle Scholar
 Lavor C, Lee J, LeeSt. John A, Liberti L, Mucherino A, Sviridenko M. Discretization orders for distance geometry problems. Optimization Lett. 2012; 6:783–96.View ArticleGoogle Scholar
 Berman H, Kleywegt G, Nakamura H, Markley J. The future of the Protein Data Bank. Biopolymers. 2013; 99:218–22.View ArticlePubMedGoogle Scholar
 Respondek M, Madl T, Göbl C, Golser R, Zangger K. Mapping the orientation of helices in micellebound peptides by paramagnetic relaxation waves. J Am Chem Soc. 2007; 129:5228–34.View ArticlePubMedGoogle Scholar
 Lorieau J, Louis J, Bax A. The complete influenza hemagglutinin fusion domain adopts a tight helical hairpin arrangement at the lipid: water interface. Proc Nat Acad Sci USA. 2010; 107:11341–6.View ArticlePubMedPubMed CentralGoogle Scholar
 Laskowski R, MacArthur M, Moss D, Thornton J. PROCHECK: a program to check the stereochemical quality of protein structure. J Appl Crystallogr. 1993; 26:283–91.View ArticleGoogle Scholar
 Bouvier G, Desdouits N, Ferber M, Blondel A, Nilges M. An automatic tool to analyze and cluster macromolecular conformations based on SelfOrganizing Maps. Bioinformatics. 2015. in press.Google Scholar
 Miri L, Bouvier G, Kettani A, Mikou A, Wakrim L, Nilges M, et al. Stabilization of the integraseDNA complex by Mg2+ ions and prediction of key residues for binding HIV1 integrase inhibitors. Proteins. 2014; 82:466–78.View ArticlePubMedGoogle Scholar
 Bouvier G, DuclertSavatier N, Desdouits N, MezianeCherif D, Blondel A, Courvalin P, et al. Functional motions modulating VanA ligand binding unraveled by selforganizing maps. J Chem Inf Model. 2014; 54:289–301.View ArticlePubMedGoogle Scholar
 Kohonen T. Selforganizing Maps. Heidelberg, Germany: Springer; 2001.View ArticleGoogle Scholar
 Fan H, Mark A. Relative stability of protein structures determined by Xray crystallography or NMR spectroscopy: a molecular dynamics simulation study. Proteins. 2003; 53:111–20.View ArticlePubMedGoogle Scholar
 Nabuurs S, Spronk C, Vuister G, Vriend G. Traditional biomolecular structure determination by NMR spectroscopy allows for major errors. PLoS Computional Biol. 2006; 2:9.View ArticleGoogle Scholar
 Braun W, Gō N. Calculation of Protein Conformations by ProtonProton Distance Constraints: A New Efficient Algorithm. J Mol Biol. 1985; 186:611–26.View ArticlePubMedGoogle Scholar
 Rieping W, Habeck M, Bardiaux B, Bernard A, Malliavin T, Nilges M. ARIA2: automated NOE assignment and data integration in NMR structure calculation. Bioinformatics. 2007; 23:381–2.View ArticlePubMedGoogle Scholar
 Guntert P. Automated NMR structure calculation with CYANA. Methods Mol Biol. 2004; 278:353–78.PubMedGoogle Scholar
 Guerry P, Herrmann T. Comprehensive automation for NMR structure determination of proteins. Methods Mol Biol. 2012; 831:429–51.View ArticlePubMedGoogle Scholar
 Lasker K, Sali A, Wolfson H. Determining macromolecular assembly structures by molecular docking and fitting into a electron density map. Proteins. 2010; 78:3205–11.View ArticlePubMedPubMed CentralGoogle Scholar
 Lavor C, Alves R, Figueiredo W, Petraglia A, Maculan N. Clifford Algebra and the discretizable molecular distance geometry problem. Adv Appl Clifford Algebras. 2015. in press.Google Scholar
 Bernard A, Vranken W, Bardiaux B, Nilges M, Malliavin T. Bayesian estimation of NMR restraint potential and weight: a validation on a representative set of protein structures. Proteins. 2008; 79:1525–37.View ArticleGoogle Scholar
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