- Open Access
caOmicsV: an R package for visualizing multidimensional cancer genomic data
© Zhang et al. 2016
- Received: 18 November 2015
- Accepted: 14 March 2016
- Published: 22 March 2016
Translational genomics research in cancers, e.g., International Cancer Genome Consortium (ICGC) and The Cancer Genome Atlas (TCGA), has generated large multidimensional datasets from high-throughput technologies. Data analysis at multidimensional level will greatly benefit clinical applications of genomic information in diagnosis, prognosis and therapeutics of cancers. To help, tools to effectively visualize integrated multidimensional data are important for understanding and describing the relationship between genomic variations and cancers.
We implemented the R package, caOmicsV, to provide methods under R environment to visualize multidimensional cancer genomic data in two layouts: matrix layout and combined biological network and circular layout. Both layouts support to display sample information, gene expression (e.g., RNA and miRNA), DNA methylation, DNA copy number variations, and summarized data. A set of supplemental functions are included in the caOmicsV package to help users in generation of plot data sets from multiple genomic datasets with given gene names and sample names. Default plot methods for both layouts for easy use are also implemented.
caOmicsV package provides an easy and flexible way to visualize integrated multidimensional cancer genomic data under R environment.
- R package
- Genomic data visualization
- Multidimensional data visualization
Featured with high-throughput technologies, current translational genomic research of cancers often generates multidimensional data such as mRNA/miRNA expression, DNA methylation, exome sequencing, and SNP/DNA copy number variations [1, 2]. Data analysis at multidimensional level will greatly benefit clinical applications of genomic information in diagnosis, prognosis and therapeutics of cancers. To help, tools to effectively visualize integrated multidimensional data are important for understanding and describing the relationship between genomic variation and cancers [3–5].
Visualizing multidimensional genomic data have been implemented in different ways: genomic coordinate based presentation, heatmaps, and networks views . Genomic coordinate based tools such as UCSC genome browser and Integrative Genomics Viewer are powerful in viewing of detailed sequence and various types of variations as well as epigenomic and transcriptomes profiles that tied to genomic loci [6, 7], and CIRCOS and its implementation under different environments [8–10] help in exploring relationships between genomic alterations or positions. One disadvantage of genomic coordinate based tools is the limitations on numbers of samples and genes displayed simultaneous and integration of genomic variations with network/pathway information. In contrast, heatmap and network views can integrate multiple types of genomic variations independent of genomic loci in multiple sample groups at gene set or pathway level and are commonly used in presenting relationship between genomics variations and sample features and relationship between different genomic alterations [11–13].
The R statistical programming environment, an important open source tool used in cancer research community for statistical analysis and visualization of cancer genomic data, has packages which implemented genomic coordinate based views [14–16] and complex heatmap views . To facility the R with more flexible and easy way in presenting multidimensional genomic information, we developed the caOmicsV package for R, to provide a set of graphic functions for visualizing multidimensional genomic data with two different types of layout: matrix layout (bioMatrix) and circular layout on biological network (bioNetCircos).
The caOmicsV package is implemented with R language only and provides two layouts for displaying multidimensional genomic dataset: bioMatrix and bioNetCircos layout. Both layouts support to display sample features, mRNA and miRNA expression, DNA copy number variations (CNV), DNA methylation data, and summarization data. On bioMatrix layout, clinical features of cancer samples are shown with different colored rectangles, gene expression (mRNA and miRNA) data are plotted as heatmap, DNA methylation status are presented as colored rectangle outlines, and DNA copy number variations are displayed as colored points. Besides gene names and sample names, summarized data can also be presented on the layout as text or bars. On bioNetCircos layout, a biological network is built from given gene expression dataset and genes are presented as nodes on the network. Clinical features, mRNA and miRNA expression, DNA methylation, DNA copy number variation, and other summarized data for each sample are displayed in circular layout on each node (gene) as polygons, heatmap, bars, points, or lines. In the center of each node, link lines could be plotted to display the relationship between two samples. Both layouts are using low level plot functions of R graphics package. For bioNetCircos layout, installation of R igraph package is required.
plotBioMatrix(biomatrixPlotDemoData, summaryType = "text")
bioMatrixLegend(heatmapNames = c("RNASeq", "miRNASeq"),
categoryNames = c("Methyl H", "Methyl L"),
binaryNames = c("CN LOSS", "CN Gain"),
heatmapMin = −3, heatmapMax = 3, colorType = "BlueWhiteRed")
dataNames < − c("Tissue Type", "RNASeq", "miRNASeq", "Methylation", "CNV")
bioNetLegend(dataNames, heatmapMin = −3, heatmapMax = 3)
The input data format for both bioMatrix and bioNetCircos layout plot is a list of data matrix and character vectors. A function getESet() was implemented in caOmicsV package to build the input data list, which take s given set of gene names, sample names, and plot data in data frame format as input and returns a list containing all plot data. To help in preparing input dataset for getESet() function, a set of supporting functions are also provided in the package including of methods of extracting subset data from big data set with given gene names and sample names as well as sorting datasets for desired orders required for the plot methods.
caOmicsV package provides sample way to present integrated multidimensional genomic data under R environment with both matrix layout and circus layout on biological network.
Project name: caOmicsV
Project home page: https://www.bioconductor.org/packages/caOmicsV
Operating systems: any operating system supporting R
Programming language: R
Other requirements: working R installation
Any restriction to use by non-academics: none
This work was supported by NCI intramural research funding in Center for Cancer Research, National Cancer Institute, National Institutes of Health, and the package was developed and tested on Helix system at the Center for Information Technology, National Institutes of Health, Bethesda, Maryland, USA. The data used for implementation and demo of caOmcisV package is based upon data generated by the TCGA Research Network: http://cancergenome.nih.gov/.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- The Cancer Genome Atlas (TCGA). http://cancergenome.nih.gov/. Accessed 2 May 2015.
- International Cancer Genome Consortium. https://icgc.org/. Accessed 2 May 2015.
- Nielsen CB, Cantor M, Dubchak I, Gordon D, Wang T. Visualizing genomes: techniques and challenges. Nat Methods. 2010;7:S5–S15.View ArticlePubMedGoogle Scholar
- Schroeder MP, Gonzalez-Perez A, Lopez-Bigas N. Visualizing multidimensional cancer genomics data. Genome Med. 2013;5:9.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang R, Perez-Riverol Y, Hermjakob H, Vizcaíno JA. Open source libraries and frameworks for biological data visualisation: A guide for developers. Proteomics. 2015;15:1356–74.View ArticlePubMedPubMed CentralGoogle Scholar
- The UCSC Cancer Genomics Browser. https://genome-cancer.ucsc.edu. Accessed 2 May 2015.
- Thorvaldsdóttir H, Robinson JT, Mesirov JP. Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration. Brief Bioinform. 2013;14(2):178–92.View ArticlePubMedPubMed CentralGoogle Scholar
- Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R, Horsman D, Jones SJ, Marra MA. Circos: an information aesthetic for comparative genomics. Genome Res. 2009;19:1639–45.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang H, Davis S, Meltzer PS. RCircos: an R package for Circos 2D track plots. BMC Bioinform. 2013;14:244.View ArticleGoogle Scholar
- An J, Lai J, Sajjanhar A, Batra J, Wang C, Nelson CC. J-Circos: an interactive Circos plotte. Bioinformatics. 2015;31:1463–5.View ArticlePubMedGoogle Scholar
- Cerami E, Gao J, Dogrusoz U, Gross BE, Sumer SO, Aksoy BA, Jacobsen A, Byrne CJ, Heuer ML, Larsson E, Antipin Y, Reva B, Goldberg AP, Sander C, Schultz N. The cBio Cancer Genomics Portal: An Open Platform for Exploring Multidimensional Cancer Genomics Data. Cancer Disco. 2012;2:401–4.View ArticleGoogle Scholar
- Vaske CJ, Benz SC, Sanborn JZ, Earl D, Szeto C, Zhu J, Haussler D, Stuart JM. Inference of patient-specific pathway activities from multi-dimensional cancer genomics data using PARADIGM. Bioinformatics. 2010;26:i237–45.View ArticlePubMedPubMed CentralGoogle Scholar
- Wong CK, Vaske CJ, Ng S, Zachary Sanborn J, Ben S, Haussler D, Stuart JM. The UCSC Interaction Browser: multidimensional data views in pathway context. Nucleic Acids Res. 2013;41:W218–24.View ArticlePubMedPubMed CentralGoogle Scholar
- Yin T, Cook D, Lawrence M. ggbio: an R package for extending the grammar of graphics for genomic data. Genome Biology. 2012;13:R77.View ArticlePubMedPubMed CentralGoogle Scholar
- Gu Z, Gu L, Eils R, Schlesner M, Brors B. circlize implements and enhances circular visualization in R. Bioinformatics. 2014;30:2811–2.View ArticlePubMedGoogle Scholar
- Hu Y, Yan C, Hsu CH, Chen QR, Niu K, Komatsoulis GA, Meerzaman D. OmicCircos: A Simple-to-Use R Package for the Circular Visualization of Multidimensional Omics Data. Cancer Inform. 2014;13:13–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Gu Z. ComplexHeatmap: Making Complex Heatmaps. R package version 1.0.0, https://github.com/jokergoo/ComplexHeatmap. Accessed 2 May 2015.