- Open Access
Erratum to: ‘Aro: a machine learning approach to identifying single molecules and estimating classification error in fluorescence microscopy images’
© Wu and Rifkin. 2016
Received: 29 March 2016
Accepted: 29 March 2016
Published: 9 May 2016
The original article was published in BMC Bioinformatics 2015 16:102
Unfortunately, the original version of this article  contained an error which is detailed below.
We had compared Aro to two published methods for identifying smFISH transcripts – threshold-picking  and FISH-quant . The authors of FISH-quant were able to demonstrate that FISH-quant can perform substantially better than we were able to show. A revised fig. 5b (Fig. 1) shows the new FISH-quant results in green. Although it undercounts at high spot numbers compared to manual curation, it is far more reliable than we had shown, and any undercounting could be straightforwardly corrected.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Wu AC-Y, Rifkin SA. Aro: a machine learning approach to identifying single molecules and estimating classification error in fluorescence microscopy images. BMC Bioinformatics. 2015;16:102.View ArticlePubMedPubMed CentralGoogle Scholar
- Raj A, Rifkin SA, Andersen E, van Oudenaarden A: Variability in gene expression underlies incomplete penetrance. Nature. 2010;463:913–918.Google Scholar
- Mueller F, Senecal A, Tantale K, Marie-Nelly H, Ly N, Collin O, Basyuk E, Bertrand E, Darzacq X, Zimmer C: FISH-quant: automatic counting of transcripts in 3D FISH images. Nat Meth. 2013;10:277–278.Google Scholar