 Software
 Open access
 Published:
variancePartition: interpreting drivers of variation in complex gene expression studies
BMC Bioinformatics volume 17, Article number: 483 (2016)
Abstract
Background
As largescale studies of gene expression with multiple sources of biological and technical variation become widely adopted, characterizing these drivers of variation becomes essential to understanding disease biology and regulatory genetics.
Results
We describe a statistical and visualization framework, variancePartition, to prioritize drivers of variation based on a genomewide summary, and identify genes that deviate from the genomewide trend. Using a linear mixed model, variancePartition quantifies variation in each expression trait attributable to differences in disease status, sex, cell or tissue type, ancestry, genetic background, experimental stimulus, or technical variables. Analysis of four largescale transcriptome profiling datasets illustrates that variancePartition recovers striking patterns of biological and technical variation that are reproducible across multiple datasets.
Conclusions
Our open source software, variancePartition, enables rapid interpretation of complex gene expression studies as well as other highthroughput genomics assays. variancePartition is available from Bioconductor: http://bioconductor.org/packages/variancePartition.
Background
Highthroughput genomics assays have revolutionized our understanding of the molecular etiology of human disease, molecular biology of cell lineage and genetic regulation of gene expression. Transcriptome profiling in particular has been widely applied to detect variation in transcript levels attributable to differences in disease state, cell type or regulatory genetics. As transcriptome profiling studies have expanded in size and scope, they have grown increasingly complex and consider multiple sources of biological and technical variation. Recent studies have simultaneously considered multiple dimensions of variation to understand the impact of cell type [1], tissue type [2], brain region [3], experimental stimuli [4], time duration following stimulus [5] or ancestry [1, 4, 6] on the genetic regulation of gene expression. More studies are including a disease axis, for example to characterize the role of regulatory genetics on late onset Alzheimer’s disease in multiple brain regions [7].
The fundamental challenge in the analysis of complex datasets is to quantify and interpret the contribution of multiple sources of variation. Indeed the most pressing questions concern the relationship between these sources of variation. How does cell or tissue type affect the genetic regulation of gene expression, and does it vary by ancestry [1, 2]? What is the relative contribution of experimental stimulus versus regulatory genetics to variation in gene expression [5]? Is technical variability of RNAseq low enough to study regulatory genetics and disease biology, and what are the major drivers of this technical variability [2, 8, 9]? A rich understanding of complex datasets requires answering these questions with both a genomewide summary and genelevel resolution.
Standard computational workflows employ principal components analysis [10] and hierarchical clustering [11] to summarize genomewide expression patterns, and differential expression [12–16] to perform genelevel analyses. Recently, statistical methods that decompose variation in gene expression into the variance attributable to multiple variables in the experimental design have yielded valuable insight into the biological and technical components driving expression variation [8, 17–22]. Moreover, linear mixed models have been widely used in the analysis and interpretation of genomewide association studies [23–28].
The linear mixed model is uniquely suited to interpreting drivers of variation in complex gene expression studies. Yet the lack of a convenient workflow and scalable implementation for analysis and visualization have prevented wider application of this rich statistical framework. Applying this analysis framework to gene expression data currently requires particular expertise in regression modeling, computational statistics, the R programming language and data visualization. Even then, the time required to implement the analysis is often prohibitive.
As gene expression datasets become more complex, the analysis and interpretation of the data is becoming the ratelimiting step. We have developed the variancePartition software and workflow to facilitate rapid analysis and improve interpretation of complex gene expression datasets. The software and workflow enables any analyst to perform a sophisticated analysis and visualize the results in hours using a few lines of R code. variancePartition leverages the power of the linear mixed model [29–31] to jointly quantify the contribution of multiple sources of variation in highthroughput genomics studies. In applications to transcriptome profiling, variancePartition fits a linear mixed model for each gene and partitions the total variance into the fraction attributable to each aspect of the study design, plus the residual variation. Because it is built on the first principles of the linear mixed model, variancePartition has well characterized theoretical properties [29–31] and accurately estimates the variance fractions even for complex experimental designs where the standard ANOVA method is either inaccurate or not applicable. Moreover, variancePartition gives strong interpretations about the drivers of expression variation, and we demonstrate that these findings are reproducible across multiple datasets.
Here we apply variancePartition to four wellcharacterized gene expression studies to demonstrate how the workflow facilitates interpretation of drivers of expression variation in complex study designs with multiple dimensions of variation. We illustrate how variancePartition enables rapid interpretation of the drivers of expression variation in these complex datasets.
Implementation
Overview of the software
The variancePartition R package implements a computational workflow (Fig. 1) that is complementary to standard analyses and provides particular insight into datasets with multiple dimensions of variation. variancePartition provides a userfriendly, parallelized interface for genomewide analysis and publication quality visualizations to examine the results. Because the variance fractions are simple to describe and interpret, variancePartition can give particular insight into how each dimension of variation contributes to transcriptional variability. A typical variancePartition analysis comprises: 1) fitting a linear mixed model to quantify the contribution of each dimension of variation to each gene, 2) visualizing the results, and 3) integrating additional data about each gene to interpret drivers of this variation. The variancePartition workflow requires only a few lines of R code for preprocessing, analysis and visualization and this enables rapid interpretation of complex datasets.
The variancePartition software is implemented in R and is optimized for genomewide analysis of largescale transcriptome profiling datasets. variancePartition uses the packages lme4 [29] foreach [32], iterators [33] and doParallel [34] to efficiently fit a linear mixed model for each gene in parallel on a multicore machine with a small memory footprint. The precision weights from limma/voom [15] are seamlessly incorporated into the analysis workflow. Builtin publication quality visualizations are implemented in ggplot2 [35]. The variancePartition software including extensive documentation is available from http://bioconductor.org/packages/variancePartition and is compatible with Bioconductor ≥v3.2 for R ≥v3.2.
Linear mixed model framework
variancePartition summarizes the contribution of each variable in terms of the fraction of variation explained (FVE). While the concept of FVE is widely applied to univariate regression by reporting the R ^{2} value from a simple linear model, variancePartition extends FVE to applications with complex study designs with multiple variables of interest. The linear mixed model framework of variancePartition allows multiple dimensions of variation to be considered jointly in a single model and accommodates discrete variables with a large number of categories. This analysis has a similar motivation as the standard ANOVA method. Yet the linear mixed model framework has several statistical and practical advantages that make it more accurate and generally applicable to complex study designs with multiple dimensions of variation (Additional file 1).
Each gene is analyzed separately using the linear mixed model [29–31]
where y is the expression of a single gene across all samples, X _{ j } is the matrix of j ^{th} fixed effect with coefficients β _{ j },Z _{ k } is the matrix corresponding to the k ^{th} random effect with coefficients α _{ k } drawn from a normal distribution with variance \(\sigma ^{2}_{\alpha _{k}}\). The noise term, ε, is drawn from a normal distribution with variance \(\sigma ^{2}_{\varepsilon }\). All parameters are estimated with maximum likelihood [29] as simulations under a range of experimental designs indicate that this approach gives the most accurate FVE estimates (Additional file 1: Figures S1–S4).
Variance terms for the fixed effects are computed using the post hoc calculation
The total variance is
so that the fraction of variance explained by the j ^{th} fixed effect is
by the k ^{th} random effect is
and the residual variance is
In the standard application of variancePartition, these fractions sum to 1 and are always positive by definition. Moreover, the fraction of variation is also interpretable in terms of intraclass correlation, a metric used to assess biological and technical reproducibility [31, 36]. Each gene is processed separately so that only visualization and reporting of genomewide summary statistics use the results from multiple genes.
Parameter estimation
The formulation of the linear mixed model is very general and includes as special cases models where only fixed effects or only random effects are used. When only fixed effects are used, this model corresponds to a fixed effects analysis of variance (ANOVA) where parameters can be estimated with ordinary least squares. When random effects are specified, the variance terms can be estimated with maximum likelihood or restricted maximum likelihood (REML) [37]. Since REML does not directly estimate parameters for fixed effects, these coefficients are estimated after the fact by plugging in estimates for the variance components [29].
We focus on the most general case (i.e. mixed models) that includes both fixed and random effects. In this case parameters can be estimated with maximum likelihood. Maximum likelihood estimates are used exclusively in the main text and are the default in the variancePartition software when random effects are specified because this method performs best in simulations.
Relationship to existing methods
The fixed effects ANOVA model has been widely applied for decades to decompose variance into multiple components of variation [38]. Yet this approach is often inadequate to address the questions that are posed by complex gene expression datasets.
The linear mixed model used by variancePartition has three distinct advantages compared to ANOVA. First, by placing a Gaussian prior on variables modeled as random effects, the linear mixed model more accurately estimates the fraction of variance explained. Even as the number of categories in a discrete variable increases, the linear mixed model still produces accurate estimates because the prior shrinks the estimate for each category towards the zero. Conversely, the fixed effects ANOVA is fit with a linear regression model using ordinary least squares. This method is known to suffer from overfitting and overestimates the variance fractions for variables with many categories. These properties are well established [31, 38, 39] and are consistent with our simulation study (Additional file 1).
Second, the linear mixed model can decompose variance into multiple components in situations where the fixed effects ANOVA cannot be applied because the design matrix is degenerate (i.e. singular). This situation is very common for the types of question of relevant to complex gene expression studies. For example, sex and ancestry are invariant properties of an individual, so jointly analyzing variation across these 3 dimensions of variation involves a degenerate design matrix. In cases like these, the linear mixed model can accurately estimate the desired variance fractions (Additional file 1), while ANOVA will fail to estimate any of these values because the parameters are not identifiable. Thus ANOVA is inadequate for the type of analysis we performed here with variancePartition using linear mixed model.
Finally, the linear mixed model can quantify how variation attributable to one aspect of the study design depends on another, such as the case of crossindividual expression variation depending on tissue/cell type. ANOVA does not have this capability.
Interpretation of percent variance explained
The percent variance explained can be interpreted as the intraclass correlation (ICC). Consider the simplest example of the i ^{th} sample from the k ^{th} individual
with only an intercept term, one random effect corresponding to individual, and an error term. In this case ICC corresponds to the correlation between two samples from the same individual. This value is equal to the fraction of variance explained by individual. For example, consider the correlation between samples from the same individual:
The correlation between samples from different individuals is:
This interpretation in terms of fraction of variation explained (FVE) naturally generalizes to multiple variance components [31]. See Additional file 1 for more details.
Variation across individual within subsets of the data
The linear mixed model underlying variancePartition allows the effect of one variable to depend on the value of another variable. Statistically, this is called a varying coefficient model [31]. This analysis examines the expression variation across individuals within multiple cell types, or another subset of the data. A given sample is only from one cell type, so this analysis asks a question about a subset of the data. The data is implicitly divided into subsets based on cell type and variation explained by individual is evaluated within each subset. This subsetting means that the variance fractions no longer sum to 1, but the model still allows ranking of dimensions of variation based on genomewide contribution to variance and enables analysis of genelevel results. See the Additional file 1 for more details.
Modeling measurement error in RNAseq data
Uncertainty in the measurement of RNAseq data can be modeled with observationlevel precision weights that model the relationship between expression magnitude and sampling variance [15]. variancePartition naturally incorporates these precision weights to create a heteroskedastic linear mixed model [29] that can explicitly account from the measurement uncertainty due to the finite count nature of RNAseq data.
Let the precision w _{ i } denote the inverse of the variance of the observation y _{ i } for the i ^{th} observation. The precisions can be used to reweight the samples in a regression to account for the variation in the uncertainty about each observation. Weighting by the precision upweights samples with low measurement error and down weights samples with high measurement error. Denoting the vector of precision weights for a single gene across all samples as w, the model is fit by weighting the residual variance from equation (8)
These weights are estimated using limma/voom [15] in a preprocessing step and are then incorporated into the variancePartition analysis.
Results
Analysis of GEUVADIS RNAseq dataset
Consider 660 RNAseq experiments from the GEUVADIS study [6, 8] of lymphoblastoid cell lines from 462 individuals of 5 ancestries and 2 sexes sequenced across 7 laboratories. For a single gene, the total variance can be partitioned into the contributions of these components of variation plus residual variance:
The contribution of each driver of variation can be interpreted based on the fraction of total variation it explains. Thus the fraction of variance due to variation across individuals is
and the fractions from all components of variation sum to 1.
Applying variancePartition to the GEUVADIS [6, 8] dataset illustrates how the method can decouple biological and technical variation, and further decompose biological variation into multiple components. Expression variation across individuals, ancestries and sexes is biological, variation across the labs where the samples were sequenced comprise technical artifacts, while the residual variation remains uncharacterized. Results from representative genes illustrate how variancePartition identifies genes where the majority of variation in expression is explained by a single variable such as individual or sex, while variation in other genes is driven by multiple variables (Fig. 2 a). Since the variance fractions sum to 1 for each gene, it is simple to compare results across genes and across sources of variation. Visualizing these results genomewide illustrates that variation across individuals is the major source of expression variation and explains a median of 55.1% of variance genomewide (Fig. 2 b). The median variance explained by laboratory (6.8%), ancestry (4.9%) and sex (0%) is substantially smaller. We note that the variance explained by individual increases to 63.8% when ancestry is removed from the analysis since ancestry is a biological property of each individual (Additional file 1: Figure S5).
Yet particular genes show substantial deviation from the genomewide trend. This is particularly noticeable for sex, where of the 51 genes for which sex explains more that 10% variance 46 are on the X or Y chromosomes. For example, variation across sex explains 98% variance in UTY on the Y chromosome (Fig. 2 c). While differential expression measures the differences in mean expression between the sexes, variancePartition measures the variance within and between each sex. This analysis indicates that variation across sexes explains very little variation genomewide, but has a strong effect on a small number of genes.
Integrating additional data with genelevel results from variancePartition can give a clear interpretation of the drivers of variation. For example, 91.1% of variation in CCDC85B is explained by variation across laboratory. This gene has a very high GC content of 70.9% and is consistent with the genomewide pattern where the degree of variation across laboratories is positively correlated with GC content (Fig. 2 d). While technical variation in RNAseq is known to depend on GC content [8, 9], variancePartition gives a clear illustration of how the effect of technical artifacts varies substantially across genes. Moreover, this analysis can be used to identify other correlates underlying technical issues in expression variation.
In addition, variancePartition gives a strong interpretation to genes whose expression varies across individuals by relating the genelevel results to cisregulatory variation. For example, the fact that 90.6% of variation in ZNF470 is explained by individual suggests that this variation is driven by genetics, and, in fact, ZNF470 has a ciseQTL (Fig. 2 e). This observation is also seen genomewide, as genes with a greater fraction of variation across individuals have a significantly higher probability of having a ciseQTL detected in this study (Fig. 2 f). This analysis explicitly demonstrates how expression variation across individuals is driven by cisregulatory variation.
Analysis of SEQC RNAseq dataset
The Sequencing Quality Control (SEQC) project [9] evaluated the technical reproducibility of RNAseq data by sequencing the same 4 RNA samples at 6 laboratories, using 108 total library constructions and up to 8 lanes on each of 11 Illumina HiSeq 2000 flowcells for a total of 1580 RNAseq experiments. The goal of the study was to determine the degree to which these technical factors explain variation in gene expression measurements. This complex dataset has multiple levels of variation and variancePartition provides a rigorous statistical framework to quantify and interpret these sources of variation in a single analysis.
As expected, variation across the 4 RNA samples is the major axis of variation, explaining a median of 87.5% of variation in expression (Fig. 3 a). But the real interest is in the sources of technical variability. The fact that the technical variables laboratory (2.93%), library (2.55%), flowcell (0.0057%), and lane (0.0000000038%) explain a small fraction of the total variation indicate that these RNAseq experiments were highly reproducible genomewide. Interpreting these values in terms of the intraclass correlation indicates that two experiments from the sample RNA sample but which differ in all other aspects of the study design are highly correlated (median 87.5%). Conversely, two experiments from the same lane, but different RNA samples, etc, show negligible correlation as is expected when technical variation is low.
Analysis and visualization with variancePartition succinctly illustrates that while variation across laboratories and library constructions is not negligible, it is small compared with the magnitude of biological variation for the large majority of genes. Moreover, variation across flowcells and lanes is very small in this dataset. Thus variancePartition illustrates that RNAseq data is highly reproducible genomewide with a small subset of genes showing large technical artifacts.
However, there are notable deviations from these genomewide trends. First, there are a set of transcripts that show little variation between the 4 RNA samples and, in fact, these correspond to spikein synthetic RNA added to each sample at a standardized concentration to act as controls having equal abundance in all experiments [40]. As expected, spikein transcripts show significantly less variation across the 4 RNA samples than human genes (Fig. 3 b). Second, although technical effects are low for most genes, a small number of genes show high variation across laboratories and library constructions. In fact, the fraction of variation across laboratories correlates with the GC content of each gene (Fig. 3 c), and recapitulates the known role of GC content with reproducibility of RNAseq data [8, 41–43].
Analysis of ImmVar microarray dataset
The Immune Variation (ImmVar) project assayed gene expression in CD14 ^{+}CD16 ^{−} monocytes and CD4 ^{+} Tcells on the Affymetrix Human Gene 1.0 ST Array platform in order to characterize the role of cell type in genetic regulation of gene expression [1]. Analysis of 398 individuals with data from both cell types reveals that multiple variables contribute to expression variation in this dataset (Fig. 4 a). Since variancePartition reports the contribution of each variable while simultaneously correcting for all other values, it is apparent that the variation across cell types is the strongest biological driver of variation (16.4%) followed by variation across individuals (5.6%). Although cell type has a smaller median effect than batch, it is notable that cell type explains >50% of the variation for 4,591 genes. The observation that batch and cell type are the strongest drivers of variation is largely consistent with results from principal components analysis (PCA) (Fig. 4 b). We note that the relationship between variancePartition and PCA depends on both the fraction of expression variation explained by a particular variable across all genes as well as the dimension of the variable. While variation across the 2 cell types explains less expression variation than variation across the 6 batches, the first principal component separates samples by cell type because this variable spans a lowerdimensional space.
Meanwhile, sex drives expression variation in a small number of genes, while the age of each individual has a negligible effect. We note that despite the large batch effect observed in this dataset, the biological variation across cell type, individual and sex are still large enough to make meaningful conclusions about cellspecific regulatory genetics when this technical effect is accounted for [1].
Moreover, variancePartition identifies genes that vary along different aspects of the study design (Fig. 4 c), and visualization of a subset of these genes illustrates the strong expression differences when stratified by sex, cell type and individual (Fig. 4 d–f). variancePartition enables further interpretation of the batch effect because it gives results at a genelevel resolution. The samples were processed in 6 technical batches and this axis of variation explains a median of 29.4% of total variation, indicating a large technical effect. Consistent with other analyses, the fraction of variation explained by batch at the genelevel is positively correlated with GC content (Fig. 4 g).
By leveraging the flexibility of the linear mixed model, variancePartition can quantify the variation across individuals within each cell type. Since the variance is analyzed within multiple subsets of the data and each sample is only in a single subset, the total variation explained no longer sums to 1 as it does for standard application of variancePartition. Yet the results allow ranking of dimensions of variation based on genomewide contribution to variance and enables analysis of genelevel results (Additional file 1). This analysis uses the fact that 34 individuals within monocytes have at least 1 technical replicate, while 41 individuals within Tcells have at least 1 technical replicate.
The variation across individuals within Tcells (median 33.2%) and monocytes (median 16.4%) is substantially larger than when the two cell types were combined (Fig. 4 h). The fact that the contribution of individual varies between cell types is consistent with cellspecific regulatory genetics [1]. Finally, the fraction of variation explained by individual within each cell type at the genelevel is directly related to the probability of each gene having ciseQTL within the corresponding cell type (Fig. 4 i).
Analysis of GTEx RNAseq dataset
Application of variancePartition to post mortem RNAseq data of multiple tissues tissues from the GTEx Consortium [2] decouples the influence of multiple biological and technical drivers of expression variation. We analyzed 489 experiments from 103 individuals in 4 tissues (blood, blood vessel, skin and adipose tissue) in order to restrict the analysis to tissues with RNAseq data for most individuals (Additional file 1: Table S1). Variation across tissues is the major source of variation (median 37.4%) while the technical variables expression batch (2.9%), ischemic time (1.2%), RNA isolation batch (0.4%), and RIN (0.2%) have a moderate effect on expression variation genomewide (Fig. 5 a). Variation across expression batches is correlated with GC content but to a lesser degree that other datasets (Additional file 1: Figure S6). Cumulatively, these technical variables explain only 4.7% of the total expression variation. Concerns about reliability of RNAseq data from post mortem samples has been raised due to the potential effects of RNA degradation following cell death [44, 45]. variancePartition analysis indicates that variation in ischemic time has as relatively small effect genomewide and the fraction of variance it explains is comparable to technical effects, yet the effect varies substantially across genes.
The flexibility of the linear mixed model framework allows variancePartition to analyze crossindividual variation within each tissue. We note again that since the variance is analyzed within multiple subsets of the data, the total variation explained no longer sums to 1 here. While variation across individuals explains only a median of 2.3% of variation when all tissues types are considered together, there is substantial variation across individuals within each tissue separately (Fig. 5 b). Crossindividual variation is highest in blood (median 60.3%), while skin (36.5%), blood vessel (22.5%), and adipose tissue (17.7%) exhibit lower crossindividual variation. The fraction of variation explained by individual within each tissue is directly related to the probability of each gene having a ciseQTL within the corresponding tissue (Fig. 5 c). This association is not as strong as in other datasets likely due to the smaller number of individuals and to the relatively small fraction of variation across individuals in adipose tissue.
At the genelevel, variancePartition can prioritize genes based on multiple criteria. For examples, GLMP exhibits higher variation across individuals within blood but low variation in skin (Fig. 5 d). This is consistent of a tissuespecific regulatory variation, and, in fact, the ciseQTL rs2296374 influences gene expression in blood but not in skin (Fig. 5 e).
Discussion
As the scope of gene expression studies continues to expand, the need to quantify and interpret multiple drivers of expression variation is becoming essential. Here we present variancePartition, a publicly available software package that leverages the power of the linear mixed model to quantify the contribution of multiple sources of variation in complex gene expression datasets. For each gene, this analysis partitions the total expression variance into the fraction attributable to each aspect of the study design. A variancePartition analysis gives a genomewide summary of the drivers of variation, but also produces genelevel results to identify genes that deviate from the genomewide trend.
The fraction of expression variation is easily interpretable across genes, drivers of variation and datasets. Thus variancePartition produces a more detailed and quantitative genomewide overview than the standard principal components analysis (PCA) [10] and hierarchical clustering (HC) [11] approaches. PCA and HC focus on the major axis of variation, and they overlook the secondary drivers of variation that can be well characterized with variancePartition. Moreover, the genelevel results from variancePartition indicate genes that deviate from the genomewide trend and integration with additional data can enable a further interpretation. While PCA and HC do not give genelevel results, differential expression (DE) analysis reports genelevel fold change and corresponding pvalue for each aspect of the study design. Yet DE analysis does not produce a clear genomewide summary, and the fold change and pvalues are not easily comparable across multiple drivers of variation.
Analysis of publicly available gene expression studies demonstrate that variancePartition recovers striking patterns of biological and technical variation that are reproducible across multiple datasets. At a genomewide level, expression variation across individuals and cell types is large enough to overcome the technical variation of transcriptome profiling. Yet at the genelevel there is substantial deviation from the genomewide trend due to a range of biological and technical factors. By quantifying the variance attributable to each aspect of the study design, variancePartition facilitates the interpretation of these genelevel effects in the context of additional information. We demonstrate reproducible findings that crossindividual variation is driven by ciseQTL’s and technical variation across laboratories associated with GC content. Moreover, variation across individuals and the relationship to ciseQTL’s depend on the cell or tissue type.
Conclusions
The variancePartition workflow and implementation makes the rich linear mixed model framework easily applicable for interpreting drivers of variation in complex gene expression data. variancePartition provides a general statistical and visualization framework for studying drivers of variation in RNAseq datasets in many types of highthroughput genomic assays including RNAseq (gene, exon and isoformlevel quantification, splicing efficiency), protein quantification, metabolite quantification, metagenomic assays, methylation arrays and epigenomic sequencing assays. Although we have focused here on largescale studies, variancePartition analysis has given valuable insight into RNAseq datasets with as few as 20 samples. The variancePartition software is an open source R package and is freely available on Bioconductor. The software can easily be applied to RNAseq quantifications from featureCounts [46], HTSeq [47], kallisto [48], sailfish [49], salmon [50], RSEM [51] and cufflinks [52] which have been processed in R with limma/voom [15], DESeq2 [16], tximport [53] and ballgown [54]. The software provides a userfriendly interface for analysis and visualization with extensive documentation, and will enable routine application to a range of genomics datasets.
Availability of data and materials

Project name: variancePartition

Project home page: http://bioconductor.org/packages/variancePartition

Operating systems: Linux, Mac OS X, Windows

Programming language: R ≥ v3.2

Other requirements: Bioconductor ≥ v3.2

License: GPL2
Abbreviations
 ANOVA:

Analysis of variance
 DE:

Differential expression
 eQTL:

Expression quantitative trait locus
 FVE:

Fraction of variance explained
 HC:

Hierarchical clustering
 ICC:

Intraclass correlation
 IQR:

Interquartile range PCA: Principal components analysis
 REML:

Restricted maximum likelihood
References
Raj T, Rothamel K, Mostafavi S, Ye C, Lee MMN, Replogle JM, Feng T, Asinovski N, Frohlich I, Imboywa S, Von Korff A, Okada Y, Patsopoulos NA, Davis S, McCabe C, Paik HI, Srivastava GP, Raychaudhuri S, Hafler DA, Koller D, Regev A, Hacohen N, Mathis D, Benoist C, Stranger BE, De Jager PL. Polarization of the effects of autoimmune and neurodegenerative risk alleles in leukocytes. Science. 2014; 344(6183):519–23. doi:http://dx.doi.org/10.1126/science.1249547.
GTEx Consortium. The GenotypeTissue Expression (GTEx) pilot analysis: Multitissue gene regulation in humans. Science. 2015; 348(6235):648–60. doi:http://dx.doi.org/10.1126/science.1262110.
Ramasamy A, Trabzuni D, Guelfi S, Varghese V, Smith C, Walker R, De T, Hardy J, Ryten M, Trabzuni D, Guelfi S, Weale ME, Ramasamy A, Forabosco P, Smith C, Walker R, Arepalli S, Cookson MR, Dillman A, Gibbs JR, Hernandez DG, Nalls MA, Singleton AB, Traynor B, van der Brug M, Ferrucci L, Johnson R, Zielke R, Longo DL, Troncoso J, Zonderman A, Coin L, de Silva R, Cookson MR, Singleton AB, Hardy J, Ryten M, Weale ME. Genetic variability in the regulation of gene expression in ten regions of the human brain. Nat Neurosci. 2014; 17(10):1418–28. doi:http://dx.doi.org/10.1038/nn.3801.
Lee MN, Ye C, Villani AC, Raj T, Li W, Eisenhaure TM, Imboywa SH, Chipendo PI, Ran FA, Slowikowski K, Ward LD, Raddassi K, McCabe C, Lee MH, Frohlich IY, Hafler D. a, Kellis M, Raychaudhuri S, Zhang F, Stranger BE, Benoist CO, De Jager PL, Regev A, Hacohen N. Common genetic variants modulate pathogensensing responses in human dendritic cells. Science. 2014; 343(6175):1246980. doi:http://dx.doi.org/10.1126/science.1246980.
Fairfax BP, Humburg P, Makino S, Naranbhai V, Wong D, Lau E, Jostins L, Plant K, Andrews R, McGee C, Knight JC. Innate immune activity conditions the effect of regulatory variants upon monocyte gene expression. Science. 2014; 343(6175):1246949. doi:http://dx.doi.org/10.1126/science.1246949.
Lappalainen T, Sammeth M, Friedländer MR, ‘t Hoen PAC, Monlong J, Rivas MA, GonzàlezPorta M, Kurbatova N, Griebel T, Ferreira PG, Barann M, Wieland T, Greger L, van Iterson M, Almlöf J, Ribeca P, Pulyakhina I, Esser D, Giger T, Tikhonov A, Sultan M, Bertier G, MacArthur DG, Lek M, Lizano E, Buermans HPJ, Padioleau I, Schwarzmayr T, Karlberg O, Ongen H, Kilpinen H, Beltran S, Gut M, Kahlem K, Amstislavskiy V, Stegle O, Pirinen M, Montgomery SB, Donnelly P, McCarthy MI, Flicek P, Strom TM, The Geuvadis Consortium, Lehrach H, Schreiber S, Sudbrak R, Carracedo Á, Antonarakis SE, Häsler R, Syvänen AC, van Ommen GJ, Brazma A, Meitinger T, Rosenstiel P, Guigó R, Gut IG, Estivill X, Dermitzakis ET. Transcriptome and genome sequencing uncovers functional variation in humans. Nature. 2013; 501(7468):506–11. doi:http://dx.doi.org/10.1038/nature12531.
Zhang B, Gaiteri C, Bodea LG, Wang Z, McElwee J, Podtelezhnikov AA, Zhang C, Xie T, Tran L, Dobrin R, Fluder E, Clurman B. Integrated systems approach identifies genetic nodes and networks in lateonset Alzheimer’s disease. Cell. 2013; 153(3):707–20. doi:http://dx.doi.org/10.1016/j.cell.2013.03.030.
’t Hoen P. a. C, Friedländer MR, Almlöf J, Sammeth M, Pulyakhina I, Anvar SY, Laros JFJ, Buermans HPJ, Karlberg O, Brännvall M, den Dunnen JT, van Ommen GJB, Gut IG, Guigó R, Estivill X, Syvänen AC, Dermitzakis ET, Lappalainen T. Reproducibility of highthroughput mRNA and small RNA sequencing across laboratories. Nat Biotechnol. 2013; 31(11):1015–22. doi:http://dx.doi.org/10.1038/nbt.2702.
SEQC/MAQCIII Consortium. A comprehensive assessment of RNAseq accuracy, reproducibility and information content by the Sequencing Quality Control Consortium. Nat Biotechnol. 2014; 32(9):903–14. doi:http://dx.doi.org/10.1038/nbt.2957.
Alter O, Brown PO, Botstein D. Singular value decomposition for genomewide expression data processing and modeling. Proc Natl Acad Sci U S A. 2000; 97(18):10101–6. doi:http://dx.doi.org/10.1073/pnas.97.18.10101.
Eisen MB, Spellman PT, Brown PO, Botstein D. Cluster analysis and display of genomewide expression patterns. Proc Natl Acad Sci U S A. 1998; 95(25):14863–8.
Smyth GK. Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol. 2004; 3(1):3. doi:http://dx.doi.org/10.2202/15446115.1027.
Robinson MD, McCarthy DJ, Smyth GK. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics (Oxford). 2010; 26(1):139–40. doi:http://dx.doi.org/10.1093/bioinformatics/btp616.
Trapnell C, Hendrickson DG, Sauvageau M, Goff L, Rinn JL, Pachter L. Differential analysis of gene regulation at transcript resolution with RNAseq. Nat Biotechnol. 2013; 31(1):46–53. doi:http://dx.doi.org/10.1038/nbt.2450.
Law CW, Chen Y, Shi W, Smyth GK. Voom: precision weights unlock linear model analysis tools for RNAseq read counts. Genome Biol. 2014; 15(2):29. doi:http://dx.doi.org/10.1186/gb2014152r29.
Love MI, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNAseq data with DESeq2. Genome Biol. 2014; 15(12):550. doi:http://dx.doi.org/10.1186/s1305901405508.
Mele M, Ferreira PG, Reverter F, DeLuca DS, Monlong J, Sammeth M, Young TR, Goldmann JM, Pervouchine DD, Sullivan TJ, Johnson R, Segre AV, Djebali S, Niarchou A, Consortium TG, Wright F. a, Lappalainen T, Calvo M, Getz G, Dermitzakis ET, Ardlie KG, Guigo R. The human transcriptome across tissues and individuals. Science. 2015; 348(6235):660–5. doi:http://dx.doi.org/10.1126/science.aaa0355.
Rouhani F, Kumasaka N, de Brito MC, Bradley A, Vallier L, Gaffney D. Genetic background drives transcriptional variation in human induced pluripotent stem cells. PLoS Genet. 2014; 10(6):1004432. doi:http://dx.doi.org/10.1371/journal.pgen.1004432.
Burrows CK, Banovich NE, Pavlovic BJ, Patterson K, Gallego Romero I, Pritchard JK, Gilad Y. Genetic variation, not cell type of origin, underlies the majority of identifiable regulatory differences in iPSCs. PLoS Genet. 2016; 12(1):1005793. doi:http://dx.doi.org/10.1371/journal.pgen.1005793.
Trabzuni D, Thomson PC. Analysis of gene expression data using a linear mixed model/finite mixture model approach: application to regional differences in the human brain. Bioinformatics. 2014; 30(11):1555–61. doi:http://dx.doi.org/10.1093/bioinformatics/btu088.
Listgarten J, Kadie C, Schadt EE, Heckerman D. Correction for hidden confounders in the genetic analysis of gene expression. Proc Natl Acad Sci. 2010; 107(38):16465.
Storey JD, Madeoy J, Strout JL, Wurfel M, Ronald J, Akey JM. GeneExpression Variation Within and Among Human Populations. Am J Hum Genet. 2007; 80(3):502–9. doi:http://dx.doi.org/10.1086/512017.
Yang J, Zaitlen NA, Goddard ME, Visscher PM, Price AL. Advantages and pitfalls in the application of mixedmodel association methods. Nat Genet. 2014; 46(2):100–6. doi:http://dx.doi.org/10.1038/ng.2876.
Yang J, Benyamin B, McEvoy BP, Gordon S, Henders AK, Nyholt DR, Madden P. a, Heath AC, Martin NG, Montgomery GW, Goddard ME, Visscher PM. Common SNPs explain a large proportion of the heritability for human height. Nat Genet. 2010; 42(7):565–9. doi:http://dx.doi.org/10.1038/ng.608.
Zhou X, Stephens M. Genomewide efficient mixedmodel analysis for association studies. Nat Genet. 2012; 44(7):821–4. doi:http://dx.doi.org/10.1038/ng.2310.
Lippert C, Listgarten J, Liu Y, Kadie CM, Davidson RI, Heckerman D. FaST linear mixed models for genomewide association studies. Nat Methods. 2011; 8(10):833–5. doi:http://dx.doi.org/10.1038/nmeth.1681.
Pirinen M, Donnelly P, Spencer CCA. Efficient computation with a linear mixed model on largescale data sets with applications to genetic studies. Ann Appl Stat. 2013; 7(1):369–90. doi:http://dx.doi.org/10.1214/12AOAS586.
Kang HM, Sul JH, Service SK, Zaitlen NA, Kong SY, Freimer NB, Sabatti C, Eskin E. Variance component model to account for sample structure in genomewide association studies. Nat Genet. 2010; 42(4):348–54. doi:http://dx.doi.org/10.1038/ng.548.
Bates D, Mächler M, Bolker B, Walker S. Fitting linear mixedeffects models using lme4. J Stat Softw. 2015; 67(1). doi:http://dx.doi.org/10.18637/jss.v067.i01.
Laird NM, Ware JH. Randomeffects models for longitudinal data. Biometrics. 1982; 38(4):963–74. doi:http://dx.doi.org/10.2307/2529876.
Pinheiro J, Bates D. Mixedeffects models in S and SPlus. New York: Springer; 2000.
Revolution Analytics, Weston S. foreach: Provides Foreach Looping Construct for R. 2015. http://cran.rproject.org/package=foreach.
Revolution Analytics, Weston S. iterators: Provides Iterator Construct for R. 2015. http://cran.rproject.org/package=iterators.
Revolution Analytics, Weston S. doParallel: Foreach Parallel Adaptor for the ’parallel’ Package. 2015. http://cran.rproject.org/package=doParallel.
Wickham H. ggplot2: elegant graphics for data analysis. New York: Springer; 2009.
Nakagawa S, Schielzeth H. Repeatability for Gaussian and nonGaussian data: A practical guide for biologists. Biol Rev. 2010; 85(4):935–56. doi:http://dx.doi.org/10.1111/j.1469185X.2010.00141.x.
Harville DA. Maximum likelihood approaches to variance component estimation and to related problems. J Am Stat Assoc. 1977; 72(358):320–38. doi:http://dx.doi.org/10.2307/2286796.
Gelman A. Analysis of variance – why it is more important than ever. Ann Stat. 2005; 33(1):1–53. doi:http://dx.doi.org/10.1214/009053604000001048.
Wood S. Generalized additive models: an introduction with R. Boca Raton: Chapman & Hall/CRC; 2006.
Munro SA, Lund SP, Pine PS, Binder H, Clevert DA, Conesa A, Dopazo J, Fasold M, Hochreiter S, Hong H, Jafari N, Kreil DP, Labaj PP, Li S, Liao Y, Lin SM, Meehan J, Mason CE, SantoyoLopez J, Setterquist RA, Shi L, Shi W, Smyth GK, StralisPavese N, Su Z, Tong W, Wang C, Wang J, Xu J, Ye Z, Yang Y, Yu Y, Salit M. Assessing technical performance in differential gene expression experiments with external spikein RNA control ratio mixtures. Nat Commun. 2014; 5:5125. doi:http://dx.doi.org/10.1038/ncomms6125.
Li S, Tighe SW, Nicolet CM, Grove D, Levy S, Farmerie W, Viale A, Wright C, Schweitzer PA, Gao Y, Kim D, Boland J, Hicks B, Kim R, Chhangawala S, Jafari N, Raghavachari N, Gandara J, GarciaReyero N, Hendrickson C, Roberson D, Rosenfeld J, Smith T, Underwood JG, Wang M, Zumbo P, Baldwin DA, Grills GS, Mason CE. Multiplatform assessment of transcriptome profiling using RNAseq in the ABRF nextgeneration sequencing study. Nat Biotechnol. 2014; 32(9). doi:http://dx.doi.org/10.1038/nbt.2972.
Li S, Labaj PP, Zumbo P, Sykacek P, Shi W, Shi L, Phan J, Wu PY, Wang M, Wang C, ThierryMieg D, ThierryMieg J, Kreil DP, Mason CE. Detecting and correcting systematic variation in largescale RNA sequencing data. Nat Biotechnol. 2014; 32(9):888–95. doi:http://dx.doi.org/10.1038/nbt.3000.
Risso D, Schwartz K, Sherlock G, Dudoit S. GCcontent normalization for RNASeq data. BMC Bioinformatics. 2011; 12(1):480. doi:http://dx.doi.org/10.1186/1471210512480.
Feng H, Zhang X, Zhang C. mRIN for direct assessment of genomewide and genespecific mRNA integrity from largescale RNAsequencing data. Nat Commun. 2015; 6:7816. doi:http://dx.doi.org/10.1038/ncomms8816.
Gallego Romero I, Pai AA, Tung J, Gilad Y. RNAseq: impact of RNA degradation on transcript quantification. BMC Biol. 2014; 12(1):42. doi:http://dx.doi.org/10.1186/174170071242.
Liao Y, Smyth GK, Shi W. FeatureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics. 2014; 30(7):923–30. doi:http://dx.doi.org/10.1093/bioinformatics/btt656. http://arxiv.org/abs/arXiv:1305.3347v2.
Anders S, Pyl PT, Huber W. HTSeqA Python framework to work with highthroughput sequencing data. Bioinformatics. 2015; 31(2):166–9. doi:http://dx.doi.org/10.1093/bioinformatics/btu638.
Bray NL, Pimentel H, Melsted P, Pachter L. Nearoptimal probabilistic RNAseq quantification. Nat Biotechnol. 2016; 34(5):525–7. doi:http://dx.doi.org/10.1038/nbt.3519. http://arxiv.org/abs/1505.02710.
Patro R, Mount SM, Kingsford C. Sailfish enables alignmentfree isoform quantification from RNAseq reads using lightweight algorithms. Nat Biotechnol. 2014; 32(5):462–4. doi:http://dx.doi.org/10.1038/nbt.2862. http://arxiv.org/abs/1308.3700.
Patro R, Duggal G, Love MI, Irizarry RA, Kingsford C. Salmon provides accurate, fast, and biasaware transcript expression estimates using dualphase inference. bioRxiv. 2016. doi:http://dx.doi.org/10.1101/021592.
Li B, Dewey CN. RSEM: accurate transcript quantification from RNASeq data with or without a reference genome. BMC Bioinformatics. 2011; 12(1):323. doi:http://dx.doi.org/10.1186/1471210512323.
Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, van Baren MJ, Salzberg SL, Wold BJ, Pachter L. Transcript assembly and quantification by RNASeq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010; 28(5):511–5. doi:http://dx.doi.org/10.1038/nbt.1621.
Soneson C, Love MI, Robinson MD. Differential analyses for RNAseq: transcriptlevel estimates improve genelevel inferences. F1000Research. 2016; 4(0):1521. doi:http://dx.doi.org/10.12688/f1000research.7563.2.
Frazee AC, Pertea G, Jaffe AE, Langmead B, Salzberg SL, Leek JT. Ballgown bridges the gap between transcriptome assembly and expression analysis. Nat Biotechnol. 2015; 33(3):243–6. doi:http://dx.doi.org/10.1038/nbt.3172.
Acknowledgements
We would like to thank B. Kidd, B. Losic, N. Beckmann, R. Kosoy and many other colleagues at Mount Sinai and Stanford for providing valuable feedback on the software and manuscript.
Funding
This work was supported by NIH/NHLBI U01 HL10738804 and NIH/NIA U01 HL10738804 to E.E.S. This work was supported in part through the computational resources and staff expertise provided by Scientific Computing at the Icahn School of Medicine at Mount Sinai.
Authors’ contributions
GEH conceived the statistical method, implemented the software and performed the analysis. EES supervised the work. GEH and EES interpreted the results and wrote the manuscript. Both authors read and approved the final manuscript.
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The authors declare that they have no competing interests.
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Supplementary Note. Additional details describing statistical methods and software. Includes results from simulation study and additional figures from data analysis. (PDF 727 kb)
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Hoffman, G.E., Schadt, E.E. variancePartition: interpreting drivers of variation in complex gene expression studies. BMC Bioinformatics 17, 483 (2016). https://doi.org/10.1186/s128590161323z
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DOI: https://doi.org/10.1186/s128590161323z