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Table 1 Hypothetical data used to generate Figure 4a where \( {\Delta C}_q^{(w)} \) is the dependent variable. Calculation of predicted relative expression, \( \hat{R} \), values follows \( {10}^{m\left({x}_0-x\right)} \), where m =  − 0.139, and these values are plotted in Figure 4c. x0 = 9.85 is the mean x. The 95% confidence interval for the slope m is (−0.162, −0.117)

From: Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

α1 concentration (pg/mL) \( {\Delta C}_q^{(w)} \)   α1 concentration (pg/mL) \( \hat{R} \) Lower
Confidence Interval
Upper Confidence Interval
8.7 0.633   8.7 0.692 0.651 0.734
8.9 0.568   8.9 0.738 0.702 0.774
9.4 0.534   9.1 0.787 0.756 0.817
9.5 0.474   9.3 0.839 0.815 0.862
9.8 0.487   9.5 0.894 0.878 0.910
10.1 0.428   9.7 0.953 0.946 0.960
10.2 0.387   9.9 1.016 1.014 1.019
10.4 0.398   10.1 1.083 1.070 1.098
10.6 0.360   10.3 1.155 1.129 1.183
10.9 0.296   10.5 1.231 1.191 1.274
    10.7 1.313 1.257 1.373
    10.9 1.399 1.327 1.479