Table 1 Hypothetical data used to generate Figure 4a where \( {\Delta C}_q^{(w)} \) is the dependent variable. Calculation of predicted relative expression, \( \hat{R} \), values follows \( {10}^{m\left({x}_0-x\right)} \), where m =  − 0.139, and these values are plotted in Figure 4c. x₀ = 9.85 is the mean x. The 95% confidence interval for the slope m is (−0.162, −0.117)

From: Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation