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Table 4 Hypothetical data used to generate Fig. 5a. Calculation of predicted huddling time, \( \hat{y} \), values follows \( \hat{y_0}-m\log (R) \), where m =  − 6.907, and these values are plotted in Fig. 5b. x0 = 0.457 is the mean x, and \( \hat{y_0}=9.847 \). The 95% confidence interval for the slope m is (−8.011, −5.803)

From: Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

\( {\Delta C}_q^{(w)} \) Time spent huddling (min.) \( {\Delta \Delta C}_q^{(w)} \) R \( \hat{y} \) Lower Confidence Interval Upper Confidence Interval
0.633 8.7 0.177 0.666 8.628 8.433 8.823
0.568 8.9 0.112 0.774 9.077 8.954 9.200
0.534 9.4 0.0775 0.837 9.312 9.226 9.397
0.474 9.5 0.0175 0.961 9.726 9.707 9.745
0.487 9.8 0.0305 0.932 9.636 9.603 9.670
0.428 10.1 −0.0285 1.068 10.044 10.012 10.075
0.387 10.2 −0.0695 1.174 10.327 10.250 10.404
0.398 10.4 −0.0585 1.144 10.251 10.187 10.316
0.360 10.6 −0.0965 1.249 10.514 10.407 10.620
0.296 10.9 −0.1605 1.447 10.956 10.778 11.133