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Table 4 Hypothetical data used to generate Fig. 5a. Calculation of predicted huddling time, \( \hat{y} \), values follows \( \hat{y_0}-m\log (R) \), where m =  − 6.907, and these values are plotted in Fig. 5b. x0 = 0.457 is the mean x, and \( \hat{y_0}=9.847 \). The 95% confidence interval for the slope m is (−8.011, −5.803)

From: Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

\( {\Delta C}_q^{(w)} \)

Time spent huddling (min.)

\( {\Delta \Delta C}_q^{(w)} \)

R

\( \hat{y} \)

Lower Confidence Interval

Upper Confidence Interval

0.633

8.7

0.177

0.666

8.628

8.433

8.823

0.568

8.9

0.112

0.774

9.077

8.954

9.200

0.534

9.4

0.0775

0.837

9.312

9.226

9.397

0.474

9.5

0.0175

0.961

9.726

9.707

9.745

0.487

9.8

0.0305

0.932

9.636

9.603

9.670

0.428

10.1

−0.0285

1.068

10.044

10.012

10.075

0.387

10.2

−0.0695

1.174

10.327

10.250

10.404

0.398

10.4

−0.0585

1.144

10.251

10.187

10.316

0.360

10.6

−0.0965

1.249

10.514

10.407

10.620

0.296

10.9

−0.1605

1.447

10.956

10.778

11.133