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Table 6 Hypothetical data used to generate Figure 7A, B. Calculation of predicted relative expression, \( \hat{R} \), values follows \( {\left(\frac{x_0}{x}\right)}^m \), where m =  − 0.116, and these values are plotted in Figure 7C. x0 = 139.8 is the mean x. The 95% confidence interval for the slope m is (−0.123, −0.109)

From: Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

Cells / nL log(cells/nL) \( {\Delta C}_q^{(w)} \) \( \hat{R} \) Lower Confidence Interval Upper Confidence Interval
385.61 2.586 0.345 1.125 1.117 1.133
260.29 2.415 0.368 1.075 1.070 1.079
176.41 2.247 0.381 1.027 1.026 1.029
115.56 2.063 0.405 0.978 0.977 0.979
74.72 1.873 0.433 0.930 0.926 0.934
49.29 1.693 0.445 0.886 0.880 0.893
33.37 1.523 0.468 0.847 0.838 0.855
23.12 1.364 0.488 0.812 0.801 0.822