Table 6 Hypothetical data used to generate Figure 7A, B. Calculation of predicted relative expression, \( \hat{R} \), values follows \( {\left(\frac{x_0}{x}\right)}^m \), where m =  − 0.116, and these values are plotted in Figure 7C. x₀ = 139.8 is the mean x. The 95% confidence interval for the slope m is (−0.123, −0.109)

From: Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation