Skip to main content

Bioinformatics research at SBB-2019

This Special Issue of BMC Bioinformatics “Systems Biology and Bioinformatics” collates the papers presented at the 11th Young Scientists School “Systems Biology and Bioinformatics”-2019 (SBB-2019) hold in summer 2019 in Novosibirsk, Russia ( The issue contains material on classical sequence analysis, bioinformatics applications in medicine, and the theoretical research on gene network structure and dynamics. This traditional school on bioinformatics is organized annually since 2008 by the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences and Novosibirsk State University. In 2019 we had several large international meetings in Russia - in Novosibirsk and Moscow presenting materials on genetics and bioinformatics. The name of the special issue - SBB-2019 reflects the main topics of the event. Traditionally we select the best conference and school materials to be presented at BMC Genomics ( and BMC Genetics post-conference publications [1,2,3,4], see also (

The SBB Schools in Novosibirsk are satellite events for BGRS\SB multiconference organized biannually. In 2019, the SBB-2019 School was held as a broad-scope independent meeting with science and education components. Other Special Issues in the fields of genomics, bioinformatics, microbiology, and medical genomics accompany this Special Issue in bioinformatics, published as a part of the following series: BMC Genomics and BMC Medical Genomics, BMC Genetics and BMC Medical Genetics, as well as in BMC Microbiology. In 2018, the conference highlights were organized into the Special Issues with reports from the BGRS\SB-2018 conference and earlier from Belyaev Readings-2017 ( [5, 6]. We continued the BMC Bioinformatics special issues in 2019 [7, 8]. At the time of this paper writing, the BGRS\SB-2020 event passed in Novosibirsk ( For the first time, it was in an online format. We believe such events and public discussion at the platforms of international publishers bring attention of the readers [8, 9] (;

The papers comprising this issue of BMC Bioinformatics were discussed at the SBB-2019 School in Novosibirsk. We open up this Special Issue by classical work on protein sequence analysis by Valery Polyanovsky and colleagues [10] (this issue). The quality of sequence alignment is determined by the substitution matrix and parameters of the insertion-deletion penalty function. The authors conducted a numerical experiment using a representative sample of existing matrices of various types and origins, such as the classic evolutionary matrix series (PAM, Blosum), structural alignment based matrices, and contact energy matrix. Hence, they made an optimal choice of the substitution matrix and the penalty parameters. The best alignment quality is achieved with matrices corresponding to the most substantial evolutionary distance: Gonnet, VTML250, PAM250, MIQS, and Pfasum050. The same property is inherent in matrices not only of evolutionary origin but also of another background corresponding to a significant evolutionary distance. Therefore, matrices based on structural data show alignment quality close enough to its value for evolutionary matrices — this strategy agrees with the idea that the spatial structure is more conservative than the protein sequence. The study by Anastasia Anashkina et al. [11] (this issue) continues the protein structure analysis topic. The authors consider S-glutathionylation - the formation of disulfide bonds between the tripeptide glutathione and cysteine residues of the protein, protecting them from irreversible oxidation [12]. Based on the heptapeptide sequences, a position-specific matrix was created by analyzing the protein sequence near the cysteine residue. The authors proposed an effective method for calculating the glutathionylation propensity score, which utilizes the position-specific matrix and a criterion for predicting glutathionylated peptides.

The review by Mila Efimenko and colleagues [13] (this issue) is in the medical bioinformatics field. The authors discuss medical image recognition technologies to detect melanomas using neural networks. They searched the PubMed database for systematic reviews and original research papers. The authors considered convolutional and deep-learning neural networks as well as the fuzzy clustering or World Cup Optimization algorithms in analyzing dermatoscopic images. They have shown that neural networks show higher specificity, accuracy, and sensitivity than dermatologists to evaluate the disease features.

Timofey Ivanisenko and co-authors [14] (this issue) present a new module of ANDSystem (Associative Network Discovery System) for the search of knowledge in the scientific literature. This application extends the functionality of the popular ANDSystem tool for automatic text mining of scientific publications [15, 16]. Currently, there are several commercial automated services allowing users to reconstruct molecular-genetic networks using the data automatically extracted from the texts of scientific publications, such as STRING, Pathway Commons, MetaCore, and Ingenuity. The presented ANDDigest system is a new web-based module of the ANDSystem, permitting searching within PubMed by using dictionaries from the ANDSystem tool and sets of user-defined keywords. The popular search engines, such as Google Scholar, PubMed, and Scopus, are powerful universal tools for keyword-based document searches without linking to any specific field of knowledge. Text-mining of scientific publications in bioinformatics by the proposed system is an alternative to such lookup, providing automated extraction and formalized representation of accurate biomedical information [17, 18].

Dr. Likhoshvai co-authored two manuscripts on theoretical investigations of gene networks. Sadly, Dr. Likhoshvai has recently passed away, leaving several unpublished works to be completed by his colleagues. The publications were prepared by Vladimir P. Golubyatnikov [19], and Tamara M. Khlebodarova [20] (this issue). They discuss the dynamic behavior of gene networks and oscillating models. The regulatory feedback loops that present in the structural and functional organization of molecular-genetic systems and the phenomenon of the regulatory signal delay, a period between the moment of signal reception and its implementation, provide natural conditions for complicated dynamic regimes in these systems. In [19], the authors studied the dynamical properties of models of simplest circular gene networks regulated by negative feedback mechanisms. They have demonstrated the existence and stability of oscillating trajectories (cycles) in these models. From the evolutionary viewpoint, the configuration of loops with negative feedback regulation is a more favorable mode for cell functioning. It can be accomplished by a simple blockage of the transcription initiation site by the regulatory protein without the formation of complex structures that interact with the RNA polymerase. This type of regulation is widespread, and its simplest example is a sub-system containing just one gene, which controls its expression by a negative feedback mechanism, representing the minimal regulatory circuit, acting on itself. The system of differential equations presents the simplest model of artificial molecular triggers [21].

We note previous publications on gene network models by the authors, presented at “Systems biology and bioinformatics” Schools in Novosibirsk, Russia [22, 23], the publication on chaos in gene expression dynamics [24,25,26]. The previous works by V.A.Likhoshvai were presented at Systems Biology and Bioinformatics (SBB) Schools since 2012 [24] and discussed in [27, 28].

Tamara Khlebodarova and colleagues applied gene network models for the analysis of dynamic regulation of synaptic protein that may cause neuropsychiatric diseases [20] (this issue). Fragile X Mental Retardation Protein, which regulates the efficiency of dendritic mRNA translation in response to the stimulation of metabotropic glutamate receptors at excitatory synapses of the hippocampal pyramidal cells [29]. Its activity is regulated via positive and negative regulatory loops that function in different time ranges, which is an absolute factor for the formation of chaotic regimes that lead to disrupted proteome stability. A mathematical model that describes the maintenance of a specific pool of active receptors on the postsynaptic membrane via two mechanisms – de novo synthesis of receptor proteins and restoration of protein function during the recycling process – has been developed. A similar analysis of the dynamic behavior of gene networks was applied to local translation at activated synapses, also related to mental disorders [30]. The results on gene model simulation suggested that the chaotic behavior of the network parameters is quite common [31,32,33]. Note the publication by Dr. Likhoshvai on this topic in BioMed Central journals by the SBB School materials in 2014 (; The original work on the dynamic of cell replications [33] continued in recent work [34] in 2019. His work on gene network modeling served as background on current applications by his disciples in databases [26, 35], plant science models [36].

Therefore, this issue includes reports of recent bioinformatics applications in protein sequence and structure analysis, text mining, gene networks modeling. We aim to support international exchange and education in new forms via the schools and competitions for young scientists ( Next, SBB-2020 School in Russia is scheduled for September 2020. BGRS\SB-2020 (Bioinformatics of Genome Regulation and Structure \ Systems Biology) multiconference just over in July 2020 holding series of symposia and workshops (, and following journal publications (; We invite our readers worldwide to attend our next events on Systems Biology and Bioinformatics in Russia, as well as the systems biology meetings in Moscow - Digital Medicine Forum (


  1. Orlov YL, Kolchanov NA, Hofestädt R, Wong L. Editorial: bioinformatics development at the BGRS\SB conference series: 10th anniversary. J Bioinforma Comput Biol. 2017;15(2):1702001.

    Article  Google Scholar 

  2. Baranova AV, Orlov YL. The papers presented at 7th young scientists school “systems biology and bioinformatics” (SBB’15): introductory note. BMC Genet. 2016;17(Suppl 1):20.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Orlov YL, Baranova AV, Markel AL. Computational models in genetics at BGRS\SB-2016: introductory note. BMC Genet. 2016;17(Suppl 3):155.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Orlov YL, Baranova AV, Hofestaedt R, Kolchanov NA. Computational genomics at BGRS\SB-2016: introductory note. BMC Genomics. 2016;17(Suppl 14):996.

    Article  PubMed  PubMed Central  Google Scholar 

  5. Orlov YL, Baranova AV, Herbeck YE. Evolutionary biology at Belyaev conference - 2017. BMC Evol Biol. 2017;17(Suppl 2):260.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Shumny VK. To the centenary of the birth of outstanding evolutionist Dmitri Konstantinovich Belyaev. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov. J Genet Breed. 2017;21(4):387–91. (In Russian).

    Article  Google Scholar 

  7. Tatarinova TV, Chen M, Orlov YL. Bioinformatics research at BGRS-2018. BMC Bioinformatics. 2019;20(Suppl 1):33.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Orlov YL, Baranova AV. Editorial: bioinformatics of genome regulation and systems biology. Front Genet. 2020;11:625.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Orlov YL, Galieva ER, Melerzanov AV. Computer genomics research at the bioinformatics conference series in Novosibirsk. BMC Genomics. 2019;20(Suppl 7):537.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Polyanovsky V, Lifanov A, Esipova NG, Tumanyan VG. The ranging of amino acids substitution matrices of various types in accordance with the alignment accuracy criterion. BMC Bioinformatics. 2020;21(Suppl 11):S2.

  11. Anashkina AA, Poluektov YM, Dmitriev VA, Kuznetsov EN, Mitkevich VA, Makarov AA, Petrushanko IY. A novel approach for predicting protein S-glutathionylation. BMC Bioinformatics. 2020;21(Suppl 11):S3.

  12. Mitkevich VA, Petrushanko IY, Poluektov YM, Burnysheva KM, Lakunina VA, Anashkina AA, Makarov AA. Basal Glutathionylation of Na,K-ATPase α-Subunit Depends on Redox Status of Cells during the Enzyme Biosynthesis. Oxidative Med Cell Longev. 2016;2016:9092328.

    Article  CAS  Google Scholar 

  13. Efimenko M, Ignatev A, Koshechkin K. Review of medical image recognition technologies to detect melanomas using neural networks. BMC Bioinformatics. 2020;21(Suppl 11):S4.

  14. Ivanisenko TV, Saik OV, Demenkov PS, Ivanisenko NV, Savostianov AN, Ivanisenko VA. ANDDigest: a new web-based module of ANDSystem for the search of knowledge in the scientific literature. BMC Bioinformatics. 2020;21(Suppl 11):S5.

  15. Ivanisenko VA, Demenkov PS, Ivanisenko TV, Mishchenko EL, Saik OV. A new version of the ANDSystem tool for automatic extraction of knowledge from scientific publications with expanded functionality for reconstruction of associative gene networks by considering tissue-specific gene expression. BMC Bioinformatics. 2018;20(Suppl 1):34.

    Article  Google Scholar 

  16. Demenkov PS, Ivanisenko TV, Kolchanov NA, Ivanisenko VA. ANDVisio: a new tool for graphic visualization and analysis of literature mined associative gene networks in the ANDSystem. In Silico Biology. 2011-2012;11(3–4):149–61.

    Article  PubMed  CAS  Google Scholar 

  17. Ivanisenko VA, Saik OV, Ivanisenko NV, Tiys ES, Ivanisenko TV, Demenkov PS, Kolchanov NA. ANDSystem: an Associative Network Discovery System for automated literature mining in the field of biology. BMC Syst Biol. 2015;9(Suppl 2):S2.

    Article  PubMed  PubMed Central  Google Scholar 

  18. Saik OV, Nimaev VV, Usmonov DB, Demenkov PS, Ivanisenko TV, Lavrik IN, Ivanisenko VA. Prioritization of genes involved in endothelial cell apoptosis by their implication in lymphedema using an analysis of associative gene networks with ANDSystem. BMC Med Genet. 2019;12(Suppl 2):47.

    Article  Google Scholar 

  19. Likhoshvai VA, Golubyatnikov VP, Khlebodarova TM. Limit cycles in models of circular gene networks regulated by negative feedback loops. BMC Bioinformatics. 2020;21(Suppl 11):S6.

  20. Khlebodarova TM, Kogai VV, Likhoshvai VA. On the dynamic aspects of local translation at the activated synapse. BMC Bioinformatics. 2020;21(Suppl 11):S7.

  21. Tchuraev RN, Stupak IV, Tropynina TS, Stupak EE. Epigenes: design and con-struction of new hereditary units. FEBS Lett. 2000;486(3):200–2.

    Article  PubMed  CAS  Google Scholar 

  22. Khlebodarova TM. Multiple scenarios of transition to chaos in the alternative splicing model. Int J Bifurcat Chaos. 2017;27:1730006.

    Article  Google Scholar 

  23. Likhoshvai VA, Kogai VV, Fadeev SI, Khlebodarova TM. Chaos and hyperchaos in a model of ribosome autocatalytic synthesis. Sci Rep. 2016;6:38870.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  24. Likhoshvai VA, Fadeev SI, Kogai VV, Khlebodarova TM. On the chaos in gene networks. J Bioinforma Comput Biol. 2013;11(1):1340009.

    Article  Google Scholar 

  25. Khlebodarova TM, Kogai VV, Fadeev SI, Likhoshvai VA. Chaos and hyperchaos in simple gene network with negative feedback and time delays. J Bioinforma Comput Biol. 2017;15(2):1650042.

    Article  CAS  Google Scholar 

  26. Kazantsev F, Akberdin I, Lashin S, Ree N, Timonov V, Ratushny A, Khlebodarova T, Likhoshvai V. MAMMOTh: a new database for curated mathematical models of biomolecular systems. J Bioinforma Comput Biol. 2018;16(1):1740010.

    Article  CAS  Google Scholar 

  27. Kolchanov NA, Orlov YL. Introductory note for BGRS-2012 special issue. J Bioinforma Comput Biol. 2013;11(1):1302001.

    Article  Google Scholar 

  28. Orlov YL, Hofestaedt RM, Kolchanov NA. Introductory note for BGRS\SB-2014 special issue. J Bioinforma Comput Biol. 2015;13(1):1502001.

    Article  Google Scholar 

  29. Klein ME, Monday H, Jordan BA. Proteostasis and RNA binding proteins in synaptic plasticity and in the pathogenesis of neuropsychiatric disorders. Neural Plast. 2016;2016:3857934.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  30. Khlebodarova TM, Kogai VV, Trifonova EA, Likhoshvai VA. Dynamic landscape of the local translation at activated synapses. Mol Psychiatry. 2018;23(1):107–14.

    Article  PubMed  CAS  Google Scholar 

  31. Khlebodarova TM, Ree NA, Likhoshvai VA. On the control mechanisms of the nitrite level in Escherichia coli cells: the mathematical model. BMC Microbiol. 2016;16(Suppl 1):7.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  32. Likhoshvai VA, Khlebodarova TM, Bazhan SI, Gainova IA, Chereshnev VA, Bocharov GA. Mathematical model of the tat-rev regulation of HIV-1 replication in an activated cell predicts the existence of oscillatory dynamics in the synthesis of viral components. BMC Genomics. 2014;15(Suppl 12):S1.

    Article  PubMed  PubMed Central  Google Scholar 

  33. Khlebodarova TM, Likhoshvai VA. Persister cells - a plausible outcome of neutral Coevolutionary drift. Sci Rep. 2018;8(1):14309.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  34. Khlebodarova TM, Likhoshvai VA. Molecular Mechanisms of Non-Inherited Antibiotic Tolerance in Bacteria and Archaea. Mol Biol (Mosk). 2019;53(4):531–40. (In Russian).

    Article  CAS  Google Scholar 

  35. Kazantsev FV, Skolotneva ES, Kelbin VN, Salina EA, Lashin SA. MIGREW: database on molecular identification of genes for resistance in wheat. BMC Bioinformatics. 2019;20(Suppl 1):36.

    Article  PubMed  PubMed Central  Google Scholar 

  36. Likhoshvai VA, Kogai VV, Fadeev SI, Khlebodarova TM. Alternative splicing can lead to chaos. J Bioinforma Comput Biol. 2015;13(1):1540003.

    Article  Google Scholar 

Download references


We are grateful to Professor Academician N.A. Kolchanov, Prof. A.V. Kochetov for the organization of the “Systems Biology and Bioinformatics”-2019 event and providing platform for international bioinformatics and genomics research. We acknowledge Prof. A.V. Baranova for the editorial work on SBB-2019 issues and Prof. O.P. Balanovsky for the organization of the “Centenary of Human Population Genetics” conference in Moscow in 2019 and compiling the materials for the SBB-2019 special issues at BioMed Central journals. Thank Prof. G.S. Lebedev for organizarion of the Didital medicine forum at Sechenov University, Moscow.

Special thanks to Dr. T.M. Khlebodarova for restoring the draft manuscripts by late V.A. Likhoshvai presented in this issue.

The holding of the SBB-2019 Young Scientists School in 2019 on the base of the Novosibirsk State University was supported by RFBR (grant 19-04-20036).

The reported studies were supported by the Russian Science Foundation (grant 19-15-00219) (Recipient E.G., genetics models). The publication has been prepared with the support of the RUDN University Program 5-100 (Recipient Y.O., bioinformatics program).

The guest editors of the special issue are grateful to the conference committee members and the reviewers who helped in the peer-review and the special issue preparation: Piramanayagam Shanmughavel (Bharatiar University, India), Haiqing Zhao (Columbia University Medical Center, USA), Konstantin Kozlov and Vitaly Gursky (St.Petersburg Polytech University, Russia), Olga Zolotareva (Bielefeld University, Germany), Alexey Kolodkin (University of Luxemburg, Luxemburg), Alexander Ratushny (Bristol Myers Squibb, USA), Olga Arkova (Institute of Gene Biology RAS, Moscow), Eric Mjolsness (Irvine University, USA), Shuan Li (University of Rhode Island, USA), Mengting Liu (University of Southern California, USA), Guohao Wang (NIH, USA), Nina Oparina (Karolinska Institut, Sweden), Anatoly Ivashchenko (al-Farabi Kazakh National University, Kazakhstan), Ekaterina Marakasova (US FDA, USA), Olga Tarasova (Sechenov University, Moscow, Russia), Oleg Gusev (RIKEN, Japan), Konstantin Gunbin (Novosibirsk State University, Russia), Hua Zhong (Fred Hutchinson Cancer Research Center, USA), Michael Linderman (Icahn School of Medicine at Mount Sinai, USA), Sergei Fedotov (MEPhI, Moscow, Russia), Gordon Crippen (University of Michigan, USA), Igor Berezovsky (Bioinformatics Institute, Singapore), Andreas Laner (Medizinisch Genetisches Zentrum, Munich, Germany), Mikhail Sadovsky (Siberian Federal University, Krasnoyarsk, Russia), Patrick Harrison (University College Cork, Ireland), Irina Medvedeva (Bristol Myers Squibb, USA) who helped at different stages of the materials processing and reviewing.

About this supplement

This article has been published as part of BMC Bioinformatics Volume 21 Supplement 11, 2020: Selected Topics in “Systems Biology and Bioinformatics” - 2019: bioinformatics. The full contents of the supplement are available online at -


Publication of this article was not covered by sponsorship.

Author information

Authors and Affiliations



TT and YO are guest editors of the special post-conference issues and Program Committee members of SBB-2019 School. EG is the invited editor and the organizing committee member of the conference. All the authors read, revised, and approved the final manuscript.

Corresponding author

Correspondence to Yuriy L. Orlov.

Ethics declarations

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Orlov, Y.L., Galieva, E.R. & Tatarinova, T.V. Bioinformatics research at SBB-2019. BMC Bioinformatics 21 (Suppl 11), 366 (2020).

Download citation

  • Published:

  • DOI: