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Fig. 1 | BMC Bioinformatics

Fig. 1

From: SPEAQeasy: a scalable pipeline for expression analysis and quantification for R/bioconductor-powered RNA-seq analyses

Fig. 1

An example samples.manifest. The samples.manifest file for paired-end samples is composed of five tab separated columns: (1) path to the first FASTQ file in the pair, (2) optional md5 signature for the first FASTQ file in the pair, (3) path to the second FASTQ file in the pair, (4) optional md5 signature for the second FASTQ file in the pair, (5) sample ID. The first two entries use the same sample ID, which is useful when a biological sample was sequenced in multiple lanes and thus generated multiple FASTQ files. The first two pairs of FASTQ files will be merged

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