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Fig. 4 | BMC Bioinformatics

Fig. 4

From: A novel automated image analysis pipeline for quantifying morphological changes to the endoplasmic reticulum in cultured human cells

Fig. 4

Definition of perinuclear dense RER structures. A–D Sequential key steps in the analysis shown on the same U-2 OS cell as in Fig. 2. Scale bars = 20 μm. A U-2 OS cell expressing the ER maker Sec61β-mEmerald (white). B Pseudo-coloured image based on Sec61β-mEmerald signal intensity, warm colours (red, orange, yellow) represent higher signal intensity, cold colours (pink, purple, blue) represent lower signal intensity. C Detection of dense ER (yellow area) after applying an intensity threshold to the Sec61β-mEmerald signal (white) within the cytoplasm (ROI). D Yellow region shows the surface of the cytoplasm, drawn in white, occupied by dense ER. E Steps A and D shown in a group of cells. Scale bar = 50 μm

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BMC Bioinformatics

ISSN: 1471-2105

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