Fig. 5

Drug-induced ER reorganisation in U-2 OS cells. A Representative images of ER (green) distribution in U-2 OS cells expressing Sec61β-mEmerald after treatment with vehicle (DMSO), Thapsigargin, Tunicamycin, Palmitic acid, Cytochalasin B and Bafilomycin A1. Polygon regions are seen to be enlarged (red arrowheads in enlarged images) or constricted (yellow arrowheads in enlarged images). Nucleus (blue). Scale bars = 20 μm top row images and 10 = μm lower row of enlarged images. B Quantification of ER polygon region average size. C Quantification of% of dense ER in cell cytoplasm. Data are expressed as mean ± SEM (n = 5 independent experiments comprising ≥ 50 cells each) and values significantly different from vehicle control were determined by one-way ANOVA and Tukey’s multiple comparisons test (**, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, P > 0.05)