Fig. 6

Analysis of ER distribution in U-2 OS cells using various ER markers. A Images of a single cell showing sequential analysis of SER polygon regions (columns 2 and 3) and dense RER (columns 4, 5 and 6) in U-2 OS cells expressing various constructs that label the ER (GOLT1B-YFP, ERIN2-YFP, SYVN1-YFP and MAGT1-YFP) (column 1). B Quantification of ER polygon region average size. C Quantification of % of dense ER in cell cytoplasm in U-2 OS cells expressing each ER-localising protein. D Representative images of U-2 OS cells showing sequential analysis of dense RER (columns 2, 3 and 4) labelled with anti-Reep5 antibody and ER Tracker™ (column 1) and quantification of % of dense ER in cell cytoplasm (E). Data are expressed as mean ± SEM (n = 3–4 independent experiments comprising ≥ 50 cells each). All scale bars = 20 μm