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- Open Access
Prediction of peptide drift time in ion mobility-mass spectrometry
BMC Bioinformatics volume 10, Article number: A1 (2009)
Understanding the proteome, the structure and function of each protein, and the interactions among proteins will give clues to search useful targets and biomarkers for pharmaceutical design. Peptide drift time prediction in IMMS will improve the confidence of peptide identification by limiting the peptide search space during MS/MS database searching and therefore reducing false discovery rate (FDR) of protein identification. A peptide drift time prediction method was proposed here using an artificial neural networks (ANN) regression model. We test our proposed model on three peptide datasets with different charge state assignment (see Table 1). The results can be found in Figure 1, where a higher prediction performance was achieved, over 0.9 for CI and C2, as well as 0.75 for C3.
In this study, an ANN regression model was developed to predict peptide drift time in IMMS. Three peptide datasets with different peptide charge states were used to train the predictor to capture the differences of drift time among the varied peptides. The high performance of predictor indicated the capacity of our proposed method. In addition, a simple net architecture, which consisted of an input layer with four neurons, a hidden layer with four nodes and an output layer with one neuron, make our model more effective for application of protein identification.
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This project was funded by 1R41RR024306.
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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Wang, B., Valentine, S., Raghuraman, S. et al. Prediction of peptide drift time in ion mobility-mass spectrometry. BMC Bioinformatics 10 (Suppl 7), A1 (2009). https://doi.org/10.1186/1471-2105-10-S7-A1
- Artificial Neural Network
- Hide Layer
- Charge State
- Protein Identification