- Meeting abstract
- Open Access
Flipping NextGen: using biological systems to characterize NextGen sequencing technologies
© Glasscock et al; licensee BioMed Central Ltd. 2009
- Published: 25 June 2009
- Genome Project
- Sequence Length
- Sequence Space
- Combinatorial Library
- Sequencing Project
At a current 12 gigabases per sequencing run (and growing), there have been significant advancements in DNA sequencing technologies resulting in next generation (NextGen) sequencing platforms that produce 5 orders of magnitude more data than platforms used for the human genome project.
Transcriptomes on the other hand were much less variable and required fewer bases (x) to uniquely map a much larger percentage (y) of their sequence space. For example, more than 98% of the complex human transcriptome could be uniquely characterized with as few as 20 bp.
Finally, de-novo sequencing (i.e. without a reference) would require a minimum of 1/2 of the sequence length to be unique in order to allow sufficient contig extension in the assembly process. For example, 40–50 bp reads are necessary for de-novo characterization of these systems uniquely defined by 20–25 bp reads. As of 2009, short read NextGen sequencing technologies have moved to 50 bp and beyond, ushering in what is expected to be the start of a revolution in genomics.
These results establish a lower bound on sequence length (x) required to sufficiently conduct re-sequencing, transcriptome, and de-novo sequencing projects. The asymptotic nature of the results also provides a guide for what percentage of the total space (y) we might expect to define in genomes/transcriptomes of similar size and complexity.
This article is published under license to BioMed Central Ltd.