Computational investigation of DNA packing in confinement
© Ziegler et al; licensee BioMed Central Ltd. 2012
Published: 31 July 2012
High-density packing of DNA in nature, for example in mutant P4 bacteriophage viruses and the resulting entanglement (= knots) of the packaged DNA has been a focus of recent research across several disciplines . Published data allows the conclusion that the ends of the DNA packaged in P4 meet in the capsid creating a circular molecule and that a high percentage of knots is formed due to the effect of the capsid's confinement. This report describes a computational investigation of packing DNA in confinement.
Materials and methods
In this study DNA is modeled as a freely-jointed closed chain of unit-length segments, called a polygon and confinement is modeled by a sphere. Packaging is modeled by randomly generating polygons which fit inside the sphere. In a 'local' model, obeying confinement only matters when the segment might actually breach it . A 'global' model biases the generation of every segment to avoid a future breach of the boundary . For each model its (cumulative) probability density function - in terms of the distance d of the next vertex from the origin - was derived which is the basis for the algorithm generating such polygons with uniform probability.
The starting and the end vertex of the polygon is the origin and the second vertex is on the unit sphere. For each successive vertex, use the cumulative probability density function to select uniformly the distance d of the next vertex from the origin. The next vertex is then chosen uniformly on the intersection circle of the unit sphere around the current vertex and the sphere with radius d around the origin. The second to last vertex is chosen uniformly from the intersection circle of the unit spheres around the current vertex and the origin.
This work is supported in part by NSF Grants #DMS-0920880 and #DMS-1016460 (Y. Diao), and by NSF grant #DMS-1016420 (C. Ernst, A. Montemayor and U. Ziegler).
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