MOIRAI: a compact workflow system for CAGE analysis
© Hasegawa et al.; licensee BioMed Central Ltd. 2014
Received: 10 December 2013
Accepted: 6 May 2014
Published: 16 May 2014
Cap analysis of gene expression (CAGE) is a sequencing based technology to capture the 5’ ends of RNAs in a biological sample. After mapping, a CAGE peak on the genome indicates the position of an active transcriptional start site (TSS) and the number of reads correspond to its expression level. CAGE is prominently used in both the FANTOM and ENCODE project but presently there is no software package to perform the essential data processing steps.
Here we describe MOIRAI, a compact yet flexible workflow system designed to carry out the main steps in data processing and analysis of CAGE data. MOIRAI has a graphical interface allowing wet-lab researchers to create, modify and run analysis workflows. Embedded within the workflows are graphical quality control indicators allowing users assess data quality and to quickly spot potential problems. We will describe three main workflows allowing users to map, annotate and perform an expression analysis over multiple samples.
Due to the many built in quality control features MOIRAI is especially suitable to support the development of new sequencing based protocols.
The MOIRAI source code is freely available at http://sourceforge.net/projects/moirai/.
The next generation sequencing has taken a central role in genomics and transcriptomics. In recent years both the amount of data generated per sample and the number of samples used in a particular study has increased dramatically. Robust data processing workflows and a data management system are essential to deal with the flood of data.
Among assays to study the transcriptome cap analysis of gene expression (CAGE) technology specializes on detecting the 5’ end of transcripts [1, 2]. RNAs can be quantified by counting the number of reads at a particular transcriptional start site (TSS). CAGE profiling has revealed an unexpected transcriptional complexity in both human and mouse . More recently CAGE has been used in the Encyclopedia of DNA Elements (ENCODE) project to detect TSS in several human cell lines , improve gene annotations  and to provide anchor points for epigenetic and other types of data .
Essential steps in the data processing of CAGE data include initial demultiplexing and read trimming steps, filtering, mapping reads to the genome and clustering of the mapped reads into peaks. The latter can be used to discover novel regulatory motifs and detect differential promoter usage among several samples.
To automate and standardize the analysis of CAGE data we created a web based workflow system called MOIRAI. Our goal was to create a simple system usable by both wet and dry scientists while at the same time providing an appropriate level of flexibility to meet project specific challenges. We include workflows to support the analysis of tagging-CAGE  and nano-CAGE  datasets.
Much like other workflow systems such as Galaxy , a MOIRAI workflow strings together several tools to process data in a stepwise fashion. Workflows can be created and modified using a built in graphical editor. The latter can be run as a stand alone application to prototype workflows before incorporating them into a web-based instance of MOIRAI.
Once fixed, users can run workflows by specifying input files and parameters via a simple web interface. A daemon in the background identifies pending jobs and executes them either locally or on a compute cluster. For each individual step in a workflow MOIRAI keeps track of the processing time, file sizes and potential error messages. All the results are written to a single directory containing a html page displaying the workflow used, detailed information about all programs/parameters used and links to the output files. In larger collaborations, it is beneficial to share the entire MOIRAI output as it contains both the data and the exact description of all processing steps. Several additional tools are included to simplify the installation and updating of analysis software, reference genomes and setting of in- and output directories per user.
MOIRAI can be installed on UNIX based operating systems (tested on MacOSX, Ubuntu and Fedora) running Apache and PHP services. To make installation easy, we prepared an automatic script to set up MOIRAI on user’s personal computer. Using it, users will be able to run a default "Hello World" test workflow included in the download package after minutes of installation. Other CAGE workflows require the installation of analysis software, reference genomes and configuration of input and output directories. We provide the tools and scripts to automate these steps.
Each box in a MOIRAI workflow represents one process and an arrow between units describes a flow of the data. When a unit has multiple inputs, it will not be executed until all previous computations are completed. Furthermore, in the case of paired-end sequencing data, MOIRAI will verify that input files belong to the same sample by matching the file names. All units are colored to reflect three basic types of entities. Input files and reference databases are colored green, intermediate steps producing temporary files are gray and finally all outputs are shown in blue. For quality control purposes, we provide graphs embedded within the workflow to summarize the results at key steps. Similarly, thumbnails of windows looking similar to unix terminals allow users to check summary statistics and look at the most frequent sequences.
To add flexibility, MOIRAI contains a standardized web-based mechanism to incorporate new command line tools and methods into the system. In brief, users can make an entry into an online table. Each entry requires the user to set the name of the program, the in- and output file formats, options and default parameters. It is possible to include the same program several times but with different parameter options to customize the resulting unit for particular types of data. Once incorporated these units can be used to create new workflows or modify existing ones.
Importing SAM to BAM functionality
samtools view -bSo [output] [input]
Importing BWA align functionality
bwa aln -n [error-rate] [reference] [input] > [output]
Tools available in MOIRAI
Demultiplexing for CAGE
Remove artificial sequences
Remove rRNA sequence
Statistics of reads
Draw PNG graphs from text table
CAGE tags from different libraries are tagged with barcode sequences and pooled into one sequencing lane to improve sequencing capacity and to enable accurate comparison between samples . We wrote tools to demultiplexing these pooled tags into separate outputs according to barcode sequences for both single-end and paired-end CAGE. The MOIRAI workflow system will automatically recognize that demultiplexing units produce multiple files and will apply all downstream steps to all the files.
After demultiplexing we use two tools to remove unwanted sequences. TagDust  removes reads similar to primer dimers and other artifacts while rRNAdust removes reads matching ribosomal RNA sequences.
The tome database system is a simple implementation of a compressed sparse row data structure to store mapped CAGE reads from multiple experiments. The database can be queried to extract the number of CAGE reads within genomic boundaries given in bed6 format. The latter can be used to calculate the fraction of reads in each library mapping to promoters and to cluster biological samples.
SAMstat  displays mismatch, insertion/deletion error profiles, mapping rates and many other useful statistics of FASTQ/SAM/BAM sequence reads in HTML format. The report helps users to spot biases and problems in sequencing runs and protocols.
In addition we included several generic plotting tools to display the base distribution in intermediate sequence files and to list the most frequent sequences. These tools are extremely useful to spot and correct problems in complicated pipelines.
Summary of results
Once a workflow is completed, MOIRAI summarizes the results and additional information on the run itself in a web page. The latter includes paragraphs describing each step including the version and parameters of the software used. By default all standard error and output messages are recorded per process and are stored in a separate html page. The run time and resulting file sizes of each step can be viewed by moving the mouse over the corresponding box in the workflow. Finally, the main output files are accessible by clicking on blue output boxes.
In addition to the results, we include a copy of the workflow template used for the computation itself. The workflow can be imported into another user’s account or MOIRAI instance to precisely reproduce all the results.
We included a workflow editor that can be accessed via the web-interface or as a stand alone application. The advantages of using the stand alone application include dragging and dropping data files directly from the desktop into the editor, offline editing of workflows as well as the ability to selectively execute only the recently added steps. The latter is desirable when debugging large workflows with time consuming initial processing steps. The mechanism for incorporating new tools and setting parameters is simple.
ENCODE CAGE K562 Libraries
CAGE mapping workflow
A multiplex sequence and a "CAGCAG" linker sequence at the beginning can be readily identified in the top left plot showing the base distribution of the raw reads. After demultiplexing and read trimming the base distribution is much more even (middle left plot). By default, we remove reads containing ambiguous bases (N). We can see from the second plot on the left that these low quality bases (shown in black) accumulate at the 3’ end of the reads which is typical for the Illumina sequencer .
Finally, a bar graph at the end of a workflow summarizes how many reads passed each stage. In the example shown we conclude that the CAGE reads are of high quality since only 2.4% of sequences were removed by filtering steps, and more than 90% of the filtered tags were successfully mapped.
Based on our experience, we consider a CAGE library to be of acceptable quality if fewer than 10% of the reads correspond to ribosomal RNA and if the mapping rate higher than 70%.
In the case of the ENCODE CAGE libraries, it is evident that libraries obtained from nuclear sub-compartments have fewer reads mapped to promoters (100bp upstream and 5’UTR category) compared to the other libraries. As expected, the polyA+ libraries have high percentage of promoter regions compared to nuclear compartments as most measured RNA molecules are messenger RNAs.
Expression analysis workflow
Finally, the expression analysis workflow groups mapped reads into peaks using a parametric clustering algorithm implemented in the program Paraclu . In brief, this method reports genomic intervals containing many more CAGE reads than surrounding regions. These regions can be contained within each other giving rise to hierarchies of clusters. From this we select all clusters of length ≤ 200bp and with a stability greater than two. Normalized expression values for these peaks in multiple samples is extracted using the tome program.
The dendrogram graph is drawn by pvclust where probability values (p-values) are computed for each cluster with bootstrap resampling techniques . If approximately unbiased (AU) p-value and bootstrap probability (BP) value are not necessary, bootstrap can be switched to computationally quicker hierarchical clustering (hclust). Biological replicas cluster together and libraries obtained from the whole cell and cytoplasmic fraction cluster closer together than nuclear libraries. One cytosolic polyA+ replica is clustered far away from other two replicate libraries.
The heatmap is another way of visualizing the overall similarity among samples. The graph shows two clusters of nuclear sub-compartments and cytoplasmic fractions with nuclear polyA+ replicates in the middle. It is interesting to note that nucleus polyA+ libraries appear to be similar to both nuclear and cytoplasmic libraries. Light green patterns appearing between all polyA+ samples indicates large number of mRNAs with polyA+ in nucleus are transported out to cytosol.
MOIRAI allows users of all skill levels to carry out the most essential steps in the processing and analyzing CAGE data. A key feature is the integration of analysis software and quality control programs in the same workflow. The latter is very useful in troubleshooting but also in getting a sense of computational bottlenecks and general flow of the data.
Finally, the output of MOIRAI combines result files with a html page showing the workflow used, all included programs, their versions and intermediate results. We believe this combination makes it very clear what was done to the data and facilitates reproducible research . A copy of the workflow used is included in the results to make it easy to run exactly the same workflow on new data.
MOIRAI is the right tool for processing and analyzing CAGE reads. It is simple to use, provides flexibility to adjust analysis workflows/pipelines when needed while being capable of dealing with large amounts of NGS data.
Furthermore, we plan to expand workflows to downstream analysis including large scale data integration.
While we have focussed here on CAGE data, the inherent flexibility of MOIRAI makes it possible to generate workflows to process RNAseq, Chip-Seq and other types of data.
Availability and requirements
Project name: MOIRAI
Project home page: http://sourceforge.net/projects/moirai/
Operating systems: Unix/Linux or Mac
Programming language: java, perl, php, c, bash
Other requirements: R, sqlite3, and Java SE (Standard Edition).
License: GNU General Public License version 3.0 (GPLv3)
Others: The MOIRAI package is also available from the fantom web resource (http://fantom.gsc.riken.jp/software/).
This work was supported by a Research Grant from the Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT) to the RIKEN Center for Life Science Technologies, a research grant for RIKEN Omics Science Center from MEXT to YH and a grant of the Innovative Cell Biology by Innovative Technology (Cell Innovation Program) from the MEXT, Japan to YH.
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