- Open Access
Metavir 2: new tools for viral metagenome comparison and assembled virome analysis
© Roux et al.; licensee BioMed Central Ltd. 2014
- Received: 10 July 2013
- Accepted: 27 February 2014
- Published: 19 March 2014
Metagenomics, based on culture-independent sequencing, is a well-fitted approach to provide insights into the composition, structure and dynamics of environmental viral communities. Following recent advances in sequencing technologies, new challenges arise for existing bioinformatic tools dedicated to viral metagenome (i.e. virome) analysis as (i) the number of viromes is rapidly growing and (ii) large genomic fragments can now be obtained by assembling the huge amount of sequence data generated for each metagenome.
To face these challenges, a new version of Metavir was developed. First, all Metavir tools have been adapted to support comparative analysis of viromes in order to improve the analysis of multiple datasets. In addition to the sequence comparison previously provided, viromes can now be compared through their k-mer frequencies, their taxonomic compositions, recruitment plots and phylogenetic trees containing sequences from different datasets. Second, a new section has been specifically designed to handle assembled viromes made of thousands of large genomic fragments (i.e. contigs). This section includes an annotation pipeline for uploaded viral contigs (gene prediction, similarity search against reference viral genomes and protein domains) and an extensive comparison between contigs and reference genomes. Contigs and their annotations can be explored on the website through specifically developed dynamic genomic maps and interactive networks.
The new features of Metavir 2 allow users to explore and analyze viromes composed of raw reads or assembled fragments through a set of adapted tools and a user-friendly interface.
- Web server
Viruses are the most abundant biological entities in the biosphere  and are now considered as major players in natural ecosystems and their associated cycles and balances [2, 3]. Viral communities are known to be mostly composed of new strains [4–6] and are difficult to characterize as (i) most micro-organisms are still impossible to cultivate in the lab for now, hence preventing the culture, isolation and study of their associated viruses and (ii) the absence of a single gene common to all viral genomes prevents the monitoring of uncultured viral diversity using approaches analogous to ribosomal DNA profiling.
Metagenomic approaches, consisting in a random sequencing of the genetic pool isolated from natural samples, circumvent these limitations. Experimental protocols to extract and isolate the encapsidated fraction are now well established [7–9], and viral metagenomes (i.e. viromes) have been generated from a broad range of ecosystems. Beyond the description and characterization of the viral genomic diversity, viromes are useful towards more general questions such as biogeography and dispersion of viral particles [10, 11], evolution and origin of viruses  or epidemiology .
Advances in next-generation sequencing and in sequence assembly techniques recently led viral metagenomics a step further, by providing access to large genomic fragments rather than only short reads [14–16]. Indeed, contigs representing complete or near-complete viral genomes were assembled from 454 [17–20] and Illumina HiSeq [21–23] generated viromes. These large assembled sequences (several Kb or tens of Kb, depending on the diversity of the viral community studied) provide access to the genome content and architecture of uncultured viruses and offer the possibility to gain unique insights into the main viral families in the environment.
Two web-servers are currently available for a comprehensive virome analysis: Metavir , and Virome . A pipeline (the Viral Metagenome Affiliation Pipeline ) was also described but to our knowledge is not available neither as a standalone software or through a web page. Yet, none of these bioinformatic tools were designed for the analysis of assembled datasets and the absence of adapted tools for such assembled viromes was pinpointed as a major bottleneck for viral metagenomic studies [25, 27]. Moreover, the growing number of generated viromes calls for the development of comparison strategies to go beyond individual analysis of each dataset. Here, we introduce a new version of Metavir that tackles these two limitations. Metavir 2 includes (i) new ways to compare datasets and (ii) a whole new section which forms the first tool designed for a comprehensive analysis of assembled virome sequences.
Input and metadata
Registered users can upload their own sequence datasets, either short reads or assembled contigs, in a private space. Input data are checked for being only composed of DNA sequences in fasta format (compressed files in zip, gzip or tar.gz format are accepted). Due to the size of Illumina’s raw datasets (~50 Gb) and computing time required for assembling each dataset, the assembly step cannot be computed through Metavir. Furthermore, a wide range of softwares are available for this step and the choice depends on the type of the sequencing and the nature of the sample: Newbler (454 Life Sciences) is the main software used so far for 454 data [20, 28, 29], and Illumina data can be assembled with Idba_ud , SOAP , MetaVelvet  or OptiDBA .
A set of public viromes is also already available for users to compare with their dataset(s). These viromes are sorted into projects, and linked to the manuscript describing their analysis when available. Various metadata can be added, such as the type of sample from which the virome was sequenced, the location, depth, and temperature of sampling point, and the sequencing technology used to generate the dataset.
Section 1: tools to analyze raw datasets (unassembled reads)
k-mer frequency bias
A virome comparison based on k-mer frequency bias (di-, tri- and tetranucleotides are available) has been implemented as described by Willner and collaborators . Unlike the other available comparison method, based on sequence similarity (generated using reciprocal tBLASTx) and requiring datasets containing at least 50,000 sequences of 100bp, k-mer nucleotide frequencies can be computed for all datasets without size restriction. Briefly, k-mer frequency distribution bias are computed by a custom Perl script and then compared for each pair of viromes. Pairwise euclidian distances between viromes are stored in a matrix, which can be used as input either in a hierarchical clustering or a non-metric multidimensional scaling. Both analysis are computed with R  using pvclust  and vegan  libraries respectively. The non-metric multidimensional scaling (NMDS) is now also available for virome comparison based on sequence similarities, available in Metavir 1.
Individual viral genome recruitment plots
Using the best BLAST hit results against RefseqVirus, each virome sequence with a hit is affiliated to a unique viral genome, i.e. each read is recruited by a reference virus. For any selected viral genome, two types of recruitment plots are then available: (i) a scatter plot displaying each recruited read as a dot depending on the position on the genome (on the x-axis) and the identity percentage of the BLAST hit (on the y-axis), and (ii) an histogram presenting the number of recruited reads for each 500-nt long genome part. These plots are generated using the ggplot2 R library . Additional viromes that contain sequences recruited by the selected genomes are also listed and can be added to the current plot. When several datasets are selected, a color is attributed to each virome, used to color dots (in scatter plots) or stacked histograms (in histograms).
Section 2: assembled viromes annotation and display
Open reading frames (ORFs) are first predicted for each contig through MetaGeneAnnotator . A custom Perl script was designed to detect circular contigs by looking for identical k-mer at the two ends of the sequences. Each circular contig is then trimmed to remove all redundant parts. In order to be able to predict genes spanning the origin of circular contigs, a temporary version of circular contigs is used in the ORF prediction software, in which the first 1,000 nucleotides are duplicated and added at the contig’s end. It has to be noted that this detection of circular contigs will not be effective for contigs computed with assembler like Newbler which already detect and remove such similarity between contig ends.
All predicted translated ORFs are then compared to several databases, namely the RefseqVirus protein database from the NCBI using BLASTp , with a threshold of 10−3 on e-value, and the PFAM database of protein domains (version 26.0; ) using HMMScan , with a threshold of 30 on score. A direct comparison of ORFs within a virome is also computed through a BLASTp with the same threshold of 10−3 on e-value.
The taxonomic composition and sequence diversity are not calculated the same way for datasets made of long genomic sequences compared to those made of short reads. Using the BLASTp results against reference viruses, three types of taxonomic compositions are computed for each dataset. These compositions are based on (i) best BLAST hit affiliation of each predicted gene, (ii) best BLAST hit affiliation of each contig, and (iii) lowest common ancestor affiliation of each contig. This LCA affiliation is designed to take into account the multiple hits on a single contig: up to five affiliated genes (if available) are considered for each contig, and the affiliation is made at the highest common taxonomy level of the best BLAST hit from these selected genes.
Finally, different clusterings of the predicted ORFs are computed. A global protein sequence clustering with three different thresholds (75, 90 and 98% of similarity) is performed using Uclust . Another clustering is based on protein domain alignments: ORFs are first ordered by size, and used iteratively as a seed for a jackhmmer search . All ORFs recruited by the seed are gathered in a cluster with this seed, and removed from further iterations. Once computed, the domain-based ORFs clusters are affiliated to one or more PFAM domain based on the affiliation of their members. These clusterings are displayed through the rarefaction curve tool, and cluster affiliations can be downloaded in a csv file.
When an assembled virome is selected, a new “contig maps” page now provides general informations about ORF prediction and contig affiliations, as well as an inset that allows to filter the contig list and access contigs of interest for further analysis (contig maps and networks). This interactive filter, developed using Jquery, let users select contigs based on taxonomic or functional affiliations of predicted genes, and contig size, name or taxonomic affiliation.
An interactive genomic map can be displayed for each contig, this map being drawn using RaphaelSVG and the Raphael-zpd plugin. Each gene affiliation to Refseq viral genomes and PFAM protein domains is indicated when available. Genes can be further investigated as nucleotide and protein sequences are displayed by clicking on the gene either on the map or on the gene table below. Contig annotations can also be downloaded as csv tables, summarized by contig or detailed for each ORFs.
Similarities between contigs and viral genomes and between different contigs can be visualized as an interactive network. In order to take into account all relevant similarities and not only the best BLAST hit for each ORF, all BLAST hits with an e-value lower than 10−3 and having a bit-score within a 10% margin from the best BLAST hit bit-score for this ORF are used to build the contig network. In the resulting networks created with Cytoscape-web , contigs and reference genomes are represented as nodes, and sequence similarities as edges. Different options are available to customize the network, such as the coloring of edges based on BLAST bit-score, the display of only one edge between two similar contigs or of one edge for each ORFs similarity, or the coloring of genome nodes based on the taxonomy. Another set of filters is also proposed to reduce the number of nodes or edges displayed on screen.
Associated with this network, a contig map comparison tool can be used to display collinearity between contigs and genomes or other contigs selected on the network. This comparisons are displayed through RaphaelSVG and Raphael-zpd. Name and affiliation of each gene is displayed when clicked, and a Jquery pop-up is used to change the sequence order within the plot.
Automatic database update
As the RefseqVirus database is quickly growing (40 new genomes are added on average every month), each new release is automatically downloaded and used as the new reference database. Taxonomic composition, gene affiliation (for contig dataset), and recruitment plots of public projects are automatically updated with each release, whereas the update of private projects must be requested by the user.
Results and graphics download
All sequence datasets used in a Metavir analysis are available for download in fasta format (affiliated and uncharacterized sequences, sequences included in phylogenetic trees and sequences included in recruitment plots). All tables (taxonomic heatmap, contig and ORF affiliations, results for recruitment analysis) can be downloaded as csv files that can be imported in spreadsheet softwares.
Contig annotations are available in GenBank file format, which can be used in many downstream tools like Artemis  or Easyfig . These GenBank files contain the lowest common ancestor affiliation of the contig, as well as the best BLAST hit affiliation of each ORF, the functional annotation of each ORF in PFAM domain, and the sequences of each predicted CDS.
All interactive charts and pictures (contig maps, contig comparisons, phylogenetic trees) can be downloaded in svg format, a publication-ready vectorial format easy to modify using graphics softwares. Static charts generated with R are available to download in pdf and png file format.
Finally, the contig networks can be downloaded in a set of different formats, including graphml and xgmml, ready to be imported in the desktop version of Cytoscape for further analyses and annotations.
Case study: using metavir to analyze the human gut virome
Two different datasets from the human gut viral community were chosen to illustrate the results that can be obtained with Metavir 2. First, a set of 16 viromes was used to illustrate the section dedicated to unassembled datasets (; project “Human Gut Diet” on Metavir). These metagenomes, sequenced with 454 GS Titanium (884,628 reads of 350 bp/310 Mb), were initially designed to study the dynamics of human gut viral community during a perturbation by a dietary intervention. Two individuals were fed a high fat/low fiber diet (H1 and H2), three were fed a low fat/high fiber (L1, L2 and L3) and one was on an ad-lib diet (X). Samples were collected at up to four time points (days 1, 2, 7 and 8). The second dataset is an assembled virome, resulting from the assembly of Illumina Hi-Seq 2000 reads (5.6 Gb of 100 bp reads) from healthy individuals (; virome “Human gut – All subjects” from project “Human Gut Assembly” on Metavir). This assembled dataset was used here to illustrate the possibilities offered by the new section dedicated to the analysis of contigs.
Metavir, a web server dedicated to the analysis of viromes uploaded by registered users, can now be used to analyze the two existing types of datasets: (i) viromes composed of raw reads, mostly generated using pyrosequencing technology and (ii) viromes assembled into contigs, a strategy possible with datasets sequenced with either pyrosequencing or Illumina technology. The novelties of version 2 of Metavir will be illustrated here using both types of datasets (unassembled 454 reads  and Illumina assembled contigs ), all from human gut samples.
Additions to the unassembled datasets section
Most published viral metagenomes are still analyzed at the read level. Indeed, pyrosequencing technology is often chosen to generate viromes, as this technology produces long reads and several samples can be easily multiplexed in a single run. Thus, the number of reads in each multiplexed dataset is generally insufficient to produce an assembly. Furthermore, the multiple datasets generated make it possible to study spatial or temporal dynamics in environmental communities [10, 22, 50–52] or different individuals subjected to different conditions for eukaryote-associated viromes (e.g. different diets in ). In this context, the comparison of multiple datasets was our major focus while extending the section dedicated to unassembled datasets. In addition to the rarefaction curves and reciprocal tBLASTx comparison available in the initial version of Metavir, taxonomic compositions and phylogenetic analyses can now be used to compare viromes. Furthermore, most of these tools were improved with special attention to the display of results. A brand new tool was also added: the recruitment plot analysis, which makes it possible to accurately study the similarities between virome reads and a viral genome of interest.
k-mer frequency bias
Individual viral genome recruitment plots
Analyzing assembled datasets using the new contig section
Even though unassembled viromes proved to be useful for a better characterization of environmental viral communities, long genomic fragments generated through the assembly of metagenomic datasets are usually more informative. Indeed, complete ORFs predicted out of such contig sequences (i) are more often similar to known viruses than short reads , (ii) provide more robust phylogenies than using reads representing only a portion of a gene, and (iii) are more appropriate than short random reads in determining the gene content and genetic diversity of a viral community . Moreover, analysis of the genomic content and architecture can provide decisive insights into virus classification and evolution of viral groups .
A new section dedicated to the annotation and navigation within sets of contigs has therefore been implemented in Metavir. When assembled viromes, i.e. sets of contigs, are uploaded by users, ORFs are predicted  and then annotated using sequence similarity results against viral genomes and protein domains. In addition to the general taxonomic composition, contig maps and annotations can be displayed for every contig. As datasets can consist of tens of thousands contigs, users can choose to visualize contigs (i) longer than a defined threshold, (ii) predicted as circular or linear, (iii) affiliated to a particular viral family, and/or (iv) possessing a particular gene. Finally, tools available for read analysis were specifically adapted to assembled datasets: taxonomic compositions are computed using either gene or contig affiliation, phylogenies are generated using predicted ORFs and genetic diversity is computed using either predicted ORFs or domain conservation.
This new release of Metavir provides a wide range of tools to analyze either raw or assembled viral metagenomes in a comprehensive way. As virome projects now regularly encompass multiple samples and as more and more viromes are being published, a special effort was made towards virome comparison. Two new large scale methods were implemented and all existing Metavir tools were modified so that they can be used to compare datasets. Furthermore, a new section has been specifically developed to handle sets of large genomic contigs. As these datasets can be large and as all individual sequences can be of interest, we paid special attention to the interface, with filtering panels and network visualization. Selected contigs can then be analyzed in detail by comparing their automatic annotations in terms of gene content and genomic maps. Finally, with its extended or new tools and sections, Metavir 2 provides a comprehensive framework with a user-friendly interface to explore any kind of viromes, and should help virologists to make the most of their metagenomics data.
Project Name: Metavir
Project home page: http://metavir-meb.univ-bpclermont.fr
Operating system(s): Platform independent
Licence: GNU GPL3
Any restrictions to use by non-academics: No.
SR was supported by a PhD grant from the French defense procurement agency (DGA, Direction Générale de l’Armement). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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