- Open Access
Satellog: A database for the identification and prioritization of satellite repeats in disease association studies
© Missirlis et al; licensee BioMed Central Ltd. 2005
Received: 12 January 2005
Accepted: 10 June 2005
Published: 10 June 2005
To date, 35 human diseases, some of which also exhibit anticipation, have been associated with unstable repeats. Anticipation has been reported in a number of diseases in which repeat expansion may have a role in etiology. Despite the growing importance of unstable repeats in disease, currently no resource exists for the prioritization of repeats. Here we present Satellog, a database that catalogs all pure 1–16 repeat unit satellite repeats in the human genome along with supplementary data. Satellog analyzes each pure repeat in UniGene clusters for evidence of repeat polymorphism.
A total of 5,546 such repeats were identified, providing the first indication of many novel polymorphic sites in the genome. Overall, polymorphic repeats were over-represented within 3'-UTR sequence relative to 5'-UTR and coding sequence. Interestingly, we observed that repeat polymorphism within coding sequence is restricted to trinucleotide repeats whereas UTR sequence tolerated a wider range of repeat period polymorphisms. For each pure repeat we also calculate its repeat length percentile rank, its location either within or adjacent to EnsEMBL genes, and its expression profile in normal tissues according to the GeneNote database.
Satellog provides the ability to dynamically prioritize repeats based on any of their characteristics (i.e. repeat unit, class, period, length, repeat length percentile rank, genomic co-ordinates), polymorphism profile within UniGene, proximity to or presence within gene regions (i.e. cds, UTR, 15 kb upstream etc.), metadata of the genes they are detected within and gene expression profiles within normal human tissues. Unstable repeats associated with 31 diseases were analyzed in Satellog to evaluate their common repeat properties. The utility of Satellog was highlighted by prioritizing repeats for Huntington's disease and schizophrenia. Satellog is available online at http://satellog.bcgsc.ca.
Anticipation is a medical observation that refers to the progressive worsening of a disease's symptoms and/or an earlier age of onset over successive generations of affected family members . Although historically controversial, the concept gained widespread scientific acceptance with the identification in 1991 of unstable trinucleotide repeats associated with Fragile X syndrome [2, 3] and spinal and bulbar muscular atrophy (SBMA) . Today, 35 human diseases, some of which also exhibit anticipation, have been associated with unstable repeats . Diseases for which unstable microsatellites are the causative disease mechanism can be divided into those caused by coding or non-coding repeat expansions.
The majority of disease-associated coding repeats identified to date are CAG-type repeats encoding an expanded poly-glutamine tract in affected individuals. CAG-type expansion disorders include spinal and bulbar muscular atrophy (SBMA) , dentatorubral-pallidoluysian atrophy (DRPLA) , Huntington disease (HD)  and a range of spinocerebellar ataxias (SCAs) including SCA1 , SCA2 , SCA3 , SCA6 , and SCA7 . In these diseases, an expanded poly-glutamine tract results in a toxic gain of function causing either neuronal degeneration , or in mouse models of spinocerebellar ataxia (SCA), neuronal dysfunction due to Purkinje cell abnormalities . The precise pathogenic disease mechanism is unknown but requires expression of the expanded polyglutamine tract. Neuronal inclusion bodies are observable on autopsy .
Untranslated repeats are diverse and include non-trinucleotide repeats. For example, progressive myoclonic epilepsy type 1 (EPM1) pathology results from an expansion of the dodecamer CCCCGCCCCGCG  and an ATTCT repeat expansion is the pathogenic agent in SCA10 . In contrast to the coding repeat disorders, non-coding repeats can expand dramatically into the range of thousands of repeats . Most non-coding repeat expansions are not associated with neuronal inclusion bodies on autopsy , with the exception of Fragile X-associated tremor ataxia syndrome , and nuclear foci observed in neurons of myotonic dystrophy patients .
Anticipation has been reported in a number of orphan diseases in which repeat expansion may have a role in etiology. These diseases include autosomal dominant limb-girdle muscular dystrophy , Crohn's disease , leukemia , nodal osteoarthritis , Parkinson's disease , rheumatoid arthritis , truncal heart defects , mood disorders , schizophrenia [28, 29], and anxiety disorders [30, 31]. Although no repeat expansions have been associated with any of these disorders, no comprehensive surveys have been undertaken.
Historically if one suspected a polymorphic microsatellite repeat were associated with a disease, few bioinformatics resources were available to identify relevant repeats in the human genome. One approach now available is to browse the Tandem Repeats Finder (TRF)  track on the UCSC genome browser  within a genomic region of interest. TRF at UCSC was executed with liberal insertion and deletion (indel) and substitution penalties that allow the detection of larger, frequently impure repeats. Since pure repeat tracts are more likely to expand than impure repeat tracts following transmission [34–36] a large fraction of repeats presented at UCSC are probably not relevant for disease association studies. Furthermore, certain known disease-associated repeats, such as the GAA repeat in Friedreich's Ataxia (chr9:67,109,320-67,109,339) , are not detected at all at UCSC because they are too short to be detected by their TRF parameters. Other groups have created databases of all 2–16 repeat unit satellite repeats within human gene regions [38, 39] and of all 1–6 repeat unit microsatellites across prokaryotic and eukaryotic taxa . Collins detected microsatellites with a novel algorithm and deposited this data in a relational database called GRID Short Tandem Repeats (STR) database . This database included in silico polymorphism detection of coding trinucleotide repeats by using the BLAST algorithm to detect each repeat's length polymorphisms within GenBank, but only for a subset of coding repeats . These resources enrich the microsatellite repeat bioinformatics landscape but do not integrate these data with other published resources in a way relevant for repeat prioritization in disease-association studies. Also, these resources do not provide flexible interfaces for combining data in user-defined ways to allow dynamic generation of candidate repeat lists. For example, both the Microsatellites Repeat Database (MRD)  and the STR databases  provide static co-ordinates of candidate repeats for disease-association studies defined by the author's criteria, but lack the functionality to easily re-prioritize repeats based on user preferences.
To address these deficiencies we created Satellog, a database that catalogs all pure 1–16 repeat unit satellite repeats in the human genome along with supplementary data we believe to be of use for the prioritization of satellite repeats in disease association studies. For each pure repeat Satellog can also calculate the percentile rank of its length relative to other repeats of the same class in the genome, its polymorphism within UniGene clusters , its location relative to known genes , and its expression profile in normal tissues according to the GeneNote database . Repeats within Satellog can be prioritized based on any of their characteristics (i.e. repeat unit, class, period, length, length percentile rank, genomic co-ordinates), polymorphism profile within UniGene, proximity to or presence within gene regions (i.e. cds, UTR, 15 kb upstream etc), metadata of the genes they are detected within, and gene expression profiles within normal human tissues. Disease-associated repeats from 31 diseases were used as a test set to see what fraction could be detected independently within Satellog and what could be learned about polymorphic repeats in general. To showcase its utility, we used Satellog to prioritize repeats for disease-association studies in Huntington's disease and schizophrenia. Satellog is available as a web-queriable database along with all source code licensed under GNU General Public License at http://satellog.bcgsc.ca.
Unstable coding repeats organized by descending standard deviation Sample output from Satellog.
Characteristics of disease-associated repeats
Disease-associated repeats and their common properties were recently reviewed . We queried the database with these sequences to observe any characteristic features of these repeats relative to all other repeats. We asked how many of these repeats could be identified as potentially unstable using only the bioinformatics resources within Satellog. The co-ordinates for 31 of the 35 disease-associated repeats were manually collected from the review and identified in Satellog. Repeats that were not analyzed either had a repeat period greater than 16 (thus not detected by our TRF parameters) or were polymorphic but not associated with any disease. For these disease-associated repeats, there is no record of their precise genomic co-ordinates. To address this, we used Satellog to probe for the probable repeat that corresponded to each disease by selecting all repeats of the expected class within each disease gene. All repeats were detected, except for the repeat responsible for blepharophimosis . In 12 cases, more than one candidate was detected as the disease-associated repeat for a disease. These cases usually involve flanking repeats of the same class that are detected as two distinct repeats because of an interrupting unit, an established characteristic of some disease-associated repeats such as those responsible for SCA1  and Fragile X syndrome . In these cases, we simply retained both repeats and associated them with the disease.
We defined significant repeat length in the reference genome as any repeat with length within the top 5% of its class (corresponds to a percentile rank < 0.05 in Satellog). Using this cut-off, we determined whether the reference genome repeat length is significant for any of the disease-associated repeats within their respective disease classes. Interestingly, 80% (24/30) of the disease-associated repeats in Figure 1 were significantly long in the reference genome given their repeat class' length distribution (percentile rank < 0.05). In fact, 20 of 30 of all disease-associated repeats had a percentile rank of 0.01 or less indicating that these repeats were the extreme outliers within their class. Of the coding repeats, 12 of 17 had significant repeat lengths, including all the CAG-type repeats. Exceptions were the cleidocranial dysplasia (CCD), hand-foot-genital syndrome (HFGS), synpolydactyly, oculopharyngeal muscular dystrophy (OPMD), and holoprosencephaly coding GCG repeats. The CCCCGCCCCGCG dodecamer implicated in progressive myoclonic epilepsy type 1 (EPM1) is not included in this comparison because there were no other pure repeats of its class in the genome.
Polymorphic repeats detected in UniGene clusters
Disease-associated repeats detected in UniGene clusters
Unstable untranslated repeats organized by descending standard deviation Sample output from Satellog.
Of the 31 disease-associated repeats discussed previously, only 5 repeats were detected as polymorphic within UniGene clusters. We sought to understand why this occurred. Of the 31 disease-associated repeats, 4 failed to map within the genomic co-ordinates of any mapped UniGene cluster. The remaining 27 repeats mapped within a UniGene cluster's genomic co-ordinates. However, 16 of these failed to be detected within UniGene sequences even though they mapped within a UniGene cluster. This could be because of the 3' bias of the UniGene sequences, the incomplete nature of the clusters , sequence errors in the representative UniGene cluster sequence we searched against for hits (Hs.seq.uniq – see Methods for details), or the limitations of our mapping algorithm. Our approach enforces that the repeat must exist with at least 10 bp of flanking sequence, which leaves out repeats at the edge of UniGene clusters. The remaining 11 disease-associated repeats were detected within UniGene clusters, but only 5 of these repeats were polymorphic. On average, the repeats detected as polymorphic had more hits within UniGene clusters than those detected as stable (there were an average of 17.4 observations per repeat for the polymorphic repeats to 4.54 for stable repeats). This suggests that there is a greater chance of observing repeat polymorphism with deeper sampling. All of the polymorphic repeats were limited to one UniGene cluster and none of the lengths surpassed the disease pre-mutation threshold of 29, 25, 36, 42, and 39 pure repeats for the repeats responsible for increased prostate cancer risk (AIB-I), DRPLA, SBMA, SCA17, and FRAXE respectively .
Although one might expect greater polymorphism in UTR sequence relative to coding sequence due to reduced evolutionary constraints, both 5'-UTR and coding repeats had similar rates of polymorphism, whereas 3'-UTR repeats had significantly greater polymorphism compared to these two groups. This may be due to the documented 3'-UTR sequence over-representation in UniGene . However, depending on whether the repeat is within coding or UTR sequence, there appears to be constraints regarding what repeat unit sizes can tolerate large polymorphisms. Of the more polymorphic UTR repeats (those with sd values greater than 3), there was a single trinucleotide repeat amongst mainly dinucleotide and mononucleotide repeats (Figure 2, Table 2). On the other hand, the majority of coding repeat polymorphisms, although less pronounced, are almost entirely in factors of three (Figure 1, Table 1). Our results support the observation that coding microsatellite polymorphisms are usually in-frame in order to avoid a deleterious phenotype resulting from frame-shift or to provide a rapid evolutionary response to a changing environment .
It is important to consider that larger repeat polymorphisms could cause a UniGene cluster to "split" into two distinct clusters. This could downplay a repeat's polymorphism because such repeats would not be evaluated as a single group, therefore decreasing the repeat's sd value. This issue was addressed by pre-mapping all UniGene clusters to the human genome. If the repeat co-ordinates were within 10 kb of the UniGene genomic co-ordinates, then the repeat length hits was retained and merged into a single sd value. In practical terms this was not an issue, since only one of our most polymorphic repeats (sd > 2) mapped to two clusters.
There are certain limitations in using the GeneNote database to establish expression of repeat-containing genes. Specifically, the GeneNote microarray experiments were conducted with whole tissues, not tissues from particular tissue sub-types . For example, users limiting their search to repeats expressed in the brain must bear in mind the possibility that a transcript highly expressed in one anatomical region (i.e. hippocampus) may lack sufficient global expression to be detected in the whole brain tissue used by the GeneNote experiments. Users interested in expression in particular anatomical regions might benefit from integrating gene expression data from their anatomical region of interest with repeat data from Satellog.
Candidate repeats within the chromosome 22 schizophrenia linkage region.
Satellog enriches the current bioinformatics landscape in which repeats are viewed. For example, the GAA repeat in Friedreich's Ataxia  is not detected at all (chr9:67,109,320-67,109,339) in the UCSC genome browser  by the TRF  and Variable Number Tandem Repeats (VNTR) tracks. The VNTR feature in UCSC detects all perfect 2 to 10 repeat units with 10 or more copies. Repeats detected by this method may over-represent insignificant low period repeats and under-represent potentially interesting high period repeats. In Satellog, not only is the Friedreich's Ataxia GAA repeat detected, but its percentile rank also suggests that this size of GAA repeat is a relatively rare observation in the human genome (percentile rank = 0.045). Satellog integrates disparate data sources to give researchers an idea of how interesting certain repeats are based on their genetic location, tissue expression profile and polymorphism within UniGene. It should be noted that Satellog does not intend to be a de novo detection method for disease-associated repeats. Instead, it provides comprehensive, integrated bioinformatics platform to prioritize repeats in a convenient and efficient manner. Satellog also presents the first comprehensive identification and integration of disease-associated repeats with other genomic resources for use as bioinformatics reagents in other studies. Satellog should prove useful to investigators interested in prioritizing repeats for typing in diseases showing anticipation or in which repeat polymorphism is thought to play a role in etiology. In addition, given that all sequence information (i.e. the human genome sequence and UniGene sequences) is from presumed "normal" individuals lacking disease phenotypes; Satellog may also prove useful in extending our understanding of the normal role of repeats in genes and transcripts.
A perl script "repeatalyzer.pl" functions as a wrapper for a number of different programs to achieve the endpoints of Satellog. repeatalyzer.pl is run with perl v5.6.1 and used BioPerl v1.2 , the EnsEMBL Perl API (May 24th, 1999 release), MySQL v10.8 Distribution 3.23.21-beta (for pc-linux-gnu), BLAT v. 28  and v. 34 of the human genome sequence . This script was run in parallel on a 192 node linux cluster at the BCCA Genome Sciences Centre. More detailed methods information is available at http://satellog.bcgsc.ca.
Detecting microsatellite repeats with Tandem Repeats Finder (TRF)
We chose to detect sequences repeated at least twice and secondly, we were interested in exclusively pure repeat tracts which are more likely to expand following transmission [34–36]. Command-line TRF has seven parameters that can be manually assigned at run-time which include matching weight, mismatch and indel penalties, match probability, indel probability, minimum alignment score to report, and maximum period size to report . We found that matching weight, mismatch and indel penalties, minimum alignment score and maximum period size directly affected the length and purity of hits detected by TRF whereas changing the match and indel probability features was not useful. The match and indel probability features refer respectively to the percent identity and fraction of indels tolerated in each serial tandem unit detected as a hit. These features allow users to specify alternative expected matching and indel statistical distributions.
Next we evaluated the ability of the matching weight and maximum period size parameters to detect short repeats. Period size refers to the length of the tandemly repeated DNA unit, for instance CAG repeats have a period of 3. Since TRF hits must be at least 10 bp, the smallest hit for each repeat class reported in Satellog is 10 divided by the repeat unit length. For example, for CAG repeats, the smallest hit detectable that satisfies the minimum hit length is a 3 1/3 repeat unit hit (i.e. CAG CAG CAG C). In short, only pentanucleotide and larger repeats have a minimum of two repeat units in Satellog.
Lastly we investigated the utility of adjusting the mismatch and indel penalties. We found that setting the penalty for these parameters to 4090 produced no impure repeats as hits. TRF was run on whole chromosome FASTA files from v. 34 of the human genome downloaded from the UCSC genome browser. Hit purity was confirmed by visually inspecting the top high period hits (these hits have the highest probability of introducing indels due to the scoring scheme used by TRF .
Identifying unique repeat classes
A repeat can be represented in a number of ways in double-stranded DNA. TRF detects repeats by the first tandemly repeated unit, therefore, CAGCAGCAG, AGCAGCAGC, and GCAGCAGCA are detected as repeats of CAG, AGC, and GCA respectively. Furthermore, the reference human genome sequence is only presented as the positive strand. Repeats of GTC, TCG, and CGT on the positive strand represent 5'->3' CAG, AGC and GCA repeats respectively on the negative strand. Therefore, to identify all CAG repeats in the human genome it's necessary to detect all CAG, AGC, GCA, GTC, TCG, and CGT repeats on the positive strand. We developed an algorithm to generate all possible sequence varieties of a repeat unit on the positive and negative strands. Our repeat classification algorithm operates by taking an input repeat unit, i.e. CAG, removing the first letter (C in this case) and appending it to the end of the remainder (AG) to create the second repeat unit (AGC). This is then reverse complemented to generate the equivalent sequence on the negative strand (TCG). This procedure is repeated repeat unit length – 1 times to generate a unique identifier henceforth referred to as the repeat class. Each repeat in Satellog is associated with a single unique repeat class.
Preparing AffyMetrix expression data from the GeneNote database
The GeneNote (Gene Normal Tissue Expression) database provides baseline normal expression data of human genes for use in disease studies . GeneNote data is downloaded from the Gene Expression Omnibus (GEO). A total of twelve human tissue profiles are presented in GeneNote including bone marrow, brain, heart, kidney, liver, lung, pancreas, prostate, skeletal muscle, spinal cord, spleen, and thymus. These products were generated with the AffyMetrix HG-U95 A-E probe-set, covering 62,839 probe-sets. EnsEMBL genes have been mapped to AffyMetrix HG-U95 probes by the EnsEMBL project . Once a repeat is detected either inside or within 60 kb of an EnsEMBL gene, that gene's normal expression profile is evaluated by cross-referencing its AffyMetrix tags to the GeneNote database within Satellog.
Detecting repeat polymorphisms within UniGene clusters
UniGene contains the largest public repository of transcribed human sequence and represents an attempt to organize this wealth of expression data into discrete transcriptional loci . All human UniGene sequences were processed for use with repeatalyzer.pl. For each repeat detected in UTR or coding sequence, the repeat plus 10 bp of flanking sequence was extracted from EnsEMBL and queried using the BLAT algorithm  against a BLAT-formatted database created from sequences representing the longest, highest quality stretch of DNA from each individual UniGene cluster (pre-selected by UniGene as the file Hs.seq.uniq). Polymorphism is evaluated only if BLAT analysis against all UniGene clusters resulted in 1) hits that achieved BLAT scores at least 85% of the theoretical maximum for a perfect hit 2) 90% of the query sequence matched identically within the cluster 3) the repeat mapped within 10 kb of the genomic co-ordinates of the UniGene cluster. If a hit to a UniGene cluster satisfied these criteria, the length of the repeat in the cluster is stored in Satellog. This feature allows investigators to query all repeats with polymorphisms in UniGene clusters from genomic regions of interest.
Generating a measure of repeat length significance
After running the script to populate Satellog, each repeat's length is compared to the lengths of all repeats of the same repeat class. The majority of repeats associated with disease undergo expansions from already large reference genome lengths relative to other repeats of the same class . Each repeat's percentile rank is calculated from the distribution of repeat lengths within each repeat's class. It reflects the proportion of repeats with the same or greater length from the repeat class' genomic distribution.
Figure S1 – Repeat density (bp of repeat sequence / Mb) per human chromosome.
Available online at: http://satellog.bcgsc.ca/source.php.
Table S1 – Total repeat count and density by chromosome
Available online at: http://satellog.bcgsc.ca/source.php.
Table S2 – Repeat period count and density by chromosome
Available online at: http://satellog.bcgsc.ca/source.php.
Table S3 – Repeat unit count and density by chromosome
Available online at: http://satellog.bcgsc.ca/source.php.
Table S4 – Repeat unit count and density by gene region
Available online at: http://satellog.bcgsc.ca/source.php.
1) UBC/SFU CIHR Training Program for Bioinformatics in Health Research, Rooms 308/308A, 2206 East Mall, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada
2) Mark Mayo and Bernard Li at the BCCA Genome Sciences Centre for technical support with cluster computing.
3) Martin Krzywinski for creating the Satellog logo.
- Harper PS, Harley HG, Reardon W, Shaw DJ: Anticipation in myotonic dystrophy: new light on an old problem. Am J Hum Genet 1992, 51: 10–16.PubMed CentralPubMedGoogle Scholar
- Verkerk AJ, Pieretti M, Sutcliffe JS, Fu YH, Kuhl DP, Pizzuti A, Reiner O, Richards S, Victoria MF, Zhang FP, et al.: Identification of a gene (FMR-1) containing a CGG repeat coincident with a breakpoint cluster region exhibiting length variation in fragile X syndrome. Cell 1991, 65: 905–914. 10.1016/0092-8674(91)90397-HView ArticlePubMedGoogle Scholar
- Kremer EJ, Pritchard M, Lynch M, Yu S, Holman K, Baker E, Warren ST, Schlessinger D, Sutherland GR, Richards RI: Mapping of DNA instability at the fragile X to a trinucleotide repeat sequence p(CCG)n. Science 1991, 252: 1711–1714.View ArticlePubMedGoogle Scholar
- La Spada AR, Wilson EM, Lubahn DB, Harding AE, Fischbeck KH: Androgen receptor gene mutations in X-linked spinal and bulbar muscular atrophy. Nature 1991, 352: 77–79. 10.1038/352077a0View ArticlePubMedGoogle Scholar
- Cleary JD, Pearson CE: The contribution of cis-elements to disease-associated repeat instability: clinical and experimental evidence. Cytogenet Genome Res 2003, 100: 25–55. 10.1159/000072837View ArticlePubMedGoogle Scholar
- Koide R, Ikeuchi T, Onodera O, Tanaka H, Igarashi S, Endo K, Takahashi H, Kondo R, Ishikawa A, Hayashi T, et al.: Unstable expansion of CAG repeat in hereditary dentatorubral-pallidoluysian atrophy (DRPLA). Nat Genet 1994, 6: 9–13. 10.1038/ng0194-9View ArticlePubMedGoogle Scholar
- A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes. The Huntington's Disease Collaborative Research Group Cell 1993, 72: 971–983. 10.1016/0092-8674(93)90585-EGoogle Scholar
- Banfi S, Servadio A, Chung MY, Kwiatkowski TJJ, McCall AE, Duvick LA, Shen Y, Roth EJ, Orr HT, Zoghbi HY: Identification and characterization of the gene causing type 1 spinocerebellar ataxia. Nat Genet 1994, 7: 513–520. 10.1038/ng0894-513View ArticlePubMedGoogle Scholar
- Imbert G, Saudou F, Yvert G, Devys D, Trottier Y, Garnier JM, Weber C, Mandel JL, Cancel G, Abbas N, Durr A, Didierjean O, Stevanin G, Agid Y, Brice A: Cloning of the gene for spinocerebellar ataxia 2 reveals a locus with high sensitivity to expanded CAG/glutamine repeats. Nat Genet 1996, 14: 285–291. 10.1038/ng1196-285View ArticlePubMedGoogle Scholar
- Ikeda H, Yamaguchi M, Sugai S, Aze Y, Narumiya S, Kakizuka A: Expanded polyglutamine in the Machado-Joseph disease protein induces cell death in vitro and in vivo. Nat Genet 1996, 13: 196–202. 10.1038/ng0696-196View ArticlePubMedGoogle Scholar
- Zhuchenko O, Bailey J, Bonnen P, Ashizawa T, Stockton DW, Amos C, Dobyns WB, Subramony SH, Zoghbi HY, Lee CC: Autosomal dominant cerebellar ataxia (SCA6) associated with small polyglutamine expansions in the alpha 1A-voltage-dependent calcium channel. Nat Genet 1997, 15: 62–69. 10.1038/ng0197-62View ArticlePubMedGoogle Scholar
- David G, Abbas N, Stevanin G, Durr A, Yvert G, Cancel G, Weber C, Imbert G, Saudou F, Antoniou E, Drabkin H, Gemmill R, Giunti P, Benomar A, Wood N, Ruberg M, Agid Y, Mandel JL, Brice A: Cloning of the SCA7 gene reveals a highly unstable CAG repeat expansion. Nat Genet 1997, 17: 65–70. 10.1038/ng0997-65View ArticlePubMedGoogle Scholar
- Ross CA, Margolis RL, Becher MW, Wood JD, Engelender S, Cooper JK, Sharp AH: Pathogenesis of neurodegenerative diseases associated with expanded glutamine repeats: new answers, new questions. Prog Brain Res 1998, 117: 397–419.View ArticlePubMedGoogle Scholar
- Cummings CJ, Zoghbi HY: Trinucleotide repeats: mechanisms and pathophysiology. Annu Rev Genomics Hum Genet 2000, 1: 281–328. 10.1146/annurev.genom.1.1.281View ArticlePubMedGoogle Scholar
- Lalioti MD, Scott HS, Buresi C, Rossier C, Bottani A, Morris MA, Malafosse A, Antonarakis SE: Dodecamer repeat expansion in cystatin B gene in progressive myoclonus epilepsy. Nature 1997, 386: 847–851. 10.1038/386847a0View ArticlePubMedGoogle Scholar
- Matsuura T, Yamagata T, Burgess DL, Rasmussen A, Grewal RP, Watase K, Khajavi M, McCall AE, Davis CF, Zu L, Achari M, Pulst SM, Alonso E, Noebels JL, Nelson DL, Zoghbi HY, Ashizawa T: Large expansion of the ATTCT pentanucleotide repeat in spinocerebellar ataxia type 10. Nat Genet 2000, 26: 191–194. 10.1038/79911View ArticlePubMedGoogle Scholar
- Brook JD, McCurrach ME, Harley HG, Buckler AJ, Church D, Aburatani H, Hunter K, Stanton VP, Thirion JP, Hudson T, et al.: Molecular basis of myotonic dystrophy: expansion of a trinucleotide (CTG) repeat at the 3' end of a transcript encoding a protein kinase family member. Cell 1992, 68: 799–808. 10.1016/0092-8674(92)90154-5View ArticlePubMedGoogle Scholar
- Greco CM, Hagerman RJ, Tassone F, Chudley AE, Del Bigio MR, Jacquemont S, Leehey M, Hagerman PJ: Neuronal intranuclear inclusions in a new cerebellar tremor/ataxia syndrome among fragile X carriers. Brain 2002, 125: 1760–1771. 10.1093/brain/awf184View ArticlePubMedGoogle Scholar
- Jiang H, Mankodi A, Swanson MS, Moxley RT, Thornton CA: Myotonic dystrophy type 1 is associated with nuclear foci of mutant RNA, sequestration of muscleblind proteins and deregulated alternative splicing in neurons. Hum Mol Genet 2004, 13: 3079–3088. 10.1093/hmg/ddh327View ArticlePubMedGoogle Scholar
- Speer MC, Gilchrist JM, Stajich JM, Gaskell PC, Westbrook CA, Horrigan SK, Bartoloni L, Yamaoka LH, Scott WK, Pericak-Vance MA: Evidence for anticipation in autosomal dominant limb-girdle muscular dystrophy. J Med Genet 1998, 35: 305–308.PubMed CentralView ArticlePubMedGoogle Scholar
- Bayless TM, Picco MF, LaBuda MC: Genetic anticipation in Crohn's disease. Am J Gastroenterol 1998, 93: 2322–2325. 10.1111/j.1572-0241.1998.02322.xView ArticlePubMedGoogle Scholar
- Horwitz M, Goode EL, Jarvik GP: Anticipation in familial leukemia. Am J Hum Genet 1996, 59: 990–998.PubMed CentralPubMedGoogle Scholar
- Wright GD, Regan M, Deighton CM, Wallis G, Doherty M: Evidence for genetic anticipation in nodal osteoarthritis. Ann Rheum Dis 1998, 57: 524–526.PubMed CentralView ArticlePubMedGoogle Scholar
- Bonifati V, Vanacore N, Meco G: Anticipation of onset age in familial Parkinson's disease. Neurology 1994, 44: 1978–1979.View ArticlePubMedGoogle Scholar
- McDermott E, Khan MA, Deighton C: Further evidence for genetic anticipation in familial rheumatoid arthritis. Ann Rheum Dis 1996, 55: 475–477.PubMed CentralView ArticlePubMedGoogle Scholar
- Bleyl S, Nelson L, Odelberg SJ, Ruttenberg HD, Otterud B, Leppert M, Ward K: A gene for familial total anomalous pulmonary venous return maps to chromosome 4p13-q12. Am J Hum Genet 1995, 56: 408–415.PubMed CentralPubMedGoogle Scholar
- Ohara K, Suzuki Y, Ushimi Y, Yoshida K: Anticipation and imprinting in Japanese familial mood disorders. Psychiatry Res 1998, 79: 191–198. 10.1016/S0165-1781(98)00045-6View ArticlePubMedGoogle Scholar
- Bassett AS, Honer WG: Evidence for anticipation in schizophrenia. Am J Hum Genet 1994, 54: 864–870.PubMed CentralPubMedGoogle Scholar
- Bassett AS, Husted J: Anticipation or ascertainment bias in schizophrenia? Penrose's familial mental illness sample. Am J Hum Genet 1997, 60: 630–637.PubMed CentralPubMedGoogle Scholar
- Battaglia M, Bertella S, Bajo S, Binaghi F, Bellodi L: Anticipation of age at onset in panic disorder. Am J Psychiatry 1998, 155: 590–595.View ArticlePubMedGoogle Scholar
- Ohara K, Suzuki Y, Ochiai M, Yoshida K: Age of onset anticipation in anxiety disorders. Psychiatry Res 1999, 89: 215–221. 10.1016/S0165-1781(99)00106-7View ArticlePubMedGoogle Scholar
- Benson G: Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 1999, 27: 573–580. 10.1093/nar/27.2.573PubMed CentralView ArticlePubMedGoogle Scholar
- Kent WJ, Sugnet CW, Furey TS, Roskin KM, Pringle TH, Zahler AM, Haussler D: The human genome browser at UCSC. Genome Res 2002, 12: 996–1006. 10.1101/gr.229102. Article published online before print in May 2002PubMed CentralView ArticlePubMedGoogle Scholar
- Chong SS, McCall AE, Cota J, Subramony SH, Orr HT, Hughes MR, Zoghbi HY: Gametic and somatic tissue-specific heterogeneity of the expanded SCA1 CAG repeat in spinocerebellar ataxia type 1. Nat Genet 1995, 10: 344–350. 10.1038/ng0795-344View ArticlePubMedGoogle Scholar
- Chung MY, Ranum LP, Duvick LA, Servadio A, Zoghbi HY, Orr HT: Evidence for a mechanism predisposing to intergenerational CAG repeat instability in spinocerebellar ataxia type I. Nat Genet 1993, 5: 254–258. 10.1038/ng1193-254View ArticlePubMedGoogle Scholar
- Kunst CB, Warren ST: Cryptic and polar variation of the fragile X repeat could result in predisposing normal alleles. Cell 1994, 77: 853–861. 10.1016/0092-8674(94)90134-1View ArticlePubMedGoogle Scholar
- Campuzano V, Montermini L, Molto MD, Pianese L, Cossee M, Cavalcanti F, Monros E, Rodius F, Duclos F, Monticelli A, Zara F, Canizares J, Koutuikova H, Bidichandani SI, Gellera C, Brice A, Trouillas P, De Michele G, Filla A, De Frutos R, Palau F, Patel PI, Di Donato S, Mandel JL, Cocozza S, Koenig M, Pandolfo M: Friedreich's ataxia: autosomal recessive disease caused by an intronic GAA triplet repeat expansion. Science 1996, 271: 1423–1427.View ArticlePubMedGoogle Scholar
- Subramanian S, Madgula VM, George R, Mishra RK, Pandit MW, Kumar CS, Singh L: MRD: a microsatellite repeats database for prokaryotic and eukaryotic genomes. Genome Biol 2002, 3: PREPRINT0011. 10.1186/gb-2002-3-12-preprint0011View ArticlePubMedGoogle Scholar
- Collins JR, Stephens RM, Gold B, Long B, Dean M, Burt SK: An exhaustive DNA micro-satellite map of the human genome using high performance computing. Genomics 2003, 82: 10–19. 10.1016/S0888-7543(03)00076-4View ArticlePubMedGoogle Scholar
- Wheeler DL, Church DM, Edgar R, Federhen S, Helmberg W, Madden TL, Pontius JU, Schuler GD, Schriml LM, Sequeira E, Suzek TO, Tatusova TA, Wagner L: Database resources of the National Center for Biotechnology Information: update. Nucleic Acids Res 2004, 32 Database issue: D35–40. 10.1093/nar/gkh073View ArticleGoogle Scholar
- Hubbard T, Barker D, Birney E, Cameron G, Chen Y, Clark L, Cox T, Cuff J, Curwen V, Down T, Durbin R, Eyras E, Gilbert J, Hammond M, Huminiecki L, Kasprzyk A, Lehvaslaiho H, Lijnzaad P, Melsopp C, Mongin E, Pettett R, Pocock M, Potter S, Rust A, Schmidt E, Searle S, Slater G, Smith J, Spooner W, Stabenau A, Stalker J, Stupka E, Ureta-Vidal A, Vastrik I, Clamp M: The Ensembl genome database project. Nucleic Acids Res 2002, 30: 38–41. 10.1093/nar/30.1.38PubMed CentralView ArticlePubMedGoogle Scholar
- Shmueli O, Horn-Saban S, Chalifa-Caspi V, Shmoish M, Ophir R, Benjamin-Rodrig H, Safran M, Domany E, Lancet D: GeneNote: whole genome expression profiles in normal human tissues. C R Biol 2003, 326: 1067–1072.View ArticlePubMedGoogle Scholar
- Subramanian S, Mishra RK, Singh L: Genome-wide analysis of microsatellite repeats in humans: their abundance and density in specific genomic regions. Genome Biol 2003, 4: R13. 10.1186/gb-2003-4-2-r13PubMed CentralView ArticlePubMedGoogle Scholar
- Crisponi L, Deiana M, Loi A, Chiappe F, Uda M, Amati P, Bisceglia L, Zelante L, Nagaraja R, Porcu S, Ristaldi MS, Marzella R, Rocchi M, Nicolino M, Lienhardt-Roussie A, Nivelon A, Verloes A, Schlessinger D, Gasparini P, Bonneau D, Cao A, Pilia G: The putative forkhead transcription factor FOXL2 is mutated in blepharophimosis/ptosis/epicanthus inversus syndrome. Nat Genet 2001, 27: 159–166. 10.1038/84781View ArticlePubMedGoogle Scholar
- Clark RM, Dalgliesh GL, Endres D, Gomez M, Taylor J, Bidichandani SI: Expansion of GAA triplet repeats in the human genome: unique origin of the FRDA mutation at the center of an Alu. Genomics 2004, 83: 373–383. 10.1016/j.ygeno.2003.09.001View ArticlePubMedGoogle Scholar
- Kashi Y, King D, Soller M: Simple sequence repeats as a source of quantitative genetic variation. Trends Genet 1997, 13: 74–78. 10.1016/S0168-9525(97)01008-1View ArticlePubMedGoogle Scholar
- MacDonald ME, Novelletto A, Lin C, Tagle D, Barnes G, Bates G, Taylor S, Allitto B, Altherr M, Myers R, Lehrach H, Collins FS, Wasmuth JJ, Frontali M, Gusella JF: The Huntington's disease candidate region exhibits many different haplotypes. Nat Genet 1992, 1: 99–103. 10.1038/ng0592-99View ArticlePubMedGoogle Scholar
- Shaw SH, Kelly M, Smith AB, Shields G, Hopkins PJ, Loftus J, Laval SH, Vita A, De Hert M, Cardon LR, Crow TJ, Sherrington R, DeLisi LE: A genome-wide search for schizophrenia susceptibility genes. Am J Med Genet 1998, 81: 364–376. 10.1002/(SICI)1096-8628(19980907)81:5<364::AID-AJMG4>3.0.CO;2-TView ArticlePubMedGoogle Scholar
- Pulver AE, Karayiorgou M, Wolyniec PS, Lasseter VK, Kasch L, Nestadt G, Antonarakis S, Housman D, Kazazian HH, Meyers D, Ott J, Lamacz M, Liang K-Y, Hanfelt J, Ullrich G, DeMarchi N, Ranu E, McHugh PR, Adler L, Thomas M: Sequential strategy to identify a susceptibility gene for schizophrenia: report of potential linkage on chromosome 22q12-q13.1: Part 1. Am J Med Genet 1994, 54: 36–43. 10.1002/ajmg.1320540108View ArticlePubMedGoogle Scholar
- Coon H, Jensen S, Holik J, Hoff M, Myles-Worsley M, Reimherr F, Wender P, Waldo M, Freedman R, Leppert M, et al.: Genomic scan for genes predisposing to schizophrenia. Am J Med Genet 1994, 54: 59–71. 10.1002/ajmg.1320540111View ArticlePubMedGoogle Scholar
- Murphy KC: Schizophrenia and velo-cardio-facial syndrome. Lancet 2002, 359: 426–430. 10.1016/S0140-6736(02)07604-3View ArticlePubMedGoogle Scholar
- Stajich JE, Block D, Boulez K, Brenner SE, Chervitz SA, Dagdigian C, Fuellen G, Gilbert JG, Korf I, Lapp H, Lehvaslaiho H, Matsalla C, Mungall CJ, Osborne BI, Pocock MR, Schattner P, Senger M, Stein LD, Stupka E, Wilkinson MD, Birney E: The Bioperl toolkit: Perl modules for the life sciences. Genome Res 2002, 12: 1611–1618. 10.1101/gr.361602PubMed CentralView ArticlePubMedGoogle Scholar
- Kent WJ: BLAT--the BLAST-like alignment tool. Genome Res 2002, 12: 656–664. 10.1101/gr.229202. Article published online before March 2002PubMed CentralView ArticlePubMedGoogle Scholar
- Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W, Funke R, Gage D, Harris K, Heaford A, Howland J, Kann L, Lehoczky J, LeVine R, McEwan P, McKernan K, Meldrim J, Mesirov JP, Miranda C, Morris W, Naylor J, Raymond C, Rosetti M, Santos R, Sheridan A, Sougnez C, Stange-Thomann N, Stojanovic N, Subramanian A, Wyman D, Rogers J, Sulston J, Ainscough R, Beck S, Bentley D, Burton J, Clee C, Carter N, Coulson A, Deadman R, Deloukas P, Dunham A, Dunham I, Durbin R, French L, Grafham D, Gregory S, Hubbard T, Humphray S, Hunt A, Jones M, Lloyd C, McMurray A, Matthews L, Mercer S, Milne S, Mullikin JC, Mungall A, Plumb R, Ross M, Shownkeen R, Sims S, Waterston RH, Wilson RK, Hillier LW, McPherson JD, Marra MA, Mardis ER, Fulton LA, Chinwalla AT, Pepin KH, Gish WR, Chissoe SL, Wendl MC, Delehaunty KD, Miner TL, Delehaunty A, Kramer JB, Cook LL, Fulton RS, Johnson DL, Minx PJ, Clifton SW, Hawkins T, Branscomb E, Predki P, Richardson P, Wenning S, Slezak T, Doggett N, Cheng JF, Olsen A, Lucas S, Elkin C, Uberbacher E, Frazier M, Gibbs RA, Muzny DM, Scherer SE, Bouck JB, Sodergren EJ, Worley KC, Rives CM, Gorrell JH, Metzker ML, Naylor SL, Kucherlapati RS, Nelson DL, Weinstock GM, Sakaki Y, Fujiyama A, Hattori M, Yada T, Toyoda A, Itoh T, Kawagoe C, Watanabe H, Totoki Y, Taylor T, Weissenbach J, Heilig R, Saurin W, Artiguenave F, Brottier P, Bruls T, Pelletier E, Robert C, Wincker P, Smith DR, Doucette-Stamm L, Rubenfield M, Weinstock K, Lee HM, Dubois J, Rosenthal A, Platzer M, Nyakatura G, Taudien S, Rump A, Yang H, Yu J, Wang J, Huang G, Gu J, Hood L, Rowen L, Madan A, Qin S, Davis RW, Federspiel NA, Abola AP, Proctor MJ, Myers RM, Schmutz J, Dickson M, Grimwood J, Cox DR, Olson MV, Kaul R, Shimizu N, Kawasaki K, Minoshima S, Evans GA, Athanasiou M, Schultz R, Roe BA, Chen F, Pan H, Ramser J, Lehrach H, Reinhardt R, McCombie WR, de la Bastide M, Dedhia N, Blocker H, Hornischer K, Nordsiek G, Agarwala R, Aravind L, Bailey JA, Bateman A, Batzoglou S, Birney E, Bork P, Brown DG, Burge CB, Cerutti L, Chen HC, Church D, Clamp M, Copley RR, Doerks T, Eddy SR, Eichler EE, Furey TS, Galagan J, Gilbert JG, Harmon C, Hayashizaki Y, Haussler D, Hermjakob H, Hokamp K, Jang W, Johnson LS, Jones TA, Kasif S, Kaspryzk A, Kennedy S, Kent WJ, Kitts P, Koonin EV, Korf I, Kulp D, Lancet D, Lowe TM, McLysaght A, Mikkelsen T, Moran JV, Mulder N, Pollara VJ, Ponting CP, Schuler G, Schultz J, Slater G, Smit AF, Stupka E, Szustakowski J, Thierry-Mieg D, Thierry-Mieg J, Wagner L, Wallis J, Wheeler R, Williams A, Wolf YI, Wolfe KH, Yang SP, Yeh RF, Collins F, Guyer MS, Peterson J, Felsenfeld A, Wetterstrand KA, Patrinos A, Morgan MJ, Szustakowki J, de Jong P, Catanese JJ, Osoegawa K, Shizuya H, Choi S, Chen YJ: Initial sequencing and analysis of the human genome. Nature 2001, 409: 860–921. 10.1038/35057062View ArticlePubMedGoogle Scholar
- Berman HM, Battistuz T, Bhat TN, Bluhm WF, Bourne PE, Burkhardt K, Feng Z, Gilliland GL, Iype L, Jain S, Fagan P, Marvin J, Padilla D, Ravichandran V, Schneider B, Thanki N, Weissig H, Westbrook JD, Zardecki C: The Protein Data Bank. Acta Crystallogr D Biol Crystallogr 2002, 58: 899–907. 10.1107/S0907444902003451View ArticlePubMedGoogle Scholar
- Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, Harris MA, Hill DP, Issel-Tarver L, Kasarskis A, Lewis S, Matese JC, Richardson JE, Ringwald M, Rubin GM, Sherlock G: Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 2000, 25: 25–29. 10.1038/75556PubMed CentralView ArticlePubMedGoogle Scholar
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