- Open Access
A universal genomic coordinate translator for comparative genomics
BMC Bioinformatics volume 15, Article number: 227 (2014)
Genomic duplications constitute major events in the evolution of species, allowing paralogous copies of genes to take on fine-tuned biological roles. Unambiguously identifying the orthology relationship between copies across multiple genomes can be resolved by synteny, i.e. the conserved order of genomic sequences. However, a comprehensive analysis of duplication events and their contributions to evolution would require all-to-all genome alignments, which increases at N2 with the number of available genomes, N.
Here, we introduce Kraken, software that omits the all-to-all requirement by recursively traversing a graph of pairwise alignments and dynamically re-computing orthology. Kraken scales linearly with the number of targeted genomes, N, which allows for including large numbers of genomes in analyses. We first evaluated the method on the set of 12 Drosophila genomes, finding that orthologous correspondence computed indirectly through a graph of multiple synteny maps comes at minimal cost in terms of sensitivity, but reduces overall computational runtime by an order of magnitude. We then used the method on three well-annotated mammalian genomes, human, mouse, and rat, and show that up to 93% of protein coding transcripts have unambiguous pairwise orthologous relationships across the genomes. On a nucleotide level, 70 to 83% of exons match exactly at both splice junctions, and up to 97% on at least one junction. We last applied Kraken to an RNA-sequencing dataset from multiple vertebrates and diverse tissues, where we confirmed that brain-specific gene family members, i.e. one-to-many or many-to-many homologs, are more highly correlated across species than single-copy (i.e. one-to-one homologous) genes. Not limited to protein coding genes, Kraken also identifies thousands of newly identified transcribed loci, likely non-coding RNAs that are consistently transcribed in human, chimpanzee and gorilla, and maintain significant correlation of expression levels across species.
Kraken is a computational genome coordinate translator that facilitates cross-species comparisons, distinguishes orthologs from paralogs, and does not require costly all-to-all whole genome mappings. Kraken is freely available under LPGL from http://github.com/nedaz/kraken.
An organism’s genome contains a collection of genes and regulatory elements that are located in particular order and orientation. The organization and relative distance of these features from one another can direct their activity patterns. A classic example includes the Hox gene clusters [1, 2], which are preserved as four to eight distinct regions among vertebrates . The Hox proteins and their regulatory machinery control organism development, and while the different copies share sequence similarity with each other, it is the spatial organization that determines the timing of expression. The Hox genes have undergone several duplication and expansion events during evolutionary history, allowing for more specialized roles in fine-tuning development, facilitating increased organismal complexity . More generally, gene duplications are a common mechanism for subsequent sub- and neo-functionalization, both in terms of proteins, as well as in regulation. For example, up to two thirds of vertebrate genes are members of families , which have been generated by local duplications, block duplications and whole genome duplications.
For a comprehensive comparative genomics study, it is thus paramount to accurately identify orthologous sequences that arose through duplication events, both prior to and after speciation. Due to complex patterns of selective pressure, resulting in conservation of sequences, loss of sequences, as well as invention of new functions, nucleotide or protein similarity is not a reliable measure to unambiguously resolve orthologs . However, orthologs can be recognized through conserved synteny, i.e. the locally conserved order and orientation of features, which has been observed even in species with high turnover rates of gene duplication, expansion and loss, such as in mammals . Synteny alignments are either computed relative to one central genome, as for example human, as described in the 29 mammalian genomes project , or via a complete set of pairwise comparisons, where the computational time for the analysis of N genomes is in the order of O(N2). Here, we describe a novel computational method, Kraken, which provides both the independence of a central genome, as well as eliminating the time consuming step of generating all pairwise synteny maps. For setting up a synteny framework, Kraken uses maps generated by the genome-wide synteny alignment programs and methods such as LASTZ chained alignments , Mummer , SyMAP , Satsuma , SynMap , or alignment graphs, for example Enredo/Pecan , and HAL . Next, Kraken infers indirect syntenic relationships between two genomes not connected through a synteny map via indirection, i.e. the mapping of regions through a graph of synteny maps and by dynamically augmenting local alignments.
As such, Kraken constitutes a core utility aiming at resolving several bioinformatics challenges currently facing life sciences. In particular, high-throughput sequencing technologies generate millions of reads from RNA, which, in turn, predict tens or hundreds of thousands of transcribed loci, each of which can contain multiple isoforms. To assess the quality and biological relevance of each prediction, additional evidence is required. An automated utility, which compares transcriptional activity in orthologous regions across multiple species, can provide such evidence, in particular for novel loci that might have a function that is yet to be determined.
In the following sections, we describe Kraken’s implementation, and evaluation of the accuracy, sensitivity, and specificity on a set of 12 Drosophila genomes spanning a long range of genomic distances  and the well-annotated mouse, human, and rat genomes. We then apply Kraken to rapidly and automatically establish transcription orthology maps across multiple vertebrate species for known and novel loci, leveraging previously generated RNA-Sequence data .
An overview of Kraken’s workflow is shown in Figure 1a: the input is comprised of (i) genome sequences, in chromosome or scaffold coordinates; (ii) synteny maps, generated from synteny alignment programs or extracted from multiple alignment graphs; and (iii) annotations or feature coordinates, specified in Gene Transfer Format (GTF). For each genome, Kraken’s output is provided on two levels: (i) GTF files in translated coordinates, preserving all of the original information; and, if applicable, (ii) a list of spatial relationships between overlapping translated annotations and native annotations. Figure 1b shows a flow chart of how the input data is processed. After all genomes, synteny maps, and input coordinates are loaded into memory, each query coordinate is translated into the genomic coordinate system of the target genome in a multi-step process: (i) estimate candidate locations of orthologous coordinates through the synteny graph; (ii) perform a rapid alignment of the input sequence against the target region based on a cross-correlation algorithm; and (iii) compute a local sequence alignment to determine the exact target coordinates. Optionally, (iv) coordinates in target coordinates are compared against a reference GTF, accommodating for features with multi-exonic structures and multiple isoforms. In the following, we describe each step in detail.
Kraken reads the meta-data accompanying a dataset from a configuration file. This file lists the file locations of the genomes (FASTA format), the synteny maps (LASTZ general format and Satsuma format, Kraken provides conversion tools for other formats) and the genomes they connect and in what direction. Kraken requires the pre-computed pairwise synteny maps to provide the coordinates of orthologous regions (i.e. not the synteny alignments), in intervals specifying the corresponding: (i) chromosome or scaffold name; (ii) first nucleotide position in the interval; (iii) last nucleotide position in the interval. Maps need to be provided in one direction (genome A → genome B) only, and are duplicated and inverted in memory to supply both directions (genome B → genome A). All positions (start and stop) in the synteny maps are sorted by chromosome first and position next in target coordinates, setting up a data structure for binary search.
Kraken performs an exhaustive search through all possibilities from source to target genomes through a synteny graph built from pairwise synteny as specified in the configuration file, and selects the path with either (a) the lowest number of indirect mappings, or (b) by minimizing the accumulated genomic distances, if specified. The path is determined prior to coordinate translation and fixed from there on. More formally, the synteny graph G(V,E) is an undirected graph, where the nodes, V(G), consist of the genomes and the pairwise synteny alignments between the genomes constitute the edges of the graph, E(G). The graph edges are weighted by genomic distances if available, and otherwise they are non-weighted. For a given pair of genomes, (u, v), the shortest path is found by minimizing the edge distances on the path.
Each interval specified in the source GTF is translated individually by looking up the lower bound of the start and higher bound of the end position. The coordinate start and stop can either directly fall into syntenic anchors, or in between anchors, in which case the candidate interval is widened to the next adjacent syntenic map entries. This process is repeated until the target genome is reached. Translated target coordinates on the same chromosome or scaffold with syntenic flanks in consistent orientation of up to 100,000 nt are passed on to the next step, otherwise, the region is split into two target intervals, one from each side (50,000 nt each) to allow for searching the boundaries of syntenic breaks.
For computational efficiency, Kraken performs a quick search of the source sequence against the target interval by employing an approximate cross-correlation alignment, as originally implemented by Satsuma . To limit the processing time and memory requirements of the underlying Fourier Transform, the target interval is broken into overlapping sequences of 214 nucleotides in size, the source sequence is cross-correlated against each block, and the block with the highest absolute cross-correlation signal is computed. Based on the size of the source interval, Kraken determines a candidate region equal to that size plus flanks on each end (+/− 12 nt per default) based on the offset of the cross-correlation signal.
Dynamic local re-alignment
Detailed alignment of the source sequence is performed using the Cola  implementation of a banded alignment with gap-affine penalties [21, 22] against the target sub-sequence, which is defined by the offset determined by the rapid alignment. For source intervals of >100 nt, the sequence is split into two 100 nt chunks at each end covering the start and end region of the sequence, both in the source and the target genomes. A p-value threshold to accept alignments is configurable and defaults to 10−4. Alignments are not required to cover the entire source sequence, i.e. nucleotide mismatches at the boundaries are permissible (as forcing alignments would infer the risk of false insertions). In that case, the final translated target coordinates are estimated based on the alignment offset into the source sequence, i.e. if the alignment starts at position k in the source feature, the target start coordinate is adjusted by -k nucleotides (and vice versa for the final stop coordinate). Output is provided in GTF format, where for all the items that were successfully translated an output entry is produced containing the translated coordinates.
Optionally, Kraken allows for directly comparing translated coordinates to features specified in the coordinates of the target genome, following the GTF file convention for exons, transcripts, and genes. Kraken stores GTF coordinates internally in the following data structures (classes): (i) exons, which are intervals (implemented as annotation items), consisting of a chromosome name, start and end coordinate; (ii) transcripts, which are generalizations of items and extend functionality by owning a number of exons; and (iii) loci, which also generalize items but own a set of transcripts. Kraken instantiates arrays of each type in sorted order for efficient retrieval, and stores ownership relationships between items, transcripts and loci bi-directionally to facilitate fast referencing in either direction. Thus, Kraken allows for rapidly inferring spatial relationships between the genomic features in the source and target genome, if the latter are available in GTF format, and taking into account their multi-exonic structures. Kraken classifies matches as: (i) full sense overlap, i.e. all exons of the source transcript overlap all exons of the target transcript and fall on the same strand; (ii) partial sense overlap, i.e. one or more exon overlap in sense direction; (iii) intronic (sense or antisense), i.e. the coordinates of the source transcript overlap an intron of a target locus; and (iv) antisense (full or partial), i.e. overlapping target exons in the opposite strand. The coordinates of all translated features, the relationships described above, and the overlapping target annotations are reported in human-readable outputs that are also friendly to machine parsers.
Results and discussion
Translating genomic intervals between 12 fruit fly species: evaluating synteny graphs
To examine how translation through intermediate synteny maps along a graph impacts sensitivity, we evaluated Kraken on 264 pairwise comparisons between the genomes of 12 Drosophila fruit flies . Drosophila species are a diverse group (Figure 2a) and cover small to large genomic timespans (Figure 2b), allowing for measuring accuracy as a function of genomic distance. Moreover, the genomes are of moderate size (~150 Mb), so that computing a full set of pairwise synteny maps as the baseline for comparison is computationally feasible. We first generated all pairwise genome-wide syntenic alignments using Satsuma . We next selected 75,000 random genomic intervals of 200 base pairs in size for each genome, and used Kraken to translate these sequences into the coordinates of all other genomes using three different topologies: (i) a star configuration with D.melanogaster in the center (Figure 2c); (ii) a star configuration with D.sechillia in center (Figure 2d); and (iii) a clade configuration (Figure 2e) roughly following species phylogeny (Figure 2a). Table 1 summarizes the results. We first observed that for closely related species, such as D.yakuba and D.erecta, more than 80% of blocks could be successfully translated, while this fraction drops to about 12% for more distantly related species such as D.simulans and D.wilstoni. However, genomic distance does not majorly impact the fraction of successful indirect to direct translations: for example, for the closely related species pair D.erecta/D.yakuba in the melanogaster star configuration, 97.9% of directly translated blocks are matched in the indirect translation, while this fraction is only slightly lower for the more distantly related pairs D.erecta/D.mojavensis (96.9%) and D.erecta/D.willstoni (95.5%). The median fraction of identical indirect/direct translations is highest in the D.melanogaster star topology (Figure 2c), followed by the D.sechellia star (Figure 2d), and clade (Figure 2d) topologies. While overall, using the high-quality genome of D.melanogaster as the center yields the best results with respect to the topologies evaluated here, individual statistics suggest that a more complex topology allowing for alternative paths to traverse the synteny graph yields higher sensitivity (Table 1).
Matching genes between human, rat, and mouse: a quantitative assessment
We evaluated Kraken’s quantitative performance with regards to known genomic features by matching translated gene structures across three well-annotated high-quality mammalian genomes: human (hg19), mouse (mm10), and rat (rn5). Using pairwise synteny maps between the genomes generated by LASTZ , we translated all annotated genes (Ensembl 68) in six pairwise comparisons across genomic coordinates. Table 2 summarizes the results: overall, between 77% and 95% of transcripts could be unambiguously translated, with between 90% and 96% of these overlapping with annotated loci in the target genome. This fraction is higher for the protein-coding set, ranging from 93% to 98%, likely due to higher sequence conservation and sequence similarity. Kraken matched more annotated transcripts between human and mouse than human and rat, possibly because of differences in the quality of the genome builds and/or annotation. Among the 1,760 protein coding gene loci that failed to be translated from human to mouse, more than half (926) can be divided into the following five categories: (i) 483 uncharacterized and hypothetical proteins; (ii) 188 loci with predicted open reading frames but without functional prediction; (iii) 113 pseudogenes from ribosomal proteins; (iv) 56 zinc finger proteins; (v) 51 olfactory receptors; and (vi) 35 keratin associated proteins. While the first two categories represent genes of uncertain annotation status, the latter are comprised of members of gene families that are highly variable in copy number, or genes that have been known to undergo lineage-specific expansions. Figure 3 shows three-way Venn diagrams based on maximal overlaps (i.e. one-to-one relationships between transcripts in different genomes) for all transcripts (Figure 3a), and for the protein-coding subsets (Figure 3b). As in the pairwise comparisons, the fraction of overlaps is higher for protein coding genes. Notably, the rat annotates the lowest number of isoforms per protein coding gene, however, more than 90% of those overlap isoforms in both human and mouse, and almost all overlap mouse transcripts, suggesting that the rat annotation mostly contains dominant isoforms. We attribute the large fraction of ‘human-only’ transcripts to the more than three-fold number of annotated alternative isoforms per human gene locus.
We next compared the results to the methods HalLiftover  and RATT , using the Ensembl Compara database , a manually curated set of orthologous relationships between protein-coding transcripts across species for human, as independent metrics (Table 3). All methods largely agree in their predictions, with Kraken consistently and on all data sets exhibiting the highest overlap with ComparaDB, as well as the lowest number of transcripts predicted by ComparaDB only (Table 3). We note that RATT, which we ran in ‘species mode’ , yielded similar results to HalLiftover and Kraken on the mouse/rat comparison, but considerably fewer predictions for the more distantly related human/mouse and human/rat data sets.
Accuracy on nucleotide level: a qualitative assessment
We next examined the accuracy of Kraken, comparing the translated boundaries of exons to gene models native to the target genome for the human, mouse, and rat dataset (Table 4). For protein coding exons, Kraken matches 70 to 83% of complete exons between genomes with exact agreement at both splice junctions, and up to 97% of exon with exact matches in at least one splice junction. In examining cases in which the predictions did not exactly match the annotations, we found that the majority of differences differ by multiples of three nucleotides (Figure 4a), which is consistent with varying numbers of amino acids across species, indicating that the predicted coordinates could reflect actual biological differences in transcription between species. By contrast, we did not observe this pattern for exons of long intergenic non-coding genes (Figure 4b), which further supports that the periodicity observed for protein coding genes is biologically valid, rather than stemming from algorithmic artifacts, such as systematic alignment biases.
Resolving transcribed single-copy genes and gene family orthologs in vertebrates
In order to illustrate Kraken’s power as a practical tool for processing large and complex datasets, we re-analyzed publicly available RNA-Sequence data obtained from different vertebrates and multiple tissues . We first mapped the RNA-Sequence reads onto the respective genomes of human, chimp, gorilla, opossum, and chicken using Tophat , without reference annotation guidance, and allowing for the detection of un-annotated transcripts and isoforms. Next, we estimated RPKM expression values from the unpaired reads using Cufflinks/Cuffdiff  and merged experimentally found transcripts with the reference annotations (Ensembl 64) using Cuffmerge . We then used Kraken to translate the coordinates of transcribed features through a minimal set of LASTZ-chained alignments. A selection of pairwise interspecies transcript relationships are summarized in Table 5: overall, the number of transcripts that can be translated decreases with genomic distance from 250,000 for human-chimp to 100,000 for chicken-human. Likewise, the fraction of protein-coding genes, which are among the most highly conserved genomic features, is higher for more distantly related organisms. We next investigated whether protein-coding single copy genes and gene family members, defined by Ensembl  based on clustering by protein similarity, consistently show distinct expression patterns across tissues and species (we excluded genes with RPKM values < 1). We computed Spearman’s correlation coefficients between the tissues of all species-pairs separately for the two gene sets, and compared them to each other, highlighting the differences (Figure 5): in all comparisons of mismatched tissues (e.g. brain versus liver), single copy genes are more correlated than orthologous gene family members (light grey dots, Figure 5). Moreover, gene family paralogs are more highly correlated in matched brain and cerebellum tissues compared to single copy genes (black dots, Figure 5), while liver and kidney show the opposite trend (green and blue dots, Figure 5), with heart showing patterns in between (pink dots, Figure 5). In all cases, testis is least correlated compared with other tissues (yellow dots, Figure 5) both in single copy genes and gene families, and for large genomic distances (chicken-human, chicken-opossum, opossum-human). Testis genes are also less correlated in cross-species comparisons, while correlation is comparable to other tissues in primates (red dots, Figure 5). In terms of absolute values for correlations of both single copy and multiple copy genes, species are grouped according to phylogeny, including the primate clade, albeit statistically weakly (p < 0.086), with 10 out of 15 matched tissue comparisons (including male/female pairs) placing human closer to chimp than gorilla. Notably, comparisons involving kidney indicate higher correlations for human-gorilla than human-chimp.
Expression of novel transcripts in three primates
Kraken’s independence of known annotations or protein coding genes allows for the analysis of transcribed loci that have not previously been characterized. For example, thousands of long intergenic non-coding RNAs have recently been found in human , mouse , zebrafish , and dog . In the human genome, we found a total of 67,580 actively transcribed loci based on RNA-Seq data , 17,421 of which fell outside of Ensembl annotations. Of these, 6,281 and 5,968 were also actively transcribed in chimpanzee and gorilla respectively, with 3,503 transcribed in all three genomes. While the expression levels are correlated across species at much lower levels than those of known protein-coding genes, matched tissue comparisons still exhibit statistically significant correlations (Figure 6), with testis being the most correlated tissue in both human-chimp and human-gorilla. The reported correlation measures have high statistical significance with a maximum p-value of 10−8.
The analytical power of comparing features across multiple genomes has been demonstrated in the past and dates back to the early days of modern genomics. More recent examples, in which the order and orientation of genomic landmarks played a critical role, include describing a whole genome duplication event in the baker’s yeast Saccharomyces cerevisiae, and several analyses of evolution in the chordate and vertebrate lineages (e.g. [30–33]). Recent advances in DNA sequencing and assembly, coupled with the generation and processing of functional sequence datasets, notably RNA-Sequencing, which often result in hundreds of thousands of transcripts, highlight the need for a new generation of high-throughput computational analysis tools. Such tools are required to process: (i) large genomes; (ii) large numbers of genomes; (iii) large sets of genomic features, including large numbers of paralogous loci; but: (iv) without large computational efforts. Here we described Kraken, a novel computational method and software that fills this niche. Using the genomes of 12 fruit flies, we showed that the ability to translate indirectly, i.e. use an intermediate genome as a guide and then re-computing local alignments, comes at marginal cost in sensitivity, but at a substantial gain in computational efficiency: by removing the need to statically compute all-to-all synteny maps, Kraken scales linearly with the number of genomes and allows for the simultaneous analysis of dozens or even hundreds of genomes. We next evaluated Kraken on the well established and highly scrutinized genomes of human, mouse, rat, and showed that mapping orthologous sequences is highly accurate in predicting the precise boundaries of genomic features. This is particularly relevant for comparing protein-coding genes, since errors of even a single nucleotide can cause erroneous frame shifts and stop codons in open reading frames. Thus, Kraken can be used with ease to either create or improve comprehensive annotations through orthology for genomes that have little or no validated evidence within a few CPU hours, a functionality that is also provided by other, albeit more specialized software programs, such as RATT , GeneMapper , and HalLiftover .
To showcase Kraken’s utility for large-scale research projects aimed at discovering hitherto unexplored functional connections between orthologous members of gene families residing in different genomes, we re-analyzed a previously published large and comprehensive dataset consisting of RNA-Seq data from different tissues and multiple vertebrates . We emphasize that Kraken completed the analysis presented here within only a few hours of wall clock time and with minimal human involvement, yet yielding a rich set of results that are concordant with biological expectations and previous reports. In summary, we found that single-copy genes are more highly correlated across tissues and species than gene family members, which would indicate that single copy genes are enriched for fundamental cellular function essential to cells of different tissue types. By contrast, paralogs of gene families could have taken on more specialized roles, consistent with the concept of duplication and subsequent neo- and sub-functionalization . Moreover, orthologous gene family members are more highly correlated in brain tissues across species than their single-copy gene counterparts. This suggests that a number of genes, which were duplicated early in the vertebrate lineage, took on specialized functions in the brain, and were subsequently fixed in expression patterns, whereas we did not observe this consistently in the other tissues for which sequence was available. The pattern we see would fit with the theory that the complexity of the vertebrate brain and nervous system can in part be contributed to the redundancy of genes following the two whole genome duplications early in vertebrate evolution [36, 37]. Moreover, in three primates we found overlap of un-annotated transcribed regions, likely non-coding RNAs, with transcription levels most highly correlated across species in testis. Testis has been known to be the most actively transcribed tissue , and our results suggest that transcription does not occur in a random fashion. Long non-coding RNAs have recently been shown to take part in the circuitry controlling stem cell pluripotency and cell differentiation , consistent with expression in reproductive tissues, and indicating that the set of known RNAs is still an incomplete subset of the full inventory of all such transcribed loci.
In conclusion, we expect Kraken to dramatically reduce computational analysis time when deployed in future large-scale comparative studies. For a newly sequenced mammalian genome, for example, generating synteny maps to a single other mammal is sufficient for comparing it to dozens of others, at little extra computational cost. Moreover, the availability of second- and third-generation DNA and RNA sequencing technologies have made it possible to expand the set of reference genomes and expressed sequences into different branches of life. Successful and rapid analyses will thus require a computational framework that is easy to use and easy to set up.
Availability and requirements
Project Name: Kraken - A Universal Genomic Coordinate Translator for Comparative Genomics
Project home page: http://github.com/nedaz/kraken
Operating system(s): Linux
Programming Language: C++
License: Source code freely available under LPGL
Kmita M, Duboule D: Organizing axes in time and space; 25 years of colinear tinkering. Science. 2003, 301: 331-333.
Mallo M, Wellik DM, Deschamps J: Hox genes and regional patterning of the vertebrate body plan. Dev Biol. 2010, 344: 7-15.
Hoegg S, Meyer A: Hox clusters as models for vertebrate genome evolution. Trends Genet. 2005, 21: 421-424.
Meyer A: Hox gene variation and evolution. Nature. 1998, 391 (225): 227-228.
Vilella AJ, Severin J, Ureta-Vidal A, Heng L, Durbin R, Birney E: EnsemblCompara GeneTrees: complete, duplication-aware phylogenetic trees in vertebrates. Genome Res. 2009, 19: 327-335.
Catchen JM, Braasch I, Postlethwait JH: Conserved synteny and the zebrafish genome. Methods Cell Biol. 2011, 104: 259-285.
Jun J, Mandoiu II, Nelson CE: Identification of mammalian orthologs using local synteny. BMC Genomics. 2009, 10: 630-
Lindblad-Toh K, Garber M, Zuk O, Lin MF, Parker BJ, Washietl S, Kheradpour P, Ernst J, Jordan G, Mauceli E, Ward LD, Lowe CB, Holloway AK, Clamp M, Gnerre S, Alföldi J, Beal K, Chang J, Clawson H, Cuff J, Di Palma F, Fitzgerald S, Flicek P, Guttman M, Hubisz MJ, Jaffe DB, Jungreis I, Kent WJ, Kostka D, Lara M, et al: A high-resolution map of human evolutionary constraint using 29 mammals. Nature. 2011, 478: 476-482.
Harris RS: Improved pairwise alignment of genomic DNA. 2007, Ann Arbor: ProQuest, 84-
Kurtz S, Phillippy A, Delcher AL, Smoot M, Shumway M, Antonescu C, Salzberg SL: Versatile and open software for comparing large genomes. Genome Biol. 2004, 5: R12-
Soderlund C, Bomhoff M, Nelson WM: SyMAP v3.4: a turnkey synteny system with application to plant genomes. Nucleic Acids Res. 2011, 39: e68-
Grabherr MG, Russell P, Meyer M, Mauceli E, Alföldi J, Di Palma F, Lindblad-Toh K: Genome-wide synteny through highly sensitive sequence alignment: Satsuma. Bioinformatics. 2010, 26: 1145-1151.
Lyons E, Pedersen B, Kane J, Freeling M: The value of nonmodel genomes and an example using synmap within coge to dissect the hexaploidy that predates the rosids. Trop Plant Biol. 2008, 1: 181-190.
Paten B, Herrero J, Beal K, Fitzgerald S, Birney E: Enredo and Pecan: genome-wide mammalian consistency-based multiple alignment with paralogs. Genome Res. 2008, 18: 1814-1828.
Hickey G, Paten B, Earl D, Zerbino D, Haussler D: HAL: a hierarchical format for storing and analyzing multiple genome alignments. Bioinformatics. 2013, 29: 1341-1342.
Stark A, Lin MF, Kheradpour P, Pedersen JS, Parts L, Carlson JW, Crosby MA, Rasmussen MD, Roy S, Deoras AN, Ruby JG, Brennecke J, Hodges E, Hinrichs AS, Caspi A, Paten B, Park S-W, Han MV, Maeder ML, Polansky BJ, Robson BE, Aerts S, van Helden J, Hassan B, Gilbert DG, Eastman DA, Rice M, Weir M, Hahn MW, Park Y, et al: Discovery of functional elements in 12 Drosophila genomes using evolutionary signatures. Nature. 2007, 450: 219-232.
Brawand D, Soumillon M, Necsulea A, Julien P, Csárdi G, Harrigan P, Weier M, Liechti A, Aximu-Petri A, Kircher M, Albert FW, Zeller U, Khaitovich P, Grützner F, Bergmann S, Nielsen R, Pääbo S, Kaessmann H: The evolution of gene expression levels in mammalian organs. Nature. 2011, 478: 343-348.
Trapnell C, Roberts A, Goff L, Pertea G, Kim D, Kelley DR, Pimentel H, Salzberg SL, Rinn JL, Pachter L: Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nat Protoc. 2012, 7: 562-578.
Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q, Chen Z, Mauceli E, Hacohen N, Gnirke A, Rhind N, di Palma F, Birren BW, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A: Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011, 29: 644-652.
Zamani N, Sundström G, Höppner MP, Grabherr MG: Modular and configurable optimal sequence alignment software: cola. Source Code Biol Med. 2014, 9: 12-
Altschul S, Erickson B: Optimal sequence alignment using affine gap costs. Bull Math Biol. 1986, 48: 603-616.
Chao K-M, Pearson WR, Miller W: Aligning two sequences within a specified diagonal band. Bioinformatics. 1992, 8: 481-487.
Otto TD, Dillon GP, Degrave WS, Berriman M: RATT: rapid annotation transfer tool. Nucleic Acids Res. 2011, 39: e57-
Flicek P, Amode MR, Barrell D, Beal K, Brent S, Carvalho-Silva D, Clapham P, Coates G, Fairley S, Fitzgerald S, Gil L, Gordon L, Hendrix M, Hourlier T, Johnson N, Kähäri AK, Keefe D, Keenan S, Kinsella R, Komorowska M, Koscielny G, Kulesha E, Larsson P, Longden I, McLaren W, Muffato M, Overduin B, Pignatelli M, Pritchard B, Riat HS, et al: Ensembl 2012. Nucleic Acids Res. 2012, 40: D84-D90.
Khalil AM, Guttman M, Huarte M, Garber M, Raj A, Rivea Morales D, Thomas K, Presser A, Bernstein BE, van Oudenaarden A, Regev A, Lander ES, Rinn JL: Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression. Proc Natl Acad Sci U S A. 2009, 106: 11667-11672.
Guttman M, Donaghey J, Carey BW, Garber M, Grenier JK, Munson G, Young G, Lucas AB, Ach R, Bruhn L, Yang X, Amit I, Meissner A, Regev A, Rinn JL, Root DE: Lander ES: lincRNAs act in the circuitry controlling pluripotency and differentiation. Nature. 2011, 477: 295-300.
Muers M: Non-coding RNA: Zebrafish provide insight into lincRNA evolution. Nat Rev Genet. 2012, 13: 74-
Hoeppner MP, Lundquist A, Pirun M, Meadows JRS, Zamani N, Johnson J, Sundström G, Cook A, FitzGerald MG, Swofford R, Mauceli E, Torabi Moghadam B, Greka A, Alföldi A, Abouelleil A, Aftuck L, Bessette D, Berlin A, Brown A, Gearin G, Lui A, Macdonald JP, Pr GM: An improved canine genome and a comprehensive catalogue of coding genes and non-coding transcripts. PloS one. In Press
Kellis M, Birren BW, Lander ES: Proof and evolutionary analysis of ancient genome duplication in the yeast Saccharomyces cerevisiae. Nature. 2004, 428: 617-624.
Kasahara M, Naruse K, Sasaki S, Nakatani Y, Qu W, Ahsan B, Yamada T, Nagayasu Y, Doi K, Kasai Y, Jindo T, Kobayashi D, Shimada A, Toyoda A, Kuroki Y, Fujiyama A, Sasaki T, Shimizu A, Asakawa S, Shimizu N, Hashimoto S-I, Yang J, Lee Y, Matsushima K, Sugano S, Sakaizumi M, Narita T, Ohishi K, Haga S, Ohta F, et al: The medaka draft genome and insights into vertebrate genome evolution. Nature. 2007, 447: 714-719.
Nakatani Y, Takeda H, Kohara Y, Morishita S: Reconstruction of the vertebrate ancestral genome reveals dynamic genome reorganization in early vertebrates. Genome Res. 2007, 17: 1254-1265.
Putnam NH, Butts T, Ferrier DEK, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu J-K, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, et al: The amphioxus genome and the evolution of the chordate karyotype. Nature. 2008, 453: 1064-1071.
Alföldi J, Di Palma F, Grabherr M, Williams C, Kong L, Mauceli E, Russell P, Lowe CB, Glor RE, Jaffe JD, Ray DA, Boissinot S, Shedlock AM, Botka C, Castoe TA, Colbourne JK, Fujita MK, Moreno RG, ten Hallers BF, Haussler D, Heger A, Heiman D, Janes DE, Johnson J, de Jong PJ, Koriabine MY, Lara M, Novick PA, Organ CL, Peach SE, et al: The genome of the green anole lizard and a comparative analysis with birds and mammals. Nature. 2011, 477: 587-591.
Chatterji S, Pachter L: Reference based annotation with GeneMapper. Genome Biol. 2006, 7: R29-
Hahn MW: Distinguishing among evolutionary models for the maintenance of gene duplicates. J Hered. 2009, 100: 605-617.
Hoyle CHV: Evolution of neuronal signalling: transmitters and receptors. Auton Neurosci. 2011, 165: 28-53.
Cañestro C, Albalat R, Irimia M, Garcia-Fernàndez J: Impact of gene gains, losses and duplication modes on the origin and diversification of vertebrates. Semin Cell Dev Biol. 2013, 24: 83-94.
Schmidt EE: Transcriptional promiscuity in testes. Curr Biol. 1996, 6: 768-769.
We thank Lauren Solomon for designing the Kraken logo, UPPNEX/UPPMAX for providing computational resources, and the Bioinformatics Infrastructure for Life Sciences in Sweden (BILS). We thank Kerstin Lindblad-Toh, Leif Andersson, Jochen Wolf, and their respective groups for being early adopters of the Kraken technology.
This work was funded by a start-up grant from the Science for Life Laboratory (MGG), with support from the Bioinformatics Infrastructure for Life Sciences in Sweden (MPH, JD, and HL).
The authors declared that they have no competing interests.
NZ and MGG designed and developed the software; NZ, GS, MPH, JRSM, JD, HL, BJH, and MGG designed and performed the analyses. MPH, GS, JRSM, HL, and MGG provided the biological interpretation of the results. All authors wrote the manuscript.
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
About this article
Cite this article
Zamani, N., Sundström, G., Meadows, J.R. et al. A universal genomic coordinate translator for comparative genomics. BMC Bioinformatics 15, 227 (2014). https://doi.org/10.1186/1471-2105-15-227
- Comparative genomics
- Genomic coordinate translation
- Genomic duplication
- Cross-species gene expression analysis