- Open Access
Computational identification of genetic subnetwork modules associated with maize defense response to Fusarium verticillioides
© Kim et al. 2015
- Published: 1 December 2015
Maize, a crop of global significance, is vulnerable to a variety of biotic stresses resulting in economic losses. Fusarium verticillioides (teleomorph Gibberella moniliformis) is one of the key fungal pathogens of maize, causing ear rots and stalk rots. To better understand the genetic mechanisms involved in maize defense as well as F. verticillioides virulence, a systematic investigation of the host-pathogen interaction is needed. The aim of this study was to computationally identify potential maize subnetwork modules associated with its defense response against F. verticillioides.
We obtained time-course RNA-seq data from B73 maize inoculated with wild type F. verticillioides and a loss-of-virulence mutant, and subsequently established a computational pipeline for network-based comparative analysis.
Specifically, we first analyzed the RNA-seq data by a cointegration-correlation-expression approach, where maize genes were jointly analyzed with known F. verticillioides virulence genes to find candidate maize genes likely associated with the defense mechanism. We predicted maize co-expression networks around the selected maize candidate genes based on partial correlation, and subsequently searched for subnetwork modules that were differentially activated when inoculated with two different fungal strains. Based on our analysis pipeline, we identified four potential maize defense subnetwork modules. Two were directly associated with maize defense response and were associated with significant GO terms such as GO:0009817 (defense response to fungus) and GO:0009620 (response to fungus). The other two predicted modules were indirectly involved in the defense response, where the most significant GO terms associated with these modules were GO:0046914 (transition metal ion binding) and GO:0046686 (response to cadmium ion).
Through our RNA-seq data analysis, we have shown that a network-based approach can enhance our understanding of the complicated host-pathogen interactions between maize and F. verticillioides by interpreting the transcriptome data in a system-oriented manner. We expect that the proposed analytic pipeline can also be adapted for investigating potential functional modules associated with host defense response in diverse plant-pathogen interactions.
- Fusarium verticillioides
- host-pathogen interaction
- network-based analysis
- subnetwork module identification
Maize is one of the most significant crops in the world. Unfortunately maize is susceptible to a variety of pathogens, resulting in economic losses. Fusarium verticillioides is an important fungal pathogen causing maize ear and stalk diseases. More alarmingly, the fungus produces fumonisins, a group of toxic secondary metabolites harmful to animals and humans [1, 2]. Unfortunately, the development of effective management strategies for diseases has been stagnant due to a lack of basic understanding of this complicated host-pathogen interaction. The majority of, if not all, plant-microbe interactions that result in severe economic damages are cryptic and difficult to comprehend even with today's technological advancements. Plants respond to a variety of external stimuli, in particular microbial pathogens, with sophisticated response mechanisms, which we have recently started to gain a deeper understanding of. In contrast to the adaptive immune system found in animal systems, plant defense systems encode groups of genes to recognize and respond to specific pathogens [3, 4]. On the other hand, plant-associated microbes have coevolved with their hosts to overcome plant innate immunity and use a repertoire of effectors, enzymes and toxins to suppress host defense. Therefore, characterizing maize defense against F. verticillioides is critical for better comprehension of their mutual interactions as well as further enhancement of maize resistance.
Recently, the widespread application of high throughput technologies, such as microarray technology and next generation sequencing (NGS), has made a significant contribution to the study of host-pathogen interactions. In maize-pathogen interactions, several methods utilizing microarray data have focused on evaluating gene expression. For instance, Kelley et al.  identified maize genes involved in host resistance (or susceptibility) to pathogenic fungus Aspergillus flavus based on expression changes. Similarly, Campos-Bermudez et al.  identified maize genes and metabolites that showed expression variation after inoculation with F. verticillioides. Unfortunately, these analyses, as well as numerous other published studies, focused on expression differences in individual genes. For host-pathogen studies other than plant-pathogen, searching for host-pathogen gene pairs was performed through correlation analysis. Using Pearson's or Spearman rank correlation, Shea et al.  identified associated gene pairs in a human-bacterial system with Group A Streptococcus (GAS) and Reid et al.  identified molecular interactions between mouse and Plasmodium as well as mosquito and Plasmodium. In addition, Asters et al.  successfully constructed networks using Euclidean distance calculation based on their correlated gene pairs. While these approaches were successful in identifying correlated gene pairs and their networks in host-pathogen systems, improvements can be made with systematic investigation of cellular interactions or processes for underlying host-pathogen interactions. Network-based approaches [10–13] jointly analyzing gene expression data and protein-protein interaction (PPI) are receiving attention as better strategies for predicting biological markers or subnetwork modules. For example, Chuang et al.  predicted potential subnetwork modules associated with breast cancer metastasis using gene expression data as well as PPI network. Kim et al.  identified fungal virulence-associated subnetwork modules by seeking functionally coherent genes by using network-based comparative analysis.
In this study, our objective was to identify potential maize defense modules against the F. verticillioides in maize co-expression networks. We analyzed the RNA-seq data with the pipeline protocols and focused on the maize networks associated with maize-F. verticillioides interactions. Particularly, in addition to a wild type F. verticillioides, we used a F. verticillioides mutant, designated fsr1 strain, which shows a drastic reduction in fungal virulence . Our hypothesis is that the mutation in fsr1 gene disrupts downstream genetic networks that are crucial for maize-F. verticillioides interaction, and by analyzing differentially regulated subnetwork modules we will be able to identify important disease resistance mechanisms in maize. We also considered the dynamic changes in gene expression during maize-F. verticillioides interaction, and designed our study to collect RNA-seq data from three colonization phases: establishment of fungal infection (3 days post inoculation [dpi]), colonization and movement in vascular bundle (6 dpi) and host destruction and collapse (9 dpi). Prior to our computational analyses, we selected representative F. verticillioides virulence genes and searched for corresponding candidate maize genes that might be potentially associated with maize defense response. We used a cointegration-correlation expression approach to compare time-course expression patterns between maize and the selected F. verticillioides genes to obtain candidate maize genes. Based on the maize candidates, we predicted maize co-expression networks by partial correlation and searched subnetwork modules not only differentially expressed in the two conditions (i.e., wild type infected vs. the mutant infected conditions), but also composed of harmoniously coordinated genes. In this detection process, subnetwork modules were extended by the computationally efficient branch-out technique  with the probabilistic pathway activity inference . Based on the analysis, we identified potential maize subnetwork modules associated with maize defense response that were specifically defense-associated, well coordinated, and differently activated in the two conditions.
Overview of the proposed host-pathogen interaction analysis pipeline
Maize stalks were inoculated with F. verticillioides wild type and fsr1 mutant as previously described . We used the inbred line B73, a major source of commercial maize hybrids, that has no known stalk rot resistance, for this study. Maize stalk samples were collected 3, 6, and 9 dpi using manual sectioning, and scanned with fluorescence microscopy to identify host tissue damage and/or fungal colonization, particularly in the vascular bundles. These samples were dissected and collected separately for RNA extraction and cDNA synthesis following standard molecular biology procedures. For each sample subjected to sequencing, sectioning was performed on at least three stalk samples from each stage of infection, and isolated tissues were pooled for RNA extraction.
RNA sequencing and preprocessing
Figure 1A illustrates the first step of our analysis pipeline, where we preprocessed the RNA-seq data to obtain the normalized gene expression matrix. As previously described , RNA sequencing was processed using Illumina HiSeq 2000 producing sequencing results with relatively high quality and high coverage. In this sequencing, library preparation and RNA isolation were performed by Illumina's simplified sample prep kits and small RNA sample preparation kit, respectively. Also, Illumina HCS 1.515.1 and RTA 1.1348.0 also performed quality prefiltering, uncertainty assessment, base calling, and sequence cluster identification simultaneously. For F. verticillioides as well as maize, six independent sequencing libraries at three time points (i.e., 3 dpi, 6 dpi, and 9 dpi) for both wild type-inoculated and the mutant-inoculated samples, were obtained so that a total of 36 sample libraries were prepared. The prepared reads from the 36 libraries were mapped to the F. verticillioides strain 7600 reference genome  and to the maize B73 genome . The alignment was performed by Bowtie2  and another NGS data analysis tool called Sam-tools  was used to analyze the alignment result and obtain the read counts of all F. verticillioides genes as well as maize genes. In the alignment process, Bowtie2, optimized for gapped alignment and relatively longer reads, performed end-to-end mapping and extracted SAM format files by default mode. Next, filtering out genes with insignificant expression left 57% of F. verticillioides genes (8,072 genes) and 42.2% of maize genes (57,676 genes) for our subsequent analysis. During the filtering process, genes expressed in less than half of the total replicates, were eliminated. However, genes that were expressed only in one of the two conditions (i.e., wild type inoculated vs. mutant inoculated) were kept when they were expressed in more than 70% of the replicates in a given condition. This filtering process was performed to ensure that we retain potentially important differentially expressed genes while removing barely expressed genes. Finally, the filtered NGS data were normalized for relative quantification. For normalization, every read count across all replicates was normalized by the corresponding gene length. It is worth noting that, at this stage, the expression level was not normalized across different time points.
General statistics of the RNA-seq datasets analyzed in the current study
Wild type 6dpi
Type of run
Mean number of reads aligned
median depth of coverage
Mean number of reads aligned
median depth of coverage
Selection of pathogenicity genes of F. verticillioides
In order to narrow down maize genes to key candidates likely involved in maize defense mechanism, we selected representative F. verticillioides virulence genes whose expression patterns over time would be used as the criterion in the comparison. Among known F. verticillioides virulence genes, we selected the following four genes for our analysis after carefully considering their expression patterns, biochemical and physiological functions, as well as evolutionary conservation in other pathogenic fungi; i) FSR1 (FVEG 09767) - associated with fungal stalk rot virulence and sexual mating ; ii) FST1 (FVEG 08441) - associated with fungal growth and development particularly on maize ears ; iii) FvVE1 (FVEG 09521) - associated with aggressive pathogenesis and toxin production on maize seedlings ; iv) ZFR1 (FVEG 09648) - an important transcription factor controlling fungal growth and toxin biosynthesis on maize kernels . Once we established our preliminary network-based comparative analysis pipeline procedure, we were able to subsequently incorporate additional F. verticillioides virulence genes to improve the robustness of our prediction model.
Identification of maize subnetwork modules
Properties of the co-expression networks around the candidate maize genes (for maize inoculated with wild type F.verticillioides and maize inoculated with fsr1 mutant strain)
(cut-off partial correlation)
number of genes
number of interactions
number of genes
number of interactions
Probabilistic subnetwork activity inference
where µ1 and are the mean and the variance of the subnetwork activity level in one condition, and µ2 and are the mean and the variance of the subnetwork activity level in the other condition. n1 and n2 are the number of replicates (or independent measurements) in the respective conditions. For further details, readers are referred to , where the method was originally proposed in the context of cancer classification.
Characteristics of the candidate maize genes
Before predicting potential maize subnetwork modules involved in the maize defense response, we investigated whether our candidate maize genes obtained through the cointegration-correlation-expression analysis using the selected F. verticillioides virulence genes were possibly associated with maize defense. In order to examine whether the candidate maize genes were defense-associated, we compared them with the maize genes that corresponded to the F. veticillioides housekeeping genes, which are constitutively expressed and are mainly involved in the maintenance of fundamental cellular functions. For this comparison, four F. verticillioides housekeeping genes commonly used in molecular genetic studies were selected: the two beta-tubulin genes (FVEG 04081 and FVEG 05512), the pyruvate dehydrogenase E1 component subunit alpha gene (FVEG 07074), and the glyceraldehyde 3-phosphate dehydrogenase gene (FVEG 04927). For each F. verticillioides housekeeping gene, we searched for the corresponding maize genes through the cointegration-correlation-expression approach, and compared them against the candidate maize genes that were predicted to correspond to the four F. verticillioides virulence genes selected in our study. Both groups of F. verticillioides genes (housekeeping and virulence genes) were all relatively significantly expressed in all replicates, so one could presume that their expression patterns might be similar. However, the maize genes that were predicted to correspond to the F. verticillioides housekeeping genes and the candidate maize genes that corresponded to the F. verticillioides virulence genes overlapped only about 20% on average. Therefore, we concluded that the candidate maize genes that were identified by comparing maize genes against the selected F. verticillioides virulence genes using our proposed cointegration-correlation-expression approach were indeed very likely to be associated with the maize defense mechanism.
Identification of potential maize defense subnetwork modules
Potential maize subnetwork modules directly associated with maize defense response
Two potential maize subnetwork modules identified by the proposed network-based comparative analysis pipeline contained maize genes whose annotated GO terms were representative terms typically associated with responses to fungi. Figure 3 module-A contained three known maize genes, GRMZM2G001696 T01, GR-MZM2G374971 T01, and GRMZM5G870932 T01, associated with a significant GO term GO:0009817. For this GO:0009817, whose GO term is "defense response to fungus (incompatible interaction)", is defined as "a response of an organism to a fungus that prevents the occurrence or spread of disease". Benjamini-Hochberg FDR p-value of the most significant GO term, GO:0009817, for the Figure 3 module-A was 1.25e-06. Figure 3 module-B included three known maize genes, GRMZM2G001696 T01, GRMZM5G870932 T01, and GRMZM5G878558 T01, associated with a significant GO term, GO:0009620. This GO term is "response to fungus" and the definition is "any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a fungus"; the p-value of Benjamini-Hochberg FDR for this term was 5.69e-07. Note that both GO terms are directly related to the defense mechanism against fungal pathogens. It is also possible to view these two subnetwork modules as one whole module since the two modules shared two significant genes such as GRMZM2G001696 T01 and GRMZM5G870932 T01, however it is important to note that they were obtained from different seed genes and on different co-expression networks. This can be viewed as a demonstration of the reliability of the proposed network-based comparative analysis pipeline and its effectiveness in identifying potential maize subnetwork modules associated with maize defense mechanism against fungi.
Potential maize subnetwork modules indirectly involved in maize defense response
The other two potential maize subnetwork modules identified by our proposed pipeline were not directly associated with typical GO terms involved in defense response against fungal pathogens, but they also exhibited potential relevance to the maize defensive mechanism. Figure 4 module-A contained four known maize genes, GRMZM2G003930 T06, GRMZM2G056920 T03, GRMZM2G095025 T01, and GRMZM5G878558 T01, associated with GO term GO:0046914. This term GO:0046914 is for "transition metal ion binding" and had a p-value (for Benjamini-Hochberg FDR) of 4.56e-02. The GO term is described as "interacting selectively and non-covalently with a transition metal ions that is an element whose atom has an incompleted-subshell of extranuclear electrons, or which gives rise to a cation or cations with an incompleted-subshell". For module-B in Figure 4, two known maize genes, GRMZM2G001696 T01 and GRMZM2G085019 T01, were associated with GO:0046686. This GO term is for "response to cadmium ion" and is defined as "any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus". The p-value of Benjamini-Hochberg FDR for this GO term, GO:0046686, was 3.00e-02. For the two GO terms (GO:0046914 and GO:0046686), it is known that transition metals including cadmium (Cd) have a positive effect on plant defense system against the pathogenicity; hyperaccumulation of transition metals tends to reduce the growth of pathogens . Since both GO terms, i.e. GO:0046914 and GO:0046686, were all significantly related to transition metals, we can expect the two predicted maize subnetwork modules in Figure 4 to be potentially involved in maize defense system. For the four identified subnetwork modules in Figures 3 and 4, note that GO terms of the other genes other than the above-mentioned genes were either insignificant or not specified.
Finally, we investigated orthologous genes of those genes included in the two identified maize subnetwork modules in Figure 4. We looked for orthologous genes in Sorghum bicolor as well as Arabidopsis thaliana provided by RGAP (Rice Genome Annotation Project) website (http://rice.plantbiology.msu.edu). During this cross-check, we found orthologous genes of Figure 4 module-A member genes (i) involved in transition metal ion binding, such as AT2G01275, AT2G20030, AT4G28890, AT1G37130, and AT1G77760 for Arabidopsis thaliana and as SB03G007810, SB09G030900, SB04G024300, SB04G034470, and SB07G022750 for Sorghum bi-color and (ii) also associated with GO:0009610, "response to symbiotic fungus", such as SB04G024300, SB04G034470, and SB07G022750. Moreover, orthologs of the genes in Figure 4 module-B, such as AT1G59500, AT2G23170, and AT4G37390 for Arabidopsis thaliana as well as SB01G032020, SB02G038170, and SB03G035500 for Sorghum bicolor, were annotated with GO:0010279 "indole-3-acetic (IAA) acid amido synthetase activity". The "IAA amido synthetase" is known to be an important controller for plant defense system . This analysis based on the orthologous genes of the identified maize subnetwork modules in Figure 4 demonstrated that these two maize subnetwork modules may play potentially important roles in maize to defend itself from fungal pathogens.
In this paper, we proposed a network-based comparative RNA-seq data analysis pipeline specifically focusing on host-pathogen (F. verticillioides vs. maize) interactions. F. verticillioides is not only detrimental to the host plant maize but also to animals and humans, due to toxic secondary metabolites produced on infested commodities. To investigate their interactions, RNA-seq data from maize inbred B73 inoculated with two different F. verticillioides strains (wild type vs. fsr1 mutant) was prepared. In order to gain insight into the underlying biological functions and network interactions in maize defense response, we first filtered maize genes, using a cointegration-correlation-expression approach, to identify candidate genes whose activities corresponded to those of selected F. verticillioides virulence genes. Subsequently, we predicted the co-expression networks containing these maize candidate genes and searched for potential subnetwork modules likely to be associated with the maize defense mechanism. Based on our pipeline, we identified four potential maize subnetwork modules associated with the defense response against the F. verticillioides virulence. The member genes of the identified subnetwork modules showed relevance to defense-associated GO terms, well coordinated expression patterns with each other, and differential expression under the two different conditions (i.e., inoculation with wild type vs. fsr1 mutant). As shown in Figure 3, two of the identified maize subnetwork modules were directly associated with maize defense response against the F. verticillioides pathogenicity. The other two identified modules, shown in Figure 4, are also likely to be involved in the maize defense system, as they were predicted to be linked to accumulation of transition metals and defense response, and furthermore, as their member genes have orthologous genes in Sorghum bicolor and Arabidopsis thaliana that are associated with plant defense. Our results demonstrate that the proposed network-based analysis pipeline can improve our understanding of the biological mechanisms that underlie host-pathogen interactions, and that it has the potential to unveil novel genetic subnetwork modules specifically associated with plant defense response.
This research and the publication of this article was supported by the Agriculture and Food Research Initiative Competitive Grants Program (Grant number 2013-68004-20359) from the USDA National Institute of Food and Agriculture. This research was also supported by the NSF Award CCF-1149544 from the National Science Foundation and the Texas A&M AgriLife Research Genomics and Bioinformatics Seed Grant.
This article has been published as part of BMC Bioinformatics Volume 16 Supplement 13, 2015: Proceedings of the 12th Annual MCBIOS Conference. The full contents of the supplement are available online at http://www.biomedcentral.com/bmcbioinformatics/supplements/16/S13.
- Choi YE, Shim WB: Identification of genes associated with fumonisin biosynthesis in Fusarium verticillioides via proteomics and quantitative real-time PCR. J Microbiol Biotechnol. 2008, 18 (4): 648-657.PubMedGoogle Scholar
- Munkvold GP: Cultural and genetic approaches to managing mycotoxins in maize. Annu Rev Phytopathol. 2003, 41 (1): 99-116. 10.1146/annurev.phyto.41.052002.095510.View ArticlePubMedGoogle Scholar
- Chisholm ST, Coaker G, Day B, Staskawicz BJ: Host-microbe interactions: shaping the evolution of the plant immune response. Cell. 2006, 124 (4): 803-814. 10.1016/j.cell.2006.02.008.View ArticlePubMedGoogle Scholar
- Dodds PN, Rathjen JP: Plant immunity: towards an integrated view of plant-pathogen interactions. Nat Rev Genet. 2010, 11 (8): 539-548.View ArticlePubMedGoogle Scholar
- Kelley RY, Williams WP, Mylroie JE, Boykin DL, Harper JW, Windham GL, et al: Identification of maize genes associated with host plant resistance or susceptibility to Aspergillus flavus infection and aflatoxin accumulation. PLoS One. 2012, 7 (5): 36892-10.1371/journal.pone.0036892.View ArticleGoogle Scholar
- Campos-Bermudez VA, Fauguel CM, Tronconi MA, Casati P, Presello DA, Andreo CS: Transcriptional and metabolic changes associated to the infection by Fusarium verticillioides in maize inbreds with contrasting ear rot resistance. PLoS One. 2013, 8 (4): e61580-10.1371/journal.pone.0061580.View ArticlePubMedPubMed CentralGoogle Scholar
- Shea PR, Virtaneva K, Kupko JJ, Porcella SF, Barry WT, Wright FA, et al: Interactome analysis of longitudinal pharyngeal infection of cynomolgus macaques by group a streptococcus. Proc Natl Acad Sci U S A. 2010, 107 (10): 4693-4698. 10.1073/pnas.0906384107.View ArticlePubMedPubMed CentralGoogle Scholar
- Reid AJ, Berriman M: Genes involved in host-parasite interactions can be revealed by their correlated expression. Nucleic Acids Res. 2012, 41 (3): 1508-1518.View ArticlePubMedPubMed CentralGoogle Scholar
- Asters MC, Williams WP, Perkins AD, Mylroie JE, Windham GL, Shan X: Relating significance and relations of differentially expressed genes in response to Aspergillus flavus infection in maize. Scientific Reports. 2014, 4: 4815-View ArticlePubMedPubMed CentralGoogle Scholar
- Chuang HY, Lee E, Liu YT, Lee D, Ideker T: Network-based classification of breast cancer metastasis. Mol Syst Biol. 2007, 3: 140-View ArticlePubMedPubMed CentralGoogle Scholar
- Su J, Yoon BJ, Dougherty ER: Identification of diagnostic subnetwork markers for cancer in human protein-protein interaction network. BMC Bioinformatics. 2010, 11 (Suppl 6): S8-10.1186/1471-2105-11-S6-S8.View ArticlePubMedPubMed CentralGoogle Scholar
- Khunlertgit N, Yoon BJ: Simultaneous identification of robust synergistic subnetwork markers for effective cancer prognosis. EURASIP Journal on Bioinformatics and Systems Biology. 2014, 2014: 19-View ArticleGoogle Scholar
- Kim M, Zhang H, Woloshuk C, Shim WB, Yoon BJ: Computational prediction of pathogenic network modules in Fusarium verticillioides. IEEE/ACM Transactions on Computational Biology and Bioinformatics. 2015, PP (99):Google Scholar
- Shim WB, Sagaram US, Choi YE, So J, Wilkinson HH, Lee YW: FSR1 is essential for virulence and female fertility in Fusarium verticillioides and F. graminearum. Mol Plant Microbe Interact. 2006, 19 (7): 725-733. 10.1094/MPMI-19-0725.View ArticlePubMedGoogle Scholar
- Ma LJ, Van Der Does HC, Borkovich KA, Coleman JJ, Daboussi MJ, Di Pietro A, et al: Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium. Nature. 2010, 464 (7287): 367-373. 10.1038/nature08850.View ArticlePubMedPubMed CentralGoogle Scholar
- Schnable PS, Ware D, Fulton RS, Stein JC, Wei F, Pasternak S, et al: The B73 maize genome: complexity, diversity, and dynamics. Science. 2009, 326 (5956): 1112-1115. 10.1126/science.1178534.View ArticlePubMedGoogle Scholar
- Langmead B, Salzberg SL: Fast gapped-read alignment with Bowtie 2. Nat Methods. 2012, 9 (4): 357-359. 10.1038/nmeth.1923.View ArticlePubMedPubMed CentralGoogle Scholar
- Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, et al: The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009, 25 (16): 2078-2079. 10.1093/bioinformatics/btp352.View ArticlePubMedPubMed CentralGoogle Scholar
- Kim H, Woloshuk CP: Functional characterization of fst1 in Fusarium verticillioides during colonization of maize kernels. Mol Plant Microbe Interact. 2011, 24 (1): 18-24. 10.1094/MPMI-03-10-0074.View ArticlePubMedGoogle Scholar
- Myung K, Zitomer NC, Duvall M, Glenn AE, Riley RT, Calvo AM: The conserved global regulator VeA is necessary for symptom production and mycotoxin synthesis in maize seedlings by Fusarium verticillioides. Plant Pathol. 2012, 61 (1): 152-160. 10.1111/j.1365-3059.2011.02504.x.View ArticlePubMedPubMed CentralGoogle Scholar
- Bluhm BH, Kim H, Butchko RA, Woloshuk CP: Involvement of ZFR1 of Fusarium verticillioides in kernel colonization and the regulation of FST1, a putative sugar transporter gene required for fumonisin biosynthesis on maize kernels. Mol Plant Pathol. 2008, 9 (2): 203-211. 10.1111/j.1364-3703.2007.00458.x.View ArticlePubMedGoogle Scholar
- Engle RF, Granger CW: Co-integration and error correction: representation, estimation, and testing. Econometrica. 1987, 55 (2): 251-276. 10.2307/1913236.View ArticleGoogle Scholar
- Su J, Yoon BJ, Dougherty ER: Accurate and reliable cancer classification based on probabilistic inference of pathway activity. PLoS One. 2009, 4 (12): e8161-10.1371/journal.pone.0008161.View ArticlePubMedPubMed CentralGoogle Scholar
- Benjamini Y, Hochberg Y: Controlling the false discovery rate: a practical and powerful approach to multiple testing. Journal of the Royal Statistical Society. Series B (Methodological). 1995, 57 (1): 289-300.Google Scholar
- Reimand J, Arak T, Vilo J: g: Profiler - a web server for functional interpretation of gene lists (2011 update). Nucleic Acids Res. 2011, 39 (Web Server issue): W307-W315.View ArticlePubMedPubMed CentralGoogle Scholar
- Fones H, Davis CA, Rico A, Fang F, Smith JAC, Preston GM: Metal hyperaccumulation armors plants against disease. PLoS Pathogens. 2010, 6 (9): e1001093-10.1371/journal.ppat.1001093.View ArticlePubMedPubMed CentralGoogle Scholar
- Fu J, Liu H, Li Y, Yu H, Li X, Xiao J, Wang S: Manipulating broad-spectrum disease resistance by suppressing pathogen-induced auxin accumulation in rice. Plant Physiol. 2011, 155 (1): 589-602. 10.1104/pp.110.163774.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.