- Research article
- Open Access
Evolutionary sequence analysis of complete eukaryote genomes
BMC Bioinformatics volume 6, Article number: 53 (2005)
Gene duplication and gene loss during the evolution of eukaryotes have hindered attempts to estimate phylogenies and divergence times of species. Although current methods that identify clusters of orthologous genes in complete genomes have helped to investigate gene function and gene content, they have not been optimized for evolutionary sequence analyses requiring strict orthology and complete gene matrices. Here we adopt a relatively simple and fast genome comparison approach designed to assemble orthologs for evolutionary analysis. Our approach identifies single-copy genes representing only species divergences (panorthologs) in order to minimize potential errors caused by gene duplication. We apply this approach to complete sets of proteins from published eukaryote genomes specifically for phylogeny and time estimation.
Despite the conservative criterion used, 753 panorthologs (proteins) were identified for evolutionary analysis with four genomes, resulting in a single alignment of 287,000 amino acids. With this data set, we estimate that the divergence between deuterostomes and arthropods took place in the Precambrian, approximately 400 million years before the first appearance of animals in the fossil record. Additional analyses were performed with seven, 12, and 15 eukaryote genomes resulting in similar divergence time estimates and phylogenies.
Our results with available eukaryote genomes agree with previous results using conventional methods of sequence data assembly from genomes. They show that large sequence data sets can be generated relatively quickly and efficiently for evolutionary analyses of complete genomes.
The use of complete genomes for phylogenetic analysis has greatly improved our understanding of prokaryote evolution [1–3]. However, until recently, relatively few complete genome sequences were available for such analyses in eukaryotes. As this improves, there will be a greater demand on methodology for evolutionary analysis of complete genomes. Previous whole-genome studies of eukaryotes have focused on gene and gene family presence-absence [4–7], lineage-specific gene loss [8, 9], insertion-deletion markers and introns [6, 10, 11], and other non-sequence based information. While these approaches have their advantages, previous studies have not used complete genome sequences (nucleotides and/or amino acids) for reconstructing evolutionary relationships. At the same time, the complexity of eukaryote genomes, with numerous gene duplications and losses in different lineages, has created a challenge for sequence-based phylogeny estimation. Here, we outline a conservative approach designed to utilize the wealth of evolutionary information present in complete genome sequences by identifying orthologs in multiple eukaryotes for the purpose of evolutionary analysis.
Methods for the identification of clusters of orthologs and lineage-specific paralogs have proven useful for classifying gene function and identifying cases where genes have been differentially lost or duplicated in different lineages [12–14]. However, such assemblages of data contain a mixture of orthologs, paralogs, and missing data as a result of gene loss, and are not generally suitable for large-scale phylogenetic sequence analysis of organismal evolution. Our approach for comparing multiple genome sequences involves the identification of single-copy orthologs across a number of genomes for evolutionary analysis (Figure 1). We refer to such strict (1:1) orthologs as panorthologs, in reference to their presumed "complete" orthology, in contrast to synorthologs, which contain a mixture of species divergences and gene duplication events. In other words, panorthologs are those genes (or clusters of sequences) that contain only species divergences and do not contain in-paralogs, out-paralogs, or co-orthologs . On the other hand, synorthologs are those genes (or clusters of sequences) that contain species divergences and any combination of paralogy (in-paralogs and out-paralogs). While the use of panorthologs is conservative and reduces the number of usable genes or proteins, it also lowers the probability that errors will be made in confusing a species divergence with a gene duplication event. Because the ability to identify orthologs is diminished in analyses of small to moderate numbers of species or genomes, such a conservative method is appropriate in those cases. This conservative approach has been used to identify the number of shared, unduplicated proteins in Homo sapiens, Drosophila melanogaster, Caenorhabditis elegans, and Saccharomyces cerevisiae, where it was determined that such proteins perform primarily anabolic rather than catabolic functions .
We compare our phylogenetic results and divergence time estimates for an analysis of seventeen published eukaryote genomes to a previous study that assembled nuclear protein sequence data in a more conventional manner from public databases . While the phylogenetic relationships between the organisms included in this study are not controversial, with the exception of the position of nematodes , this general approach will prove useful as more genomes, including those with questionable phylogenetic affinity, are sequenced. In addition, this approach facilitates the estimation of divergence times between organisms with numerous molecular clock methods.
The number of orthologous clusters per pairwise comparison and the percentage of those clusters showing panorthology are presented in Table 1. On average, pairwise orthologous clusters contained approximately 60.3% panorthologs; exceptions include comparisons between fungi, including Encephalitozoon (average 89% panorthology), and all comparisons with Arabidopsis (average 34.6% panorthology). Comparisons within metazoans averaged 54.7% panorthology, with Mus and Rattus showing the highest number of shared transcripts (16,413 orthologous clusters; 79.2% panorthology) as expected due to their recent evolutionary divergence. Previous analyses showed approximately 12,400 panorthologs between Mus and Rattus . Caenorhabditis elegans and C. briggsae, who diverged roughly 100 Ma , also shared a large number of transcripts (12,416 orthologous clusters; 84.4% panorthology), which is similar to a previous estimate of 12,155 panorthologs . The number of orthologous clusters between Drosophila and Anopheles found here (7072, 61.3% panorthology) is also similar to a previous estimate of approximately 6130 panorthologs . Pairwise comparisons with the smallest genome, the Guillardia nucleomorph, averaged ~176 orthologous clusters, but the percentage of panorthologs varied greatly, from a low of 25.1% with Arabidopsis to as high as 97.2% with Encephalitozoon.
The intersection of nine metazoan genomes resulted in a large number of shared genes. Among the nine genomes, 285 panorthologs were found, totaling 97,581 amino acids. The neighbor-joining tree of that concatenation is shown in Figure 2; all nodes in this tree received 100% bootstrap support. The intersection of all seventeen eukaryote genomes included in this study resulted in three shared genes (t-complex protein delta subunit, proteasome beta type-1 subunit, and Nip7p biogenesis factor) and orthology was confirmed manually. The reconstructed trees for the three genes showed long-branch attraction errors associated with the intracellular parasite Encephalitozoon and the Guillardia nucleomorph (data not shown). This was expected because both have highly reduced genomes and high rates of substitution across many genes as a result of their current or ancestral parasitic and symbiotic lifestyles [23, 24]. For this reason, the intersection of the remaining fifteen genomes was determined, resulting in ten panorthologs. The intersection of genomes from twelve multicellular eukaryotes resulted in 63 panorthologs. The functional classifications of the panorthologs found here are similar to those identified in previous studies , with the most frequently represented functions being transcription, translation, replication and repair, and RNA processing. The phylogenetic trees reconstructed from the concatenated datasets both showed the expected relationships (Figure 3a and 3b) . All nodes in these trees received very high bootstrap support, with only one node showing less than 95% bootstrap support (animals + fungi in Figure 3a). The long branch observed in Plasmodium (Figure 3a) may be the result of both the long evolutionary separation from the other eukaryotes included in this study, and the high (A-T) composition of the genome  leading to biased amino acid compositions among proteins .
Phylogenetic trees were also reconstructed for each panortholog to test for congruence with well-supported phylogenies from the concatenated data (see Additional file 1). We found that in most cases, the consensus values calculated from individual trees agree with the high bootstrap support of the concatenated analysis. Two exceptions were the low consensus values for the accepted close relationship between animals and fungi and the contested position of nematodes. Both taxa showed slightly longer branch lengths, and long-branch attraction artifacts  may be affecting the individual datasets, causing low consensus values. Also, recent empirical  and simulation  studies suggest that results from multigene analyses are more accurate when a tree is derived from a concatenated dataset of individual genes rather than a consensus of trees from multiple analyses.
Divergence times were estimated for both the 15-genome and 12-genome datasets (Table 2). Results were consistent with previous studies [17, 30–33], showing an early divergence between plants, animals, and fungi (animals/fungi vs. plants ~1670 Ma, animals vs. fungi ~1400 Ma), and a Precambrian origin for animals (~900–1100 Ma). To specifically address the deuterostome-arthropod divergence within animals, two additional datasets were assembled to maximize the number of proteins analyzed: the intersection of seven genomes (Homo, Mus, Rattus, Takifugu, Drosophila, and Anopheles; Arabidopsis as outgroup) and the intersection of four genomes (Homo, Takifugu, and Drosophila; Arabidopsis as outgroup). The seven genome intersection contained 380 panorthologs (132,190 amino acids; Figure 4a), and yielded a vertebrate-arthropod divergence time of 955 Ma. The four genome intersection contained 753 panorthologs (287,000 amino acids; Figure 4b) and yielded a vertebrate-arthropod divergence time of ~1100 Ma. Although this last estimate was derived from more than five times the data previously used, the divergence time is remarkably consistent with previous large-scale studies , and suggests that bilaterian animals originated hundreds of millions of years before the first fossil evidence of their existence in the Cambrian (~520 Ma). With the exception of the maximum fossil-based time estimate used in the tetrapod-actinopterygian fish calibration, the other fossil constraints used here are minimums, and therefore the resulting time estimates are minimums . The agreement between our results and those of previous studies using different methods of data assembly suggests that our genome intersection approach is correctly assembling orthologs. Younger time estimates of the vertebrate-arthropod divergence have been obtained in some studies [35–37]. However, those results are problematic because they also contain estimates which are inconsistent (too young) with undisputed aspects of the fossil record, such as the oldest red algae (1200 Ma), green algae (1000 Ma), and stramenopiles (1000 Ma) [38–40]. Possible reasons for their inconsistency are discussed elsewhere .
The complete genome sequence of an organism is essentially the maximum amount of discrete, genetically encoded information available for evolutionary analyses. However, orthology determination has been a major obstacle in the analysis of complete genomes, especially in eukaryotes where considerable gene duplication and loss has created additional complexity. Our approach for evolutionary analysis of complete eukaryote genome sequences is both simple and fast compared with the conventional method of gene-by-gene orthology determination using similarity searches in the public databases. The results of this approach applied to a subset of the available eukaryote genomes show agreement with previous results using conventional (non-genomic) approaches. In addition, the relatively high consensus values for most nodes indicate general agreement in tree topology among individual panorthologs.
The relatively low number of common genes in our intersections of 12–17 genomes is a combination of using panorthologs and including distantly related species. Genes are more likely to duplicate over long periods of evolutionary time, as in the time elapsed since plants separated from animals (~1600 Ma) [17, 32]. Therefore, a better approach with such distantly related species (e.g., all eukaryotes), and those groups with high levels of gene duplication and gene loss (e.g., nematodes), would be to relax the orthology criterion and include synorthologs. In that case, a representative or consensus sequence may be chosen from among in-paralogs. On the other hand, the implementation used here (panorthologs) should yield many genes in analyses of genomes from closely related species (e.g., within mammals), even if large numbers of species are used.
The use of sequence data for comparative genomics and phylogenetics has several advantages over the use of datasets based on the presence and absence or position of genes, introns and insertions. Sequence data can provide a larger number of characters for analysis, yielding hundreds of thousands of amino acid sites and more than a million nucleotide sites in some cases. Also, statistical models of sequence change are better known than those for non-sequence based data. Finally, the assembly of sequence data from complete genomes of multiple organisms not only facilitates phylogenetic and divergence time analyses, but a diversity of other comparative evolutionary analyses requiring sequence information [42, 43].
Unlike previous studies of complete eukaryote genomes, here we have used a fast, conservative approach to assemble orthologous clusters of proteins for phylogenetic analysis and divergence time estimation. Our results are similar to previous studies that used conventional (slower) gene-by-gene data mining. We find that complete genome sequences support the close evolutionary relationship between animals and fungi, and also that molecular divergences between animals occurred approximately 400 million years before the Cambrian Explosion of fossils. Our approach will be further tested as more eukaryote genomes are sequenced.
Multigenome intersection approach for evolutionary analysis (MIA): Reciprocal BLAST  searches of genomes versus themselves and versus all other genomes included in the analysis were used to generate lists of pairwise similarity scores for each transcript. These scores were then used to generate orthologous clusters of proteins by first determining the "primary" ortholog pair through reciprocal best hits, then adding lineage-specific paralogs (in-paralogs) as implemented in INPARANOID . The settings used here (sequence overlap cut-off 50%, group overlap cut-off 50%, in-paralog confidence cut-off 5%) were considered optimal in the sense that all closely related lineage-specific paralogs (and alternative transcripts) will be placed in the same pairwise cluster, minimizing the probability that the same gene will be represented in more than one cluster. In-paralogs are added to clusters if they are more similar to one member of the primary ortholog pair than the two primary orthologs are to each other .
Only pairwise ortholog clusters can be obtained using INPARANOID. For phylogenetic analysis, ortholog sets for a larger number of genomes (at least four) must be constructed. Therefore, we combined the pairwise ortholog clusters from groups of species using a relational database. The intersection between ortholog clusters was determined by iteratively comparing sequence identification tags and reducing the intersected clusters at each round to exclude clusters that represent relationships other than panorthology. For example, consider genomes A, B and C. First, ortholog clusters are determined for each pairwise genome comparison, which results in clusters: A-B, B-C and A-C. Intersection of sets A-B and B-C is obtained by searching for common sequences of genome B in the two sets and merging the two sets accordingly into an ortholog cluster set of A-B-C. This combined set is reduced to include only clusters with panorthology relationships, i.e. only clusters with one sequence for each of the genomes A, B and C are retained. Further, the combined cluster A-B-C is compared with the pairwise cluster A-C based on common sequence tags for genome C. Any cluster from the combined A-B-C set that does not agree with the sequence grouping of genomes A and C is removed. This last step serves as an important check for orthology in each iteration of the intersection procedure, and is similar to the construction of three-member COGs (clusters of orthologous groups) . The steps described above were performed iteratively in order to add more species to the ortholog clusters. Any number of genomes can be intersected (tested up to seventeen here), and an outgroup can be treated as part of the intersection or added separately by using a pairwise comparison to one of the in-group taxa. All programming was written in Perl.
Analysis of Eukaryote Genomes: Complete protein transcripts were obtained for the following eukaryote genomes [three letter abbreviation]: Homo sapiens [Hsa] (version 34b.2) [16, 47, 48]; Mus musculus [Mmu] (version 32.2) [48, 49]; Rattus norvegicus [Rno] (version 3b.1) [19, 48]; Takifugu rubripes [Tru] (version 3.0) [50, 51]; Ciona intestionalis [Cin] (version 1.0) [51, 52]; Drosophila melanogaster [Dme] (version 3.1) [53, 54]; Anopheles gambiae [Aga] (version 2a.2) [48, 55]; Caenorhabditis elegans [Cel] (version 120) [56, 57]; Caenorhabditis briggsae [Cbr] (version 25) [21, 57]; Saccharomyces cerevisiae [Sce] [58, 59]; Neurospora crassa [Ncr] (version 3) [60, 61]; Ashbya gossypii [Ago] (version 1.0) [62, 63]; Encephalitozoon cuniculi [Ecu] [24, 64]; Arabidopsis thaliana [Ath] (version 5.0) [65, 66]; Cyanidoschyzon merolae [Cme] [67, 68]; Guillardia theta nucleomorph [Gtn] [23, 64]; and Plasmodium falciparum 3D7 [Pfa] (version 4.1) [25, 69]. Some genome transcripts were given unique sequence identifiers to avoid redundancy when sequence tags are truncated. The intersection of these seventeen genomes was determined as described above.
Each panortholog was aligned  and individual datasets were concatenated. Both individual panorthologs and concatenations were analyzed using maximum likelihood  to determine alpha parameters for the gamma rate-heterogeneity correction. Phylogenetic trees of concatenated datasets were reconstructed using neighbor-joining (Poisson + gamma correction model) with bootstrapping (2000 replicates)  and maximum likelihood (JTT + gamma correction model) with 1000 puzzling steps . Phylogenetic trees of individual datasets were reconstructed using maximum likelihood (Poisson + gamma correction model)  and a consensus tree was derived . Consensus values (i.e. the proportion of trees recovering a specific node) were calculated for each dataset.
Divergence times were estimated for concatenated datasets using Bayesian inference (JTT model)  as described previously . The following fossil dates were used as minimum constraints: Mus-Rattus 12 Ma , primate-rodent 65 Ma , tetrapod-actinopterygian fish 425 Ma (lower bound) and 495 Ma (upper bound) , vertebrate-urochordate 520 Ma , Drosophila-Anopheles 250 Ma , chordate-arthropod 545 Ma , green algae/plants-red algae 1200 Ma .
Wolf YI, Rogozin IB, Grishin NV, Tatusov RL, Koonin EV: Genome trees constructed using five different approaches suggest new major bacterial clades. BMC Evolutionary Biology 2001, 1: 8. 10.1186/1471-2148-1-8
Raymond J, Zhaxybayeva O, Gogarten JP, Gerdes SY, Blankenship RE: Whole-genome analysis of photosynthetic prokaryotes. Science 2002, 298: 1616–1620. 10.1126/science.1075558
Brochier C, Forterre P, Gribaldo S: Archaeal phylogeny based on proteins of the transcription and translation machineries: tackling the Methanopyrus kandleri paradox. Genome Biology 2004, 5: R17. 10.1186/gb-2004-5-3-r17
Korbel JO, Snel B, Huynen MA, Bork P: SHOT: a web server for the construction of genome phylogenies. Trends in Genetics 2002, 18: 158–162. 10.1016/S0168-9525(01)02597-5
House CH, Runnegar B, Fitz-Gibbon ST: Geobiological analysis using whole genome-based tree building applied to the Bacteria, Archaea, and Eukarya. Geobiology 2003, 1: 15–26. 10.1046/j.1472-4669.2003.00004.x
Wolf YI, Rogozin IB, Koonin EV: Coelomata and not Ecdysozoa: evidence from genome-wide phylogenetic analysis. Genome Research 2004, 14: 29–36. 10.1101/gr.1347404
Copley RR, Aloy P, Russell RB, Telford MJ: Systematic searches for molecular synapomorphies in model metazoan genomes give some support for Ecdysozoa after accounting for the idiosyncrasies of Caenorhabditis elegans . Evolution and Development 2004, 6: 164–169. 10.1111/j.1525-142X.2004.04021.x
Krylov DM, Wolf YI, Rogozin IB, Koonin EV: Gene loss, protein sequence divergence, gene dispensability, expression level, and interactivity are correlated in eukaryotic evolution. Genome Research 2003, 13: 2229–2235. 10.1101/gr.1589103
Hughes AL, Friedman R: Differential loss of ancestral gene families as a source of genomic divergence in animals. Proceedings of the Royal Society of London B: Biological Sciences 2004, 271: S107–109. 10.1098/rspb.2003.2556
Rogozin IB, Wolf YI, Sorokin AV, Mirkin BG, Koonin EV: Remarkable interkingdom conservation of intron positions and massive, lineage-specific intron loss and gain in eukaryotic evolution. Current Biology 2003, 13: 1512–1517. 10.1016/S0960-9822(03)00558-X
Coghlan A, Wolfe KH: Origins of recently gained introns in Caenorhabditis . PNAS 2004, 101: 11362–11367. 10.1073/pnas.0308192101
Lee Y, Sultana R, Pertea G, Cho J, Karamycheva S, Tsai J, Parvizi B, Cheung F, Antonescu V, White J, Holt I, Liang F, Quackenbush J: Cross-referencing eukaryotic genomes: TIGR Orthologous Gene Alignments (TOGA). Genome Research 2002, 12: 493–502. 10.1101/gr.212002
Li L, Stoeckert CJJ, Roos DS: OrthoMCL: identification of ortholog groups for eukaryotic genomes. Genome Research 2003, 13: 2178–2189. 10.1101/gr.1224503
Koonin EV, Fedorova ND, Jackson JD, Jacobs AR, Krylov DM, Makarova KS, Mazumder R, Mekhedov SL, Nikolskaya AN, Rao BS, Rogozin IB, Smirnov S, Sorokin AV, Sverdlov AV, Vasudevan S, Wolf YI, Yin JJ, Natale DA: A comprehensive evolutionary classification of proteins encoded in complete eukaryotic genomes. Genome Biology 2004, 5: R7. 10.1186/gb-2004-5-2-r7
Sonnhammer ELL, Koonin EV: Orthology, paralogy and proposed classification for paralog subtypes. Trends in Genetics 2002, 18: 619–620. 10.1016/S0168-9525(02)02793-2
Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W, Funke R, Gage D, Harris K, Heaford A, Howland J, Kann L, Lehoczky J, LeVine R, McEwan P, McKernan K, Meldrim J, Mesirov JP, Miranda C, Morris W, Naylor J, Raymond C, Rosetti M, Santos R, Sheridan A, Sougnez C, Stange-Thomann N, Stojanovic N, Subramanian A, Wyman D, Rogers J, Sulston J, Ainscough R, Beck S, Bentley D, Burton J, Clee C, Carter N, Coulson A, Deadman R, Deloukas P, Dunham A, Dunham I, Durbin R, French L, Grafham D, Gregory S, Hubbard T, Humphray S, Hunt A, Jones M, Lloyd C, McMurray A, Matthews L, Mercer S, Milne S, Mullikin JC, Mungall A, Plumb R, Ross M, Shownkeen R, Sims S, Waterston RH, Wilson RK, Hillier LW, McPherson JD, Marra MA, Mardis ER, Fulton LA, Chinwalla AT, Pepin KH, Gish WR, Chissoe SL, Wendl MC, Delehaunty KD, Miner TL, Delehaunty A, Kramer JB, Cook LL, Fulton RS, Johnson DL, Minx PJ, Clifton SW, Hawkins T, Branscomb E, Predki P, Richardson P, Wenning S, Slezak T, Doggett N, Cheng JF, Olsen A, Lucas S, Elkin C, Uberbacher E, Frazier M, Gibbs RA, Muzny DM, Scherer SE, Bouck JB, Sodergren EJ, Worley KC, Rives CM, Gorrell JH, Metzker ML, Naylor SL, Kucherlapati RS, Nelson DL, Weinstock GM, Sakaki Y, Fujiyama A, Hattori M, Yada T, Toyoda A, Itoh T, Kawagoe C, Watanabe H, Totoki Y, Taylor T, Weissenbach J, Heilig R, Saurin W, Artiguenave F, Brottier P, Bruls T, Pelletier E, Robert C, Wincker P, Smith DR, Doucette-Stamm L, Rubenfield M, Weinstock K, Lee HM, Dubois J, Rosenthal A, Platzer M, Nyakatura G, Taudien S, Rump A, Yang H, Yu J, Wang J, Huang G, Gu J, Hood L, Rowen L, Madan A, Qin S, Davis RW, Federspiel NA, Abola AP, Proctor MJ, Myers RM, Schmutz J, Dickson M, Grimwood J, Cox DR, Olson MV, Kaul R, Shimizu N, Kawasaki K, Minoshima S, Evans GA, Athanasiou M, Schultz R, Roe BA, Chen F, Pan H, Ramser J, Lehrach H, Reinhardt R, McCombie WR, de la Bastide M, Dedhia N, Blocker H, Hornischer K, Nordsiek G, Agarwala R, Aravind L, Bailey JA, Bateman A, Batzoglou S, Birney E, Bork P, Brown DG, Burge CB, Cerutti L, Chen HC, Church D, Clamp M, Copley RR, Doerks T, Eddy SR, Eichler EE, Furey TS, Galagan J, Gilbert JG, Harmon C, Hayashizaki Y, Haussler D, Hermjakob H, Hokamp K, Jang W, Johnson LS, Jones TA, Kasif S, Kaspryzk A, Kennedy S, Kent WJ, Kitts P, Koonin EV, Korf I, Kulp D, Lancet D, Lowe TM, McLysaght A, Mikkelsen T, Moran JV, Mulder N, Pollara VJ, Ponting CP, Schuler G, Schultz J, Slater G, Smit AF, Stupka E, Szustakowski J, Thierry-Mieg D, Thierry-Mieg J, Wagner L, Wallis J, Wheeler R, Williams A, Wolf YI, Wolfe KH, Yang SP, Yeh RF, Collins F, Guyer MS, Peterson J, Felsenfeld A, Wetterstrand KA, Patrinos A, Morgan MJ, Szustakowki J, de Jong P, Catanese JJ, Osoegawa K, Shizuya H, Choi S, Chen YJ: Initial sequencing and analysis of the human genome. Nature 2001, 409: 860–921. 10.1038/35057062
Hedges SB, Blair JE, Venturi ML, Shoe JL: A molecular timescale of eukaryote evolution and the rise of complex multicellular life. BMC Evolutionary Biology 2004, 4: 2. 10.1186/1471-2148-4-2
Blair JE, Ikeo K, Gojobori T, Hedges SB: The evolutionary position of nematodes. BMC Evolutionary Biology 2002, 2: 7. 10.1186/1471-2148-2-7
Gibbs RA, Weinstock GM, Metzker ML, Muzny DM, Sodergren EJ, Scherer S, Scott G, Steffen D, Worley KC, Burch PE, Okwuonu G, Hines S, Lewis L, DeRamo C, Delgado O, Dugan-Rocha S, Miner G, Morgan M, Hawes A, Gill R, Celera, Holt RA, Adams MD, Amanatides PG, Baden-Tillson H, Barnstead M, Chin S, Evans CA, Ferriera S, Fosler C, Glodek A, Gu Z, Jennings D, Kraft CL, Nguyen T, Pfannkoch CM, Sitter C, Sutton GG, Venter JC, Woodage T, Smith D, Lee HM, Gustafson E, Cahill P, Kana A, Doucette-Stamm L, Weinstock K, Fechtel K, Weiss RB, Dunn DM, Green ED, Blakesley RW, Bouffard GG, De Jong PJ, Osoegawa K, Zhu B, Marra M, Schein J, Bosdet I, Fjell C, Jones S, Krzywinski M, Mathewson C, Siddiqui A, Wye N, McPherson J, Zhao S, Fraser CM, Shetty J, Shatsman S, Geer K, Chen Y, Abramzon S, Nierman WC, Havlak PH, Chen R, Durbin KJ, Egan A, Ren Y, Song XZ, Li B, Liu Y, Qin X, Cawley S, Cooney AJ, D'Souza LM, Martin K, Wu JQ, Gonzalez-Garay ML, Jackson AR, Kalafus KJ, McLeod MP, Milosavljevic A, Virk D, Volkov A, Wheeler DA, Zhang Z, Bailey JA, Eichler EE, Tuzun E, Birney E, Mongin E, Ureta-Vidal A, Woodwark C, Zdobnov E, Bork P, Suyama M, Torrents D, Alexandersson M, Trask BJ, Young JM, Huang H, Wang H, Xing H, Daniels S, Gietzen D, Schmidt J, Stevens K, Vitt U, Wingrove J, Camara F, Mar Alba M, Abril JF, Guigo R, Smit A, Dubchak I, Rubin EM, Couronne O, Poliakov A, Hubner N, Ganten D, Goesele C, Hummel O, Kreitler T, Lee YA, Monti J, Schulz H, Zimdahl H, Himmelbauer H, Lehrach H, Jacob HJ, Bromberg S, Gullings-Handley J, Jensen-Seaman MI, Kwitek AE, Lazar J, Pasko D, Tonellato PJ, Twigger S, Ponting CP, Duarte JM, Rice S, Goodstadt L, Beatson SA, Emes RD, Winter EE, Webber C, Brandt P, Nyakatura G, Adetobi M, Chiaromonte F, Elnitski L, Eswara P, Hardison RC, Hou M, Kolbe D, Makova K, Miller W, Nekrutenko A, Riemer C, Schwartz S, Taylor J, Yang S, Zhang Y, Lindpaintner K, Andrews TD, Caccamo M, Clamp M, Clarke L, Curwen V, Durbin R, Eyras E, Searle SM, Cooper GM, Batzoglou S, Brudno M, Sidow A, Stone EA, Payseur BA, Bourque G, Lopez-Otin C, Puente XS, Chakrabarti K, Chatterji S, Dewey C, Pachter L, Bray N, Yap VB, Caspi A, Tesler G, Pevzner PA, Haussler D, Roskin KM, Baertsch R, Clawson H, Furey TS, Hinrichs AS, Karolchik D, Kent WJ, Rosenbloom KR, Trumbower H, Weirauch M, Cooper DN, Stenson PD, Ma B, Brent M, Arumugam M, Shteynberg D, Copley RR, Taylor MS, Riethman H, Mudunuri U, Peterson J, Guyer M, Felsenfeld A, Old S, Mockrin S, Collins F: Genome sequence of the Brown Norway rat yields insights into mammalian evolution. Nature 2004, 428: 493–521. 10.1038/nature02426
Coghlan A, Wolfe KH: Fourfold Faster Rate of Genome Rearrangement in Nematodes Than in Drosophila . Genome Res 2002, 12: 857–867. 10.1101/gr.172702
Stein LD, Bao Z, Blasiar D, Blumenthal T, Brent MR, Chen N, Chinwalla A, Clarke L, Clee C, Coghlan A, Coulson A, D'Eustachio P, Fitch DH, Fulton LA, Fulton RE, Griffiths-Jones S, Harris TW, Hillier LW, Kamath R, Kuwabara PE, Mardis ER, Marra MA, Miner TL, Minx P, Mullikin JC, Plumb RW, Rogers J, Schein JE, Sohrmann M, Spieth J, Stajich JE, Wei C, Willey D, Wilson RK, Durbin R, Waterston RH: The Genome Sequence of Caenorhabditis briggsae : A Platform for Comparative Genomics. PLoS Biology 2003, 1: E45. 10.1371/journal.pbio.0000045
Zdobnov EM, von Mering C, Letunic I, Torrents D, Suyama M, Copley RR, Christophides GK, Thomasova D, Holt RA, Subramanian GM, Mueller HM, Dimopoulos G, Law JH, Wells MA, Birney E, Charlab R, Halpern AL, Kokoza E, Kraft CL, Lai Z, Lewis S, Louis C, Barillas-Mury C, Nusskern D, Rubin GM, Salzberg SL, Sutton GG, Topalis P, Wides R, Wincker P, Yandell M, Collins FH, Ribeiro J, Gelbart WM, Kafatos FC, Bork P: Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster . Science 2002, 298: 149–159. 10.1126/science.1077061
Douglas S, Zauner S, Fraunholz M, Beaton M, Penny S, Deng LT, Wu X, Reith M, Cavalier-Smith T, Maier UG: The highly reduced genome of an enslaved algal nucleus. Nature 2001, 410: 1091–1096. 10.1038/35074092
Katinka MD, Duprat S, Cornillot E, Metenier G, Thomarat F, Prensier G, Barbe V, Peyretaillade E, Brottier P, Wincker P, Delbac F, El Alaoui H, Peyret P, Saurin W, Gouy M, Weissenbach J, Vivares CP: Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi . Nature 2001, 414: 450–453. 10.1038/35106579
Gardner MJ, Hall N, Fung E, White O, Berriman M, Hyman RW, Carlton JM, Pain A, Nelson KE, Bowman S, Paulsen IT, James K, Eisen JA, Rutherford K, Salzberg SL, Craig A, Kyes S, Chan MS, Nene V, Shallom SJ, Suh B, Peterson J, Angiuoli S, Pertea M, Allen J, Selengut J, Haft D, Mather MW, Vaidya AB, Martin DMA, Fairlamb AH, Fraunholz MJ, Roos DS, Ralph SA, McFadden GI, Cummings LM, Subramanian GM, Mungall C, Venter JC, Carucci DJ, Hoffman SL, Newbold C, Davis RW, Fraser CM, Barrell B: Genome sequence of the human malaria parasite Plasmodium falciparum . Nature 2002, 419: 498–511. 10.1038/nature01097
Pizzi E, Frontali C: Low-Complexity Regions in Plasmodium falciparum Proteins. Genome Res 2001, 11: 218–229. 10.1101/gr.GR-1522R
Felsenstein J: Cases in which parsimony or compatibility methods will be positively misleading. Syst Zool 1978, 27: 401–410.
Rokas A, Williams BL, King N, Carroll SB: Genome-scale approaches to resolving incongruence in molecular phylogenies. Nature 2003, 425: 798–804. 10.1038/nature02053
Gadagkar SR, Rosenberg MS, Kumar S: Inferring species phylogenies from multiple genes: Concatenated seqeunce tree versus consensus gene tree. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 2005, 304B: 64–74. 10.1002/jez.b.21026
Wray GA, Levinton JS, Shapiro LH: Molecular evidence for deep Precambrian divergences among metazoan phyla. Science 1996, 274: 568–573. 10.1126/science.274.5287.568
Feng DF, Cho G, Doolittle RF: Determining divergence times with a protein clock: update and reevaluation. Proc Nat Acad Sci U S A 1997, 94: 13028–13033. 10.1073/pnas.94.24.13028
Wang DY, Kumar S, Hedges SB: Divergence time estimates for the early history of animal phyla and the origin of plants, animals and fungi. Proceedings of the Royal Society of London B: Biological Sciences 1999, 266: 163–171. 10.1098/rspb.1999.0617
Nei M, Xu P, Glazko G: Estimation of divergence times from multiprotein sequences for a few mammalian species and several distantly related organisms. Proc Natl Acad Sci U S A 2001, 98: 2497–2502. 10.1073/pnas.051611498
Hedges SB, Kumar S: Precision of molecular time estimates. Trends in Genetics 2004, 20: 242–247. 10.1016/j.tig.2004.03.004
Peterson KJ, Lyons JB, Nowak KS, Takacs CM, Wargo MJ, McPeek MA: Estimating metazoan divergence times with a molecular clock. Proc Natl Acad Sci U S A 2004, 101: 6536–6541. 10.1073/pnas.0401670101
Douzery EJP, Snell EA, Bapteste E, Delsuc F, Philippe H: The timing of eukaryote evolution: Does a relaxed molecular clock reconcile proteins and fossils? Proc Natl Acad Sci U S A 2004, 101: 15386–15391. 10.1073/pnas.0403984101
Aris-Brosou S, Yang Z: Bayesian models of episodic evolution support a late precambrian explosive diversification of the Metazoa. Molecular Biology and Evolution 2003, 20: 1947–1954. 10.1093/molbev/msg226
Kumar S: Mesoproterozoic megafossil Chuaria-Tawuia association may represent parts of a multicellular plant, Vindhyan Supergroup, Central India. Precambrian Res 2001, 106: 187–211. 10.1016/S0301-9268(00)00093-0
Woods KN, Knoll AH, German TN: Xanthophyte Algae from the Mesoproterozoic/Neoproterozoic Transition: Confirmation and Evolutionary Implications. GSA Abstracts with Programs 1998, 30: A232.
Butterfield NJ: Bangiomorpha pubescens n. gen., n. sp.: Implications for the Evolution of Sex, Multicellularity, and the Mesoproterozoic/Neoproterozoic Radiation of Eukaryotes. Paleobiology 2000, 26: 386–404.
Blair JE, Hedges SB: Molecular Clocks Do Not Support the Cambrian Explosion. Mol Biol Evol 2005, 22: 387–390. 10.1093/molbev/msi039
Clark AG, Glanowski S, Nielsen R, Thomas PD, Kejariwal A, Todd MA, Tanenbaum DM, Civello D, Lu F, Murphy B, Ferriera S, Wang G, Zheng X, White TJ, Sninsky JJ, Adams MD, Cargill M: Inferring nonneutral evolution from human-chimp-mouse orthologous gene trios. Science 2003, 302: 1960–1963. 10.1126/science.1088821
Kumar S, Subramanian S: Mutation rates in mammalian genomes. Proc Natl Acad Sci U S A 2002, 99: 803–808. 10.1073/pnas.022629899
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997, 25: 3389–3402. 10.1093/nar/25.17.3389
Remm M, Storm CE, Sonnhammer EL: Automatic clustering of orthologs and in-paralogs from pairwise species comparisons. Journal of Molecular Biology 2001, 314: 1041–1052. 10.1006/jmbi.2000.5197
Tatusov RL, Galperin MY, Natale DA, Koonin EV: The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Research 2000, 28: 33–36. 10.1093/nar/28.1.33
Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, Sutton GG, Smith HO, Yandell M, Evans CA, Holt RA, Gocayne JD, Amanatides P, Ballew RM, Huson DH, Wortman JR, Zhang Q, Kodira CD, Zheng XH, Chen L, Skupski M, Subramanian G, Thomas PD, Zhang J, Gabor Miklos GL, Nelson C, Broder S, Clark AG, Nadeau J, McKusick VA, Zinder N, Levine AJ, Roberts RJ, Simon M, Slayman C, Hunkapiller M, Bolanos R, Delcher A, Dew I, Fasulo D, Flanigan M, Florea L, Halpern A, Hannenhalli S, Kravitz S, Levy S, Mobarry C, Reinert K, Remington K, Abu-Threideh J, Beasley E, Biddick K, Bonazzi V, Brandon R, Cargill M, Chandramouliswaran I, Charlab R, Chaturvedi K, Deng Z, Di Francesco V, Dunn P, Eilbeck K, Evangelista C, Gabrielian AE, Gan W, Ge W, Gong F, Gu Z, Guan P, Heiman TJ, Higgins ME, Ji RR, Ke Z, Ketchum KA, Lai Z, Lei Y, Li Z, Li J, Liang Y, Lin X, Lu F, Merkulov GV, Milshina N, Moore HM, Naik AK, Narayan VA, Neelam B, Nusskern D, Rusch DB, Salzberg S, Shao W, Shue B, Sun J, Wang Z, Wang A, Wang X, Wang J, Wei M, Wides R, Xiao C, Yan C, Yao A, Ye J, Zhan M, Zhang W, Zhang H, Zhao Q, Zheng L, Zhong F, Zhong W, Zhu S, Zhao S, Gilbert D, Baumhueter S, Spier G, Carter C, Cravchik A, Woodage T, Ali F, An H, Awe A, Baldwin D, Baden H, Barnstead M, Barrow I, Beeson K, Busam D, Carver A, Center A, Cheng ML, Curry L, Danaher S, Davenport L, Desilets R, Dietz S, Dodson K, Doup L, Ferriera S, Garg N, Gluecksmann A, Hart B, Haynes J, Haynes C, Heiner C, Hladun S, Hostin D, Houck J, Howland T, Ibegwam C, Johnson J, Kalush F, Kline L, Koduru S, Love A, Mann F, May D, McCawley S, McIntosh T, McMullen I, Moy M, Moy L, Murphy B, Nelson K, Pfannkoch C, Pratts E, Puri V, Qureshi H, Reardon M, Rodriguez R, Rogers YH, Romblad D, Ruhfel B, Scott R, Sitter C, Smallwood M, Stewart E, Strong R, Suh E, Thomas R, Tint NN, Tse S, Vech C, Wang G, Wetter J, Williams S, Williams M, Windsor S, Winn-Deen E, Wolfe K, Zaveri J, Zaveri K, Abril JF, Guigo R, Campbell MJ, Sjolander KV, Karlak B, Kejariwal A, Mi H, Lazareva B, Hatton T, Narechania A, Diemer K, Muruganujan A, Guo N, Sato S, Bafna V, Istrail S, Lippert R, Schwartz R, Walenz B, Yooseph S, Allen D, Basu A, Baxendale J, Blick L, Caminha M, Carnes-Stine J, Caulk P, Chiang YH, Coyne M, Dahlke C, Mays A, Dombroski M, Donnelly M, Ely D, Esparham S, Fosler C, Gire H, Glanowski S, Glasser K, Glodek A, Gorokhov M, Graham K, Gropman B, Harris M, Heil J, Henderson S, Hoover J, Jennings D, Jordan C, Jordan J, Kasha J, Kagan L, Kraft C, Levitsky A, Lewis M, Liu X, Lopez J, Ma D, Majoros W, McDaniel J, Murphy S, Newman M, Nguyen T, Nguyen N, Nodell M, Pan S, Peck J, Peterson M, Rowe W, Sanders R, Scott J, Simpson M, Smith T, Sprague A, Stockwell T, Turner R, Venter E, Wang M, Wen M, Wu D, Wu M, Xia A, Zandieh A, Zhu X: The sequence of the human genome. Science 2001, 291: 1304–1351. 10.1126/science.1058040
Ensembl Genome Browser: http://www.ensembl.org/.
Waterston RH, Lindblad-Toh K, Birney E, Rogers J, Abril JF, Agarwal P, Agarwala R, Ainscough R, Alexandersson M, An P, Antonarakis SE, Attwood J, Baertsch R, Bailey J, Barlow K, Beck S, Berry E, Birren B, Bloom T, Bork P, Botcherby M, Bray N, Brent MR, Brown DG, Brown SD, Bult C, Burton J, Butler J, Campbell RD, Carninci P, Cawley S, Chiaromonte F, Chinwalla AT, Church DM, Clamp M, Clee C, Collins FS, Cook LL, Copley RR, Coulson A, Couronne O, Cuff J, Curwen V, Cutts T, Daly M, David R, Davies J, Delehaunty KD, Deri J, Dermitzakis ET, Dewey C, Dickens NJ, Diekhans M, Dodge S, Dubchak I, Dunn DM, Eddy SR, Elnitski L, Emes RD, Eswara P, Eyras E, Felsenfeld A, Fewell GA, Flicek P, Foley K, Frankel WN, Fulton LA, Fulton RS, Furey TS, Gage D, Gibbs RA, Glusman G, Gnerre S, Goldman N, Goodstadt L, Grafham D, Graves TA, Green ED, Gregory S, Guigo R, Guyer M, Hardison RC, Haussler D, Hayashizaki Y, Hillier LW, Hinrichs A, Hlavina W, Holzer T, Hsu F, Hua A, Hubbard T, Hunt A, Jackson I, Jaffe DB, Johnson LS, Jones M, Jones TA, Joy A, Kamal M, Karlsson EK, Karolchik D, Kasprzyk A, Kawai J, Keibler E, Kells C, Kent WJ, Kirby A, Kolbe DL, Korf I, Kucherlapati RS, Kulbokas EJ, Kulp D, Landers T, Leger JP, Leonard S, Letunic I, Levine R, Li J, Li M, Lloyd C, Lucas S, Ma B, Maglott DR, Mardis ER, Matthews L, Mauceli E, Mayer JH, McCarthy M, McCombie WR, McLaren S, McLay K, McPherson JD, Meldrim J, Meredith B, Mesirov JP, Miller W, Miner TL, Mongin E, Montgomery KT, Morgan M, Mott R, Mullikin JC, Muzny DM, Nash WE, Nelson JO, Nhan MN, Nicol R, Ning Z, Nusbaum C, O'Connor MJ, Okazaki Y, Oliver K, Overton-Larty E, Pachter L, Parra G, Pepin KH, Peterson J, Pevzner P, Plumb R, Pohl CS, Poliakov A, Ponce TC, Ponting CP, Potter S, Quail M, Reymond A, Roe BA, Roskin KM, Rubin EM, Rust AG, Santos R, Sapojnikov V, Schultz B, Schultz J, Schwartz MS, Schwartz S, Scott C, Seaman S, Searle S, Sharpe T, Sheridan A, Shownkeen R, Sims S, Singer JB, Slater G, Smit A, Smith DR, Spencer B, Stabenau A, Stange-Thomann N, Sugnet C, Suyama M, Tesler G, Thompson J, Torrents D, Trevaskis E, Tromp J, Ucla C, Ureta-Vidal A, Vinson JP, Von Niederhausern AC, Wade CM, Wall M, Weber RJ, Weiss RB, Wendl MC, West AP, Wetterstrand K, Wheeler R, Whelan S, Wierzbowski J, Willey D, Williams S, Wilson RK, Winter E, Worley KC, Wyman D, Yang S, Yang SP, Zdobnov EM, Zody MC, Lander ES: Initial sequencing and comparative analysis of the mouse genome. Nature 2002, 420: 520–562. 10.1038/nature01262
Aparicio S, Chapman J, Stupka E, Putnam N, Chia J, Dehal P, Christoffels A, Rash S, Hoon S, Smit A, Gelpke MDS, Roach J, Oh T, Ho IY, Wong M, Detter C, Verhoef F, Predki P, Tay A, Lucas S, Richardson P, Smith SF, Clark MS, Edwards YJK, Doggett N, Zharkikh A, Tavtigian SV, Pruss D, Barnstead M, Evans C, Baden H, Powell J, Glusman G, Rowen L, Hood L, Tan YH, Elgar G, Hawkins T, Venkatesh B, Rokhsar D, Brenner S: Whole-Genome Shotgun Assembly and Analysis of the Genome of Fugu rubripes . Science 2002, 297: 1301–1310. 10.1126/science.1072104
DOE Joint Genome Institute: http://www.jgi.doe.gov/.
Dehal P, Satou Y, Campbell RK, Chapman J, Degnan B, De Tomaso A, Davidson B, Di Gregorio A, Gelpke M, Goodstein DM, Harafuji N, Hastings KE, Ho I, Hotta K, Huang W, Kawashima T, Lemaire P, Martinez D, Meinertzhagen IA, Necula S, Nonaka M, Putnam N, Rash S, Saiga H, Satake M, Terry A, Yamada L, Wang HG, Awazu S, Azumi K, Boore J, Branno M, Chin-Bow S, DeSantis R, Doyle S, Francino P, Keys DN, Haga S, Hayashi H, Hino K, Imai KS, Inaba K, Kano S, Kobayashi K, Kobayashi M, Lee BI, Makabe KW, Manohar C, Matassi G, Medina M, Mochizuki Y, Mount S, Morishita T, Miura S, Nakayama A, Nishizaka S, Nomoto H, Ohta F, Oishi K, Rigoutsos I, Sano M, Sasaki A, Sasakura Y, Shoguchi E, Shin-i T, Spagnuolo A, Stainier D, Suzuki MM, Tassy O, Takatori N, Tokuoka M, Yagi K, Yoshizaki F, Wada S, Zhang C, Hyatt PD, Larimer F, Detter C, Doggett N, Glavina T, Hawkins T, Richardson P, Lucas S, Kohara Y, Levine M, Satoh N, Rokhsar DS: The draft genome of Ciona intestinalis : insights into chordate and vertebrate origins. Science 2002, 298: 2157–2167. 10.1126/science.1080049
Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, Amanatides PG, Scherer SE, Li PW, Hoskins RA, Galle RF, George RA, Lewis SE, Richards S, Ashburner M, Henderson SN, Sutton GG, Wortman JR, Yandell MD, Zhang Q, Chen LX, Brandon RC, Rogers YH, Blazej RG, Champe M, Pfeiffer BD, Wan KH, Doyle C, Baxter EG, Helt G, Nelson CR, Gabor GL, Abril JF, Agbayani A, An HJ, Andrews-Pfannkoch C, Baldwin D, Ballew RM, Basu A, Baxendale J, Bayraktaroglu L, Beasley EM, Beeson KY, Benos PV, Berman BP, Bhandari D, Bolshakov S, Borkova D, Botchan MR, Bouck J, Brokstein P, Brottier P, Burtis KC, Busam DA, Butler H, Cadieu E, Center A, Chandra I, Cherry JM, Cawley S, Dahlke C, Davenport LB, Davies P, de Pablos B, Delcher A, Deng Z, Mays AD, Dew I, Dietz SM, Dodson K, Doup LE, Downes M, Dugan-Rocha S, Dunkov BC, Dunn P, Durbin KJ, Evangelista CC, Ferraz C, Ferriera S, Fleischmann W, Fosler C, Gabrielian AE, Garg NS, Gelbart WM, Glasser K, Glodek A, Gong F, Gorrell JH, Gu Z, Guan P, Harris M, Harris NL, Harvey D, Heiman TJ, Hernandez JR, Houck J, Hostin D, Houston KA, Howland TJ, Wei MH, Ibegwam C, Jalali M, Kalush F, Karpen GH, Ke Z, Kennison JA, Ketchum KA, Kimmel BE, Kodira CD, Kraft C, Kravitz S, Kulp D, Lai Z, Lasko P, Lei Y, Levitsky AA, Li J, Li Z, Liang Y, Lin X, Liu X, Mattei B, McIntosh TC, McLeod MP, McPherson D, Merkulov G, Milshina NV, Mobarry C, Morris J, Moshrefi A, Mount SM, Moy M, Murphy B, Murphy L, Muzny DM, Nelson DL, Nelson DR, Nelson KA, Nixon K, Nusskern DR, Pacleb JM, Palazzolo M, Pittman GS, Pan S, Pollard J, Puri V, Reese MG, Reinert K, Remington K, Saunders RD, Scheeler F, Shen H, Shue BC, Siden-Kiamos I, Simpson M, Skupski MP, Smith T, Spier E, Spradling AC, Stapleton M, Strong R, Sun E, Svirskas R, Tector C, Turner R, Venter E, Wang AH, Wang X, Wang ZY, Wassarman DA, Weinstock GM, Weissenbach J, Williams SM, WoodageT, Worley KC, Wu D, Yang S, Yao QA, Ye J, Yeh RF, Zaveri JS, Zhan M, Zhang G, Zhao Q, Zheng L, Zheng XH, Zhong FN, Zhong W, Zhou X, Zhu S, Zhu X, Smith HO, Gibbs RA, Myers EW, Rubin GM, Venter JC: The genome sequence of Drosophila melanogaster . Science 2000, 287: 2185–2195. 10.1126/science.287.5461.2185
Berkeley Drosophilia Genome Project[http://www.fruitfly.org]
Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, Nusskern DR, Wincker P, Clark AG, Ribeiro JM, Wides R, Salzberg SL, Loftus B, Yandell M, Majoros WH, Rusch DB, Lai Z, Kraft CL, Abril JF, Anthouard V, Arensburger P, Atkinson PW, Baden H, de Berardinis V, Baldwin D, Benes V, Biedler J, Blass C, Bolanos R, Boscus D, Barnstead M, Cai S, Center A, Chaturverdi K, Christophides GK, Chrystal MA, Clamp M, Cravchik A, Curwen V, Dana A, Delcher A, Dew I, Evans CA, Flanigan M, Grundschober-Freimoser A, Friedli L, Gu Z, Guan P, Guigo R, Hillenmeyer ME, Hladun SL, Hogan JR, Hong YS, Hoover J, Jaillon O, Ke Z, Kodira C, Kokoza E, Koutsos A, Letunic I, Levitsky A, Liang Y, Lin JJ, Lobo NF, Lopez JR, Malek JA, McIntosh TC, Meister S, Miller J, Mobarry C, Mongin E, Murphy SD, O'Brochta DA, Pfannkoch C, Qi R, Regier MA, Remington K, Shao H, Sharakhova MV, Sitter CD, Shetty J, Smith TJ, Strong R, Sun J, Thomasova D, Ton LQ, Topalis P, Tu Z, Unger MF, Walenz B, Wang A, Wang J, Wang M, Wang X, Woodford KJ, Wortman JR, Wu M, Yao A, Zdobnov EM, Zhang H, Zhao Q, Zhao S, Zhu SC, Zhimulev I, Coluzzi M, della Torre A, Roth CW, Louis C, Kalush F, Mural RJ, Myers EW, Adams MD, Smith HO, Broder S, Gardner MJ, Fraser CM, Birney E, Bork P, Brey PT, Venter JC, Weissenbach J, Kafatos FC, Collins FH, Hoffman SL: The genome sequence of the malaria mosquito Anopheles gambiae . Science 2002, 298: 129–149. 10.1126/science.1076181
The C. elegans Sequencing Consortium: Genome sequence of the nematode C. elegans : a platform for investigating biology. Science 1998, 282: 2012–2018. 10.1126/science.282.5396.2012
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG: Life with 6000 genes. Science 1996, 274: 546, 563–7. 10.1126/science.274.5287.546
Saccharomyces Genome Database[http://www.yeastgenome.org]
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B: The genome sequence of the filamentous fungus Neurospora crassa . Nature 2003, 422: 859–868. 10.1038/nature01554
Dietrich FS, Voegeli S, Brachat S, Lerch A, Gates K, Steiner S, Mohr C, Pohlmann R, Luedi P, Choi S, Wing RA, Flavier A, Gaffney TD, Philippsen P: The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome. Science 2004, 304: 304–307. 10.1126/science.1095781
Ashbya Genome Database[http://agd.unibas.ch]
European Bioinformatics Institute[http://www.ebi.ac.uk/]
The Arabidopsis Genome Initiative: Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 2000, 408: 796–815. 10.1038/35048692
The Institute for Genomic Research[http://www.tigr.org/]
Matsuzaki M, Misumi O, Shin IT, Maruyama S, Takahara M, Miyagishima SY, Mori T, Nishida K, Yagisawa F, Yoshida Y, Nishimura Y, Nakao S, Kobayashi T, Momoyama Y, Higashiyama T, Minoda A, Sano M, Nomoto H, Oishi K, Hayashi H, Ohta F, Nishizaka S, Haga S, Miura S, Morishita T, Kabeya Y, Terasawa K, Suzuki Y, Ishii Y, Asakawa S, Takano H, Ohta N, Kuroiwa H, Tanaka K, Shimizu N, Sugano S, Sato N, Nozaki H, Ogasawara N, Kohara Y, Kuroiwa T: Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature 2004, 428: 653–657. 10.1038/nature02398
Cyanidioschyzon merolae Genome Project[http://merolae.biol.s.u-tokyo.ac.jp]
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The CLUSTALX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 1997, 25: 4876–4882. 10.1093/nar/25.24.4876
Yang Z: PAML: a program package for phylogenetic analysis by maximum likelihood. Bioinformatics 1997, 13: 555–556.
Kumar S, Tamura K, Jakobsen I, Nei M: MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 2001, 17: 1244–1245. 10.1093/bioinformatics/17.12.1244
Strimmer K, von Haeseler A: Quartet Puzzling: A Quartet Maximum-Likelihood Method for Reconstructing Tree Topologies. Mol Biol Evol 1996, 13: 964–969.
Felsenstein J: Phylip: Phylogeny Inference Package. 3.6(a3) edition. Seattle, Department of Genome Sciences, University of Washington; 2002.
Kishino H, Thorne JL, Bruno WJ: Performance of a divergence time estimation method under a probabilistic model of rate evolution. Molecular Biology and Evolution 2001, 18: 352–361.
Jacobs LL, Downs WR: The evolution of murine rodents in Asia. In Rodent and Lagomorph Families of Asian Origins and Diversification. Volume 8. Edited by: Tomida Y, Li CK and Setoguchi T. Tokyo, National Science Museum Monographs; 1994:149–156.
Benton MJ: The Fossil Record 2. New York, Chapman & Hall; 1993.
Donoghue PCJ, Smith MP, Sansom IJ: The origin and early evolution of chordates: molecular clocks and the fossil record. In Telling the Evolutionary Time: Molecular Clocks and the Fossil Record. Edited by: Donoghue PCJ and Smith MP. New York, CRC Press; 2004:190–223.
Shu DG, Chen L, Han J, Zhang XL: An Early Cambrian tunicate from China. Nature 2001, 411: 472–473. 10.1038/35078069
Gaunt MW, Miles MA: An insect molecular clock dates the origin of the insects and accords with palaeontological and biogeographic landmarks. Molecular Biology and Evolution 2002, 19: 748–761.
The authors would like to thank Uthra Ramaswamy for additional computational support and Sankar Subramanian for helpful comments. This work was supported by grants to SBH from the National Science Foundation and the NASA Astrobiology Institute.
JEB and PS designed and developed the methodology. PS programmed the genome intersection software. JEB carried out the evolutionary analyses and drafted the manuscript. SBH coordinated the research and assisted with drafting the manuscript.
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Blair, J.E., Shah, P. & Hedges, S.B. Evolutionary sequence analysis of complete eukaryote genomes. BMC Bioinformatics 6, 53 (2005). https://doi.org/10.1186/1471-2105-6-53
- Complete Genome Sequence
- Evolutionary Analysis
- High Bootstrap Support
- Orthologous Cluster
- Eukaryote Genome