- Research article
- Open Access
Comparison of methods to detect copy number alterations in cancer using simulated and real genotyping data
© Mosén-Ansorena et al.; licensee BioMed Central Ltd. 2012
Received: 28 November 2011
Accepted: 30 June 2012
Published: 7 August 2012
The detection of genomic copy number alterations (CNA) in cancer based on SNP arrays requires methods that take into account tumour specific factors such as normal cell contamination and tumour heterogeneity. A number of tools have been recently developed but their performance needs yet to be thoroughly assessed. To this aim, a comprehensive model that integrates the factors of normal cell contamination and intra-tumour heterogeneity and that can be translated to synthetic data on which to perform benchmarks is indispensable.
We propose such model and implement it in an R package called CnaGen to synthetically generate a wide range of alterations under different normal cell contamination levels. Six recently published methods for CNA and loss of heterozygosity (LOH) detection on tumour samples were assessed on this synthetic data and on a dilution series of a breast cancer cell-line: ASCAT, GAP, GenoCNA, GPHMM, MixHMM and OncoSNP. We report the recall rates in terms of normal cell contamination levels and alteration characteristics: length, copy number and LOH state, as well as the false discovery rate distribution for each copy number under different normal cell contamination levels.
Assessed methods are in general better at detecting alterations with low copy number and under a little normal cell contamination levels. All methods except GPHMM, which failed to recognize the alteration pattern in the cell-line samples, provided similar results for the synthetic and cell-line sample sets. MixHMM and GenoCNA are the poorliest performing methods, while GAP generally performed better. This supports the viability of approaches other than the common hidden Markov model (HMM)-based.
We devised and implemented a comprehensive model to generate data that simulate tumoural samples genotyped using SNP arrays. The validity of the model is supported by the similarity of the results obtained with synthetic and real data. Based on these results and on the software implementation of the methods, we recommend GAP for advanced users and GPHMM for a fully driven analysis.
Two of the genetic instabilities associated with cancer are copy number alterations (CNAs) and loss of heterozygosity (LOH) events. Both are distinctive features of tumoural cells, and their acquisition has been reported to affect the expression of oncogenes and tumour-suppressor genes . Hence, the detection and characterization of both CNAs and LOH in tumoural samples is crucial to identify candidate cancer-related genes, as well as to discriminate cancer types  and to understand tumour initiation and complexity .
Single nucleotide polymorphism (SNP) arrays of Illumina  and Affymetrix  platforms allow screening for such alterations at high resolution and throughout the whole genome, providing measures of copy number changes and allelic ratio. Namely, the log R ratio (LRR) reflects the total intensity signals for both alleles, and the B allele frequency (BAF) is the relative proportion of one of the alleles with respect to the total intensity signal. Because they provide complementary information, both LRR and BAF signals are required for a complete characterization of copy number changes and allelic ratio. Yet, although each combination of copy number and allelic ratio has an expected LRR value and a specific BAF band pattern, these can be distorted by experimental probe-specific noise and by autocorrelated  and dye  biases, respectively.
In the case of tumour samples, three additional issues need to be considered. First, tumour genomes contain numerous altered regions whose copy number is different from two, making the genotypes in nearly the whole genome non-diploid. Under this situation of altered DNA index (i.e., half of the mean copy number), the LRR baseline level is shifted and needs to be estimated. Because this estimation affects copy number assignment [8, 9], equally likely results with different biological interpretations are possible. Second, tumour biopsies can be contaminated with normal cells whose genotypes are mainly diploid. This causes the LRR and BAF signals to shrink and converge towards those of a diploid state proportionally to the degree of contamination . Third, tumours can be composed of subclones, this is, subpopulations of cells that harbour specific alterations along with the shared ones, which makes LRR and BAF signals even more complex .
Characteristics of the six assessed methods
User-definable noise levels
User-definable LRR copy number means
Automatic LRR shift estimation
Automatic contamination estimation
Germline and somatic LOH distinction
Maximum copy number
HMM-based methods infer the most likely succession of genotypes (including copy number and allele distribution) given the LRR and BAF values under the assumption of certain conditions such as the expected LRR means for each copy number and the population B allele frequency (PFB) of each SNP. Sample-wide parameters, such as LRR baseline shift, standard deviation of the noise in both signals and proportion of normal cells, are unknown and typically optimized using expectation-maximization (EM) algorithms. The four aforementioned HMM-based methods differ on which parameters are optimized and how the EM is performed, as well as on the definition of the HMMs, including state characterization.
GAP is based on pattern recognition of a segmented and smoothed bi-dimensional profile. The method is implemented as a three-step workflow: (1) tQN (thresholded quantile normalization) normalization  for symmetrisation of BAF signal; (2) extraction of germline LOH regions, transformation of BAF into a unimodal symmetric signal (mBAF), segmentation of LRR and mBAF signals, and merging of LOH germline regions breakpoints with segmentation breakpoints; and (3) local copy number assignment through pattern recognition in the bi-dimensional LRR-BAF space. ASCAT transforms BAF into a unimodal symmetric signal, similar to mBAF, and performs a bivariate segmentation of LRR and the BAF transformation. Then, ASCAT assigns to each region the allele copy numbers that better fit the data, based on the ploidy that maximizes a reliability score.
Previous studies have compared some of these methods among themselves [9, 13] and against non-tumour-specific methods [12, 14], but so far no systematic assessment of the performance of these recently developed CNA and LOH detection approaches has been performed. Therefore, we have developed a comprehensive model that integrates normal cell contamination and intra-tumour heterogeneity to synthetically generate data that mimics tumoural samples on which to perform benchmarks. Using this synthetic data with a wide range of alteration characteristics and normal cell contamination levels, and a dilution series of a breast cancer cell-line , we have compared the performance of the six currently available software that consider normal cell contamination and rely on both BAF and LRR to detect CNAs and LOHs from unpaired samples on Illumina SNP arrays.
The model presented here draws from a model described by Yau et al.  that has been extended to integrate normal cell contamination and tumour heterogeneity. Furthermore, with the aim of adapting it to the generation of realistic tumour-like synthetic data, terms for known biases, generation restrictions and alteration variability have been included.
Where J is the number of cell types (one for normal cells plus J–1 tumour cell types with different copy numbers at locus i., W i,j is the proportion of the j-th cell type, is the expected LRR value for the copy number x of the j-th cell type present at locus i, the real number l is the sample-wide baseline shift, and c is an autocorrelated bias that simulates the background noise due to biological features that are not corrected for in the design of the array or in the detection methods.
Here, I is the indicator function and π is the proportion of BAF values that are forced to take the extreme homozygous values zero or one. The standard deviation σ baf_ho of homozygous values is set as a separate parameter from σ baf_he , given that it is usually observed to be lower.
The reason is that different alteration events yield certain possible genotype combinations. For example, given a diploid genotype AB, a CN4 (i.e. copy number 4) AABB genotype would not be coherent with a triplication of one of the alleles, but AAAB and ABBB would.
We implemented the described model as an R package whose purpose is the generation of synthetic SNP genotyping data. The software, named CnaGen, is available at http://web.bioinformatics.cicbiogune.es/cnagen and has fully customizable parameters as detailed below.
CnaGen allows to generate a broad range of region types combining different copy numbers, presence or not of somatic or germline LOH and spanning different number of SNPs per region. Furthermore, regions with intra-tumour complexity can also be generated establishing the copy number per subclone and the proportion of the major subclone. Regions can be combined in samples by establishing the region types to be present and the number of occurrences of each region type, and by defining the degree of normal cell contamination per sample. Other parameters, such as those defining LRR baseline shift, autocorrelated bias, expected LRR per copy number and noises in the LRR and BAF signals, are also user-definable.
As an alternative to fully specifying region characteristics, the user can provide a scaffold that contains them (length, copy number, allelic ratio and presence of germline LOH). Such scaffold, which implicitly holds the distribution of genomic rearrangements, may be obtained from previous experiments, so we regard this alternatively generated data as hybrid between synthetic and real. In this scenario, additional user-definable genome-wide properties are: long-distance genomic waves and overall level of intra-tumour complexity.
The following options to establish PFBs are available in CnaGen: (i) sampling from B allele frequencies in Caucasian populations of SNPs in the Illumina Human660W-Quad array; (ii) sampling from a uniform distribution; (iii) a constant p i =0.5, which maximizes genotype information; (iv) any other constant p i ; (v) and a three-peak distribution at 0, 0.5 and 1, which approximates PFBs in the human genome.
Generation of synthetic samples
In order to assess the performance of each method under different conditions, we generated synthetic SNP genotyping sample sets with CnaGen. The introduced statistical framework models the data observation process, which allows the assessment of the factor it comprises: copy number with the possibility of intra-tumour heterogeneity, presence of LOH, length of region and degree of normal cell contamination. Because the combinatorial space is too large to be explored exhaustively, subsets of values for each factor were selected. To test for the combined effect of number of copies, presence of LOH and length of alteration, fragments with copy numbers 1, 2, 3, 4 and 5 with and without somatic or germline LOH spanning 10, 20, 40, 80 or 160 SNPs were generated. Although longer aberrations, which may even comprise whole chromosomal arms, are typically found in tumoural cells, method performance does not change significantly beyond the longest considered length, specially under low normal cell contamination levels, as shown in the Results section. To test for the effect of different levels of normal cell contaminations, four percentages (0, 25, 50 or 75%) of non-tumoural cells were considered.
The rest of the parameters were set as follows: the LRR baseline shifts were drawn from Gaussian distributions with means established based on the correlation between DNA index and baseline shift (see "Parameter relationships" subsection below). In the Box-Jenkins model, the orders of the autoregressive and moving-average processes (i.e. p and τ ) were selected so that the resulting autocorrelated noise resembles the genomic curves found by Diskin et al. . Expected means, μx, for each copy number x were established as half of those values specified in  and approach those in the models of the methods evaluated. PFB values were drawn from those of the SNPs present in the Illumina Human660W-Quad array in Caucasian populations, and 30% of the homozygous probes were forced to take a zero or one value in the BAF signal in order to resemble real data. Finally, Gaussian noises were set to σ x =0.2 , σ baf_he =0.3 and σ baf_ho =0.15, being the former two similar to those HER2-positive samples of high quality analyzed by Li et al. ; and the noise for the homozygous SNPs in the BAF signal was set to half of the noise of the heterozygous SNPs, based on our own observations.
As a complimentary evaluation dataset, we used the dilution series of the CRL-2324 breast cancer cell line, genotyped with Illumina 370k BeadChips by Staaf et al.  where genomic DNA of breast carcinoma cells were mixed with DNA from lymphoblastoid cells at known proportions. This dataset has also been used by other authors [8, 9, 13] to assess the self-consistency of their methods.
In this dataset, the BAF and LRR signal noises range from 0.02 to 0.03 and from 0.18 to 0.25, respectively. These values are considered good in the case of BAF and on the limit for a sensitive analysis in the case of LRR . GC content bias was found to be between 0.016 and 0.042, which is satisfactory . Chromosomes 6 and 16 were removed from the analysis due to long heterozygous deletions in the lymphoblastoid cells [8, 9, 13] and sex chromosomes were not included, due to differences in how they are handled by the assessed methods.
Because GAP, GenoCNA and MixHMM do not integrate GC content biases into their models, the GC reduction model from the PennCNV package  was applied before running these programs on cell-line data. tQN , which accounts for dye bias, was only applied in GAP, given that it is part of the method workflow. Similarly, GenoCNA parameters are already adapted to signal asymmetry , avoiding the need for such normalization. In turn, no preprocessing was applied to the synthetic data, as it was generated without the known GC and dye biases, and GPHMM and OncoSNP, which do integrate the former, were fed a GC model with constant GC content values.
On synthetic data, a region overlapping approach was taken to define recalls and false discovery rates (FDR). An altered region is considered to be recalled when a call with the same copy number overlaps at least half of the length of such region. Other fractions were considered, but the difference in the results is small, as most calls overlap at least a 95% (not shown). In turn, due to the high density of alterations in the synthetic data, and tumoural samples in general, calls are likely to span more than one actual altered region, rendering the FDR an unsuitable measure for regions. Therefore, we used an approximation that consists on defining FDR as the fraction of alterations called with the wrong copy number. Methods with better breakpoint detection yield higher FDRs with such approach, so FDR are not comparable among methods. However, it is useful because it provides insight into how wrong calls are distributed among copy numbers and to what extend methods reduce the number of alteration calls as normal cell contamination increases.
On cell-line data, the set of alterations and their boundaries are unknown, so we evaluated the possibility of using self-consistency with respect to pure tumour calls in order to measure performance. However, we reckon that such measure does not reflect reality if the best performance is not expected to be good. It was thus decided to use a gold standard set of alterations, defined as a manually selected subset of alterations in the pure tumour sample detected by the best performing method over synthetic data. While on synthetic data we considered copy numbers up to 5, in the case of cell-line data, CNAs with copy number equal or greater than 4 were grouped  because of the limitations of GenoCNA and the uncertainty in high copy numbers of the gold standard set.
For the computation of recall rates and FDRs, normal diploid (i.e. heterozygous copy number 2) fragments were excluded from all method calls and the reference region sets in both the synthetic and real data assessments.
Results and discussion
LRR and BAF patterns in synthetic data
In general, the different copy numbers can easily be distinguished on both signals under null contamination, but, as contamination increases, BAF heterozygous bands converge towards 0.5 and LRR levels converge towards zero (LRR shift is disregarded for the sake of clarity). Given the same allelic imbalance pattern of the diploid region and the CNA with double duplication, the only way to distinguish them is the LRR signal, evidencing the need of these two signals for a correct genotype inference. The selected copy numbers and subclone proportions for the depicted complex CNAs yield rather simple band patterns in comparison to most cases. Even so, their BAF signals contain 4 and 6 heterozygous bands, which are so close that they blur into a single wide band even under low normal cell contamination. Besides, under high contamination levels, similar copy numbers are undistinguishable on the LRR signal. Only under unreal conditions of zero probe-specific and autocorrelated noises would some of these scenarios still be distinguishable in both signals (see Additional file 2).
Performance on synthetic samples
In general, normal cell contamination works against recall ability, but ASCAT, GPHMM and OncoSNP seem to perform better when there is some degree of contamination (Figure 3). In the case of ASCAT, the reason is that segmentation has low breakpoint sensitivity under null contamination, because the adequate number of breakpoints depends on the segmentation goodness of fit, which is better for lower normal cell contamination levels. Thus, except at heavy normal cell contamination levels, ASCAT outputs less breakpoints for a concrete sample if it contains less normal cells.
Tumoural cells typically present a DNA index greater than 1, and the expected LRR baseline value decreases below zero with the decreasing proportion of normal cells. Therefore, methods that keep the baseline fixed at zero will tend to assign lower copy numbers than the real ones under low normal cell contamination levels, and viceversa. This is the case for GenoCNA and MixHMM, which tend to make copy number 1, 2 and 3 calls under low, medium and high contamination levels respectively (Figure 3B). The lack of baseline shift estimation, and thus of ploidy, makes them perform poorly under any condition that moves away from near-diploidy and little contamination. However, although their performance is similar under little contamination, GenoCNA improves with respect to MixHMM as contamination increases, because the former does estimate normal cell contamination, in contrast to the latter. The rest of the methods estimate both LRR baseline shift and normal cell contamination, and present similar performance over the different copy numbers, except for their exceptional recall rates for copy number 1 alterations and the fact that they tend to make few copy number 2 calls and prefer to assign regions copy numbers 1 and 3. GPHMM presents another exception to such trend. It has the most stable performance over different contamination levels, which is coherent with the results reported by Li et al. However, such stability and self-consistency arises from its tendency to make copy number 3 calls, a preference that is shared with ASCAT. This is visible if we separate recall rates by copy number and also if we look at the overall performance by dataset, as they perform better over samples with more copy number 3 alterations, specially near-triploid samples.
As expected, recall rates are higher for longer regions (Additional file 4). At low normal cell contamination levels, recall rates do not seem to improve much for regions beyond 160 SNPs in length but, at 75% contamination, regions need to be longer in order to be called correctly. Yet, given the observed trend in recall rates by length, we do not expect recall rates to significantly vary beyond lengths of a few hundred SNPs. The greater difference is seen on shorter regions, where GAP is more sensitive than the rest of the methods.
GAP is by far the best performer under the range of tested conditions and alterations. One of the advantages of GAP is that it puts breakpoint sensitivity before specificity. Then, it merges similar segments if necessary. This way, it is less probable for changes in mean to be missed. Additionally, ploidy estimation based on the whole segmented data, a feature that GAP shares with ASCAT, proves to give better results than the expectation maximisation performed by their HMM-based counterparts. Surprisingly, the updated version of GAP, in addition to having a worse performance than its older release, has a clear problem at the pattern recognition step under both null and high normal cell contamination levels, where the recognition fails in most of the samples. This is seen in its graphical output and reflected on the overall recall performance.
If we regard correct calls as those that match not only copy number but also LOH status, methods manage to keep around 90% of the correct calls, with the greatest recall drop being 13.2% for ASCAT under null contamination (see Additional file 5). Nevertheless, the recalling behaviour is similar to the case where only copy number is required to consider calls correct. Therefore, LOH status recalling is similar to the recalling ability seen in the overall performance.
Complementary to the recall rates, we investigated FDRs and how wrong calls are distributed among copy numbers (see Additional file 6). GPHMM and OncoSNP tend to call higher copy numbers than the real ones, whereas MixHMM and GenoCNA clearly do the opposite. Furthermore, as contamination increases, the calls of these latter two have a bias towards copy number 3, as also seen in Figure 3B. The reduction in their FDRs at higher contamination levels does not mean better specificity. Instead, it reflects the fact that these methods decrease the number of alteration calls, probably due to the high uncertainty under heavy contamination levels.
Performance on hybrid samples
In order to assess whether (i) the spatial distribution of real genomic rearrangements and (ii) the inclusion of long regions result in different method performances from what is observed on purely synthetic data, we generated hybrid samples based on scaffolds taken from GAP's output  over three real tumour cell-line samples: BLC_B1_T17 (near-diploid), L_B1_T24B (high complexity) and MDA_468 (LOH-rich). Similarly to their fully synthetic counterparts, the samples were generated at 0, 25, 50 and 75% normal cell contamination levels (more details in Additional file 7).
In comparison to the tests on synthetic data, we observed slightly better performance in GPHMM and worse in OncoSNP and ASCAT (see Additional file 8). Nevertheless, GAP remained as the best performer, and MixHMM and GenoCNA as the worst. Furthermore, observations made using purely synthetic data on the effect of alteration pattern, LRR baseline shift and normal cell contamination also apply.
Performance on cell-line samples
Given its superior and stable performance over synthetic data, the GAP output on the pure tumour sample was selected as the gold standard for the performance assessment over the cell-line data. There are additional reasons for the selection of GAP: (i) its ability to generate a visual output, which aids in the task of manual adjustment, also available, (ii) the fact that it is open source, which enables manual fine-tuning, and (iii) its confidence scores in the calls. After visual assessment of the output, calls were filtered to leave only those with maximum confidence in a scale from 1 to 4 and a minimum length of 40 SNPs, the length at which GAP recall rates under null contamination start to stabilize on synthetic data (Additional file 4). A total of 261 regions out of the original 367 were left. Only 12 of them were copy number 1, so results regarding this copy number were not expected to be reliable. Grouping recall rates by copy number and contamination level, we observed that, in general, methods behave on cell-line data similarly to synthetic data. Specifically, the samples are near-diploid with many copy number-neutral LOH regions and copy number 3 and 4 alterations, so we expected similar results to those with the synthetic LOH-enriched and near-triploid samples.
The greater approximation of ASCAT and updated GAP calls to the GAP reference in comparison to GAP calls on non-pure samples validates the gold standard approach we took, based on the results from the best method over the pure tumour sample.
In , the reduction of signal that is lost proportionally to the level of normal cell contamination is modelled as a separate parameter q, the coefficient of contraction. However, such parameter only makes sense under experimental variability, given its actual theoretical linear relationship with normal contamination. As expected, a linear regression of these two parameters in the samples analyzed by Popova et al.  shows a high correlation (R²=0.8, R²=0.9 without one outlier) (see Additional file 10), where the coefficient of LRR contraction is approximately half of the level of tumour purity (q=0.49(1-p)). The definition of our synthetic data automatically generates this correlation.
Given that DNA index is linearly affected by normal cell contamination, the relationship between baseline shift and contamination described by Li et al.  can be redefined in terms of DNA index against baseline shift. Using the breast cancer samples profiled by Popova et al. , we compared the DNA index and the baseline shift obtained by GPHMM  and found a high correlation (R²=0.89) (see Additional file 10). The baseline shift for our synthetic samples was thus established based on this correlation.
Depending on the estimation of the baseline shift, different and plausible biological solutions may be obtained for a sample, and it has been stated that alternative experiments, such as fluorescence in situ hybridization (FISH), are required in such cases [12–14]. We reckon that a sensible integration of the aforementioned relationships into future methods' models can aid on the restriction of possible solutions. Still, because of the convolution of tumour subclones, in presence of normal cell contamination, regions with 2 or more tumour subclones cannot be uniquely genotyped  with current SNP array technology.
The model implemented in the software CnaGen, which integrates normal cell contamination and intra-tumour heterogeneity, has proven to generate synthetic samples that mimic the characteristic BAF and LRR signal patterns of real tumour samples. Supporting this, synthetic, hybrid and cell-line data reach similar outcomes in the assessment of the performance of the methods tested in this work.
When it comes to selecting a method to detect germline copy number variations (CNVs), where the additional tumour-related issues do not apply, some authors consider that it is better to use several algorithms and compare data , while others consider that this approach might not be appropriate . Given the results of the present study, we consider that the safest option would be to carefully choose a good method that is adequate for the characteristics of the data and knowledge of the researcher. For example, in our case, GAP is the best method, outperforming the rest in nearly all situations, followed by ASCAT and GPHMM, even though this latter and OncoSNP present a more sustained performance at heavy contamination levels. Yet, because the success of a method does not solely depend on performance, but also on the interaction between the user and the software implementation, GAP and ASCAT may not be suitable for users without basic programming skills, who may prefer the easy to use GPHMM. Thus, bearing in mind performance, parameterization and ease of use, we recommend GAP for advanced users and GPHMM for a fully driven analysis.
DMA is supported by the Government of Navarra, Spain through the grant "Ayuda predoctoral para realizar una tesis doctoral y obtener el título de doctor (Plan de Formación y de I+D 2010/2011)". NRE and AMA are supported by the Department of Industry, Tourism and Trade of the Government of the Autonomous Community of the Basque Country (Etortek Research Programs 2009/2011) and from the Innovation Technology Department of the Bizkaia County.
- Pinkel D, Segraves R, Sudar D, Clark S, Poole I, Kowbel D, Collins C, Kuo WL, Chen C, Zhai Y, et al.: High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays. Nature Genet 1998, 20(2):207–211. 10.1038/2524View ArticlePubMedGoogle Scholar
- Beroukhim R, Mermel CH, Porter D, Wei G, Raychaudhuri S, Donovan J, Barretina J, Boehm JS, Dobson J, Urashima M, et al.: The landscape of somatic copy-number alteration across human cancers. Nature 2010, 463(7283):899–905. 10.1038/nature08822PubMed CentralView ArticlePubMedGoogle Scholar
- Notta F, Mullighan CG, Wang JC Y, Poeppl A, Doulatov S, Phillips L, Ma J, Minden MD, Downing JR, Dick JE: Evolution of human BCR-ABL1 lymphoblastic leukaemia-initiating cells. Nature 2011, 471(7337):254–254.View ArticleGoogle Scholar
- Illumina,. http://www.illumina.com
- Affymetrix,. http://www.affymetrix.com
- Diskin SJ, Li M, Hou C, Yang S, Glessner J, Hakonarson H, Bucan M, Maris JM, Wang K: Adjustment of genomic waves in signal intensities from whole-genome SNP genotyping platforms. Nucleic Acids Res 2008, 36(19):e126-e126. 10.1093/nar/gkn556PubMed CentralView ArticlePubMedGoogle Scholar
- Staaf J, Vallon-Christersson J, Lindgren D, Juliusson G, Rosenquist R, Höglund M, Borg Å, Ringnér M: Normalization of Illumina Infinium whole-genome SNP data improves copy number estimates and allelic intensity ratios. BMC Bioinform 2008, 9(1):409–409. 10.1186/1471-2105-9-409View ArticleGoogle Scholar
- Popova T, Manié E, Stoppa-Lyonnet D, Rigaill G, Barillot E, Stern MH: Genome Alteration Print (GAP): a tool to visualize and mine complex cancer genomic profiles obtained by SNP arrays. Genome Biol 2009, 10(11):R128-R128. 10.1186/gb-2009-10-11-r128PubMed CentralView ArticlePubMedGoogle Scholar
- Yau C, Mouradov D, Jorissen RN, Colella S, Mirza G, Steers G, Harris A, Ragoussis J, Sieber O, Holmes CC: A statistical approach for detecting genomic aberrations in heterogeneous tumor samples from single nucleotide polymorphism genotyping data. Genome Biol 2010, 11(9):R92-R92.PubMed CentralPubMedGoogle Scholar
- Staaf J, Lindgren D, Vallon-Christersson J, Isaksson A, Göransson H, Juliusson G, Rosenquist R, Höglund M, Borg A, Ringnér M: Segmentation-based detection of allelic imbalance and loss-of-heterozygosity in cancer cells using whole genome SNP arrays. Genome Biol 2008, 9(9):R136-R136. 10.1186/gb-2008-9-9-r136PubMed CentralView ArticlePubMedGoogle Scholar
- Parisi F, Ariyan S, Narayan D, Bacchiocchi A, Hoyt K, Cheng E, Xu F, Li P, Halaban R, Kluger Y: Detecting copy number status and uncovering subclonal markers in heterogeneous tumor biopsies. BMC Genomics 2011, 12(1):230–230. 10.1186/1471-2164-12-230PubMed CentralView ArticlePubMedGoogle Scholar
- Sun W, Wright F, Tang Z, Nordgard SH, Van Loo P, Yu T, Kristensen VN, Perou CM: Integrated study of copy number states and genotype calls using high-density SNP arrays. Nucleic Acids Res 2009, 37(16):5365–5377. 10.1093/nar/gkp493PubMed CentralView ArticlePubMedGoogle Scholar
- Li A, Liu Z, Lezon-Geyda K, Sarkar S, Lannin D, Schulz V, Krop I, Winer E, Harris L, Tuck D: GPHMM: an integrated hidden Markov model for identification of copy number alteration and loss of heterozygosity in complex tumor samples using whole genome SNP arrays. Nucleic Acids Res 2011, 39(12):4928–4941. 10.1093/nar/gkr014PubMed CentralView ArticlePubMedGoogle Scholar
- Liu Z, Li A, Schulz V, Chen M, Tuck D: MixHMM: Inferring Copy Number Variation and Allelic Imbalance Using SNP Arrays and Tumor Samples Mixed with Stromal Cells. PLoS ONE 2010, 5(6):14–14.Google Scholar
- Van Loo P, Nordgard SH, Lingjærde OC, Russnes HG, Rye IH, Sun W, Weigman VJ, Marynen P, Zetterberg A, Naume B, et al.: Allele-specific copy number analysis of tumors. Proc Nat Acad Sci U S Am 2010, 107(39):16910–16915. 10.1073/pnas.1009843107View ArticleGoogle Scholar
- Chen H, Xing H, Zhang NR: Estimation of parent specific DNA copy number in tumors using high-density genotyping arrays. PLoS Comput Biol 2011, 7(1):e1001060-e1001060. 10.1371/journal.pcbi.1001060PubMed CentralView ArticlePubMedGoogle Scholar
- Attiyeh EF, Diskin SJ, Attiyeh MA, Mossé YP, Hou C, Jackson EM, Kim C, Glessner J, Hakonarson H, Biegel JA, et al.: Genomic copy number determination in cancer cells from single nucleotide polymorphism microarrays based on quantitative genotyping corrected for aneuploidy. Genome Res 2009, 19(2):276–283.PubMed CentralView ArticlePubMedGoogle Scholar
- Rasmussen M, Sundstrom M, Goransson Kultima H, Botling J, Micke P, Birgisson H, Glimelius B, Isaksson A: Allele-specific copy number analysis of tumor samples with aneuploidy and tumor heterogeneity. Genome Biol 2011, 12(10):R108-R108. 10.1186/gb-2011-12-10-r108PubMed CentralView ArticlePubMedGoogle Scholar
- Wang K, Li M, Hadley D, Liu R, Glessner J, Grant SF, Hakonarson H, Bucan M: PennCNV: an integrated hidden Markov model designed for high-resolution copy number variation detection in whole-genome SNP genotyping data. Genome Res 2007, 17(11):1665–1674. 10.1101/gr.6861907PubMed CentralView ArticlePubMedGoogle Scholar
- Winchester L, Yau C, Ragoussis J: Comparing CNV detection methods for SNP arrays. Brief Funct Genomics Proteomics 2009, 8(5):353–366. 10.1093/bfgp/elp017View ArticleGoogle Scholar
- Zhang D, Qian Y, Akula N, Alliey-Rodriguez N, Tang J, Gershon ES, Liu C: Accuracy of CNV Detection from GWAS Data. PLoS ONE 2011, 6(1):9–9.Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.